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The map label for this gene is glpK

Identifier: 119946775

GI number: 119946775

Start: 3898618

End: 3900141

Strand: Direct

Name: glpK

Synonym: Ping_3168

Alternate gene names: 119946775

Gene position: 3898618-3900141 (Clockwise)

Preceding gene: 119946774

Following gene: 119946782

Centisome position: 85.5

GC content: 46.39

Gene sequence:

>1524_bases
ATGACAGATAAAAAATACATTGTTGCATTGGACCAAGGTACCACCAGTTCTCGCGCTGTTATTCTTGACCACGATGCAAA
TATCGTCAGTGTTTCTCAACGTGAATTCAAACAAATCTATCCACAGGCCGGTTGGGTTGAGCATGATCCGATGGAACTTT
ATGCAACACAAAGTGCGGTATTAACTGAAATATTAGCGAAAGAAAGCATTCGCAGTGATCAAATAGCTGCCATCGGTATT
ACTAATCAGCGTGAAACAACCATTGTCTGGAATAGAGAGACAGGCATACCTGTCTATAATGCGATTGTGTGGCAGTGCCG
TCGTACGGCTTCTATCTGTGATGCACTAAAAGAACAGCCTGGTTTAGAAGACTATATCCGTGAAAACACCGGTCTGGTGC
TTGACCCCTACTTCTCCGGTACCAAAATTAAATGGATATTAGATAATGTTGAAGGCGCCCGCGAAGATGCTGAAGCAGGT
AAGTTAATGTTTGGTACCGTTGATACCTGGTTAGTTTGGAAAATGACCCAGAGGCACGTACACGTCACTGACTATACCAA
TGCTTCGCGCACTATGCTGTTTAATATTAATACCTTAAAATGGGATGAAAAACTCCTGAAAATATTAGGTATTCCGCTCT
CTATGATGGCTGAGGTAAAGAGCTCATCGGAAGTTTACGGTCAGACCAACATTGGTGGCGTTGGCGGTACACGTATTCCT
ATTGCGGGTATTGCCGGTGATCAACAGGCGGCGTTATATGGTCATATGTGTGTCGAGAAAGGTCAGGCTAAAAATACCTA
TGGCACGGGCTGCTTCTTATTAATGAATACCGGTAAAGAGAAAGTAACCTCGAGCAACGGTCTGTTAACAACCTTGGCCT
GCGGCCCGAAAGGCGAAGCCTCATATGCATTGGAAGGTGCGGTGTTTATGGGTGGCGCTTCTATTCAGTGGCTGCGGGAC
GAAATGAAATTACTCGCCGATGCCAAAGATTCTGAATACTTTGCAACTAAAGTTGATTCTTCAAACGGTGTTTACGTGGT
GCCGGCATTTACAGGCTTAGGCGCGCCTTATTGGGACCCCTATGCACGAGGCACTATCGTCGGTTTAACACGCGGCGTAA
ATTCAAACCATATTATTCGCGCAACATTAGAAAGTATTGCCTACCAAACGCGTGATGTGCTCGATGCAATGCAGGCCGAT
TCTGGTATTAAGCTGTCTGCGTTGCGTGTTGATGGTGGCGCCGTAGCGAATAACTTTTTAATGCAGTTCCAGTCCGATGT
ATTAGATACTACGGTTCAGCGCCCCGCTGTCTCCGAAGTAACAGCACTGGGAGCCGCTTATCTCGCCGGTCTTGCTGTCG
GTTACTGGGAGAGCTTGGAAGAGCTTGCAGGTAAAGCCGTCATTGAACAGACATTCGAACCTCATGCGGATCCGGTTAAA
CGCAAGCAACGTTACCGTGGTTGGAAACGCGCAGTAAAATGCACGCAGGCATGGGCCGAAATGCATGACGAAGATTTTGA
ATAA

Upstream 100 bases:

>100_bases
AGGCCTGATAAAGATAAAAGCATAGTCAGTAGAGGTTTAATTAACGAATCAGTAGTCAGACAAATTACAATTAACCAATT
TATAGACAAAGGACAATATT

Downstream 100 bases:

>100_bases
CTGCATTAGACTTTATATCAGTAGAAAATATCACTAACTAATATAGCCAGTATTAACCTCAAAAAGAGTGTCTTAGGACA
CTCTTTTTTGTATCTTCAAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTDKKYIVALDQGTTSSRAVILDHDANIVSVSQREFKQIYPQAGWVEHDPMELYATQSAVLTEILAKESIRSDQIAAIGI
TNQRETTIVWNRETGIPVYNAIVWQCRRTASICDALKEQPGLEDYIRENTGLVLDPYFSGTKIKWILDNVEGAREDAEAG
KLMFGTVDTWLVWKMTQRHVHVTDYTNASRTMLFNINTLKWDEKLLKILGIPLSMMAEVKSSSEVYGQTNIGGVGGTRIP
IAGIAGDQQAALYGHMCVEKGQAKNTYGTGCFLLMNTGKEKVTSSNGLLTTLACGPKGEASYALEGAVFMGGASIQWLRD
EMKLLADAKDSEYFATKVDSSNGVYVVPAFTGLGAPYWDPYARGTIVGLTRGVNSNHIIRATLESIAYQTRDVLDAMQAD
SGIKLSALRVDGGAVANNFLMQFQSDVLDTTVQRPAVSEVTALGAAYLAGLAVGYWESLEELAGKAVIEQTFEPHADPVK
RKQRYRGWKRAVKCTQAWAEMHDEDFE

Sequences:

>Translated_507_residues
MTDKKYIVALDQGTTSSRAVILDHDANIVSVSQREFKQIYPQAGWVEHDPMELYATQSAVLTEILAKESIRSDQIAAIGI
TNQRETTIVWNRETGIPVYNAIVWQCRRTASICDALKEQPGLEDYIRENTGLVLDPYFSGTKIKWILDNVEGAREDAEAG
KLMFGTVDTWLVWKMTQRHVHVTDYTNASRTMLFNINTLKWDEKLLKILGIPLSMMAEVKSSSEVYGQTNIGGVGGTRIP
IAGIAGDQQAALYGHMCVEKGQAKNTYGTGCFLLMNTGKEKVTSSNGLLTTLACGPKGEASYALEGAVFMGGASIQWLRD
EMKLLADAKDSEYFATKVDSSNGVYVVPAFTGLGAPYWDPYARGTIVGLTRGVNSNHIIRATLESIAYQTRDVLDAMQAD
SGIKLSALRVDGGAVANNFLMQFQSDVLDTTVQRPAVSEVTALGAAYLAGLAVGYWESLEELAGKAVIEQTFEPHADPVK
RKQRYRGWKRAVKCTQAWAEMHDEDFE
>Mature_506_residues
TDKKYIVALDQGTTSSRAVILDHDANIVSVSQREFKQIYPQAGWVEHDPMELYATQSAVLTEILAKESIRSDQIAAIGIT
NQRETTIVWNRETGIPVYNAIVWQCRRTASICDALKEQPGLEDYIRENTGLVLDPYFSGTKIKWILDNVEGAREDAEAGK
LMFGTVDTWLVWKMTQRHVHVTDYTNASRTMLFNINTLKWDEKLLKILGIPLSMMAEVKSSSEVYGQTNIGGVGGTRIPI
AGIAGDQQAALYGHMCVEKGQAKNTYGTGCFLLMNTGKEKVTSSNGLLTTLACGPKGEASYALEGAVFMGGASIQWLRDE
MKLLADAKDSEYFATKVDSSNGVYVVPAFTGLGAPYWDPYARGTIVGLTRGVNSNHIIRATLESIAYQTRDVLDAMQADS
GIKLSALRVDGGAVANNFLMQFQSDVLDTTVQRPAVSEVTALGAAYLAGLAVGYWESLEELAGKAVIEQTFEPHADPVKR
KQRYRGWKRAVKCTQAWAEMHDEDFE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=46.4143426294821, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=46.4143426294821, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=508, Percent_Identity=46.0629921259843, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.6215139442231, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=32.4803149606299, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1790361, Length=504, Percent_Identity=77.5793650793651, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=499, Percent_Identity=22.4448897795591, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787789, Length=507, Percent_Identity=25.2465483234714, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789138, Length=489, Percent_Identity=21.881390593047, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789168, Length=496, Percent_Identity=21.9758064516129, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=41.2698412698413, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=26.7307692307692, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=302, Percent_Identity=31.4569536423841, Blast_Score=131, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=547, Percent_Identity=37.1115173674589, Blast_Score=315, Evalue=8e-87,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=45.2569169960474, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655555, Length=499, Percent_Identity=41.6833667334669, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655560, Length=499, Percent_Identity=41.6833667334669, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655570, Length=499, Percent_Identity=41.6833667334669, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655574, Length=499, Percent_Identity=41.6833667334669, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655565, Length=499, Percent_Identity=41.6833667334669, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=47.3684210526316, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922058, Length=539, Percent_Identity=34.3228200371057, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24652871, Length=506, Percent_Identity=34.5849802371541, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=26.8482490272374, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=26.0952380952381, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=29.2263610315186, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45552909, Length=289, Percent_Identity=28.3737024221453, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_PSYIN (A1SZE1)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944455.1
- ProteinModelPortal:   A1SZE1
- SMR:   A1SZE1
- STRING:   A1SZE1
- GeneID:   4624714
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3168
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- PhylomeDB:   A1SZE1
- ProtClustDB:   PRK00047
- BioCyc:   PING357804:PING_3168-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55786; Mature: 55655

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 137-137 BINDING 247-247 BINDING 269-269 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKYIVALDQGTTSSRAVILDHDANIVSVSQREFKQIYPQAGWVEHDPMELYATQSAV
CCCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LTEILAKESIRSDQIAAIGITNQRETTIVWNRETGIPVYNAIVWQCRRTASICDALKEQP
HHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GLEDYIRENTGLVLDPYFSGTKIKWILDNVEGAREDAEAGKLMFGTVDTWLVWKMTQRHV
CHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHCCCEEEEEHHHHEEEEECCCEE
HVTDYTNASRTMLFNINTLKWDEKLLKILGIPLSMMAEVKSSSEVYGQTNIGGVGGTRIP
EEEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCCCCCCCCCCEE
IAGIAGDQQAALYGHMCVEKGQAKNTYGTGCFLLMNTGKEKVTSSNGLLTTLACGPKGEA
EEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEECCCCCCC
SYALEGAVFMGGASIQWLRDEMKLLADAKDSEYFATKVDSSNGVYVVPAFTGLGAPYWDP
CEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCC
YARGTIVGLTRGVNSNHIIRATLESIAYQTRDVLDAMQADSGIKLSALRVDGGAVANNFL
CCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
MQFQSDVLDTTVQRPAVSEVTALGAAYLAGLAVGYWESLEELAGKAVIEQTFEPHADPVK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RKQRYRGWKRAVKCTQAWAEMHDEDFE
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDKKYIVALDQGTTSSRAVILDHDANIVSVSQREFKQIYPQAGWVEHDPMELYATQSAV
CCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LTEILAKESIRSDQIAAIGITNQRETTIVWNRETGIPVYNAIVWQCRRTASICDALKEQP
HHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GLEDYIRENTGLVLDPYFSGTKIKWILDNVEGAREDAEAGKLMFGTVDTWLVWKMTQRHV
CHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHCCCEEEEEHHHHEEEEECCCEE
HVTDYTNASRTMLFNINTLKWDEKLLKILGIPLSMMAEVKSSSEVYGQTNIGGVGGTRIP
EEEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCCCCCCCCCCEE
IAGIAGDQQAALYGHMCVEKGQAKNTYGTGCFLLMNTGKEKVTSSNGLLTTLACGPKGEA
EEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEECCCCCCC
SYALEGAVFMGGASIQWLRDEMKLLADAKDSEYFATKVDSSNGVYVVPAFTGLGAPYWDP
CEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCC
YARGTIVGLTRGVNSNHIIRATLESIAYQTRDVLDAMQADSGIKLSALRVDGGAVANNFL
CCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
MQFQSDVLDTTVQRPAVSEVTALGAAYLAGLAVGYWESLEELAGKAVIEQTFEPHADPVK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RKQRYRGWKRAVKCTQAWAEMHDEDFE
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA