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The map label for this gene is rlmB [H]

Identifier: 119947021

GI number: 119947021

Start: 4225117

End: 4225857

Strand: Direct

Name: rlmB [H]

Synonym: Ping_3418

Alternate gene names: 119947021

Gene position: 4225117-4225857 (Clockwise)

Preceding gene: 119947020

Following gene: 119947022

Centisome position: 92.66

GC content: 44.67

Gene sequence:

>741_bases
ATGAGCAAAAGTGAATTACTTTATGGCATCCATTCAGTTGATGCCTTATTAGAGAAACAACCAGAACGTTTTATTGAAGT
TTATGCATTAAAAGGACGTGAGGACGAACGCCTTAATAATATTATTGCTAAATTAAATGAATGGGGTATTTCCGTCCAAT
TAATGAACCGTAAAGCCATGGATGATAAGTCGGAAGGAGAGCAACACCAGGGTATTATCGCCCGCGTTAAAACGGCCAAG
GTCTTAAACGATAATGACTTAGACAGTTTATTGGAGAGTTTAACTGCACCGCCTTTTTTACTTATTTTAGACGGTGTAAC
GGATCCGCATAATCTAGGTGCATGTCTGCGTAGTGCAGATGCAGCCGGTGTGCATGCAGTGATTATCCCTAAAGATAATT
CAGCAAGCTTAACCCCCGTAGTGCGTAAAGTCGCCTGTGGAGCAGCAGAATCAGTGCCTTTAATTCATGTCACTAATCTG
GCTCGAGCGATGCGTTCATTACAGGACAAAGGTGTCTGGATTTATGGTACAGCGGGAGAAGCCACACAAAGCCTCTATGA
CTGTAAACTAGAAGGCGGCATGGCACTTGCGATGGGCGCGGAAGGTAAAGGATTACGCCGTTTAACACGTGAACATTGTG
ACCAGCTGATTAAACTGCCAATGGCGGGAGCCGTTTCCAGCTTAAATGTCTCGGTAGCAACCGGTGTCTGCTTGTTTGAA
GTGGTTCGTCAGCGCGGATAA

Upstream 100 bases:

>100_bases
GGAGCTACGAGCGGCGAGCTAGGAGATACAAGCTTTCTAGCTTTCAGGCGTTCGAGCTGCGAGTCTTGAGCTATTATCAA
CAAATAGAATTAGAGAAAAT

Downstream 100 bases:

>100_bases
CAAAATTTTCGGCTATCAGCTATCAGCTATCAGCTATCGGCTGACGGCTGATAGCCGATAGCGGCAAGGCTTCGAGCTTT
GACCTAGTGTCCTCTTTCAA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSKSELLYGIHSVDALLEKQPERFIEVYALKGREDERLNNIIAKLNEWGISVQLMNRKAMDDKSEGEQHQGIIARVKTAK
VLNDNDLDSLLESLTAPPFLLILDGVTDPHNLGACLRSADAAGVHAVIIPKDNSASLTPVVRKVACGAAESVPLIHVTNL
ARAMRSLQDKGVWIYGTAGEATQSLYDCKLEGGMALAMGAEGKGLRRLTREHCDQLIKLPMAGAVSSLNVSVATGVCLFE
VVRQRG

Sequences:

>Translated_246_residues
MSKSELLYGIHSVDALLEKQPERFIEVYALKGREDERLNNIIAKLNEWGISVQLMNRKAMDDKSEGEQHQGIIARVKTAK
VLNDNDLDSLLESLTAPPFLLILDGVTDPHNLGACLRSADAAGVHAVIIPKDNSASLTPVVRKVACGAAESVPLIHVTNL
ARAMRSLQDKGVWIYGTAGEATQSLYDCKLEGGMALAMGAEGKGLRRLTREHCDQLIKLPMAGAVSSLNVSVATGVCLFE
VVRQRG
>Mature_245_residues
SKSELLYGIHSVDALLEKQPERFIEVYALKGREDERLNNIIAKLNEWGISVQLMNRKAMDDKSEGEQHQGIIARVKTAKV
LNDNDLDSLLESLTAPPFLLILDGVTDPHNLGACLRSADAAGVHAVIIPKDNSASLTPVVRKVACGAAESVPLIHVTNLA
RAMRSLQDKGVWIYGTAGEATQSLYDCKLEGGMALAMGAEGKGLRRLTREHCDQLIKLPMAGAVSSLNVSVATGVCLFEV
VRQRG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=232, Percent_Identity=31.0344827586207, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=68.1818181818182, Blast_Score=342, Evalue=1e-95,
Organism=Escherichia coli, GI1788935, Length=245, Percent_Identity=27.3469387755102, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=31.6129032258064, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=260, Percent_Identity=28.4615384615385, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324775, Length=166, Percent_Identity=33.1325301204819, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26597; Mature: 26466

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSELLYGIHSVDALLEKQPERFIEVYALKGREDERLNNIIAKLNEWGISVQLMNRKAM
CCCCHHHHHHHHHHHHHHHCHHHHHHHHEECCCCHHHHHHHHHHHHHCCCEEEEECCCCC
DDKSEGEQHQGIIARVKTAKVLNDNDLDSLLESLTAPPFLLILDGVTDPHNLGACLRSAD
CCCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
AAGVHAVIIPKDNSASLTPVVRKVACGAAESVPLIHVTNLARAMRSLQDKGVWIYGTAGE
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCH
ATQSLYDCKLEGGMALAMGAEGKGLRRLTREHCDQLIKLPMAGAVSSLNVSVATGVCLFE
HHHHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHH
VVRQRG
HHHHCC
>Mature Secondary Structure 
SKSELLYGIHSVDALLEKQPERFIEVYALKGREDERLNNIIAKLNEWGISVQLMNRKAM
CCCHHHHHHHHHHHHHHHCHHHHHHHHEECCCCHHHHHHHHHHHHHCCCEEEEECCCCC
DDKSEGEQHQGIIARVKTAKVLNDNDLDSLLESLTAPPFLLILDGVTDPHNLGACLRSAD
CCCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
AAGVHAVIIPKDNSASLTPVVRKVACGAAESVPLIHVTNLARAMRSLQDKGVWIYGTAGE
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCH
ATQSLYDCKLEGGMALAMGAEGKGLRRLTREHCDQLIKLPMAGAVSSLNVSVATGVCLFE
HHHHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHH
VVRQRG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]