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The map label for this gene is lhgO [H]

Identifier: 119946366

GI number: 119946366

Start: 3358917

End: 3360167

Strand: Direct

Name: lhgO [H]

Synonym: Ping_2738

Alternate gene names: 119946366

Gene position: 3358917-3360167 (Clockwise)

Preceding gene: 119946365

Following gene: 119946367

Centisome position: 73.67

GC content: 41.09

Gene sequence:

>1251_bases
ATGAAGGCAATGTATGATTATGTGATCATCGGCGGTGGTATTATTGGCGTATCAACAGCATGGCAACTGCAACAAAGATA
TCCTGAAAAATCGATTTTATTAGTAGAAAAAGAATCACAATTATCACGTCATCAAACCGGTCATAATAGTGGTGTTATCC
ATGCGGGTGTTTACTATAAACCAGGCAGTATGAAAGCGAAGTTCTGTAAAGCGGGCGTGAAAGCAACGACCGAATTTTGC
AAAAAACACAATATCCCGGTGGAAAATTGTGGCAAGTTACTGGTCGCAACCAACCAGCTCGAATTAGAGCGTATGCAGGA
TCTTTATCAACGCTGTCATGAGAACGAGATTCACGCAATACTATTAAACCAGGCAGAACTAAAAATAAGAGAACCCAATA
TTCAGGGACTTGGGGCCATTTTTGTCGACTCTACCAGCATAGTAAATTACACCCTGGTCACCCAAAAAATGGCAGAAGAA
TTTGTGAAACTTGGCGGTAAAGTGATGTTAGATACCAAGGTTATCAATTTAGTGGAGTCTGCTGAAAACACCATTTTAAC
CTGCCAAAGCAGCGCTGAAGAAGTGAGCATTACGAGTCGTTTTCTCGTCTCTTGCTCAGGCCTAATGGCTGACCGAATCA
CTAAAATGCTCAATATAGAGACCGACTTTCAAATTATTCCTTATCGTGGCGAATACCATCAATTGCCCGCAAAGCACAAT
AATATTGTCAATCATCTGATTTATCCGATTCCAGATCCCGAACTCCCTTTTCTTGGTGTGCATCTAACTCGAATGATTGA
TGGTTCTGTCACTGTGGGACCGAATGCGGTGCAGGGCTGGAAACGCGAAGGTTATGGTAAAATAAATATTAATTTCCGCG
ATATTCTGGATATGCTCAGTTTCTCAGGATTCTGGAAAGTCACCAAGAACAACTTAAAATCAGGATTAATTGAAACCAAA
AATTCATGGTGGAAACCAGGTTATTTGAAAATGGTCAATAAATACTGTGACCAGATTAAACTTGCTGACTTACTGCCTTA
TCCAGCCGGAATACGAGCTCAAGCAGTATTAAAAGATGGAAGCTTAGTTCATGACTTTCTCTTTGTAGAAAGCCCTCGCA
GCTTGCACGTTTGTAATGCACCCTCACCCGCAGCCACATCGGCAATCCCGATCGGCAATTATATTTGTGACAAAATAATG
GCAAAACAAGGCATTTCTAAGAGTGACGAATTATCCCAGAAATCATCTTAG

Upstream 100 bases:

>100_bases
ACAAAATCAAAAACGAAGCTAGCATGTGTTTCAACCAGCATAAATGTACAGAAAATTAATGGATTTGGTATTAATACTAA
TTCAGAAAAGGAAATAAAGA

Downstream 100 bases:

>100_bases
TTTAAATTAAAGCTTATCTTTCTGATTTAAAAGCAACCAGGGCATAACATCTAGTTCTGTTCACTTAAGATAAGCGGATT
TTTATTTCTGGCATCTCGTT

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKAMYDYVIIGGGIIGVSTAWQLQQRYPEKSILLVEKESQLSRHQTGHNSGVIHAGVYYKPGSMKAKFCKAGVKATTEFC
KKHNIPVENCGKLLVATNQLELERMQDLYQRCHENEIHAILLNQAELKIREPNIQGLGAIFVDSTSIVNYTLVTQKMAEE
FVKLGGKVMLDTKVINLVESAENTILTCQSSAEEVSITSRFLVSCSGLMADRITKMLNIETDFQIIPYRGEYHQLPAKHN
NIVNHLIYPIPDPELPFLGVHLTRMIDGSVTVGPNAVQGWKREGYGKININFRDILDMLSFSGFWKVTKNNLKSGLIETK
NSWWKPGYLKMVNKYCDQIKLADLLPYPAGIRAQAVLKDGSLVHDFLFVESPRSLHVCNAPSPAATSAIPIGNYICDKIM
AKQGISKSDELSQKSS

Sequences:

>Translated_416_residues
MKAMYDYVIIGGGIIGVSTAWQLQQRYPEKSILLVEKESQLSRHQTGHNSGVIHAGVYYKPGSMKAKFCKAGVKATTEFC
KKHNIPVENCGKLLVATNQLELERMQDLYQRCHENEIHAILLNQAELKIREPNIQGLGAIFVDSTSIVNYTLVTQKMAEE
FVKLGGKVMLDTKVINLVESAENTILTCQSSAEEVSITSRFLVSCSGLMADRITKMLNIETDFQIIPYRGEYHQLPAKHN
NIVNHLIYPIPDPELPFLGVHLTRMIDGSVTVGPNAVQGWKREGYGKININFRDILDMLSFSGFWKVTKNNLKSGLIETK
NSWWKPGYLKMVNKYCDQIKLADLLPYPAGIRAQAVLKDGSLVHDFLFVESPRSLHVCNAPSPAATSAIPIGNYICDKIM
AKQGISKSDELSQKSS
>Mature_416_residues
MKAMYDYVIIGGGIIGVSTAWQLQQRYPEKSILLVEKESQLSRHQTGHNSGVIHAGVYYKPGSMKAKFCKAGVKATTEFC
KKHNIPVENCGKLLVATNQLELERMQDLYQRCHENEIHAILLNQAELKIREPNIQGLGAIFVDSTSIVNYTLVTQKMAEE
FVKLGGKVMLDTKVINLVESAENTILTCQSSAEEVSITSRFLVSCSGLMADRITKMLNIETDFQIIPYRGEYHQLPAKHN
NIVNHLIYPIPDPELPFLGVHLTRMIDGSVTVGPNAVQGWKREGYGKININFRDILDMLSFSGFWKVTKNNLKSGLIETK
NSWWKPGYLKMVNKYCDQIKLADLLPYPAGIRAQAVLKDGSLVHDFLFVESPRSLHVCNAPSPAATSAIPIGNYICDKIM
AKQGISKSDELSQKSS

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=402, Percent_Identity=38.8059701492537, Blast_Score=295, Evalue=7e-80,
Organism=Escherichia coli, GI87082148, Length=396, Percent_Identity=58.3333333333333, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17565764, Length=405, Percent_Identity=39.0123456790123, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24585081, Length=401, Percent_Identity=37.6558603491272, Blast_Score=285, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 46433; Mature: 46433

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMYDYVIIGGGIIGVSTAWQLQQRYPEKSILLVEKESQLSRHQTGHNSGVIHAGVYYK
CCCEEEEEEECCCEEECCHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCEEEEEEEEC
PGSMKAKFCKAGVKATTEFCKKHNIPVENCGKLLVATNQLELERMQDLYQRCHENEIHAI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECHHHHHHHHHHHHHHCCCCEEEE
LLNQAELKIREPNIQGLGAIFVDSTSIVNYTLVTQKMAEEFVKLGGKVMLDTKVINLVES
EEECCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
AENTILTCQSSAEEVSITSRFLVSCSGLMADRITKMLNIETDFQIIPYRGEYHQLPAKHN
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCHHC
NIVNHLIYPIPDPELPFLGVHLTRMIDGSVTVGPNAVQGWKREGYGKININFRDILDMLS
CCHHHEEECCCCCCCCCHHHHHHHHCCCCEEECCHHHHCHHHCCCCEEEEEHHHHHHHHH
FSGFWKVTKNNLKSGLIETKNSWWKPGYLKMVNKYCDQIKLADLLPYPAGIRAQAVLKDG
CCCEEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCEEEEECCC
SLVHDFLFVESPRSLHVCNAPSPAATSAIPIGNYICDKIMAKQGISKSDELSQKSS
CEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MKAMYDYVIIGGGIIGVSTAWQLQQRYPEKSILLVEKESQLSRHQTGHNSGVIHAGVYYK
CCCEEEEEEECCCEEECCHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCEEEEEEEEC
PGSMKAKFCKAGVKATTEFCKKHNIPVENCGKLLVATNQLELERMQDLYQRCHENEIHAI
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECHHHHHHHHHHHHHHCCCCEEEE
LLNQAELKIREPNIQGLGAIFVDSTSIVNYTLVTQKMAEEFVKLGGKVMLDTKVINLVES
EEECCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
AENTILTCQSSAEEVSITSRFLVSCSGLMADRITKMLNIETDFQIIPYRGEYHQLPAKHN
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCHHC
NIVNHLIYPIPDPELPFLGVHLTRMIDGSVTVGPNAVQGWKREGYGKININFRDILDMLS
CCHHHEEECCCCCCCCCHHHHHHHHCCCCEEECCHHHHCHHHCCCCEEEEEHHHHHHHHH
FSGFWKVTKNNLKSGLIETKNSWWKPGYLKMVNKYCDQIKLADLLPYPAGIRAQAVLKDG
CCCEEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCEEEEECCC
SLVHDFLFVESPRSLHVCNAPSPAATSAIPIGNYICDKIMAKQGISKSDELSQKSS
CEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]