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The map label for this gene is pdhC [H]

Identifier: 119946406

GI number: 119946406

Start: 3420540

End: 3421835

Strand: Reverse

Name: pdhC [H]

Synonym: Ping_2780

Alternate gene names: 119946406

Gene position: 3421835-3420540 (Counterclockwise)

Preceding gene: 119946407

Following gene: 119946405

Centisome position: 75.05

GC content: 36.11

Gene sequence:

>1296_bases
ATGCCTATTGAAATAAAATTGCCTGAAGTAGTCAGTGGTTTCGAGAGTGGAGTCATTGCATCTTGGTGCGTTAATGAGGG
AGATAACATTAAAAAAGGTGATGTTATCTTTGAAGTGGAAACAGATAAAGCTGTTATTGAAGTTGAAAGTCCAGGGGCAG
GTGTTCTGGGTAAAATATTGGTTGATAGCAATAGCTCTCCTGTTGCTGTTGATACTATTGTTGGTATGATTTTATTAGAA
AATGAAGACCCCAGTGTACTTTCTGGTGAACCTGTCATTACTAATGATGATGCTAATACGCCTGCACCTGTAAGTGATGT
TAAGCCTGACAAGATTCAAGCAGTTCCTTCTGCATCTTCGGGCGCTAGCCGTATAATGGCAAGTCCATTAGCCAAGGTTA
TAGCAGCAAATAATAATATAGACTTGAGCAATGTTGTTGGGACAGGACCACGCAATAGAATTTTAAAAGCTGACGTCGAA
AATATTATTAATAATAAATCAGATAATTCACCTGCCATAATGACAACGAGTGCAGAAAATAAGCCTGATAATAGTGTTCC
TCTTGATAAGGTTGCCAGTACAGTAAATACTGAAAATAGTGATATTACGCCTCATACTGCAATGAGAAAAGTGATTGCTA
GCCGCCTAACTGAATCTAAAACTACGATACCGCATTTTTATGTGTCTATTGATTGTGAAGTTGATAACTTAAACTTATTA
AGAGCTGAGTTTAATGCTTTTTACAAAGATCACGAAAATGTGAAATTGACCGTTAATGATTTTATTATTAAGGCGGTTGC
ATTAGCTATCCATAAACACCCTGAAATTAACTCAATGTGGCTTAGTGAGGGTGTTAAGAAAAATAAAAACATCGATATTT
CAGTGGCTGTTTCTACTGACGATGGCCTTATGACTCCCATTGTATTTAATGCAGACAGAAAAGGTTTAATTACTCTTTCA
CAAAATATGAAGAGTCTAGTCAGTAAAACCCGAAGTGGTAAGTTACAACCCAATGAATATCAAGGGGGAGGATTTACTAT
TAGCAACCTGGGTATGTACGATATTGATTCTTTTAATGCCATCATTAACCCTCCACAATCTTGTATTTTGGCTGTTGGTC
GTGCCAAGAAAATACCTGTTGTGAAAGATGATCAAATTTTAATTGCCAATGTAATGAATTGTACTTTATCTGTAGATCAT
CGTGTTATAGATGGTTCAGTTGCTGCTGAGTTTTTACAAACATTTAAATTTTATATTGAAAACCCTAAACACATGATGCT
GTTTGGAGGCGAATAA

Upstream 100 bases:

>100_bases
TGAATTAGTGAGTTACCCTAATGGAAAAGGTTATGAAGCAGAATCTATTCATAAATTATGGCACCCAGCTTACCCTGCTG
AATAATAAAAAGGATTTATA

Downstream 100 bases:

>100_bases
GATGAGTTCTGCAGATATTGAATATGATGTCATTATTATAGGGGGTGGCCCCGGAGGTTATGTTAGTGCAATTAAAGCTG
CTCAAAACAACCTGAAAGTC

Product: dihydrolipoamide dehydrogenase E3 component of 3 enzyme complexes

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPIEIKLPEVVSGFESGVIASWCVNEGDNIKKGDVIFEVETDKAVIEVESPGAGVLGKILVDSNSSPVAVDTIVGMILLE
NEDPSVLSGEPVITNDDANTPAPVSDVKPDKIQAVPSASSGASRIMASPLAKVIAANNNIDLSNVVGTGPRNRILKADVE
NIINNKSDNSPAIMTTSAENKPDNSVPLDKVASTVNTENSDITPHTAMRKVIASRLTESKTTIPHFYVSIDCEVDNLNLL
RAEFNAFYKDHENVKLTVNDFIIKAVALAIHKHPEINSMWLSEGVKKNKNIDISVAVSTDDGLMTPIVFNADRKGLITLS
QNMKSLVSKTRSGKLQPNEYQGGGFTISNLGMYDIDSFNAIINPPQSCILAVGRAKKIPVVKDDQILIANVMNCTLSVDH
RVIDGSVAAEFLQTFKFYIENPKHMMLFGGE

Sequences:

>Translated_431_residues
MPIEIKLPEVVSGFESGVIASWCVNEGDNIKKGDVIFEVETDKAVIEVESPGAGVLGKILVDSNSSPVAVDTIVGMILLE
NEDPSVLSGEPVITNDDANTPAPVSDVKPDKIQAVPSASSGASRIMASPLAKVIAANNNIDLSNVVGTGPRNRILKADVE
NIINNKSDNSPAIMTTSAENKPDNSVPLDKVASTVNTENSDITPHTAMRKVIASRLTESKTTIPHFYVSIDCEVDNLNLL
RAEFNAFYKDHENVKLTVNDFIIKAVALAIHKHPEINSMWLSEGVKKNKNIDISVAVSTDDGLMTPIVFNADRKGLITLS
QNMKSLVSKTRSGKLQPNEYQGGGFTISNLGMYDIDSFNAIINPPQSCILAVGRAKKIPVVKDDQILIANVMNCTLSVDH
RVIDGSVAAEFLQTFKFYIENPKHMMLFGGE
>Mature_430_residues
PIEIKLPEVVSGFESGVIASWCVNEGDNIKKGDVIFEVETDKAVIEVESPGAGVLGKILVDSNSSPVAVDTIVGMILLEN
EDPSVLSGEPVITNDDANTPAPVSDVKPDKIQAVPSASSGASRIMASPLAKVIAANNNIDLSNVVGTGPRNRILKADVEN
IINNKSDNSPAIMTTSAENKPDNSVPLDKVASTVNTENSDITPHTAMRKVIASRLTESKTTIPHFYVSIDCEVDNLNLLR
AEFNAFYKDHENVKLTVNDFIIKAVALAIHKHPEINSMWLSEGVKKNKNIDISVAVSTDDGLMTPIVFNADRKGLITLSQ
NMKSLVSKTRSGKLQPNEYQGGGFTISNLGMYDIDSFNAIINPPQSCILAVGRAKKIPVVKDDQILIANVMNCTLSVDHR
VIDGSVAAEFLQTFKFYIENPKHMMLFGGE

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=38.8888888888889, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=36.0691144708423, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=36.0691144708423, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=46.583850931677, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=27.816091954023, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=31.6, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=28, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786305, Length=415, Percent_Identity=28.1927710843373, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=39.2935982339956, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17538894, Length=327, Percent_Identity=35.1681957186544, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=27.7136258660508, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=27.6849642004773, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=34.5982142857143, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6320352, Length=438, Percent_Identity=29.9086757990868, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24582497, Length=310, Percent_Identity=43.5483870967742, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI20129315, Length=310, Percent_Identity=43.5483870967742, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.4090909090909, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46495; Mature: 46364

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEIKLPEVVSGFESGVIASWCVNEGDNIKKGDVIFEVETDKAVIEVESPGAGVLGKIL
CCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEE
VDSNSSPVAVDTIVGMILLENEDPSVLSGEPVITNDDANTPAPVSDVKPDKIQAVPSASS
ECCCCCCEEHHHEEEEEEECCCCCCEECCCCEEECCCCCCCCCCCCCCCCCEEECCCCCC
GASRIMASPLAKVIAANNNIDLSNVVGTGPRNRILKADVENIINNKSDNSPAIMTTSAEN
CHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCEEEEECCCC
KPDNSVPLDKVASTVNTENSDITPHTAMRKVIASRLTESKTTIPHFYVSIDCEVDNLNLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEE
RAEFNAFYKDHENVKLTVNDFIIKAVALAIHKHPEINSMWLSEGVKKNKNIDISVAVSTD
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECC
DGLMTPIVFNADRKGLITLSQNMKSLVSKTRSGKLQPNEYQGGGFTISNLGMYDIDSFNA
CCCCCCEEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCCC
IINPPQSCILAVGRAKKIPVVKDDQILIANVMNCTLSVDHRVIDGSVAAEFLQTFKFYIE
CCCCCHHHHEECCCCCCCCEECCCCEEEEEEEEEEEEECCEEECHHHHHHHHHHHHHHHC
NPKHMMLFGGE
CCCEEEEECCC
>Mature Secondary Structure 
PIEIKLPEVVSGFESGVIASWCVNEGDNIKKGDVIFEVETDKAVIEVESPGAGVLGKIL
CEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEE
VDSNSSPVAVDTIVGMILLENEDPSVLSGEPVITNDDANTPAPVSDVKPDKIQAVPSASS
ECCCCCCEEHHHEEEEEEECCCCCCEECCCCEEECCCCCCCCCCCCCCCCCEEECCCCCC
GASRIMASPLAKVIAANNNIDLSNVVGTGPRNRILKADVENIINNKSDNSPAIMTTSAEN
CHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCEEEEECCCC
KPDNSVPLDKVASTVNTENSDITPHTAMRKVIASRLTESKTTIPHFYVSIDCEVDNLNLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEE
RAEFNAFYKDHENVKLTVNDFIIKAVALAIHKHPEINSMWLSEGVKKNKNIDISVAVSTD
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECC
DGLMTPIVFNADRKGLITLSQNMKSLVSKTRSGKLQPNEYQGGGFTISNLGMYDIDSFNA
CCCCCCEEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCCC
IINPPQSCILAVGRAKKIPVVKDDQILIANVMNCTLSVDHRVIDGSVAAEFLQTFKFYIE
CCCCCHHHHEECCCCCCCCEECCCCEEEEEEEEEEEEECCEEECHHHHHHHHHHHHHHHC
NPKHMMLFGGE
CCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA