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The map label for this gene is rbsB [H]

Identifier: 119946432

GI number: 119946432

Start: 3452852

End: 3453793

Strand: Reverse

Name: rbsB [H]

Synonym: Ping_2806

Alternate gene names: 119946432

Gene position: 3453793-3452852 (Counterclockwise)

Preceding gene: 119946433

Following gene: 119946431

Centisome position: 75.75

GC content: 42.04

Gene sequence:

>942_bases
ATGAAAGGCTTAAAATATTTGGCAATGACTGGCGTGCTTTTTGCGGGAATCAGCACTTCTAGCTATGCAGGAGAACTCAA
TAAAATAGGTGTATCACTCGGCTCTATGGGCAATCCGTTTTTTGTGGCTTTAGCCAGCGGTGCTGAAGCTGAAGCAAAAA
AAATCAATCCTAACGTTGAAGTGCTGGCATTAGGTTATGATTACGATTTGGGTAAGCAGTTTAATCAGATTGATAACTTT
ATTGCAGCCGGTGTGGACTTAATCCTGTTAAATCCAGGCGATCCTAATGCGATTGAACCTGCGATTAAAAGAGCACAAAA
AGCGGGTATTGTTGTTGTCTCAGTTGATACGGCTGCTAAGGGTTCTGATGCCACAGTGACGACTGATAATGTCAGTGCCG
GTGCCGTAGCCTGTCAATATATTGTCGAAAAATTAGAGGGTAAAGGTAATGTTATTATTCAGAACGGACCGCAAGTGTCG
GCTGTTATCGATCGCGTGAATGGTTGTAAGGAAGTCTTAAAAGCAGCACCGGGAATCACCATTTTATCTGATGATCAAGA
CGGTAAAGGTTCTCGAGATGGTGGTATGTCTGTGATGTTAGGACATCTGACTCGTTTTAGTGAAATTGATGCCGTATTTG
CAATCAATGACCCGCAGGCAATTGGTGCAGATTTAGCGGCTAAGCAGCTTAAAAGAGATAATATAATTATTACTTCAGTT
GATGGCGCACCGGGTATTGTTGCTGCATTAAAAAATCCAGACAGCCCTATGATTCAAGCATCTGGTAGCCAAAATCCATT
TGTTATGGCATCAAAAGCAGTGGAGCTCGGATATCAAATAATGAATGGAGAGGTACCTGAAGAGAGTGTAATATTACTTC
CTTCTGAGTTGGTGACCCGCGATAATGTTAATGAATACAAAGGTTGGGATGCAGTTCGATAA

Upstream 100 bases:

>100_bases
CAGGGCAATACAAGAACATAAAAGATTAACTAACAGTTTACCCACGGGTCGCCATAAAACGGTGGCAACCCAACTACTTG
AATGAAAAATGGAGATTACA

Downstream 100 bases:

>100_bases
ATTAAAATTTGAGCCATGTTAACTAACATGGCTCTTACCTAATTTCTTATTCCATTTATTTTAAAAAAAAGCGAGTTATT
ACTTTCGCATGCAGCAGGAT

Product: transcriptional regulator, periplasmic binding protein of LacI family protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKGLKYLAMTGVLFAGISTSSYAGELNKIGVSLGSMGNPFFVALASGAEAEAKKINPNVEVLALGYDYDLGKQFNQIDNF
IAAGVDLILLNPGDPNAIEPAIKRAQKAGIVVVSVDTAAKGSDATVTTDNVSAGAVACQYIVEKLEGKGNVIIQNGPQVS
AVIDRVNGCKEVLKAAPGITILSDDQDGKGSRDGGMSVMLGHLTRFSEIDAVFAINDPQAIGADLAAKQLKRDNIIITSV
DGAPGIVAALKNPDSPMIQASGSQNPFVMASKAVELGYQIMNGEVPEESVILLPSELVTRDNVNEYKGWDAVR

Sequences:

>Translated_313_residues
MKGLKYLAMTGVLFAGISTSSYAGELNKIGVSLGSMGNPFFVALASGAEAEAKKINPNVEVLALGYDYDLGKQFNQIDNF
IAAGVDLILLNPGDPNAIEPAIKRAQKAGIVVVSVDTAAKGSDATVTTDNVSAGAVACQYIVEKLEGKGNVIIQNGPQVS
AVIDRVNGCKEVLKAAPGITILSDDQDGKGSRDGGMSVMLGHLTRFSEIDAVFAINDPQAIGADLAAKQLKRDNIIITSV
DGAPGIVAALKNPDSPMIQASGSQNPFVMASKAVELGYQIMNGEVPEESVILLPSELVTRDNVNEYKGWDAVR
>Mature_313_residues
MKGLKYLAMTGVLFAGISTSSYAGELNKIGVSLGSMGNPFFVALASGAEAEAKKINPNVEVLALGYDYDLGKQFNQIDNF
IAAGVDLILLNPGDPNAIEPAIKRAQKAGIVVVSVDTAAKGSDATVTTDNVSAGAVACQYIVEKLEGKGNVIIQNGPQVS
AVIDRVNGCKEVLKAAPGITILSDDQDGKGSRDGGMSVMLGHLTRFSEIDAVFAINDPQAIGADLAAKQLKRDNIIITSV
DGAPGIVAALKNPDSPMIQASGSQNPFVMASKAVELGYQIMNGEVPEESVILLPSELVTRDNVNEYKGWDAVR

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=287, Percent_Identity=29.616724738676, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790526, Length=278, Percent_Identity=29.8561151079137, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790674, Length=195, Percent_Identity=35.8974358974359, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32679; Mature: 32679

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKYLAMTGVLFAGISTSSYAGELNKIGVSLGSMGNPFFVALASGAEAEAKKINPNVE
CCCHHHHHHHHHHHHCCCCCHHCCCHHHHEEECCCCCCCEEEEEECCCCCHHHHCCCCEE
VLALGYDYDLGKQFNQIDNFIAAGVDLILLNPGDPNAIEPAIKRAQKAGIVVVSVDTAAK
EEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCC
GSDATVTTDNVSAGAVACQYIVEKLEGKGNVIIQNGPQVSAVIDRVNGCKEVLKAAPGIT
CCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEE
ILSDDQDGKGSRDGGMSVMLGHLTRFSEIDAVFAINDPQAIGADLAAKQLKRDNIIITSV
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEC
DGAPGIVAALKNPDSPMIQASGSQNPFVMASKAVELGYQIMNGEVPEESVILLPSELVTR
CCCCCEEEEECCCCCCEEEECCCCCCEEEEHHHHHHCHHEECCCCCCCCEEEECHHHHCC
DNVNEYKGWDAVR
CCCHHHCCCCCCC
>Mature Secondary Structure
MKGLKYLAMTGVLFAGISTSSYAGELNKIGVSLGSMGNPFFVALASGAEAEAKKINPNVE
CCCHHHHHHHHHHHHCCCCCHHCCCHHHHEEECCCCCCCEEEEEECCCCCHHHHCCCCEE
VLALGYDYDLGKQFNQIDNFIAAGVDLILLNPGDPNAIEPAIKRAQKAGIVVVSVDTAAK
EEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCC
GSDATVTTDNVSAGAVACQYIVEKLEGKGNVIIQNGPQVSAVIDRVNGCKEVLKAAPGIT
CCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEE
ILSDDQDGKGSRDGGMSVMLGHLTRFSEIDAVFAINDPQAIGADLAAKQLKRDNIIITSV
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEC
DGAPGIVAALKNPDSPMIQASGSQNPFVMASKAVELGYQIMNGEVPEESVILLPSELVTR
CCCCCEEEEECCCCCCEEEECCCCCCEEEEHHHHHHCHHEECCCCCCCCEEEECHHHHCC
DNVNEYKGWDAVR
CCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]