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The map label for this gene is mlaE [H]

Identifier: 119946509

GI number: 119946509

Start: 3565151

End: 3565855

Strand: Reverse

Name: mlaE [H]

Synonym: Ping_2886

Alternate gene names: 119946509

Gene position: 3565855-3565151 (Counterclockwise)

Preceding gene: 119946510

Following gene: 119946508

Centisome position: 78.21

GC content: 43.12

Gene sequence:

>705_bases
ATGTTGTTCCATACAATGTTCGGCATGCCGCACCCTAAATTGTTTCCTTTATTTATCGGACAGATATTTCAACTTGGTAT
TTTATCTTTACCGATTATATTAACCGCTGGTTTGTTTATCGGCATGGTATTAAGCTTACAGGGTTATAATGTGCTGGTTG
ATTATGCTGCCGAGGAAAGTTTAGGCAGCATGGTGGCTTTATCGTTATTAAGAGAGTTAGGGCCCGTTGTAACGGCCTTA
CTTTTTGCCGGGCGAGCAGGCTCTGCGCTGACCGCTGAAATTGGTTTAATGCAAAGCACCGAGCAAATTGCCAGTATGGA
AATGATGGCAGTTTCCCCTCTTAAACGTGTGATAGCACCGCGTTTTCTTGCCGGCATCGTGACGCTGCCGTTGCTAACAT
TGATGTTTAATGTGGTGGCTATTTGGGGAGGATATTTAATCGGTGTCTCTTGGATGGGGGTTGATGGCGGAGCGTATTGG
TCTGTGATGCAGGGCAGTGTTGATTATCTGCCGGATGTTATGAATGGCGTGATTAAGAGTATTGTCTTTGGTGTAATGAT
TACCTGGGTAGCACTTTATAATGGTTATTATGTGATGCCAAACGCTATCGGTATTAGTCGCTCAACAACACAGACAGTAG
TCGTTTCATCATTATTAGTGTTAGCAATGGATTTTATATTAACGGTGTTTATGTTCGGAGCGTAA

Upstream 100 bases:

>100_bases
AATCTGATTTACAAAAGGTAAGTCGATAATGTTAGATTTTGCTGGCGCCGTCGGGCGTTTATCTTTATATTTTATAAAAA
CGACGGGAAAGTCCTGCGTA

Downstream 100 bases:

>100_bases
TCCGTCATATAACAAGTGGTTGTTTCATCACCATTATTAAATGTTAGCAATGGACTTTATATTAACGGTGTTTATGTTCG
GAGCGTAATCCGTCATATAA

Product: ABC-type transporter inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLFHTMFGMPHPKLFPLFIGQIFQLGILSLPIILTAGLFIGMVLSLQGYNVLVDYAAEESLGSMVALSLLRELGPVVTAL
LFAGRAGSALTAEIGLMQSTEQIASMEMMAVSPLKRVIAPRFLAGIVTLPLLTLMFNVVAIWGGYLIGVSWMGVDGGAYW
SVMQGSVDYLPDVMNGVIKSIVFGVMITWVALYNGYYVMPNAIGISRSTTQTVVVSSLLVLAMDFILTVFMFGA

Sequences:

>Translated_234_residues
MLFHTMFGMPHPKLFPLFIGQIFQLGILSLPIILTAGLFIGMVLSLQGYNVLVDYAAEESLGSMVALSLLRELGPVVTAL
LFAGRAGSALTAEIGLMQSTEQIASMEMMAVSPLKRVIAPRFLAGIVTLPLLTLMFNVVAIWGGYLIGVSWMGVDGGAYW
SVMQGSVDYLPDVMNGVIKSIVFGVMITWVALYNGYYVMPNAIGISRSTTQTVVVSSLLVLAMDFILTVFMFGA
>Mature_234_residues
MLFHTMFGMPHPKLFPLFIGQIFQLGILSLPIILTAGLFIGMVLSLQGYNVLVDYAAEESLGSMVALSLLRELGPVVTAL
LFAGRAGSALTAEIGLMQSTEQIASMEMMAVSPLKRVIAPRFLAGIVTLPLLTLMFNVVAIWGGYLIGVSWMGVDGGAYW
SVMQGSVDYLPDVMNGVIKSIVFGVMITWVALYNGYYVMPNAIGISRSTTQTVVVSSLLVLAMDFILTVFMFGA

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=234, Percent_Identity=61.965811965812, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 25203; Mature: 25203

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFHTMFGMPHPKLFPLFIGQIFQLGILSLPIILTAGLFIGMVLSLQGYNVLVDYAAEES
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
LGSMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMQSTEQIASMEMMAVSPLKRVIAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLAGIVTLPLLTLMFNVVAIWGGYLIGVSWMGVDGGAYWSVMQGSVDYLPDVMNGVIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHCCCHHHHHHHHHHHHHH
IVFGVMITWVALYNGYYVMPNAIGISRSTTQTVVVSSLLVLAMDFILTVFMFGA
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFHTMFGMPHPKLFPLFIGQIFQLGILSLPIILTAGLFIGMVLSLQGYNVLVDYAAEES
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
LGSMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMQSTEQIASMEMMAVSPLKRVIAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLAGIVTLPLLTLMFNVVAIWGGYLIGVSWMGVDGGAYWSVMQGSVDYLPDVMNGVIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHCCCHHHHHHHHHHHHHH
IVFGVMITWVALYNGYYVMPNAIGISRSTTQTVVVSSLLVLAMDFILTVFMFGA
HHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]