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The map label for this gene is gyrB [H]

Identifier: 120596837

GI number: 120596837

Start: 3952

End: 6369

Strand: Direct

Name: gyrB [H]

Synonym: Sputw3181_0004

Alternate gene names: 120596837

Gene position: 3952-6369 (Clockwise)

Preceding gene: 120596836

Following gene: 120596838

Centisome position: 0.08

GC content: 45.91

Gene sequence:

>2418_bases
ATGTCAGAGAATAGTTACGATTCTTCGAGTATTAAGGTCCTTAAGGGCCTTGATGCAGTACGTAAGAGACCTGGGATGTA
TATTGGTGACACCGACGACGGTACGGGTCTACATCATATGGTGTTCGAAGTAGTCGATAACTCTATCGATGAAGCGTTAG
CCGGCCATTGTAGCGATATTACCATTACTATCCATGTAGATGGTTCTGTTTCTGTAAAAGATGATGGGCGTGGTATTCCG
GTCGCTATCCATGAGGAAGAAGGCGTATCGGCAGCAGAAGTTATTATGACGGTATTACATGCCGGCGGTAAGTTCGATGA
TAACTCCTATAAAGTGTCTGGTGGTCTCCACGGCGTGGGTGTTTCGGTTGTAAACGCTTTATCTCGAAAGCTGCAAATGA
CTATTCGTCGTGCTGGCAAAGTGTACGAACAATTTTATACCCATGGTGTGCCTGATGCGCCAATCAAAGAGATTGGTGAT
GCAACCAAAACAGGGACTGAAATCCGTTTTTGGCCGAGTGAAGATACCTTTAGTGATGTTGAATTTCACTTTGAAATTCT
AGCAAAACGGGTGCGTGAATTGTCATTCCTTAACTCTGGTGTCGGTATTCGCCTGATTGATGAACGTGACAATAAAAATG
AATTTTTCAAATATGAAGGCGGTATTAGTGCATTCGTCGATTATTTGAACCGTAATAAAACACCTGTCAATAAAGAGGTG
TTCCACTTTATGCAAGAGCGTGACGACGGTATTACCGTTGAAGTGGCTATGCAGTGGAACGATGGCTATCAAGAAAATAT
TTTCTGTTTTACCAACAACATTCCGCAACGTGATGGTGGTACTCACTTAGTTGGTTTCCGTAGTGCGTTGACGCGTAACC
TCAACAACTACATGGAAAACGAAGGCTATAACAAGAAAGGTAAGACGAACGCGACCGGCGATGATGCCCGTGAAGGCTTG
ACTGCCGTTATCTCTGTTAAAGTGCCAGATCCTAAGTTCAGCTCGCAAACCAAAGACAAACTGGTTTCTAGTGAAGTGAA
AACCGCGGTTGAACAAACCATGGGTGAGAAGCTGAACGATTATCTACTGGAAAATCCCGCCGATGCTAAGCTGATTGTCG
GTAAGATTGTCGATGCGGCCCGTGCCCGTGAAGCGGCGCGTAAAGCCCGTGAAATGACCCGTCGTAAAGGCGCACTCGAT
TTAGGTGGTTTGCCAGGTAAGCTGGCCGATTGCCAAGAAAAAGATCCTGGTCTTTCCGAAATTTACATAGTGGAAGGGGA
CTCTGCTGGCGGTAGCGCTAAGCAGGGACGTAACCGTAAGAACCAAGCGATTCTGCCACTAAAGGGTAAAATTCTAAACG
TTGAGAAAGCCCGCTTCGATAAGATGTTGTCTTCTCAAGAAGTGGCGACGCTTATCACGGCGCTTGGCTGTGGTATTGGT
CGCGACGAATATAACCCAGATAAAACCCGTTATCACAACATCATCATCATGACGGATGCTGACGTCGATGGCTCGCACAT
TCGTACCTTGCTGTTAACCTTCTTCTTCCGTCAAATGCCTGAATTGATTGAACGTGGTTATGTGTATATTGCTCAGCCGC
CTTTGTTTAAAGTGAAAAAGGGTAAGCAAGAACAGTATTTGAAAGACGAACCAGCACTGACTCAATACCTCACGACTCAA
GCATTGGATGGCACCGTAGTGTATCCAACGCAAGGTGCACCTGGTATGTCGGGTGAGCCGTTAGAGCGTTTAGTGACTCA
GTACCGTGAAGTAGAGGCGATTGTTGCCCGTTTAGAGCAGCGTTATCCAACGCACATTACTAACCGTATGCTTTATCACC
CAATGATCACCAACGAGATGTTGGCTGATGAAGGTAAGATGAAAGAGTGGATCGATGCCTTTATCCACGAGCTGGTGGAA
ATGGAAAGTAGCGGTGTATTGTATTCGGCTGAGCCAATTCTCGATCCTGAGCGTAAAGTGTATCTGCCTAAGATCACTAT
TCGTAAACACGGTATCGATACTAACTACCTGTTTACTTATGATTTCTTCCAATCAAGCGATTATCAACGTATCGGCAAGT
TAGGTGCGGCGTTAGATGGCATGATTGAAGAAGGCGGCTATGTACAACGTGGTGAACGGGTTAAAGAAGTCGGCAGTTTC
CTCGAAGCGCTAGATTGGACGATTTCGGAAGCGAAACGTGGCTTGTATATCCAGCGTTATAAAGGATTGGGTGAGATGAA
CCCTGAGCAATTGTGGGAAACGACAATGGATCCTGAATCACGCCGTATGTTGCAAGTCACAATTGATGATGCCGTGGGTG
CCGATCAGTTGTTCACTTGTTTGATGGGTGACCAAGTGGAACCACGCCGTGAGTTTATCGAAGCGAACGCATTGAACGTG
GCTAACTTAGACGTGTAA

Upstream 100 bases:

>100_bases
CCTGCTGCAATAGTCGATTCGTTACACACGCCTCCCAATAGGATGTTTCATGTGGAACAGGGACGTGTAACGGTAGTTGA
ATAACCATTGAGAGAATAAT

Downstream 100 bases:

>100_bases
TGTGTTGTAATGATTGATTGAATTAAGGGAAAGCTGAGGCTTTCCCTTTTTGTTTGGATTATTAATACTTATCTCATAAA
AGCAGCTATTATGTTGTTAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDITITIHVDGSVSVKDDGRGIP
VAIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSRKLQMTIRRAGKVYEQFYTHGVPDAPIKEIGD
ATKTGTEIRFWPSEDTFSDVEFHFEILAKRVRELSFLNSGVGIRLIDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKEV
FHFMQERDDGITVEVAMQWNDGYQENIFCFTNNIPQRDGGTHLVGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKTAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEPALTQYLTTQ
ALDGTVVYPTQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTHITNRMLYHPMITNEMLADEGKMKEWIDAFIHELVE
MESSGVLYSAEPILDPERKVYLPKITIRKHGIDTNYLFTYDFFQSSDYQRIGKLGAALDGMIEEGGYVQRGERVKEVGSF
LEALDWTISEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRREFIEANALNV
ANLDV

Sequences:

>Translated_805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDITITIHVDGSVSVKDDGRGIP
VAIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSRKLQMTIRRAGKVYEQFYTHGVPDAPIKEIGD
ATKTGTEIRFWPSEDTFSDVEFHFEILAKRVRELSFLNSGVGIRLIDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKEV
FHFMQERDDGITVEVAMQWNDGYQENIFCFTNNIPQRDGGTHLVGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKTAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEPALTQYLTTQ
ALDGTVVYPTQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTHITNRMLYHPMITNEMLADEGKMKEWIDAFIHELVE
MESSGVLYSAEPILDPERKVYLPKITIRKHGIDTNYLFTYDFFQSSDYQRIGKLGAALDGMIEEGGYVQRGERVKEVGSF
LEALDWTISEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRREFIEANALNV
ANLDV
>Mature_804_residues
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDITITIHVDGSVSVKDDGRGIPV
AIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSRKLQMTIRRAGKVYEQFYTHGVPDAPIKEIGDA
TKTGTEIRFWPSEDTFSDVEFHFEILAKRVRELSFLNSGVGIRLIDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKEVF
HFMQERDDGITVEVAMQWNDGYQENIFCFTNNIPQRDGGTHLVGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGLT
AVISVKVPDPKFSSQTKDKLVSSEVKTAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDL
GGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEPALTQYLTTQA
LDGTVVYPTQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTHITNRMLYHPMITNEMLADEGKMKEWIDAFIHELVEM
ESSGVLYSAEPILDPERKVYLPKITIRKHGIDTNYLFTYDFFQSSDYQRIGKLGAALDGMIEEGGYVQRGERVKEVGSFL
EALDWTISEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRREFIEANALNVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=566, Percent_Identity=25.0883392226148, Blast_Score=124, Evalue=4e-28,
Organism=Homo sapiens, GI19913406, Length=574, Percent_Identity=23.8675958188153, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=70.6102117061021, Blast_Score=1184, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=548, Percent_Identity=39.7810218978102, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=552, Percent_Identity=25.3623188405797, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=507, Percent_Identity=23.8658777120316, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=165, Percent_Identity=32.1212121212121, Blast_Score=71, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=587, Percent_Identity=25.2129471890971, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136538, Length=547, Percent_Identity=26.508226691042, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90096; Mature: 89965

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDI
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
TITIHVDGSVSVKDDGRGIPVAIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSRKLQMTIRRAGKVYEQFYTHGVPDAPIKEIGDATKTGTEIRFWPSEDTFSDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHH
EFHFEILAKRVRELSFLNSGVGIRLIDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKEV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQERDDGITVEVAMQWNDGYQENIFCFTNNIPQRDGGTHLVGFRSALTRNLNNYMEN
HHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKTAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEPALTQYLTTQALDGTVVYPTQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTHITNRMLYHPMITNEMLADEGKMKEWIDAFIHELVEMESSGVLYSAEPILDPERKV
HCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEE
YLPKITIRKHGIDTNYLFTYDFFQSSDYQRIGKLGAALDGMIEEGGYVQRGERVKEVGSF
ECCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEALDWTISEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRREFIEANALNVANLDV
HHCCCCCHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDI
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
TITIHVDGSVSVKDDGRGIPVAIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSRKLQMTIRRAGKVYEQFYTHGVPDAPIKEIGDATKTGTEIRFWPSEDTFSDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHH
EFHFEILAKRVRELSFLNSGVGIRLIDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKEV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQERDDGITVEVAMQWNDGYQENIFCFTNNIPQRDGGTHLVGFRSALTRNLNNYMEN
HHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKTAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEPALTQYLTTQALDGTVVYPTQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTHITNRMLYHPMITNEMLADEGKMKEWIDAFIHELVEMESSGVLYSAEPILDPERKV
HCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEE
YLPKITIRKHGIDTNYLFTYDFFQSSDYQRIGKLGAALDGMIEEGGYVQRGERVKEVGSF
ECCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEALDWTISEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRREFIEANALNVANLDV
HHCCCCCHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]