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The map label for this gene is amtB [C]

Identifier: 119946646

GI number: 119946646

Start: 3723298

End: 3724518

Strand: Reverse

Name: amtB [C]

Synonym: Ping_3025

Alternate gene names: 119946646

Gene position: 3724518-3723298 (Counterclockwise)

Preceding gene: 119946647

Following gene: 119946645

Centisome position: 81.69

GC content: 43.08

Gene sequence:

>1221_bases
ATGGAAAACACAGTAACAGAACTCAGGTTTGCGCTCGATACCTTCTATTTTTTAATATCAGGTGTCTTAGTCATGTGGAT
GGCGGCAGGTTTTGCAATGTTAGAGGCCGGGCTTGTTCGATCTAAAAACACCACTGAAATTTTAACTAAAAACATCGTGT
TATATGCCATCGCTTGTACCATGTTCCTGCTGGTTGGTTATAACATTATGTATGTTGATAATAGCGCAGGGGGCTGGTTA
CCTTCATTTGGCAGTTTGATAGGTATCCAAGCGGAAGGTGCTGACCATTCACTTGAATCAGATTTCTTCTTCCAAGTTGT
TTTTGTGGCAACAGCAATGTCAATCGTATCTGGCGCAGTGGCTGAACGTATGAAATTGTGGGCATTCTTAATATTCTCGG
TCTTTTTAACAGGTTTCATTTACCCAATGGAAGGATACTGGACTTGGGGCGGTGGTTTCCTTGCTGAAGCCGGTTTTAGT
GATTTTGCAGGTTCTGGTATTGTTCATATGGCAGGTGCTGCTGCGGCATTAGCAGCTGTTATATTACTGGGTGCACGTAA
AGGTAAATTTGGTAAAAATGGTTCAGTTCACCCTATCCCGGGTTCAAACATGCCACTTGCAACATTAGGTACACTTATTT
TATGGATGGGCTGGTTTGGTTTTAACGGTGGCTCACAATTAGCACTGTCGAATGCAGAAAATGCCAGTGCGGTTGGTTTG
ATTTTTGTTAATACCAATGCGGCGGCAGCAACAGGTGCCATTGCTGCTCTAATAGTCAGTAAACTTAAATGGGGTAAAGC
TGATTTAACCATGATTTTAAATGGTGCATTAGCAGGCTTAGTTGCCATTACTGCGGATCCTTTATCGCCTACTCCACTAT
TCTCAGCAGGTATCGGTGCGGTTGCAGGTATCATCGTTGTTTACAGTATCCTGACTTTTGATAAATTAAAGTTAGATGAT
CCGGTAGGTGCCTTATCTGTGCATGGTGTCTGTGGTCTGTTTGGTCTATTAGTGGTGCCACTAAATAATGAGGGTGCTTC
TTTTGCGACCCAATTATACGGTGCTGCGGTTATCTTCGGTTTTGTCTTTGTTGCGTCATGGATTGTCTGGAGCGTTATTA
AAGTCACTATGGGCGTTCGTGTTACCGCTGAAGAAGAAGAAACAGGTATGGATTTACACGATTGTGGTGTAGAGGCTTAT
CCGGAGTTCACTGCTCGATAA

Upstream 100 bases:

>100_bases
ATTTATTCATCCCCTTGAGCAAGTTGTGCGTATTCGTACCGGTGAGCTTGATGAAGAAGCCCTTTAATCTATTAAGTAAT
TTTTAAAAAGAGGAATTATT

Downstream 100 bases:

>100_bases
CAGCTAAAAAAGTCATTTAATTAGCCCTGCCTTATTACTAAGTCAGGGCTTTTTTAGTTTTGACTAAAGTTAAAAATAGG
CACGAATAAAGATATCTAGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MENTVTELRFALDTFYFLISGVLVMWMAAGFAMLEAGLVRSKNTTEILTKNIVLYAIACTMFLLVGYNIMYVDNSAGGWL
PSFGSLIGIQAEGADHSLESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSVFLTGFIYPMEGYWTWGGGFLAEAGFS
DFAGSGIVHMAGAAAALAAVILLGARKGKFGKNGSVHPIPGSNMPLATLGTLILWMGWFGFNGGSQLALSNAENASAVGL
IFVNTNAAAATGAIAALIVSKLKWGKADLTMILNGALAGLVAITADPLSPTPLFSAGIGAVAGIIVVYSILTFDKLKLDD
PVGALSVHGVCGLFGLLVVPLNNEGASFATQLYGAAVIFGFVFVASWIVWSVIKVTMGVRVTAEEEETGMDLHDCGVEAY
PEFTAR

Sequences:

>Translated_406_residues
MENTVTELRFALDTFYFLISGVLVMWMAAGFAMLEAGLVRSKNTTEILTKNIVLYAIACTMFLLVGYNIMYVDNSAGGWL
PSFGSLIGIQAEGADHSLESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSVFLTGFIYPMEGYWTWGGGFLAEAGFS
DFAGSGIVHMAGAAAALAAVILLGARKGKFGKNGSVHPIPGSNMPLATLGTLILWMGWFGFNGGSQLALSNAENASAVGL
IFVNTNAAAATGAIAALIVSKLKWGKADLTMILNGALAGLVAITADPLSPTPLFSAGIGAVAGIIVVYSILTFDKLKLDD
PVGALSVHGVCGLFGLLVVPLNNEGASFATQLYGAAVIFGFVFVASWIVWSVIKVTMGVRVTAEEEETGMDLHDCGVEAY
PEFTAR
>Mature_406_residues
MENTVTELRFALDTFYFLISGVLVMWMAAGFAMLEAGLVRSKNTTEILTKNIVLYAIACTMFLLVGYNIMYVDNSAGGWL
PSFGSLIGIQAEGADHSLESDFFFQVVFVATAMSIVSGAVAERMKLWAFLIFSVFLTGFIYPMEGYWTWGGGFLAEAGFS
DFAGSGIVHMAGAAAALAAVILLGARKGKFGKNGSVHPIPGSNMPLATLGTLILWMGWFGFNGGSQLALSNAENASAVGL
IFVNTNAAAATGAIAALIVSKLKWGKADLTMILNGALAGLVAITADPLSPTPLFSAGIGAVAGIIVVYSILTFDKLKLDD
PVGALSVHGVCGLFGLLVVPLNNEGASFATQLYGAAVIFGFVFVASWIVWSVIKVTMGVRVTAEEEETGMDLHDCGVEAY
PEFTAR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=30.4136253041363, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=425, Percent_Identity=32.2352941176471, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=32.1596244131455, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI133931100, Length=434, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=28.7383177570093, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=28.8732394366197, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324187, Length=414, Percent_Identity=27.7777777777778, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321559, Length=420, Percent_Identity=27.3809523809524, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078314, Length=405, Percent_Identity=31.358024691358, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42719; Mature: 42719

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTVTELRFALDTFYFLISGVLVMWMAAGFAMLEAGLVRSKNTTEILTKNIVLYAIACT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MFLLVGYNIMYVDNSAGGWLPSFGSLIGIQAEGADHSLESDFFFQVVFVATAMSIVSGAV
HHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AERMKLWAFLIFSVFLTGFIYPMEGYWTWGGGFLAEAGFSDFAGSGIVHMAGAAAALAAV
HHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ILLGARKGKFGKNGSVHPIPGSNMPLATLGTLILWMGWFGFNGGSQLALSNAENASAVGL
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEE
IFVNTNAAAATGAIAALIVSKLKWGKADLTMILNGALAGLVAITADPLSPTPLFSAGIGA
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
VAGIIVVYSILTFDKLKLDDPVGALSVHGVCGLFGLLVVPLNNEGASFATQLYGAAVIFG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
FVFVASWIVWSVIKVTMGVRVTAEEEETGMDLHDCGVEAYPEFTAR
HHHHHHHHHHHHHHHHHCCEEEECHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENTVTELRFALDTFYFLISGVLVMWMAAGFAMLEAGLVRSKNTTEILTKNIVLYAIACT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MFLLVGYNIMYVDNSAGGWLPSFGSLIGIQAEGADHSLESDFFFQVVFVATAMSIVSGAV
HHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AERMKLWAFLIFSVFLTGFIYPMEGYWTWGGGFLAEAGFSDFAGSGIVHMAGAAAALAAV
HHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ILLGARKGKFGKNGSVHPIPGSNMPLATLGTLILWMGWFGFNGGSQLALSNAENASAVGL
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEE
IFVNTNAAAATGAIAALIVSKLKWGKADLTMILNGALAGLVAITADPLSPTPLFSAGIGA
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHH
VAGIIVVYSILTFDKLKLDDPVGALSVHGVCGLFGLLVVPLNNEGASFATQLYGAAVIFG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
FVFVASWIVWSVIKVTMGVRVTAEEEETGMDLHDCGVEAYPEFTAR
HHHHHHHHHHHHHHHHHCCEEEECHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]