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The map label for this gene is rsuA [H]

Identifier: 119947129

GI number: 119947129

Start: 4345568

End: 4346260

Strand: Direct

Name: rsuA [H]

Synonym: Ping_3527

Alternate gene names: 119947129

Gene position: 4345568-4346260 (Clockwise)

Preceding gene: 119947128

Following gene: 119947130

Centisome position: 95.31

GC content: 39.68

Gene sequence:

>693_bases
ATGAGATTAGATAAATACATTTGTGAGTCTACCACTATGACCAGAAGCCAGGCTAAAAAGGTCATTATCCAGGGACGTAT
AACAAGCGACGGCACTGTCATGCGTAATACGGGTTATAAAGTATTAGAGAATACACCAATCTGTTTTGATGGTAACTTAA
TTAATGTCCGTGGCACACGTTATATTATGCTTAACAAACCGAAAGATTTTATCTGCTCAACTGTTGATGAACAGTTCCCC
AGTATTATTAATTTAATAAAAGTTGATAAACCTGATCAGCTCTGTATTGCAGGTCGACTCGACGCCGATACAACGGGTTT
AGCACTGATTACCGATGATGGGCAATGGTCTCACAAAATCACCAGCCCGCGCAGAAATTGCAGTAAACGCTATCGCGCCA
CACTTGCCCAACCCGTTGAACAAAATGCAGTTACTTTGTTTAAAGAAGGAATACAACTGAAAAGTGAAAATGTCCCCTGC
TTACCTGCAACATTAGAAATTTTAACGGACAATGAAGTTTTACTGACCATTCATGAAGGTAAATACCATCAGGTAAAACG
CATGTTTGCCGCTATTGGTAATCTGGTAGTCGAACTGCACCGCGAGCAGATTGGTGAAATTAAGTTAGATACAGATCTTA
AATTAGGCGAATGGCGTTATTTAACCACCGATGAAGTTAACTCGGTTAAATAA

Upstream 100 bases:

>100_bases
TTTGCAGAAAAAATAATTTAACAGGCAAAAAACAAGCCCAATATATTCTAAAAGAAACCATCAAACAGTTAACCACTAAC
GTTACCAAAAGAGGAAATCA

Downstream 100 bases:

>100_bases
ACGAATAGCATTCTTTGATTAGGAACGATAATAGTATTGTCGTTCCTGCAACGAAAACTAATCAATCGCTAGGAAAAAAG
ATGAACTATCAAGCAGCAGT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRLDKYICESTTMTRSQAKKVIIQGRITSDGTVMRNTGYKVLENTPICFDGNLINVRGTRYIMLNKPKDFICSTVDEQFP
SIINLIKVDKPDQLCIAGRLDADTTGLALITDDGQWSHKITSPRRNCSKRYRATLAQPVEQNAVTLFKEGIQLKSENVPC
LPATLEILTDNEVLLTIHEGKYHQVKRMFAAIGNLVVELHREQIGEIKLDTDLKLGEWRYLTTDEVNSVK

Sequences:

>Translated_230_residues
MRLDKYICESTTMTRSQAKKVIIQGRITSDGTVMRNTGYKVLENTPICFDGNLINVRGTRYIMLNKPKDFICSTVDEQFP
SIINLIKVDKPDQLCIAGRLDADTTGLALITDDGQWSHKITSPRRNCSKRYRATLAQPVEQNAVTLFKEGIQLKSENVPC
LPATLEILTDNEVLLTIHEGKYHQVKRMFAAIGNLVVELHREQIGEIKLDTDLKLGEWRYLTTDEVNSVK
>Mature_230_residues
MRLDKYICESTTMTRSQAKKVIIQGRITSDGTVMRNTGYKVLENTPICFDGNLINVRGTRYIMLNKPKDFICSTVDEQFP
SIINLIKVDKPDQLCIAGRLDADTTGLALITDDGQWSHKITSPRRNCSKRYRATLAQPVEQNAVTLFKEGIQLKSENVPC
LPATLEILTDNEVLLTIHEGKYHQVKRMFAAIGNLVVELHREQIGEIKLDTDLKLGEWRYLTTDEVNSVK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=50.4347826086956, Blast_Score=237, Evalue=4e-64,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=30.2127659574468, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26055; Mature: 26055

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKYICESTTMTRSQAKKVIIQGRITSDGTVMRNTGYKVLENTPICFDGNLINVRGTR
CCCHHHHHCCCHHCHHHHCEEEEEEEECCCCEEEECCCCEEECCCCEEEECCEEEECCEE
YIMLNKPKDFICSTVDEQFPSIINLIKVDKPDQLCIAGRLDADTTGLALITDDGQWSHKI
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCC
TSPRRNCSKRYRATLAQPVEQNAVTLFKEGIQLKSENVPCLPATLEILTDNEVLLTIHEG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCEEEEEEECC
KYHQVKRMFAAIGNLVVELHREQIGEIKLDTDLKLGEWRYLTTDEVNSVK
CHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEECCEEEECCCCCCCCC
>Mature Secondary Structure
MRLDKYICESTTMTRSQAKKVIIQGRITSDGTVMRNTGYKVLENTPICFDGNLINVRGTR
CCCHHHHHCCCHHCHHHHCEEEEEEEECCCCEEEECCCCEEECCCCEEEECCEEEECCEE
YIMLNKPKDFICSTVDEQFPSIINLIKVDKPDQLCIAGRLDADTTGLALITDDGQWSHKI
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCC
TSPRRNCSKRYRATLAQPVEQNAVTLFKEGIQLKSENVPCLPATLEILTDNEVLLTIHEG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCEEEEEEECC
KYHQVKRMFAAIGNLVVELHREQIGEIKLDTDLKLGEWRYLTTDEVNSVK
CHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]