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The map label for this gene is metK

Identifier: 119946833

GI number: 119946833

Start: 3982110

End: 3983258

Strand: Direct

Name: metK

Synonym: Ping_3227

Alternate gene names: 119946833

Gene position: 3982110-3983258 (Clockwise)

Preceding gene: 119946830

Following gene: 119946834

Centisome position: 87.33

GC content: 44.65

Gene sequence:

>1149_bases
ATGTCACGTGACTTATTTACTTCAGAATCCGTCTCTGAAGGTCATCCCGATAAAATCGCCGATCAAATTTCCGATGCGGT
ACTTGATGCAATTTTAAAACAAGATAAAAAAGCACGTGTTGCTTGTGAGACTTATGTAAAAACCGGCATGGTAATGGTTG
GTGGTGAAATCACCACTGATGCCTGGGTTGATATCGAAGAGATCACCCGTAATACCGTACGCAACATAGGTTACACCAGT
TCAGAAATGGGCTTTGACGCTGACTCATGCGCCATTTTAAATGTAATAGGCAAACAATCGCCAGACATTAATCAGGGTGT
TGACCGTAGTGATCCTCGCGAGCAGGGCGCTGGAGACCAAGGTTTAATGTTCGGTTATGCCACGAATGAAACAGACGTTT
TTATGCCGGCACCTATTACTTATGCGCACCACCTGGTTAAACGACAAGCAAAAGTGCGTAAAAATAAGACGCTTCCATGG
TTACGCCCCGACGCTAAAAGTCAGGTGACCTTTGCTTATGATCATGGCAAAATTGTTGGTATTGATGCGGTCGTCCTTTC
CACTCAACACTCAGAAGATATCAAACAAGAAGATTTAGTTGAAGCGGTAATGGAAGAGATAATCAAACCCGTATTACCCG
CCGAGTGGCTTTCTGAGCGCACCAAATACTTTATTAATCCAACGGGCCGCTTCGTCATTGGCGGACCTGTTGGTGATTGT
GGCCTTACCGGGCGTAAAATTATTGTTGATACTTATGGTGGTTCAGCACGCCATGGTGGTGGTGCATTCTCCGGTAAAGA
TCCCTCAAAAGTTGACCGCAGCGCGGCATATGCAGCCCGTTATGTGGCAAAAAACATCGTTGCAGCGGGTCTTGCTGATC
GTTGTGAAATTCAGGTTTCTTATGCCATAGGTGTTGCAGAGCCAACCTCGATCAGTGTCGAAACATTCGGCACAGAAAAA
GTAAAAAAAGATTTAATCATCCAATTGATTCGTGAGCATTTTGACTTACGTCCTTATGGACTGATCGAGATGTTGAATCT
TATTCAGCCCATTTACCAATCAACAGCCGCTTATGGGCACTTTGGTCGTAATGAATTCCCATGGGAAGCCTTAGATAAAG
TTGAAGCGTTAAAAGCGGGCGCATTTTAG

Upstream 100 bases:

>100_bases
TTAAATAATTAAGGTGTTTTCCATCACAAAAAAATGTAAGATAGACGTCTAGATGTAAAAACATCCAAAAAATTTACCTT
GTAACCAGTTAGGAGCTCAC

Downstream 100 bases:

>100_bases
CGCTTAGTTAAATTAAACATGGTAAATGACGCAGATAAAAAAGCACTCATTAGCCGAAGTGAAAAATTTTTCATTTTGGC
CGAGTGCTTTTTTGATCGTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSRDLFTSESVSEGHPDKIADQISDAVLDAILKQDKKARVACETYVKTGMVMVGGEITTDAWVDIEEITRNTVRNIGYTS
SEMGFDADSCAILNVIGKQSPDINQGVDRSDPREQGAGDQGLMFGYATNETDVFMPAPITYAHHLVKRQAKVRKNKTLPW
LRPDAKSQVTFAYDHGKIVGIDAVVLSTQHSEDIKQEDLVEAVMEEIIKPVLPAEWLSERTKYFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTEK
VKKDLIIQLIREHFDLRPYGLIEMLNLIQPIYQSTAAYGHFGRNEFPWEALDKVEALKAGAF

Sequences:

>Translated_382_residues
MSRDLFTSESVSEGHPDKIADQISDAVLDAILKQDKKARVACETYVKTGMVMVGGEITTDAWVDIEEITRNTVRNIGYTS
SEMGFDADSCAILNVIGKQSPDINQGVDRSDPREQGAGDQGLMFGYATNETDVFMPAPITYAHHLVKRQAKVRKNKTLPW
LRPDAKSQVTFAYDHGKIVGIDAVVLSTQHSEDIKQEDLVEAVMEEIIKPVLPAEWLSERTKYFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTEK
VKKDLIIQLIREHFDLRPYGLIEMLNLIQPIYQSTAAYGHFGRNEFPWEALDKVEALKAGAF
>Mature_381_residues
SRDLFTSESVSEGHPDKIADQISDAVLDAILKQDKKARVACETYVKTGMVMVGGEITTDAWVDIEEITRNTVRNIGYTSS
EMGFDADSCAILNVIGKQSPDINQGVDRSDPREQGAGDQGLMFGYATNETDVFMPAPITYAHHLVKRQAKVRKNKTLPWL
RPDAKSQVTFAYDHGKIVGIDAVVLSTQHSEDIKQEDLVEAVMEEIIKPVLPAEWLSERTKYFINPTGRFVIGGPVGDCG
LTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTEKV
KKDLIIQLIREHFDLRPYGLIEMLNLIQPIYQSTAAYGHFGRNEFPWEALDKVEALKAGAF

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=57.9787234042553, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=56.4986737400531, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=83.7270341207349, Blast_Score=669, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=55.4959785522788, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=370, Percent_Identity=55.4054054054054, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538498, Length=370, Percent_Identity=55.4054054054054, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=382, Percent_Identity=54.1884816753927, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565905, Length=370, Percent_Identity=49.4594594594595, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=52, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=331, Percent_Identity=51.0574018126888, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=51.6778523489933, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=64.1975308641975, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=72.7272727272727, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=58.1081081081081, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=56.9148936170213, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.0531914893617, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=55.0531914893617, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=55.0531914893617, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=52.6455026455027, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=57.2649572649573, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PSYIN (A1SZJ9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944513.1
- ProteinModelPortal:   A1SZJ9
- SMR:   A1SZJ9
- STRING:   A1SZJ9
- GeneID:   4624660
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3227
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A1SZJ9
- ProtClustDB:   PRK05250
- BioCyc:   PING357804:PING_3227-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41879; Mature: 41748

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDLFTSESVSEGHPDKIADQISDAVLDAILKQDKKARVACETYVKTGMVMVGGEITTD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCEECCC
AWVDIEEITRNTVRNIGYTSSEMGFDADSCAILNVIGKQSPDINQGVDRSDPREQGAGDQ
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GLMFGYATNETDVFMPAPITYAHHLVKRQAKVRKNKTLPWLRPDAKSQVTFAYDHGKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHSEDIKQEDLVEAVMEEIIKPVLPAEWLSERTKYFINPTGRFVIGGPVGDC
EEEEEEECCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISVETFGTEKVKKDLIIQLIREHFDLRPYGLIEMLNLIQPIYQSTAAYGH
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGRNEFPWEALDKVEALKAGAF
CCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRDLFTSESVSEGHPDKIADQISDAVLDAILKQDKKARVACETYVKTGMVMVGGEITTD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCEECCC
AWVDIEEITRNTVRNIGYTSSEMGFDADSCAILNVIGKQSPDINQGVDRSDPREQGAGDQ
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
GLMFGYATNETDVFMPAPITYAHHLVKRQAKVRKNKTLPWLRPDAKSQVTFAYDHGKIVG
CEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHSEDIKQEDLVEAVMEEIIKPVLPAEWLSERTKYFINPTGRFVIGGPVGDC
EEEEEEECCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISVETFGTEKVKKDLIIQLIREHFDLRPYGLIEMLNLIQPIYQSTAAYGH
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGRNEFPWEALDKVEALKAGAF
CCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA