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The map label for this gene is dnaN [H]

Identifier: 120596835

GI number: 120596835

Start: 1556

End: 2656

Strand: Direct

Name: dnaN [H]

Synonym: Sputw3181_0002

Alternate gene names: 120596835

Gene position: 1556-2656 (Clockwise)

Preceding gene: 120596834

Following gene: 120596836

Centisome position: 0.03

GC content: 42.42

Gene sequence:

>1101_bases
ATGAAATTCTCAATTGATAGGGATGCCCTGTTAAAGCCGCTTCAATTAGTCTGTGGTGCGGTAGAAAGACGCCATAATTT
GCCCATTTTGTCTAATCTCCTTGTAGAAGTTAGTGGTCACTCTCTTAAATTGACGGGGACAGATTTAGAAGTTGAACTTG
TTGGTCAAGCTGTGGTTCACGGTGATATCGAAGAAGGTCGTACAACAGTTCCTGCTAAGAAGCTGTTAGATATCGTAAAG
TCATTGCCAGAACAAAGCGAATTAAAGGTTGAGCAACAGGATAATAAGTGGTTGCTGCGTTCAGGCCGTAGCCGTTTTTC
CTTAGCGACTTTGCCCGCAGAAGAATATCCCAATGTTGAAGCTTTCCAAGCTGAAATTGAATTTAGTTTAAAGCAAGGCG
TATTGAAGTCGATTATCGATTCGACGCAGTTTTCTATGGCGAACCAAGATGTGCGTTATTACCTCAATGGATTACTCTTT
GAAACTGAAGGTAATATGTTGCGCGCTATTGCGACCGATGGTCATCGTTTAGCCTTAAGTCATCGTGTTATCGATGCCCA
ATTACCTGAAAAACAAGTTATTGTCCCGCGTAAGGGCGTGATGGAAATGGCGCGTTTATTGGAGTCGGACGATTTAGATA
TTGCGATTGCTATCGGTGATAATGCGATTCGTGCGACTACCGCTAACACCGTCTTTACCAGTAAATTAGTGGATGGTCGC
TTCCCTGATTATCGCCGTGTATTACCAAAGGGCGGTGATAAAATTGTGATCGCGAGTCGCAATCATTTGAAACAAGCACT
GACTCGGGCATCGATTTTATCCAACGAAAAATTCCGTGGTGTGCGTATTCAACTCGAAAATGGTTTGCTCAAGATTACCG
CTAACAATCCTGAACAGGAAGAAGCGGAAGAGATCATCGATGTTGACTATAACAATCAATCACTTGAAATTGGTTTTAAC
GTCAGTTATTTACTGGACGTGCTCAATAACCTTAAGTCCGATGATGTGCGTATCACGCTGATTGATGGTAACTCTAGCGC
TTTAATTGAAAACCATTTGGAAGAAGATTCCATGTATGTGGTTATGCCGATGCGTTTGTAG

Upstream 100 bases:

>100_bases
GTAAAATTGCTCAGCTACGGGAAGAGAGCCACGACATAAAAGAAGATTATGCTAATTTGATTAGAACGCTATCTTCTTAA
AAACAAGGAATTTGGACACT

Downstream 100 bases:

>100_bases
TTCACGTCACATACTTGTCCATGTTTACCCGTTTATCTTTGCTATCTACGCCGCGTGTTAGTGGCGTAGATTTCCTCAAA
ATACTCCTGTTAAACCATCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQAVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNMLRAIATDGHRLALSHRVIDAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVDYNNQSLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQAVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNMLRAIATDGHRLALSHRVIDAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVDYNNQSLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQAVVHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLF
ETEGNMLRAIATDGHRLALSHRVIDAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQEEAEEIIDVDYNNQSLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=57.6502732240437, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40938; Mature: 40938

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQAVVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEECCEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH
AFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLFETEGNMLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCEEEEEECCCCEEEEE
HRVIDAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
EEEEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNNQSLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYV
HHHHHEECCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILSNLLVEVSGHSLKLTGTDLEVELVGQAVVH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEECCEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH
AFQAEIEFSLKQGVLKSIIDSTQFSMANQDVRYYLNGLLFETEGNMLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCEEEEEECCCCEEEEE
HRVIDAQLPEKQVIVPRKGVMEMARLLESDDLDIAIAIGDNAIRATTANTVFTSKLVDGR
EEEEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHLKQALTRASILSNEKFRGVRIQLENGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNNQSLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALIENHLEEDSMYV
HHHHHEECCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]