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The map label for this gene is cyoE

Identifier: 119946339

GI number: 119946339

Start: 3325916

End: 3326851

Strand: Reverse

Name: cyoE

Synonym: Ping_2710

Alternate gene names: 119946339

Gene position: 3326851-3325916 (Counterclockwise)

Preceding gene: 119946340

Following gene: 119946338

Centisome position: 72.96

GC content: 42.95

Gene sequence:

>936_bases
ATGGCGAATAGTATCACTCACAATACAGCAACGGTCAGCGCCTCATTAAATGAGGACTCCTTAACTGAGAGTCGCTTGCC
TACAATAAATGACTACTTAGAGTTGTGCAAAATCAAAGTGGTGGCGTTACTGGTCTTAACCGCATTGGTTGGATTAGCGC
TGGCGCCCGATATGGGGCGAGGTTATTTCGTGCAGCTGTTAAGTTTGTTCGGAATCGGTTTATTATCGAGCAGTGCCGCT
GTGATTAATCATATCGTAGACAGCAAAATTGACGCAAAAATGGTGCGCACAAAAAACCGACCGTTAGTGAGAAAAGCCGT
GTCTCGCAGGCAAGCCTTGATCTTTTCAGCTGTAATAGGGACATTAGGTTTTAGCTTGCTGGTCTTTGCCGCAAATTGGT
TAACGGCGCAAATGACGCTGTTTGCGCTGGTGGGTTATGCATTTGTCTACACGATGTTTTTAAAACGAGCGACACCACAA
AATATTGTGATCGGCGGATTAGCGGGTGCGATGCCTCCTCTTCTGGGTTGGATTTCTGAAACCGGACAATTAGCCGCACA
GCCGTGGATCTTAGTGATGATCATATTCACCTGGACGCCTCCCCATTTTTGGGCGCTGGCGATTCACCGTAAAGCCGATT
ATGCAAAAGCTAAAATACCCATGTTGCCGGTCACGCACGGCACTGATTTCACCAAAACCTGTATATTGTTATATAGCTTA
TTATTGACCGTGGTGTGTTTTTTACCTTTCTTAATCCATATGTCGGGTTATTTGTATTTATTCGTCGCCATGCTGATCAA
TATTATTTTTATCTATAAAGCCGTCGCATTAAAATTATCGGAAACATCCTATGGTGCGTTGAATCTTTTTAAATATTCGA
TTCTCCATTTAACTCTGTTATTCATAGCCTTATTTGCAGATAAATTTCTGATGTAA

Upstream 100 bases:

>100_bases
TGTTACTGCTGAGTTTAATAAGATTAACCTATTACGTGAAATTATTAGCATTGCCGCATAAGTCGATTGCCGGTTTTAAT
ACTTTTTAAGGAGAAAAATA

Downstream 100 bases:

>100_bases
TAATAAACCGACCTTTGCGCGTTTTTTCCCCGATCCCTGCTTGATAATGCTATTATCTCCGGCTTGTCATTATTATTTTA
AGCAGCTTGAAGGTCAATAT

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MANSITHNTATVSASLNEDSLTESRLPTINDYLELCKIKVVALLVLTALVGLALAPDMGRGYFVQLLSLFGIGLLSSSAA
VINHIVDSKIDAKMVRTKNRPLVRKAVSRRQALIFSAVIGTLGFSLLVFAANWLTAQMTLFALVGYAFVYTMFLKRATPQ
NIVIGGLAGAMPPLLGWISETGQLAAQPWILVMIIFTWTPPHFWALAIHRKADYAKAKIPMLPVTHGTDFTKTCILLYSL
LLTVVCFLPFLIHMSGYLYLFVAMLINIIFIYKAVALKLSETSYGALNLFKYSILHLTLLFIALFADKFLM

Sequences:

>Translated_311_residues
MANSITHNTATVSASLNEDSLTESRLPTINDYLELCKIKVVALLVLTALVGLALAPDMGRGYFVQLLSLFGIGLLSSSAA
VINHIVDSKIDAKMVRTKNRPLVRKAVSRRQALIFSAVIGTLGFSLLVFAANWLTAQMTLFALVGYAFVYTMFLKRATPQ
NIVIGGLAGAMPPLLGWISETGQLAAQPWILVMIIFTWTPPHFWALAIHRKADYAKAKIPMLPVTHGTDFTKTCILLYSL
LLTVVCFLPFLIHMSGYLYLFVAMLINIIFIYKAVALKLSETSYGALNLFKYSILHLTLLFIALFADKFLM
>Mature_310_residues
ANSITHNTATVSASLNEDSLTESRLPTINDYLELCKIKVVALLVLTALVGLALAPDMGRGYFVQLLSLFGIGLLSSSAAV
INHIVDSKIDAKMVRTKNRPLVRKAVSRRQALIFSAVIGTLGFSLLVFAANWLTAQMTLFALVGYAFVYTMFLKRATPQN
IVIGGLAGAMPPLLGWISETGQLAAQPWILVMIIFTWTPPHFWALAIHRKADYAKAKIPMLPVTHGTDFTKTCILLYSLL
LTVVCFLPFLIHMSGYLYLFVAMLINIIFIYKAVALKLSETSYGALNLFKYSILHLTLLFIALFADKFLM

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=196, Percent_Identity=39.2857142857143, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1786631, Length=279, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71998235, Length=264, Percent_Identity=31.0606060606061, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325085, Length=264, Percent_Identity=32.5757575757576, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921064, Length=281, Percent_Identity=31.3167259786477, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYOE_PSYIN (A1SY55)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944019.1
- STRING:   A1SY55
- GeneID:   4625889
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2710
- eggNOG:   COG0109
- HOGENOM:   HBG656003
- OMA:   VPFDSQM
- PhylomeDB:   A1SY55
- ProtClustDB:   CLSK769536
- BioCyc:   PING357804:PING_2710-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.- [C]

Molecular weight: Translated: 34326; Mature: 34194

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17aeee34)-; HASH(0x1a0d39e0)-; HASH(0x19f03604)-; HASH(0x19841190)-; HASH(0x19ba481c)-; HASH(0x1a0d0f24)-; HASH(0x18ec9110)-; HASH(0x1a0d1014)-; HASH(0x1a0d5f1c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSITHNTATVSASLNEDSLTESRLPTINDYLELCKIKVVALLVLTALVGLALAPDMGR
CCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GYFVQLLSLFGIGLLSSSAAVINHIVDSKIDAKMVRTKNRPLVRKAVSRRQALIFSAVIG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TLGFSLLVFAANWLTAQMTLFALVGYAFVYTMFLKRATPQNIVIGGLAGAMPPLLGWISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHH
TGQLAAQPWILVMIIFTWTPPHFWALAIHRKADYAKAKIPMLPVTHGTDFTKTCILLYSL
CCHHHCCCHHHEEHHHHCCCCHHHHHHHHHHCCHHHHCCCCEECCCCCCHHHHHHHHHHH
LLTVVCFLPFLIHMSGYLYLFVAMLINIIFIYKAVALKLSETSYGALNLFKYSILHLTLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FIALFADKFLM
HHHHHHHHHCH
>Mature Secondary Structure 
ANSITHNTATVSASLNEDSLTESRLPTINDYLELCKIKVVALLVLTALVGLALAPDMGR
CCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC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HHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA