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The map label for this gene is phoA [H]

Identifier: 119947012

GI number: 119947012

Start: 4210910

End: 4212568

Strand: Direct

Name: phoA [H]

Synonym: Ping_3409

Alternate gene names: 119947012

Gene position: 4210910-4212568 (Clockwise)

Preceding gene: 119947011

Following gene: 119947013

Centisome position: 92.35

GC content: 45.45

Gene sequence:

>1659_bases
ATGAAAACTAACAATAACATAAGTCTATTTAAACCGAGTCTAATCGCTGTTGCCGTACTCGCGTTAAGCGCGTGTAATAA
TTTGCAGGAAACACAGCAATTATCAAAGCAACAAATCACTGAAAACAGCAGCTCTGCATGGTATATAGATGGCCAGCAGC
AAGTTAAAAATGCGCAAGCACAGATCGATAATATCGTTAAAACACGCGGACAAGCCAAAAATATCATTTTATTTGTTGGT
GATGGTATGGGCGTTTCAACGCTAACGGCTGCGCGGATTTTAGAAGGCCAAAACAAAGGAATGCTGGGAGAGGAAAACCG
TCTTAGCTTTGAAAACATGCCTTTTACAGGATTTGCCAAAACCTACAATGTCGATGCACAAACACCCGATTCAGCCGGGA
CGATGACCGCAATTATCTCTGGCGTTAAGACCGATAAAGGCGTTATTGGTGTTGGTGAACAAGTCATTCGTGGAGAATGT
ACAAGTGCAGCAGGCAACGAACTGGTCAGCTCACTGGAACTGGCTGAAATCGCCGGTAAATCAACAGGTATCATCTCCAC
TGCCCGAATCACTCATGCTACTCCAGCTGCCACTTATGCTAAATCTGCAGATCGCAATTGGGAAGATATTTCCGATATGC
CGGCAGCCGCTGTCACAGCTGGCTGTAAAGATATCGCAGATCAACTGGTTAACTTTGAAAGTAACCTTGAAGCACGCTAT
AGAGGGATCGATGTTAATGGTCTTGAAGTAGTCATGGGCGGTGGTCGCAGACATTTCTTACCCAAAGATGCCAGCTTTAA
TAGCCCCGATGCAGTCAGTGCAACAGAAGGTGACCGCAGCGATGGACGGGATCTAACGGCAGAATGGCAAACAGCCTATA
GTAATGGCCAATATATCTTTGACCAAGCGGGCTTTGATGCCATAGACACTGAAAATACTGAGCGTGTTTTTGGCCTGTTT
AATGAATCACATATGCACTACGAAGCTGATAGAAATAACGATATCTCCGGCGAACCTTCGCTGTCACAAATGACCGAAAA
AGCGATGGCGATTCTTGACAATAATACTCAGGGCTACTTTTTAATGGTCGAAGCAGGCCGTATTGACCATGCACATCATG
CCGGCAGTGCTTATAACGCGTTAACCGATACGATTGAATTATCCAACGCAGTTAAACTTGCCATGCAAAACAGCAACCCA
GCAGAGACCTTAATTATTGTCACCGCCGACCATGGCCATGTGTTTACCATTGCCGGATACCCTAAGCGTGGCAATCCTAT
TTTAGGTAAAGTGGTTAGCATAGGAAAAACACAGCCAGCTTTAGCGGCAGATGATCTTCCTTACACCACAGTAGGTTACA
CAAACGGACTCGGCTTTAGAGATTTAGGCGTAGGGGTTACAAATGCTGATGCGGCCTATGCTGATGCTCCGGTAACAGGA
CGAGTGGATTTAAGTAATATTGATACGAGCAACAGCGGATTCCACCAGGAAGCACTGGTTCCATTATCTTCTGAAACACA
TTCAGGTGAAGATGTCGCGGTTTTTGCATCGGGTCCTGGCGCGCACCTGGTCAGCGGTAACTACGAACAAAGTCTTTTAT
TCCATGTTATGGATTTTGCCGGAGACTTAGTCACGAAAGCAGATCAGAAACTACCGTAA

Upstream 100 bases:

>100_bases
TCCCGGTGCGCAGTTGGCCCAGGGAAATATTGAACAGAACGTGATTTATCACATTATCGAAAACGCATTAGATTTAGTTG
GCAAGGAATAAGGTAACGAT

Downstream 100 bases:

>100_bases
GACAATCTGAGCAGAGCATATTTTTATGCTTCTCATTATCAAAGGGGCTTTTGCCCCTTCATTATTATTTCAGGATTATA
TTTTGAAGAGACTTACGGTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKTNNNISLFKPSLIAVAVLALSACNNLQETQQLSKQQITENSSSAWYIDGQQQVKNAQAQIDNIVKTRGQAKNIILFVG
DGMGVSTLTAARILEGQNKGMLGEENRLSFENMPFTGFAKTYNVDAQTPDSAGTMTAIISGVKTDKGVIGVGEQVIRGEC
TSAAGNELVSSLELAEIAGKSTGIISTARITHATPAATYAKSADRNWEDISDMPAAAVTAGCKDIADQLVNFESNLEARY
RGIDVNGLEVVMGGGRRHFLPKDASFNSPDAVSATEGDRSDGRDLTAEWQTAYSNGQYIFDQAGFDAIDTENTERVFGLF
NESHMHYEADRNNDISGEPSLSQMTEKAMAILDNNTQGYFLMVEAGRIDHAHHAGSAYNALTDTIELSNAVKLAMQNSNP
AETLIIVTADHGHVFTIAGYPKRGNPILGKVVSIGKTQPALAADDLPYTTVGYTNGLGFRDLGVGVTNADAAYADAPVTG
RVDLSNIDTSNSGFHQEALVPLSSETHSGEDVAVFASGPGAHLVSGNYEQSLLFHVMDFAGDLVTKADQKLP

Sequences:

>Translated_552_residues
MKTNNNISLFKPSLIAVAVLALSACNNLQETQQLSKQQITENSSSAWYIDGQQQVKNAQAQIDNIVKTRGQAKNIILFVG
DGMGVSTLTAARILEGQNKGMLGEENRLSFENMPFTGFAKTYNVDAQTPDSAGTMTAIISGVKTDKGVIGVGEQVIRGEC
TSAAGNELVSSLELAEIAGKSTGIISTARITHATPAATYAKSADRNWEDISDMPAAAVTAGCKDIADQLVNFESNLEARY
RGIDVNGLEVVMGGGRRHFLPKDASFNSPDAVSATEGDRSDGRDLTAEWQTAYSNGQYIFDQAGFDAIDTENTERVFGLF
NESHMHYEADRNNDISGEPSLSQMTEKAMAILDNNTQGYFLMVEAGRIDHAHHAGSAYNALTDTIELSNAVKLAMQNSNP
AETLIIVTADHGHVFTIAGYPKRGNPILGKVVSIGKTQPALAADDLPYTTVGYTNGLGFRDLGVGVTNADAAYADAPVTG
RVDLSNIDTSNSGFHQEALVPLSSETHSGEDVAVFASGPGAHLVSGNYEQSLLFHVMDFAGDLVTKADQKLP
>Mature_552_residues
MKTNNNISLFKPSLIAVAVLALSACNNLQETQQLSKQQITENSSSAWYIDGQQQVKNAQAQIDNIVKTRGQAKNIILFVG
DGMGVSTLTAARILEGQNKGMLGEENRLSFENMPFTGFAKTYNVDAQTPDSAGTMTAIISGVKTDKGVIGVGEQVIRGEC
TSAAGNELVSSLELAEIAGKSTGIISTARITHATPAATYAKSADRNWEDISDMPAAAVTAGCKDIADQLVNFESNLEARY
RGIDVNGLEVVMGGGRRHFLPKDASFNSPDAVSATEGDRSDGRDLTAEWQTAYSNGQYIFDQAGFDAIDTENTERVFGLF
NESHMHYEADRNNDISGEPSLSQMTEKAMAILDNNTQGYFLMVEAGRIDHAHHAGSAYNALTDTIELSNAVKLAMQNSNP
AETLIIVTADHGHVFTIAGYPKRGNPILGKVVSIGKTQPALAADDLPYTTVGYTNGLGFRDLGVGVTNADAAYADAPVTG
RVDLSNIDTSNSGFHQEALVPLSSETHSGEDVAVFASGPGAHLVSGNYEQSLLFHVMDFAGDLVTKADQKLP

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=528, Percent_Identity=39.9621212121212, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI294660770, Length=467, Percent_Identity=41.541755888651, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI157266292, Length=477, Percent_Identity=41.5094339622642, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI157266296, Length=513, Percent_Identity=39.1812865497076, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI94721246, Length=481, Percent_Identity=40.7484407484407, Blast_Score=352, Evalue=7e-97,
Organism=Homo sapiens, GI294660772, Length=435, Percent_Identity=39.0804597701149, Blast_Score=319, Evalue=4e-87,
Organism=Escherichia coli, GI48994877, Length=382, Percent_Identity=31.151832460733, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320689, Length=354, Percent_Identity=31.9209039548023, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355149, Length=555, Percent_Identity=38.7387387387387, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI21355151, Length=521, Percent_Identity=41.0748560460653, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI21358067, Length=538, Percent_Identity=39.2193308550186, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI21355981, Length=506, Percent_Identity=39.7233201581028, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI19921912, Length=502, Percent_Identity=39.8406374501992, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24651554, Length=541, Percent_Identity=37.8927911275416, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI85725200, Length=491, Percent_Identity=37.8818737270876, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI85815833, Length=491, Percent_Identity=37.8818737270876, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24657835, Length=548, Percent_Identity=35.2189781021898, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24657842, Length=534, Percent_Identity=37.4531835205993, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24585213, Length=550, Percent_Identity=35.6363636363636, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24651556, Length=475, Percent_Identity=39.3684210526316, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI18859923, Length=535, Percent_Identity=33.6448598130841, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24657827, Length=511, Percent_Identity=35.6164383561644, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24645593, Length=201, Percent_Identity=31.8407960199005, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 58785; Mature: 58785

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNNNISLFKPSLIAVAVLALSACNNLQETQQLSKQQITENSSSAWYIDGQQQVKNAQA
CCCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
QIDNIVKTRGQAKNIILFVGDGMGVSTLTAARILEGQNKGMLGEENRLSFENMPFTGFAK
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
TYNVDAQTPDSAGTMTAIISGVKTDKGVIGVGEQVIRGECTSAAGNELVSSLELAEIAGK
EEECCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHCCC
STGIISTARITHATPAATYAKSADRNWEDISDMPAAAVTAGCKDIADQLVNFESNLEARY
CCCEEEEEEEECCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
RGIDVNGLEVVMGGGRRHFLPKDASFNSPDAVSATEGDRSDGRDLTAEWQTAYSNGQYIF
EECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEE
DQAGFDAIDTENTERVFGLFNESHMHYEADRNNDISGEPSLSQMTEKAMAILDNNTQGYF
ECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCEE
LMVEAGRIDHAHHAGSAYNALTDTIELSNAVKLAMQNSNPAETLIIVTADHGHVFTIAGY
EEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCEEEEECC
PKRGNPILGKVVSIGKTQPALAADDLPYTTVGYTNGLGFRDLGVGVTNADAAYADAPVTG
CCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCC
RVDLSNIDTSNSGFHQEALVPLSSETHSGEDVAVFASGPGAHLVSGNYEQSLLFHVMDFA
EEECCCCCCCCCCCCHHEEEECCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHH
GDLVTKADQKLP
HHHHHHHHCCCC
>Mature Secondary Structure
MKTNNNISLFKPSLIAVAVLALSACNNLQETQQLSKQQITENSSSAWYIDGQQQVKNAQA
CCCCCCCEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
QIDNIVKTRGQAKNIILFVGDGMGVSTLTAARILEGQNKGMLGEENRLSFENMPFTGFAK
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
TYNVDAQTPDSAGTMTAIISGVKTDKGVIGVGEQVIRGECTSAAGNELVSSLELAEIAGK
EEECCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHCCC
STGIISTARITHATPAATYAKSADRNWEDISDMPAAAVTAGCKDIADQLVNFESNLEARY
CCCEEEEEEEECCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
RGIDVNGLEVVMGGGRRHFLPKDASFNSPDAVSATEGDRSDGRDLTAEWQTAYSNGQYIF
EECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEE
DQAGFDAIDTENTERVFGLFNESHMHYEADRNNDISGEPSLSQMTEKAMAILDNNTQGYF
ECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCEE
LMVEAGRIDHAHHAGSAYNALTDTIELSNAVKLAMQNSNPAETLIIVTADHGHVFTIAGY
EEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCEEEEECC
PKRGNPILGKVVSIGKTQPALAADDLPYTTVGYTNGLGFRDLGVGVTNADAAYADAPVTG
CCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCC
RVDLSNIDTSNSGFHQEALVPLSSETHSGEDVAVFASGPGAHLVSGNYEQSLLFHVMDFA
EEECCCCCCCCCCCCHHEEEECCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHH
GDLVTKADQKLP
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]