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The map label for this gene is radA [H]

Identifier: 119946267

GI number: 119946267

Start: 3225814

End: 3227226

Strand: Direct

Name: radA [H]

Synonym: Ping_2630

Alternate gene names: 119946267

Gene position: 3225814-3227226 (Clockwise)

Preceding gene: 119946266

Following gene: 119946273

Centisome position: 70.75

GC content: 41.97

Gene sequence:

>1413_bases
ATGGCGAAAGCAAAAACAAAAATCGCATATGTCTGCAGTGACTGTGGTGCGAATTACCAAAAATGGCAAGGCCAATGCGG
CGAATGTAAAGCATGGAATACCATTACAGAATTCAGAGAAGCATCGAGTACAACAAAATCTGTGAATGCGGCTGTCACTC
GGAATAAAAATGCGGGTTACACGGGCGCGTTAAGCAATGGTGTACAACGAATTTCGGGCGTGCAGATTGAAAATGCAACA
CGGGTCTCAACCGGACTTTCAGAATTGGATCGCGTTTTGGGCGGGGGCATTACCCTCGGCTCAGTGGTCCTTATCAGTGG
CGATCCGGGTTCAGGTAAAACAACCTTACTTACCAAGGTAGCAGAAGTCATGTCACAGAGCATGTCAACACTCTATGTCA
CCGCAGAAGAAAGCCTTAGCCAATGGGCTAAACGCGGTATTGAACGCTTAAAACTCAATTTTAACGAAGATAATTTTTTA
TTATCGGACACCGACTGCATTGAAGATATCGTCGATCAGTGTCTGGAAAATAATGTTCGTTTTTTGATTGCCGATTCAAT
TCAAGCTTTTGAATCAAATCAGGCGGAAGGCACCGCAGGTGGGGTCACTCAGGTAAAAATTTGTGCCAAAATTTTAAACC
GATTATGTAAACAACATGGTATTACGTTAATTCTTGTCGGTCAGGTAAATAAAAACTCGCAAATGGCCGGACCACAAACC
TTAAAACACATTATTGATACTGCTATCCACATCGAAGTCAATGATGCATCGGTGCGTATATTACGCGCCGATAAAAACCG
TTTTGGAGATACTGAACAGGTCGGTATTTTTCAGATGACAGAAAAGGGCATGTTTTCGGTTGATAACCCAAGTCGTCTTT
TCCTCTCAGGCAGCGAAGAACATTTCGAAGGATCAGCTATCTCGGTGATTAAAGATGGCGCCCGCAATTTACTGATTGAA
ATACAAGCTCTGGCCACCGAAGTTGAAGGCGAAAAATCTATTCGTAACTGTATTGGTATTTCCTACAGTCGTTTATCCTT
AATCACCGCAGTATTAAAAAAACACGGCGGTATTCGTACCTATTATGATATTAATATTAGTTTAGTTGGCGGTCTTAAAA
TGTCCGATTCAGAAACCTCAACGGATATGGCGGTGATGGCCGCATTATTAAGCTCAATTAATTCCAAACCTGTCCCTCAA
GATTCGGTATTTATTGGGGAGATAGCCCTGACCGGTGAAATACGTCAAATACCGCAAATTGTGCCGCGTGTACGCGAAGC
CTTGAGCCACGGTAAAAAACAGATCTATATTCCCGCAGTGGCTTATCATAAATCGATGCAACAGTTTGTCAAAAAAGGAC
AATCCATTCATCCACTTAAAAATATTAAAGAATTAATTGAAGCATTAAGTTAA

Upstream 100 bases:

>100_bases
CGGCTGACGGCTGACGGCTATCAGCTGACGGCTATCGGCTTGGAGCCTTACAGCTTTAAACCCTTTCAGTATTAACGAAT
TTTTCAATGGATTATAAATA

Downstream 100 bases:

>100_bases
AGCGTTAAGGACAAAAGGCGTGAAGGCTGTAAAGCTATAAGGCTCGAAGCTAAAAGACTCGAAGCTAAAAGCTTTTTTAA
CCGTATATATGTCAAAATTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAKAKTKIAYVCSDCGANYQKWQGQCGECKAWNTITEFREASSTTKSVNAAVTRNKNAGYTGALSNGVQRISGVQIENAT
RVSTGLSELDRVLGGGITLGSVVLISGDPGSGKTTLLTKVAEVMSQSMSTLYVTAEESLSQWAKRGIERLKLNFNEDNFL
LSDTDCIEDIVDQCLENNVRFLIADSIQAFESNQAEGTAGGVTQVKICAKILNRLCKQHGITLILVGQVNKNSQMAGPQT
LKHIIDTAIHIEVNDASVRILRADKNRFGDTEQVGIFQMTEKGMFSVDNPSRLFLSGSEEHFEGSAISVIKDGARNLLIE
IQALATEVEGEKSIRNCIGISYSRLSLITAVLKKHGGIRTYYDINISLVGGLKMSDSETSTDMAVMAALLSSINSKPVPQ
DSVFIGEIALTGEIRQIPQIVPRVREALSHGKKQIYIPAVAYHKSMQQFVKKGQSIHPLKNIKELIEALS

Sequences:

>Translated_470_residues
MAKAKTKIAYVCSDCGANYQKWQGQCGECKAWNTITEFREASSTTKSVNAAVTRNKNAGYTGALSNGVQRISGVQIENAT
RVSTGLSELDRVLGGGITLGSVVLISGDPGSGKTTLLTKVAEVMSQSMSTLYVTAEESLSQWAKRGIERLKLNFNEDNFL
LSDTDCIEDIVDQCLENNVRFLIADSIQAFESNQAEGTAGGVTQVKICAKILNRLCKQHGITLILVGQVNKNSQMAGPQT
LKHIIDTAIHIEVNDASVRILRADKNRFGDTEQVGIFQMTEKGMFSVDNPSRLFLSGSEEHFEGSAISVIKDGARNLLIE
IQALATEVEGEKSIRNCIGISYSRLSLITAVLKKHGGIRTYYDINISLVGGLKMSDSETSTDMAVMAALLSSINSKPVPQ
DSVFIGEIALTGEIRQIPQIVPRVREALSHGKKQIYIPAVAYHKSMQQFVKKGQSIHPLKNIKELIEALS
>Mature_469_residues
AKAKTKIAYVCSDCGANYQKWQGQCGECKAWNTITEFREASSTTKSVNAAVTRNKNAGYTGALSNGVQRISGVQIENATR
VSTGLSELDRVLGGGITLGSVVLISGDPGSGKTTLLTKVAEVMSQSMSTLYVTAEESLSQWAKRGIERLKLNFNEDNFLL
SDTDCIEDIVDQCLENNVRFLIADSIQAFESNQAEGTAGGVTQVKICAKILNRLCKQHGITLILVGQVNKNSQMAGPQTL
KHIIDTAIHIEVNDASVRILRADKNRFGDTEQVGIFQMTEKGMFSVDNPSRLFLSGSEEHFEGSAISVIKDGARNLLIEI
QALATEVEGEKSIRNCIGISYSRLSLITAVLKKHGGIRTYYDINISLVGGLKMSDSETSTDMAVMAALLSSINSKPVPQD
SVFIGEIALTGEIRQIPQIVPRVREALSHGKKQIYIPAVAYHKSMQQFVKKGQSIHPLKNIKELIEALS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=475, Percent_Identity=41.2631578947368, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 51076; Mature: 50945

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKTKIAYVCSDCGANYQKWQGQCGECKAWNTITEFREASSTTKSVNAAVTRNKNAGY
CCCCCCHHEEEEHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHEEECCCCCC
TGALSNGVQRISGVQIENATRVSTGLSELDRVLGGGITLGSVVLISGDPGSGKTTLLTKV
CCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCEEECEEEEEECCCCCCCHHHHHHH
AEVMSQSMSTLYVTAEESLSQWAKRGIERLKLNFNEDNFLLSDTDCIEDIVDQCLENNVR
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHEEEECCCCCEEECCHHHHHHHHHHHHHCCCE
FLIADSIQAFESNQAEGTAGGVTQVKICAKILNRLCKQHGITLILVGQVNKNSQMAGPQT
EEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHH
LKHIIDTAIHIEVNDASVRILRADKNRFGDTEQVGIFQMTEKGMFSVDNPSRLFLSGSEE
HHHHHCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCEECCCCCEEEEECCHH
HFEGSAISVIKDGARNLLIEIQALATEVEGEKSIRNCIGISYSRLSLITAVLKKHGGIRT
HCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
YYDINISLVGGLKMSDSETSTDMAVMAALLSSINSKPVPQDSVFIGEIALTGEIRQIPQI
EEEEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHH
VPRVREALSHGKKQIYIPAVAYHKSMQQFVKKGQSIHPLKNIKELIEALS
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
AKAKTKIAYVCSDCGANYQKWQGQCGECKAWNTITEFREASSTTKSVNAAVTRNKNAGY
CCCCCHHEEEEHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHEEECCCCCC
TGALSNGVQRISGVQIENATRVSTGLSELDRVLGGGITLGSVVLISGDPGSGKTTLLTKV
CCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCEEECEEEEEECCCCCCCHHHHHHH
AEVMSQSMSTLYVTAEESLSQWAKRGIERLKLNFNEDNFLLSDTDCIEDIVDQCLENNVR
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHEEEECCCCCEEECCHHHHHHHHHHHHHCCCE
FLIADSIQAFESNQAEGTAGGVTQVKICAKILNRLCKQHGITLILVGQVNKNSQMAGPQT
EEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHH
LKHIIDTAIHIEVNDASVRILRADKNRFGDTEQVGIFQMTEKGMFSVDNPSRLFLSGSEE
HHHHHCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCEECCCCCEEEEECCHH
HFEGSAISVIKDGARNLLIEIQALATEVEGEKSIRNCIGISYSRLSLITAVLKKHGGIRT
HCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
YYDINISLVGGLKMSDSETSTDMAVMAALLSSINSKPVPQDSVFIGEIALTGEIRQIPQI
EEEEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHH
VPRVREALSHGKKQIYIPAVAYHKSMQQFVKKGQSIHPLKNIKELIEALS
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]