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The map label for this gene is glnA [H]

Identifier: 119947086

GI number: 119947086

Start: 4310715

End: 4312133

Strand: Direct

Name: glnA [H]

Synonym: Ping_3484

Alternate gene names: 119947086

Gene position: 4310715-4312133 (Clockwise)

Preceding gene: 119947078

Following gene: 119947087

Centisome position: 94.54

GC content: 44.47

Gene sequence:

>1419_bases
ATGTCTACAAAAACAGTACAAGATGTTCTTGATTTTATTAAAGAGCAAGAGGTAAAATTCGTAGATTTACGCTTTACCGA
TACACACGGTAAAGAGCAACACGTCTCTATTCCGCATCACCAAGCAAACGAAGACTTTTTCGAAGACGGTAAAATGTTTG
ATGGCTCTTCAATTGCAGGATGGAAAGGCATTCAAGATTCGGACATGGTATTAATGCCGGATCCAACCACTGCTGTTCTT
GACCCCTTTACGGATGATGCAACGCTTAACCTGCGTTGTGATGTGTTAGAGCCTAAAACAATGCTTGGTTACGATCGTGA
CCCGCGATCTGTTGCTAAACGCGCCGAAGCATACCTGCGTTCAACGGGCATTGCTGACACTATTTTATTTGGTCCAGAGC
CCGAGTTTTTCCTATTTGACGATGTTAAATACGGTAATACTATGCAAGGTGCATTCTTTTCCATTGATGCTAAAGAAGCG
GCGTGGAACTCAGGTACCTCCTATGAAGAAGGCAATACAGGCCACCGTCCTGGTATCAAAGGTGGTTATTTCCCTGTCGC
CCCCGTAGATTCCGCACAAAACATCCGTAGTGCTATGTGTCTTATCATGGAAGAGATGGGCCTTATTGTTGAAGCACATC
ATCATGAAGTCGCAACTGCCGGCCAAAATGAAATCGCTTGTCGTTTTAATACTATGGTTAAAAAAGCCGATGAACTACAA
ATTTACAAATACGTAGTACATAACGTTGCTCATGCCTACGGTCAAACCGCAACCTTCATGCCTAAACCGCTTGTTGGTGA
TAACGGCTCAGGTAATCACTGTCACCAGTCCCTAGGCAAAGACGGTGTTAACCTGTTCTCCGGCGACCAGTACGGCGGGC
TTTCTCAGCTAGCGCTTTATTATATTGGTGGCCTTATCAAACATGCTAAAGCACTTAATGCCTTTACTAACGCGTCAACT
AACTCATATAAACGTCTAGTGCCACATTTTGAAGCACCGGTTATGTTAGCGTACTCTGCCGGTAATCGCAGCGCATCTAT
CCGTATTCCAATCGTACCGTCACCAAAAGCGGCACGTGTTGAACTACGTTTCCCGGACCCAACGATGAACCCTTATTTAG
GTTTTTCTGCGATGCTAATGGCGGGTCTTGACGGTATTAAAAACAAGATCCACCCTGGCGAGCCTCTTGATAAAGATTTA
TACGATCTGCCTAAAGAAGAAGCGGATGCAATACCACAAGTTGCTGTTTCTTTTGAAGAAGCACTTGCTGAACTCGATGC
TGACCGTGACTTTTTAACCGCAGGAGATGTTTTCTCGAATGACTCGATTGATGCCTACATCGCCCTTAAAACAGCTGAAT
GTGAAGCTGTTTCTCGTGCAACGCATCCAAAAGAGTTCGAATTATACTACAGCGTATAA

Upstream 100 bases:

>100_bases
TAATGGATAGCAGTCAGGTCGGCACAATTTTAGCCGTTTGAGTAATTAAATAAATTTGTTATGGTTGTCCACCACTTAAA
TGTCAATTGAGGTTCTAAAC

Downstream 100 bases:

>100_bases
GCAAAGAACTTAGTTAGGTTATGAACACCTGCTCACACTAATTCCGCTTAATTAACGGAATTGTCATCAGCTCAAAGGAT
CAACTAGTTTGATCCTTTTA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTKTVQDVLDFIKEQEVKFVDLRFTDTHGKEQHVSIPHHQANEDFFEDGKMFDGSSIAGWKGIQDSDMVLMPDPTTAVL
DPFTDDATLNLRCDVLEPKTMLGYDRDPRSVAKRAEAYLRSTGIADTILFGPEPEFFLFDDVKYGNTMQGAFFSIDAKEA
AWNSGTSYEEGNTGHRPGIKGGYFPVAPVDSAQNIRSAMCLIMEEMGLIVEAHHHEVATAGQNEIACRFNTMVKKADELQ
IYKYVVHNVAHAYGQTATFMPKPLVGDNGSGNHCHQSLGKDGVNLFSGDQYGGLSQLALYYIGGLIKHAKALNAFTNAST
NSYKRLVPHFEAPVMLAYSAGNRSASIRIPIVPSPKAARVELRFPDPTMNPYLGFSAMLMAGLDGIKNKIHPGEPLDKDL
YDLPKEEADAIPQVAVSFEEALAELDADRDFLTAGDVFSNDSIDAYIALKTAECEAVSRATHPKEFELYYSV

Sequences:

>Translated_472_residues
MSTKTVQDVLDFIKEQEVKFVDLRFTDTHGKEQHVSIPHHQANEDFFEDGKMFDGSSIAGWKGIQDSDMVLMPDPTTAVL
DPFTDDATLNLRCDVLEPKTMLGYDRDPRSVAKRAEAYLRSTGIADTILFGPEPEFFLFDDVKYGNTMQGAFFSIDAKEA
AWNSGTSYEEGNTGHRPGIKGGYFPVAPVDSAQNIRSAMCLIMEEMGLIVEAHHHEVATAGQNEIACRFNTMVKKADELQ
IYKYVVHNVAHAYGQTATFMPKPLVGDNGSGNHCHQSLGKDGVNLFSGDQYGGLSQLALYYIGGLIKHAKALNAFTNAST
NSYKRLVPHFEAPVMLAYSAGNRSASIRIPIVPSPKAARVELRFPDPTMNPYLGFSAMLMAGLDGIKNKIHPGEPLDKDL
YDLPKEEADAIPQVAVSFEEALAELDADRDFLTAGDVFSNDSIDAYIALKTAECEAVSRATHPKEFELYYSV
>Mature_471_residues
STKTVQDVLDFIKEQEVKFVDLRFTDTHGKEQHVSIPHHQANEDFFEDGKMFDGSSIAGWKGIQDSDMVLMPDPTTAVLD
PFTDDATLNLRCDVLEPKTMLGYDRDPRSVAKRAEAYLRSTGIADTILFGPEPEFFLFDDVKYGNTMQGAFFSIDAKEAA
WNSGTSYEEGNTGHRPGIKGGYFPVAPVDSAQNIRSAMCLIMEEMGLIVEAHHHEVATAGQNEIACRFNTMVKKADELQI
YKYVVHNVAHAYGQTATFMPKPLVGDNGSGNHCHQSLGKDGVNLFSGDQYGGLSQLALYYIGGLIKHAKALNAFTNASTN
SYKRLVPHFEAPVMLAYSAGNRSASIRIPIVPSPKAARVELRFPDPTMNPYLGFSAMLMAGLDGIKNKIHPGEPLDKDLY
DLPKEEADAIPQVAVSFEEALAELDADRDFLTAGDVFSNDSIDAYIALKTAECEAVSRATHPKEFELYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=73.2905982905983, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51960; Mature: 51829

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKTVQDVLDFIKEQEVKFVDLRFTDTHGKEQHVSIPHHQANEDFFEDGKMFDGSSIAG
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCC
WKGIQDSDMVLMPDPTTAVLDPFTDDATLNLRCDVLEPKTMLGYDRDPRSVAKRAEAYLR
CCCCCCCCEEEECCCCHHEECCCCCCCEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHH
STGIADTILFGPEPEFFLFDDVKYGNTMQGAFFSIDAKEAAWNSGTSYEEGNTGHRPGIK
HCCCCEEEEECCCCCEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCC
GGYFPVAPVDSAQNIRSAMCLIMEEMGLIVEAHHHEVATAGQNEIACRFNTMVKKADELQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEHHHHHHHHHHHH
IYKYVVHNVAHAYGQTATFMPKPLVGDNGSGNHCHQSLGKDGVNLFSGDQYGGLSQLALY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCCCCCHHHHHHH
YIGGLIKHAKALNAFTNASTNSYKRLVPHFEAPVMLAYSAGNRSASIRIPIVPSPKAARV
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEE
ELRFPDPTMNPYLGFSAMLMAGLDGIKNKIHPGEPLDKDLYDLPKEEADAIPQVAVSFEE
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
ALAELDADRDFLTAGDVFSNDSIDAYIALKTAECEAVSRATHPKEFELYYSV
HHHHHCCCCCEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
STKTVQDVLDFIKEQEVKFVDLRFTDTHGKEQHVSIPHHQANEDFFEDGKMFDGSSIAG
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCC
WKGIQDSDMVLMPDPTTAVLDPFTDDATLNLRCDVLEPKTMLGYDRDPRSVAKRAEAYLR
CCCCCCCCEEEECCCCHHEECCCCCCCEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHH
STGIADTILFGPEPEFFLFDDVKYGNTMQGAFFSIDAKEAAWNSGTSYEEGNTGHRPGIK
HCCCCEEEEECCCCCEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCC
GGYFPVAPVDSAQNIRSAMCLIMEEMGLIVEAHHHEVATAGQNEIACRFNTMVKKADELQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEHHHHHHHHHHHH
IYKYVVHNVAHAYGQTATFMPKPLVGDNGSGNHCHQSLGKDGVNLFSGDQYGGLSQLALY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCCCCCHHHHHHH
YIGGLIKHAKALNAFTNASTNSYKRLVPHFEAPVMLAYSAGNRSASIRIPIVPSPKAARV
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCEE
ELRFPDPTMNPYLGFSAMLMAGLDGIKNKIHPGEPLDKDLYDLPKEEADAIPQVAVSFEE
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
ALAELDADRDFLTAGDVFSNDSIDAYIALKTAECEAVSRATHPKEFELYYSV
HHHHHCCCCCEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]