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The map label for this gene is phoA [H]

Identifier: 119947011

GI number: 119947011

Start: 4209317

End: 4210900

Strand: Direct

Name: phoA [H]

Synonym: Ping_3408

Alternate gene names: 119947011

Gene position: 4209317-4210900 (Clockwise)

Preceding gene: 119947010

Following gene: 119947012

Centisome position: 92.32

GC content: 46.09

Gene sequence:

>1584_bases
ATGGATGTCCGTAAAAGCTTAGTGTCGACCTTAGTGACCTTAGCAATCAGCTCGAGCGTTTCAGCTGGAGTAGTACCAGA
GAATCAAAAAAACACTTGGTTTCAAAGTGCTAATAGTGAAATAACCGAAAAACTGCAGCAGACTTACCAAAAGCGCGCAA
AGAACGTCATTCTCTTTGTCGGTGACGGAATGGGTATCTCCACCTTAACAGCTGCACGTATTCTCGAAGGTCAATTATCA
GGTAACCCCGGTGAAGAGCATCAACTCAGTTTTGAAACATTTCCCTATTCAGCATTAGTTAAAACCTACAATGTCGATGC
GCAGACGCCTGATTCCGCCGGCACTATGACCGCTATGATGTCAGGTGTTAAGAGCGATGCAGGCGTGATTGGTGTTGGCG
AAAAGGTACAGCGCGGTGACTGCTCTACTATGGACAATAACGAGCTGGTAAGCATTGTCGACCTGGCCGAAATAAAAGGC
TTGTCGACAGGCGTTGTCACTACCGCACGTATCACCCACGCGACCCCGGCTGCAACTTACGCCAAATCGGTCGAACGTAA
CTGGGAAGATATCTCAGATATGCCCGATGACGCGATCGCAGGAGGTTGTAAAGATATAGCCGATCAATTAGTTAACTATC
AAAGCAATCTGGAAACCCGTTTTCCAGGTCTCAATGTTGATGGTATTGAAGTGCTCTTTGGCGGTGGTCGACGTCATTTT
TTACCTAAAGACGCAGCCTTTAACAGCTTGGACGCAAGCAGTGAAAAAGAGGGAGATCGCACCGACGGGCGTGATTTAAC
CAGTGAATGGCAAAATATTTATAAAGACGGTACCTATGTCTATGAGCAAACCGGTTTTGATGCTATCGACACGGAAAGCA
GCAGCCATGTTCTCGGTTTGTTTAACGAATCACATATGCAGTATGCAGCGGATCGGACTAATGATATTGCCGGAGAGCCT
TCTCTTTCACAAATGACCGGTAAAGCAATCAATATCCTTGATAATAACGATAAAGGTTATTTTTTAATGGTAGAAGCAGG
TCGTATCGATCACGCTCATCATGCGGGTAATGCGGCGGGTGCACTGACTGATACTATTGAGTTAAGTGAAGCGGTACAGC
AGGCAATCAAGCAAACCAATCCGGCAGAGACCTTAATTATGGTCACCGCTGATCACAGTCATGTATTTACCATCGCAGGC
TACCCAAAACGTGGTAATCCTATCCTTGGTAAAGTAGTGAGTATCGGCAGTGATACCCCAACCCTTGCAAATGATGATTT
ACCCTATACCACTTTAGGTTACAGCAATGGTCTAGGCTTTCGCGATTTAGGGACAGAAACTAATGCTGACGTCAGCTACG
ACAGTCCTGCCATTGCCGGACGTGTAGATTTAAACGCGGTTAATACAGAAAGTAGCGGCTTTCATCAAGAAGCGACTGTG
CCGCTTGGTTCCGAAACCCATTCCGGTGAAGACGTCGCTTTATATGCTAGCGGTCCCGGTGCGCAGTTGGCCCAGGGAAA
TATTGAACAGAACGTGATTTATCACATTATCGAAAACGCATTAGATTTAGTTGGCAAGGAATAA

Upstream 100 bases:

>100_bases
TTATGTAACAATTCCACGGCACAATTGCATCAAATAATTATAAATTTCAAAGCAACTGCAACAAGGCAGTCGCTAAAAGA
CCTTCCAAGGGAGTGATGAT

Downstream 100 bases:

>100_bases
GGTAACGATATGAAAACTAACAATAACATAAGTCTATTTAAACCGAGTCTAATCGCTGTTGCCGTACTCGCGTTAAGCGC
GTGTAATAATTTGCAGGAAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDVRKSLVSTLVTLAISSSVSAGVVPENQKNTWFQSANSEITEKLQQTYQKRAKNVILFVGDGMGISTLTAARILEGQLS
GNPGEEHQLSFETFPYSALVKTYNVDAQTPDSAGTMTAMMSGVKSDAGVIGVGEKVQRGDCSTMDNNELVSIVDLAEIKG
LSTGVVTTARITHATPAATYAKSVERNWEDISDMPDDAIAGGCKDIADQLVNYQSNLETRFPGLNVDGIEVLFGGGRRHF
LPKDAAFNSLDASSEKEGDRTDGRDLTSEWQNIYKDGTYVYEQTGFDAIDTESSSHVLGLFNESHMQYAADRTNDIAGEP
SLSQMTGKAINILDNNDKGYFLMVEAGRIDHAHHAGNAAGALTDTIELSEAVQQAIKQTNPAETLIMVTADHSHVFTIAG
YPKRGNPILGKVVSIGSDTPTLANDDLPYTTLGYSNGLGFRDLGTETNADVSYDSPAIAGRVDLNAVNTESSGFHQEATV
PLGSETHSGEDVALYASGPGAQLAQGNIEQNVIYHIIENALDLVGKE

Sequences:

>Translated_527_residues
MDVRKSLVSTLVTLAISSSVSAGVVPENQKNTWFQSANSEITEKLQQTYQKRAKNVILFVGDGMGISTLTAARILEGQLS
GNPGEEHQLSFETFPYSALVKTYNVDAQTPDSAGTMTAMMSGVKSDAGVIGVGEKVQRGDCSTMDNNELVSIVDLAEIKG
LSTGVVTTARITHATPAATYAKSVERNWEDISDMPDDAIAGGCKDIADQLVNYQSNLETRFPGLNVDGIEVLFGGGRRHF
LPKDAAFNSLDASSEKEGDRTDGRDLTSEWQNIYKDGTYVYEQTGFDAIDTESSSHVLGLFNESHMQYAADRTNDIAGEP
SLSQMTGKAINILDNNDKGYFLMVEAGRIDHAHHAGNAAGALTDTIELSEAVQQAIKQTNPAETLIMVTADHSHVFTIAG
YPKRGNPILGKVVSIGSDTPTLANDDLPYTTLGYSNGLGFRDLGTETNADVSYDSPAIAGRVDLNAVNTESSGFHQEATV
PLGSETHSGEDVALYASGPGAQLAQGNIEQNVIYHIIENALDLVGKE
>Mature_527_residues
MDVRKSLVSTLVTLAISSSVSAGVVPENQKNTWFQSANSEITEKLQQTYQKRAKNVILFVGDGMGISTLTAARILEGQLS
GNPGEEHQLSFETFPYSALVKTYNVDAQTPDSAGTMTAMMSGVKSDAGVIGVGEKVQRGDCSTMDNNELVSIVDLAEIKG
LSTGVVTTARITHATPAATYAKSVERNWEDISDMPDDAIAGGCKDIADQLVNYQSNLETRFPGLNVDGIEVLFGGGRRHF
LPKDAAFNSLDASSEKEGDRTDGRDLTSEWQNIYKDGTYVYEQTGFDAIDTESSSHVLGLFNESHMQYAADRTNDIAGEP
SLSQMTGKAINILDNNDKGYFLMVEAGRIDHAHHAGNAAGALTDTIELSEAVQQAIKQTNPAETLIMVTADHSHVFTIAG
YPKRGNPILGKVVSIGSDTPTLANDDLPYTTLGYSNGLGFRDLGTETNADVSYDSPAIAGRVDLNAVNTESSGFHQEATV
PLGSETHSGEDVALYASGPGAQLAQGNIEQNVIYHIIENALDLVGKE

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=521, Percent_Identity=41.2667946257198, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI294660770, Length=466, Percent_Identity=42.4892703862661, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI157266292, Length=505, Percent_Identity=40.7920792079208, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI157266296, Length=505, Percent_Identity=39.8019801980198, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI94721246, Length=506, Percent_Identity=39.7233201581028, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI294660772, Length=422, Percent_Identity=40.2843601895735, Blast_Score=314, Evalue=1e-85,
Organism=Escherichia coli, GI48994877, Length=377, Percent_Identity=30.2387267904509, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320689, Length=353, Percent_Identity=32.0113314447592, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21355149, Length=499, Percent_Identity=44.0881763527054, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21355151, Length=503, Percent_Identity=40.5566600397614, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI21355981, Length=498, Percent_Identity=40.1606425702811, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21358067, Length=496, Percent_Identity=39.5161290322581, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI19921912, Length=511, Percent_Identity=39.5303326810176, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI85725200, Length=488, Percent_Identity=40.1639344262295, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI85815833, Length=488, Percent_Identity=40.1639344262295, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24657835, Length=516, Percent_Identity=39.7286821705426, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24657827, Length=514, Percent_Identity=37.3540856031128, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24651554, Length=486, Percent_Identity=39.0946502057613, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24657842, Length=495, Percent_Identity=38.989898989899, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24585213, Length=510, Percent_Identity=37.4509803921569, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI18859923, Length=522, Percent_Identity=34.6743295019157, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24651556, Length=468, Percent_Identity=38.4615384615385, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24645593, Length=327, Percent_Identity=29.0519877675841, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 56315; Mature: 56315

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRKSLVSTLVTLAISSSVSAGVVPENQKNTWFQSANSEITEKLQQTYQKRAKNVILFV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCEEEEEE
GDGMGISTLTAARILEGQLSGNPGEEHQLSFETFPYSALVKTYNVDAQTPDSAGTMTAMM
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHEEECCCCCCCCCCHHHHHHH
SGVKSDAGVIGVGEKVQRGDCSTMDNNELVSIVDLAEIKGLSTGVVTTARITHATPAATY
HCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEEEEECCCCHHHH
AKSVERNWEDISDMPDDAIAGGCKDIADQLVNYQSNLETRFPGLNVDGIEVLFGGGRRHF
HHHHHCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEECCCCCCC
LPKDAAFNSLDASSEKEGDRTDGRDLTSEWQNIYKDGTYVYEQTGFDAIDTESSSHVLGL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEE
FNESHMQYAADRTNDIAGEPSLSQMTGKAINILDNNDKGYFLMVEAGRIDHAHHAGNAAG
ECCHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEECCCCCEEEEEECCCCCCHHCCCCCCC
ALTDTIELSEAVQQAIKQTNPAETLIMVTADHSHVFTIAGYPKRGNPILGKVVSIGSDTP
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCEEHEEECCCCCC
TLANDDLPYTTLGYSNGLGFRDLGTETNADVSYDSPAIAGRVDLNAVNTESSGFHQEATV
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCC
PLGSETHSGEDVALYASGPGAQLAQGNIEQNVIYHIIENALDLVGKE
CCCCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVRKSLVSTLVTLAISSSVSAGVVPENQKNTWFQSANSEITEKLQQTYQKRAKNVILFV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCEEEEEE
GDGMGISTLTAARILEGQLSGNPGEEHQLSFETFPYSALVKTYNVDAQTPDSAGTMTAMM
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHEEECCCCCCCCCCHHHHHHH
SGVKSDAGVIGVGEKVQRGDCSTMDNNELVSIVDLAEIKGLSTGVVTTARITHATPAATY
HCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEEEEEECCCCHHHH
AKSVERNWEDISDMPDDAIAGGCKDIADQLVNYQSNLETRFPGLNVDGIEVLFGGGRRHF
HHHHHCCHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEECCCCCCC
LPKDAAFNSLDASSEKEGDRTDGRDLTSEWQNIYKDGTYVYEQTGFDAIDTESSSHVLGL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEE
FNESHMQYAADRTNDIAGEPSLSQMTGKAINILDNNDKGYFLMVEAGRIDHAHHAGNAAG
ECCHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEECCCCCEEEEEECCCCCCHHCCCCCCC
ALTDTIELSEAVQQAIKQTNPAETLIMVTADHSHVFTIAGYPKRGNPILGKVVSIGSDTP
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCEEHEEECCCCCC
TLANDDLPYTTLGYSNGLGFRDLGTETNADVSYDSPAIAGRVDLNAVNTESSGFHQEATV
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCC
PLGSETHSGEDVALYASGPGAQLAQGNIEQNVIYHIIENALDLVGKE
CCCCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]