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The map label for this gene is metE

Identifier: 119946991

GI number: 119946991

Start: 4183334

End: 4185637

Strand: Direct

Name: metE

Synonym: Ping_3386

Alternate gene names: 119946991

Gene position: 4183334-4185637 (Clockwise)

Preceding gene: 119946988

Following gene: 119946993

Centisome position: 91.75

GC content: 42.27

Gene sequence:

>2304_bases
ATGGCTAAACTACATCACTTAGGTTTCCCGCGTATTGGCAATAAACGTCAGCTTAAATTTGCTCTGGAAAAATTCTGGGG
TGGCGAATTAAGCGAACAGCAATTACAAACAAGCGCTGCCGATTTACGTCAACAAAACTGGCTGGATCAGCAAGACTTAG
CGCTGGATTTATATACCGTCGGGGACTTCTCTCTTTATGATCAGGTACTTGATATGAGTTTTTTAGTGGGTAATATTCCC
AGCCGTGCCAAACAAAGTGAGCAGGATAATAGTTTAGATAATTACTTTCGGGTGGCCCGTGGCCGATCTCAGCCACAGGA
ATGCTGCGCTGCCTCCCATGGACAATCGGGTGAGAGTCAGGCAGGAGAGATGACAAAATGGTTTAATACTAACTATCATT
ACATCGTTCCGGAGTTTGATGCGCAGACAACTTTCTTGTTAAATGCCAAGCAGCTGCTGCTACAAATTGAAGCGCTGCAG
AAGATTAATAAAAACGTTAAACCGGTGATCATCGGCCCAGTAACCTATCTGTGGTTAGGCAAAGAAAAAGATAACAGCCA
AAAACTCACGCTACTGCCAGAATTGCTGGAAGTTTACGCTGAATTATTGGCATTATTTGCAGAAAAAAATATTACCTGGG
TGCAAATCGACGAGCCTATTTTAGTTTTAGAAATCCCTAAGCAATGGCAGGAAGCACTGCAGAGTAGTTATCAGAAATTG
GGCCAATCCAAGGTTAAGCTTCTTTTAACCACTTATTTTGGCGAGCTTAAGAATAACTTAAAACTGGCCTGTGAACTGCC
CATTGCAGGTTTACACCTTGATGCGGTTAATGCGCCACATGAAATAGATTCTGCGATAGATTATTTAGGGACTGATAAAA
TATTATCTCTGGGTATTATCAATGCGTGTAATATTTGGAAAACAGATCTTAATCAAACGTTAAATCAATTGCTTCCGATA
CATCAGAAATTACAGGACAGGTTGTGGCTGGCACCCAGTTCATCATTATTACATGTCCCCATCGATCTAAATAATGAAGA
CAAACTTGATTACGAAGTTAAATCCTGGCTGGCTTTTGCGGTTCAAAAATTACAGGAATTAAGCTTAATTGGCCGGGCTT
TAAATGACGGTTTAAGCAGCGTTGAAGCGGCCTTTACTTATAATAAACTCTGTATTGAAAATCGTCGTCAAAGCCCTCAA
GTCCACAAACAAGATATTAAAAGCAAAGTGCTTAATATCACGCCTGAATTAGGGCAGCGTAACTTGGCTATAAAGCAACG
GGAAGCTTTACAGCAATCCTATTTAAATTTACCCAGCTATCCGACCACAACCATAGGATCTTTCCCGCAAACCAGTGATA
TTCGGCAATTACGTCAGGCCAAACGTTTAGGAGATATCAATGAAGAACAATATAAAAAAAGCATTAAAGAATCCATTGAA
TTTTGTATTAATGAGCAAGATCAGCTTGGCCTGGATGTCTTAGTGCACGGTGAAGCCGAACGGAATGATATGGTTGAATA
CTTCGGAGAGCTTCTTGACGGTTTTGTTTTCACCCAATTTGCCTGGGTGCAATCCTATGGTTCCCGCTGCGTTAAACCGC
CGATTTTATTTGGTGATGTTTCACGTCCTAAGGCAATGACCGTTGAATGGTCAAGCTACGCACAATCTCTGACTGAGAAA
AAGGTAAAAGGCATGCTTACGGGTCCGGTCACTATTTTAAACTGGTCTTTTGTGCGTGATGACCAGCCTCTGCAGACCAC
CTGTATGCAGATTGCGCTGGCCATTCGCGAGGAAGTGCTTGATTTAGAAAAGGCAGGGATTAATATTATCCAGATTGATG
AAGCGGCGCTGCGTGAAAAATTACCACTGCATCGCTCCCAATGGCAGCAATATTTAAACTGGGCAATCGCAGCCTTTCGA
CTCTGTGCTAACGGTGTTAATGATAAAACCCAGATTCACACCCACATGTGTTATTCCGAGTTTAACGATATTATTGCGGC
AATAGCACAAATGGATGCCGATGTCATTACCATTGAAACCTCTCGCTCGGATATGGTTTTATTAGATGTTTTTGAAGCGT
TTAGTTACCCTAATGGGATAGGACCCGGCGTTTATGATATCCATTCTCCCAACATACCCGATGTTGAGCAGATGGTAAGT
TTAATGAAAAAAGCAGCCCTGCGTATTCCGGCAGAGCAGCTTTGGGTTAATCCTGATTGCGGTTTAAAAACAAGGCGCTG
GGAAGAGGTTAAACCTGCATTACTAAACATGGTTGCAGCAGCCAAAAAACTGCGCGCCAGTTAA

Upstream 100 bases:

>100_bases
CATGGTTTTGTAATAAATCATCATTATTTTTCATAGTTAATTCTAAATACAATCATTCTCAACTTCAATGTCTGCTCTAT
TTAAAAGGATGATAAAAATA

Downstream 100 bases:

>100_bases
TAAAAATAGCCATAAGCTGTGAATAAAGAGGGTGAAAAGATTGCTGTTATCAACGCTAATCTCTTCACCCTTTTTGGGTA
TTATTTTAAACCAGCGGCGC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 767; Mature: 766

Protein sequence:

>767_residues
MAKLHHLGFPRIGNKRQLKFALEKFWGGELSEQQLQTSAADLRQQNWLDQQDLALDLYTVGDFSLYDQVLDMSFLVGNIP
SRAKQSEQDNSLDNYFRVARGRSQPQECCAASHGQSGESQAGEMTKWFNTNYHYIVPEFDAQTTFLLNAKQLLLQIEALQ
KINKNVKPVIIGPVTYLWLGKEKDNSQKLTLLPELLEVYAELLALFAEKNITWVQIDEPILVLEIPKQWQEALQSSYQKL
GQSKVKLLLTTYFGELKNNLKLACELPIAGLHLDAVNAPHEIDSAIDYLGTDKILSLGIINACNIWKTDLNQTLNQLLPI
HQKLQDRLWLAPSSSLLHVPIDLNNEDKLDYEVKSWLAFAVQKLQELSLIGRALNDGLSSVEAAFTYNKLCIENRRQSPQ
VHKQDIKSKVLNITPELGQRNLAIKQREALQQSYLNLPSYPTTTIGSFPQTSDIRQLRQAKRLGDINEEQYKKSIKESIE
FCINEQDQLGLDVLVHGEAERNDMVEYFGELLDGFVFTQFAWVQSYGSRCVKPPILFGDVSRPKAMTVEWSSYAQSLTEK
KVKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVLDLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFR
LCANGVNDKTQIHTHMCYSEFNDIIAAIAQMDADVITIETSRSDMVLLDVFEAFSYPNGIGPGVYDIHSPNIPDVEQMVS
LMKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAKKLRAS

Sequences:

>Translated_767_residues
MAKLHHLGFPRIGNKRQLKFALEKFWGGELSEQQLQTSAADLRQQNWLDQQDLALDLYTVGDFSLYDQVLDMSFLVGNIP
SRAKQSEQDNSLDNYFRVARGRSQPQECCAASHGQSGESQAGEMTKWFNTNYHYIVPEFDAQTTFLLNAKQLLLQIEALQ
KINKNVKPVIIGPVTYLWLGKEKDNSQKLTLLPELLEVYAELLALFAEKNITWVQIDEPILVLEIPKQWQEALQSSYQKL
GQSKVKLLLTTYFGELKNNLKLACELPIAGLHLDAVNAPHEIDSAIDYLGTDKILSLGIINACNIWKTDLNQTLNQLLPI
HQKLQDRLWLAPSSSLLHVPIDLNNEDKLDYEVKSWLAFAVQKLQELSLIGRALNDGLSSVEAAFTYNKLCIENRRQSPQ
VHKQDIKSKVLNITPELGQRNLAIKQREALQQSYLNLPSYPTTTIGSFPQTSDIRQLRQAKRLGDINEEQYKKSIKESIE
FCINEQDQLGLDVLVHGEAERNDMVEYFGELLDGFVFTQFAWVQSYGSRCVKPPILFGDVSRPKAMTVEWSSYAQSLTEK
KVKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVLDLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFR
LCANGVNDKTQIHTHMCYSEFNDIIAAIAQMDADVITIETSRSDMVLLDVFEAFSYPNGIGPGVYDIHSPNIPDVEQMVS
LMKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAKKLRAS
>Mature_766_residues
AKLHHLGFPRIGNKRQLKFALEKFWGGELSEQQLQTSAADLRQQNWLDQQDLALDLYTVGDFSLYDQVLDMSFLVGNIPS
RAKQSEQDNSLDNYFRVARGRSQPQECCAASHGQSGESQAGEMTKWFNTNYHYIVPEFDAQTTFLLNAKQLLLQIEALQK
INKNVKPVIIGPVTYLWLGKEKDNSQKLTLLPELLEVYAELLALFAEKNITWVQIDEPILVLEIPKQWQEALQSSYQKLG
QSKVKLLLTTYFGELKNNLKLACELPIAGLHLDAVNAPHEIDSAIDYLGTDKILSLGIINACNIWKTDLNQTLNQLLPIH
QKLQDRLWLAPSSSLLHVPIDLNNEDKLDYEVKSWLAFAVQKLQELSLIGRALNDGLSSVEAAFTYNKLCIENRRQSPQV
HKQDIKSKVLNITPELGQRNLAIKQREALQQSYLNLPSYPTTTIGSFPQTSDIRQLRQAKRLGDINEEQYKKSIKESIEF
CINEQDQLGLDVLVHGEAERNDMVEYFGELLDGFVFTQFAWVQSYGSRCVKPPILFGDVSRPKAMTVEWSSYAQSLTEKK
VKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVLDLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFRL
CANGVNDKTQIHTHMCYSEFNDIIAAIAQMDADVITIETSRSDMVLLDVFEAFSYPNGIGPGVYDIHSPNIPDVEQMVSL
MKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAKKLRAS

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=762, Percent_Identity=53.8057742782152, Blast_Score=809, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=766, Percent_Identity=46.0835509138381, Blast_Score=658, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_PSYIN (A1T007)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944671.1
- ProteinModelPortal:   A1T007
- SMR:   A1T007
- STRING:   A1T007
- GeneID:   4625674
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3386
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   A1T007
- ProtClustDB:   PRK05222
- BioCyc:   PING357804:PING_3386-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 87017; Mature: 86885

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLHHLGFPRIGNKRQLKFALEKFWGGELSEQQLQTSAADLRQQNWLDQQDLALDLYTV
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEEEC
GDFSLYDQVLDMSFLVGNIPSRAKQSEQDNSLDNYFRVARGRSQPQECCAASHGQSGESQ
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
AGEMTKWFNTNYHYIVPEFDAQTTFLLNAKQLLLQIEALQKINKNVKPVIIGPVTYLWLG
HCCHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEC
KEKDNSQKLTLLPELLEVYAELLALFAEKNITWVQIDEPILVLEIPKQWQEALQSSYQKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHH
GQSKVKLLLTTYFGELKNNLKLACELPIAGLHLDAVNAPHEIDSAIDYLGTDKILSLGII
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
NACNIWKTDLNQTLNQLLPIHQKLQDRLWLAPSSSLLHVPIDLNNEDKLDYEVKSWLAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
VQKLQELSLIGRALNDGLSSVEAAFTYNKLCIENRRQSPQVHKQDIKSKVLNITPELGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCC
NLAIKQREALQQSYLNLPSYPTTTIGSFPQTSDIRQLRQAKRLGDINEEQYKKSIKESIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FCINEQDQLGLDVLVHGEAERNDMVEYFGELLDGFVFTQFAWVQSYGSRCVKPPILFGDV
HHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
SRPKAMTVEWSSYAQSLTEKKVKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVL
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
DLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFRLCANGVNDKTQIHTHMCYSE
HHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FNDIIAAIAQMDADVITIETSRSDMVLLDVFEAFSYPNGIGPGVYDIHSPNIPDVEQMVS
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHH
LMKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAKKLRAS
HHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKLHHLGFPRIGNKRQLKFALEKFWGGELSEQQLQTSAADLRQQNWLDQQDLALDLYTV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEEEC
GDFSLYDQVLDMSFLVGNIPSRAKQSEQDNSLDNYFRVARGRSQPQECCAASHGQSGESQ
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
AGEMTKWFNTNYHYIVPEFDAQTTFLLNAKQLLLQIEALQKINKNVKPVIIGPVTYLWLG
HCCHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEC
KEKDNSQKLTLLPELLEVYAELLALFAEKNITWVQIDEPILVLEIPKQWQEALQSSYQKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHH
GQSKVKLLLTTYFGELKNNLKLACELPIAGLHLDAVNAPHEIDSAIDYLGTDKILSLGII
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
NACNIWKTDLNQTLNQLLPIHQKLQDRLWLAPSSSLLHVPIDLNNEDKLDYEVKSWLAFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
VQKLQELSLIGRALNDGLSSVEAAFTYNKLCIENRRQSPQVHKQDIKSKVLNITPELGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCC
NLAIKQREALQQSYLNLPSYPTTTIGSFPQTSDIRQLRQAKRLGDINEEQYKKSIKESIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FCINEQDQLGLDVLVHGEAERNDMVEYFGELLDGFVFTQFAWVQSYGSRCVKPPILFGDV
HHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
SRPKAMTVEWSSYAQSLTEKKVKGMLTGPVTILNWSFVRDDQPLQTTCMQIALAIREEVL
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
DLEKAGINIIQIDEAALREKLPLHRSQWQQYLNWAIAAFRLCANGVNDKTQIHTHMCYSE
HHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FNDIIAAIAQMDADVITIETSRSDMVLLDVFEAFSYPNGIGPGVYDIHSPNIPDVEQMVS
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHH
LMKKAALRIPAEQLWVNPDCGLKTRRWEEVKPALLNMVAAAKKLRAS
HHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA