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The map label for this gene is dnaA

Identifier: 120596834

GI number: 120596834

Start: 150

End: 1535

Strand: Direct

Name: dnaA

Synonym: Sputw3181_0001

Alternate gene names: 120596834

Gene position: 150-1535 (Clockwise)

Preceding gene: NA

Following gene: 120596835

Centisome position: 0.0

GC content: 43.36

Gene sequence:

>1386_bases
GTGGCGGTTTCACTTTGGCAACAATGTATCGGACGACTGCAAGATGAGCTTCCTGCTCAGCAGTTTAGTATGTGGATCAG
ACCGTTACAAGCTGAAATGGATGGTGATACATTGGTGCTCTATGCGCCCAACCGCTTCGTGCTCGATTGGGTCAGAGAAA
AGTACATTAATATCATTAATCAGTTTTTTACTGAGCAAATGGGTAGCGACGCACCCAAATTACGCTTCGATATTGGTAGT
CGTCCATCGGCTAAAAAGTTTGAGCCCGCCCCAGTAGCGACTGTGCGTGCACCTAATACTCAAACTAAAGCGACTGTCGG
TACTTATTTTAATACCCAAGCTGAGCCTATTGCTAATGCTAATCATCGTAGCAATATCAATCCAACCTATCAGTTTGATA
ACTTCGTCGAAGGTAAATCAAACCAATTAGGTAAAGCTGCAGCGTTGCAAGTGGCTGAAAATCCTGGTGGTGCCTATAAC
CCATTATTCTTATATGGTGGAACAGGTTTAGGTAAGACTCACTTGTTGCATGCTGTCGGGAACGGGATCATCAAAAATAA
TCCTAATGCCAAAGTGGTTTATATGCATTCTGAGCGTTTTGTCCAAGATATGGTTAAAGCGCTGCAAAATAATGCCATCG
AAGAATTTAAACGTTACTACCGTAGTGTCGATGCACTGTTTATCGATGACATTCAATTCTTTGCCAATAAAGACAGATCT
CAAGAAGAATTCTTCCATACCTTCAATGCCTTGCTTGAAGGCAACCACCAAATTATTTTGACTTCAGATCGTTATCCAAA
AGAGATCGATGGTGTTGAAGACAGATTGAAATCGCGTTTTGGTTGGGGGTTAACCGTGGCGATTGAGCCACCCGAGTTAG
AAACCCGTGTGGCGATTTTGATGCGTAAAGCCCAAGAGAGTGGTATTAACTTGCCCGATGAAGTGGCCTTCTTTATTGCT
AAGCGCTTACGTTCAAACGTGCGTGAGCTAGAAGGTGCGTTAAACCGCGTTATTGCGAACGCTAACTTTACTGGTCGACC
AATCACCATTGATTTTGTTCGTGAAGCACTGCGTGACCTCTTGGCACTTCAAGAAAAATTAGTCACTATAGACAATATTC
AGAAAACCGTAGCTGAATACTACAAGATCAAGATGGCTGATATGTTGTCTAAGCGCCGTTCACGCAGTGTCGCGCGTCCA
AGACAAGTTGCCATGGCGTTATCCAAAGAATTAACTAACCAGAGCTTGCCTGAAATTGGTGATGCTTTTGGTGGTCGCGA
TCACACCACAGTGTTGCATGCCTGTCGTAAAATTGCTCAGCTACGGGAAGAGAGCCACGACATAAAAGAAGATTATGCTA
ATTTGATTAGAACGCTATCTTCTTAA

Upstream 100 bases:

>100_bases
AGAATGATCTTGCTGGGGATAAATATTTTTTAAGGATAGCGATCCTTGAGATCTCGCTATAGAATACACCTCTTTTTGAT
GATCTTTTGGGGATAACTAA

Downstream 100 bases:

>100_bases
AAACAAGGAATTTGGACACTATGAAATTCTCAATTGATAGGGATGCCCTGTTAAAGCCGCTTCAATTAGTCTGTGGTGCG
GTAGAAAGACGCCATAATTT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAVSLWQQCIGRLQDELPAQQFSMWIRPLQAEMDGDTLVLYAPNRFVLDWVREKYINIINQFFTEQMGSDAPKLRFDIGS
RPSAKKFEPAPVATVRAPNTQTKATVGTYFNTQAEPIANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYN
PLFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
QEEFFHTFNALLEGNHQIILTSDRYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIA
KRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARP
RQVAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS

Sequences:

>Translated_461_residues
MAVSLWQQCIGRLQDELPAQQFSMWIRPLQAEMDGDTLVLYAPNRFVLDWVREKYINIINQFFTEQMGSDAPKLRFDIGS
RPSAKKFEPAPVATVRAPNTQTKATVGTYFNTQAEPIANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYN
PLFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
QEEFFHTFNALLEGNHQIILTSDRYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIA
KRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARP
RQVAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
>Mature_460_residues
AVSLWQQCIGRLQDELPAQQFSMWIRPLQAEMDGDTLVLYAPNRFVLDWVREKYINIINQFFTEQMGSDAPKLRFDIGSR
PSAKKFEPAPVATVRAPNTQTKATVGTYFNTQAEPIANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNP
LFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRSQ
EEFFHTFNALLEGNHQIILTSDRYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIAK
RLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPR
QVAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=469, Percent_Identity=79.5309168443497, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=231, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52239; Mature: 52108

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSLWQQCIGRLQDELPAQQFSMWIRPLQAEMDGDTLVLYAPNRFVLDWVREKYINIIN
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
QFFTEQMGSDAPKLRFDIGSRPSAKKFEPAPVATVRAPNTQTKATVGTYFNTQAEPIANA
HHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCC
NHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNPLFLYGGTGLGKTHLLHAVG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHC
NGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
CCCEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QEEFFHTFNALLEGNHQIILTSDRYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAIL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHHH
MRKAQESGINLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPRQVAMALSKELTNQSLPEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHH
DAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVSLWQQCIGRLQDELPAQQFSMWIRPLQAEMDGDTLVLYAPNRFVLDWVREKYINIIN
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
QFFTEQMGSDAPKLRFDIGSRPSAKKFEPAPVATVRAPNTQTKATVGTYFNTQAEPIANA
HHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCC
NHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNPLFLYGGTGLGKTHLLHAVG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHC
NGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
CCCEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QEEFFHTFNALLEGNHQIILTSDRYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAIL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHHH
MRKAQESGINLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPRQVAMALSKELTNQSLPEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHH
DAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA