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The map label for this gene is lipB

Identifier: 119946650

GI number: 119946650

Start: 3726839

End: 3727489

Strand: Direct

Name: lipB

Synonym: Ping_3029

Alternate gene names: 119946650

Gene position: 3726839-3727489 (Clockwise)

Preceding gene: 119946649

Following gene: 119946651

Centisome position: 81.74

GC content: 42.24

Gene sequence:

>651_bases
ATGAAAACATTGGATACACAGCTTAAATTACGTTACTTAGGTAGACAAGATTACCTAACCTCGTGGCAGGCAATGAGCGA
CTTCACTAATCAACGTGATGAAAATACCATCGATGAAATATGGTTAGTCGAGCACCCTGCGGTGTTTACCCAAGGGCTTG
CGGGCAAAGCTGAACACCTGTTAAAGCACAGTGAAATCCCGATCGTGCAAAGCGATCGTGGTGGACAAATCACTTTTCAT
GCACCCGGACAGTTGGTGGTATACTTACTGATTAACCTTCGCCGCAAAGCGCTTAATGTACGCGCATTAGTCACGGTTAT
GGAAGACAGCATTATTAATTTATTAGCAGATTATAATGTTATCGCAGTGGCTAAACCCGATGCGCCAGGTGTCTATGTTA
ATGGTAAAAAAATAGCATCACTGGGACTGAAGATTCGCAAAGGCTGTTCTTTCCACGGATTGGCATTAAATGTTGATATG
GATTTATCCCCTTTTTTGCAAATTAATCCCTGTGGTTACGCGGGACTGGAGATGACCCAATGTAAGGCTGAGGGGTTAAC
GCTTTCAGTTGACGAACTCGCACCGTTATTGATTGAAAAAATGAATCAACAATTAGACTATTCACATATTGAGAGTTTTC
ACCATGAGTAA

Upstream 100 bases:

>100_bases
CCTGTGACTGTCACCAGCAAAGACCACATTGAAACGATTTACACTAAGGTGAGCGAACTTGAACTTGTTCGCCATATTCT
TTAACACTAATGGTTACTGA

Downstream 100 bases:

>100_bases
TAAACCAAGTCAATTAACTGCAGGCAAAAAACTACGCGACGCTGATAAAATGTCTCATATTCCGATTAAAGTTGTCCCTT
CGAACAAATCGACGCTATTA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKTLDTQLKLRYLGRQDYLTSWQAMSDFTNQRDENTIDEIWLVEHPAVFTQGLAGKAEHLLKHSEIPIVQSDRGGQITFH
APGQLVVYLLINLRRKALNVRALVTVMEDSIINLLADYNVIAVAKPDAPGVYVNGKKIASLGLKIRKGCSFHGLALNVDM
DLSPFLQINPCGYAGLEMTQCKAEGLTLSVDELAPLLIEKMNQQLDYSHIESFHHE

Sequences:

>Translated_216_residues
MKTLDTQLKLRYLGRQDYLTSWQAMSDFTNQRDENTIDEIWLVEHPAVFTQGLAGKAEHLLKHSEIPIVQSDRGGQITFH
APGQLVVYLLINLRRKALNVRALVTVMEDSIINLLADYNVIAVAKPDAPGVYVNGKKIASLGLKIRKGCSFHGLALNVDM
DLSPFLQINPCGYAGLEMTQCKAEGLTLSVDELAPLLIEKMNQQLDYSHIESFHHE
>Mature_216_residues
MKTLDTQLKLRYLGRQDYLTSWQAMSDFTNQRDENTIDEIWLVEHPAVFTQGLAGKAEHLLKHSEIPIVQSDRGGQITFH
APGQLVVYLLINLRRKALNVRALVTVMEDSIINLLADYNVIAVAKPDAPGVYVNGKKIASLGLKIRKGCSFHGLALNVDM
DLSPFLQINPCGYAGLEMTQCKAEGLTLSVDELAPLLIEKMNQQLDYSHIESFHHE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=192, Percent_Identity=58.3333333333333, Blast_Score=243, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6323268, Length=117, Percent_Identity=37.6068376068376, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355483, Length=148, Percent_Identity=31.7567567567568, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PSYIN (A1SZ16)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944330.1
- ProteinModelPortal:   A1SZ16
- SMR:   A1SZ16
- STRING:   A1SZ16
- GeneID:   4625115
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3029
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- PhylomeDB:   A1SZ16
- BioCyc:   PING357804:PING_3029-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24170; Mature: 24170

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLDTQLKLRYLGRQDYLTSWQAMSDFTNQRDENTIDEIWLVEHPAVFTQGLAGKAEHL
CCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCHHHHHCCCHHHHHH
LKHSEIPIVQSDRGGQITFHAPGQLVVYLLINLRRKALNVRALVTVMEDSIINLLADYNV
HHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IAVAKPDAPGVYVNGKKIASLGLKIRKGCSFHGLALNVDMDLSPFLQINPCGYAGLEMTQ
EEEECCCCCCEEECCHHHHHHCHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEE
CKAEGLTLSVDELAPLLIEKMNQQLDYSHIESFHHE
HHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MKTLDTQLKLRYLGRQDYLTSWQAMSDFTNQRDENTIDEIWLVEHPAVFTQGLAGKAEHL
CCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCHHHHHCCCHHHHHH
LKHSEIPIVQSDRGGQITFHAPGQLVVYLLINLRRKALNVRALVTVMEDSIINLLADYNV
HHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IAVAKPDAPGVYVNGKKIASLGLKIRKGCSFHGLALNVDMDLSPFLQINPCGYAGLEMTQ
EEEECCCCCCEEECCHHHHHHCHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEE
CKAEGLTLSVDELAPLLIEKMNQQLDYSHIESFHHE
HHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA