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The map label for this gene is lipA

Identifier: 119946651

GI number: 119946651

Start: 3727482

End: 3728450

Strand: Direct

Name: lipA

Synonym: Ping_3030

Alternate gene names: 119946651

Gene position: 3727482-3728450 (Clockwise)

Preceding gene: 119946650

Following gene: 119946652

Centisome position: 81.75

GC content: 41.28

Gene sequence:

>969_bases
ATGAGTAATAAACCAAGTCAATTAACTGCAGGCAAAAAACTACGCGACGCTGATAAAATGTCTCATATTCCGATTAAAGT
TGTCCCTTCGAACAAATCGACGCTATTAAAAAAACCATCCTGGATGAAAATAAAACTTTCTTCTGACAATACACGCGTCA
ATGAAATAAAAGCAGCATTACGTAAAAACAACCTTCATTCAGTTTGTGAAGAGGCCTCATGCCCAAACCTCAATGAGTGT
TTCAATCACGGCACCGCAACTTTTATGATTTTGGGTGATATTTGTACGCGCCGTTGCCCATTTTGTGATGTCGGTCATGG
TAAACCGCTGGCTGTCGATGCCAACGAACCTAAAAAACTGGCTGAAACCATTAAGGATATGAAACTAAAATATGTTGTTA
TCACCTCGGTGGATCGCGATGATCTTCGTGATGGAGGAGCGCAACATTTTGCCGATTGTATCCGTGAAATTCGCTTATTA
AACCCAGAGATTAAAATCGAAATTTTAGTGCCTGATTTTAAAGGCCGTATGGATAAAGCCTTGGCCTGTTTTGAGCAGGA
TCTGCCTGATGTATTTAATCATAACCTTGAAACAGCACCGCATTTATATAAGCAGGTTCGACCTGGCGCTGATTATAAAT
GGTCTTTAAAACTGCTTCAAAAATTCAAAGAGAAACATCCGCATATTCCAACAAAATCAGGTTTAATGGTGGGACTCGGT
GAAACAAATGAAGATATAGAGCAAGTACTTACTGACCTTAGAGCGCATGATGTTAATATGTTAACCCTAGGGCAATACTT
ACAACCTAGCCTGGACCATTTAGCGGTACAGCGTTTTGTTCCGCCCTCAGAGTTTGATGAATTAGGCGTAAAAGCCAAAG
CCCTCGGATTTGATCAAGCGGCTTGCGGCCCCTTAGTTCGCTCCTCATACCATGCGGATTTACAAGCATCTGGCCAGGAA
GTGAAATAA

Upstream 100 bases:

>100_bases
AAGGCTGAGGGGTTAACGCTTTCAGTTGACGAACTCGCACCGTTATTGATTGAAAAAATGAATCAACAATTAGACTATTC
ACATATTGAGAGTTTTCACC

Downstream 100 bases:

>100_bases
AATTACCCACGCCACTAGGAAAAGCGCATTGAATGCGCTTTTCCTGTTATATACGGCGCCTTTTTATCACCTTCCGCGTA
AGACAACAATATACCGCCAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSNKPSQLTAGKKLRDADKMSHIPIKVVPSNKSTLLKKPSWMKIKLSSDNTRVNEIKAALRKNNLHSVCEEASCPNLNEC
FNHGTATFMILGDICTRRCPFCDVGHGKPLAVDANEPKKLAETIKDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRLL
NPEIKIEILVPDFKGRMDKALACFEQDLPDVFNHNLETAPHLYKQVRPGADYKWSLKLLQKFKEKHPHIPTKSGLMVGLG
ETNEDIEQVLTDLRAHDVNMLTLGQYLQPSLDHLAVQRFVPPSEFDELGVKAKALGFDQAACGPLVRSSYHADLQASGQE
VK

Sequences:

>Translated_322_residues
MSNKPSQLTAGKKLRDADKMSHIPIKVVPSNKSTLLKKPSWMKIKLSSDNTRVNEIKAALRKNNLHSVCEEASCPNLNEC
FNHGTATFMILGDICTRRCPFCDVGHGKPLAVDANEPKKLAETIKDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRLL
NPEIKIEILVPDFKGRMDKALACFEQDLPDVFNHNLETAPHLYKQVRPGADYKWSLKLLQKFKEKHPHIPTKSGLMVGLG
ETNEDIEQVLTDLRAHDVNMLTLGQYLQPSLDHLAVQRFVPPSEFDELGVKAKALGFDQAACGPLVRSSYHADLQASGQE
VK
>Mature_321_residues
SNKPSQLTAGKKLRDADKMSHIPIKVVPSNKSTLLKKPSWMKIKLSSDNTRVNEIKAALRKNNLHSVCEEASCPNLNECF
NHGTATFMILGDICTRRCPFCDVGHGKPLAVDANEPKKLAETIKDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRLLN
PEIKIEILVPDFKGRMDKALACFEQDLPDVFNHNLETAPHLYKQVRPGADYKWSLKLLQKFKEKHPHIPTKSGLMVGLGE
TNEDIEQVLTDLRAHDVNMLTLGQYLQPSLDHLAVQRFVPPSEFDELGVKAKALGFDQAACGPLVRSSYHADLQASGQEV
K

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=288, Percent_Identity=43.4027777777778, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=43.6507936507937, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786846, Length=320, Percent_Identity=71.875, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=43.3566433566434, Blast_Score=224, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=41.9141914191419, Blast_Score=226, Evalue=3e-60,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=45.0704225352113, Blast_Score=237, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PSYIN (A1SZ17)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944331.1
- ProteinModelPortal:   A1SZ17
- STRING:   A1SZ17
- GeneID:   4625116
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3030
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   A1SZ17
- ProtClustDB:   PRK05481
- BioCyc:   PING357804:PING_3030-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36030; Mature: 35899

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPSQLTAGKKLRDADKMSHIPIKVVPSNKSTLLKKPSWMKIKLSSDNTRVNEIKAAL
CCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCEEEEEECCCCCHHHHHHHHH
RKNNLHSVCEEASCPNLNECFNHGTATFMILGDICTRRCPFCDVGHGKPLAVDANEPKKL
HHCCHHHHHHHCCCCCHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCEEEECCCCHHHH
AETIKDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRLLNPEIKIEILVPDFKGRMDKA
HHHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LACFEQDLPDVFNHNLETAPHLYKQVRPGADYKWSLKLLQKFKEKHPHIPTKSGLMVGLG
HHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECC
ETNEDIEQVLTDLRAHDVNMLTLGQYLQPSLDHLAVQRFVPPSEFDELGVKAKALGFDQA
CCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCHH
ACGPLVRSSYHADLQASGQEVK
HHCHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SNKPSQLTAGKKLRDADKMSHIPIKVVPSNKSTLLKKPSWMKIKLSSDNTRVNEIKAAL
CCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCEEEEEECCCCCHHHHHHHHH
RKNNLHSVCEEASCPNLNECFNHGTATFMILGDICTRRCPFCDVGHGKPLAVDANEPKKL
HHCCHHHHHHHCCCCCHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCEEEECCCCHHHH
AETIKDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRLLNPEIKIEILVPDFKGRMDKA
HHHHHHCEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
LACFEQDLPDVFNHNLETAPHLYKQVRPGADYKWSLKLLQKFKEKHPHIPTKSGLMVGLG
HHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECC
ETNEDIEQVLTDLRAHDVNMLTLGQYLQPSLDHLAVQRFVPPSEFDELGVKAKALGFDQA
CCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCHH
ACGPLVRSSYHADLQASGQEVK
HHCHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA