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The map label for this gene is phoA [H]

Identifier: 119946264

GI number: 119946264

Start: 3222230

End: 3223822

Strand: Reverse

Name: phoA [H]

Synonym: Ping_2627

Alternate gene names: 119946264

Gene position: 3223822-3222230 (Counterclockwise)

Preceding gene: 119946265

Following gene: 119946261

Centisome position: 70.7

GC content: 42.0

Gene sequence:

>1593_bases
ATGACAAACTTAAACACAGTTAAAAAAACGTTTTTAGCGCTTTCTATTACTCTTGCGAGTTGTTCAAGCTTTGCAGAAAC
ACCCGTTAAAAATGTTATTTTAATGATTGGTGATGGTATGGGCCCCGGACAAATGGGACTTTTAGAAGAATATGCCCGTA
AAGCCCCGCATTCTATTTATAATGGCCGTGATACTGCACTTAAAACGATGATAGATAATGGTGTCTTAGGTATGTCACTA
CACAGCCCAGCGGATGCTTTAGTAGTGGATTCAGCTTGTTCGGCTTCGCAGTTGGCATCGGGTGTGAGTGCACCTTCTGA
AGCCTTAGGCATTGATGTTGATGGTAATCCTGTTGAAAGCGTATTAGAAATCGCTAAAAGTTTAGGTAAAGCGACGGGAC
TTGTTTCCGATACTCGTTTAACTCATGCAACTCCCGCTGCATTTGCATCCCATCAACCACACCGTTCATTGGAAAATCAG
ATAGCTGAAGAGATGTTGGCAACCCGTGTTGATGTGATGTTGTCGGGTGGGCTGCGTTACTGGATACCGATGTCCGCGAA
TGAGGGCGGCGCTGTATATCAAAAACTGCTTAAAAAAACGGGTGGTGATATTAAAATTAAATCTAAACGTATCGATGAAA
AAAACCTATTAACAGAAGCTGAAGGTGCAGGTTACGCCGTCGTCCATACGCGTGATGCACTTGCAAACGCGCCGGGCGAT
CAGATTCTTGGTTTATTTGCATACTCCGGTATGCAGGATGGTATTACCTATAGCAATACTAAAAACTCGCCAGAGCGTTC
CATGCCTAGCCTGAAAGAAATGACCATAAAAGCGCTGGATGTGTTATCCCAAGATCCCGATGGTTTCTTTTTAATGGTTG
AAGGGGGGCAGATTGATTGGGCCGGACATAATAACGATGCGGCAACCATGTTACATGAAATGCTTAAATTTGATGAAACA
GTCGAATATGTTTATGAATGGGTAAAAAACCGGAAAGATACCTTAGTCGTTGTTACGGCTGACCATGAAACGGGTGGATT
TGGTTTTAGTTATTCACGTGCTAATACACCAAAAGCTGAAAAACGAACCGGTATCGCTTTTAAAGATCGTGACTACAAGC
CGAATTTCAACTTTGGTAACCTCGCTATTTTAGACAAGTTATATGAGCAGAAAAAAAGTTTTGGCAATATGCTGGCTGAA
TTTGATCAAACAGATAAATCGCCTGAATCATTGCTGGCAATAGTGAATGCCAATAGTGAATTTAAGATAAGTCTGGCGCA
GGCAAAAGAGATATTAGTGACCGAAGAAAATGAATTTTACGATCCTGAGAACAAATACTTAAAACTTAAAACCTTTCCTA
AAGTTAATGATTTTAAAGCCTTTTATGTTTATGGTGAAGAAGTCCGCAATGATTTAATTGGCCGAGCGATGGGCGAAGAG
CAGAATATAGTTTGGGGTACTGGCACGCATACGGATACACCCGTTGCAGTGATTGCTTATGGACCCGATCAGGCTACCGC
GCCATTTTCGTCTCTTACCCACCATACGGAAATTGGGAAGTTACTTATTAAGACGGTACGGTCTGGACACTAA

Upstream 100 bases:

>100_bases
GCTAATATGATTATGATTTTATTTAAATCGTGATTGTCATAAAAATGTCATACATCTCCTTTATAGTCGCCGCCAATAAA
TAATACAAAGGACAATATTG

Downstream 100 bases:

>100_bases
CGGATTCCATTTTCTTTTTTAATCTGAACAGAGATAAAAAACCAGGGTGAATAATTCACTCTGGTTTTTTAGTCTCTAAC
GCATTAATGGAGAGGTAAAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTNLNTVKKTFLALSITLASCSSFAETPVKNVILMIGDGMGPGQMGLLEEYARKAPHSIYNGRDTALKTMIDNGVLGMSL
HSPADALVVDSACSASQLASGVSAPSEALGIDVDGNPVESVLEIAKSLGKATGLVSDTRLTHATPAAFASHQPHRSLENQ
IAEEMLATRVDVMLSGGLRYWIPMSANEGGAVYQKLLKKTGGDIKIKSKRIDEKNLLTEAEGAGYAVVHTRDALANAPGD
QILGLFAYSGMQDGITYSNTKNSPERSMPSLKEMTIKALDVLSQDPDGFFLMVEGGQIDWAGHNNDAATMLHEMLKFDET
VEYVYEWVKNRKDTLVVVTADHETGGFGFSYSRANTPKAEKRTGIAFKDRDYKPNFNFGNLAILDKLYEQKKSFGNMLAE
FDQTDKSPESLLAIVNANSEFKISLAQAKEILVTEENEFYDPENKYLKLKTFPKVNDFKAFYVYGEEVRNDLIGRAMGEE
QNIVWGTGTHTDTPVAVIAYGPDQATAPFSSLTHHTEIGKLLIKTVRSGH

Sequences:

>Translated_530_residues
MTNLNTVKKTFLALSITLASCSSFAETPVKNVILMIGDGMGPGQMGLLEEYARKAPHSIYNGRDTALKTMIDNGVLGMSL
HSPADALVVDSACSASQLASGVSAPSEALGIDVDGNPVESVLEIAKSLGKATGLVSDTRLTHATPAAFASHQPHRSLENQ
IAEEMLATRVDVMLSGGLRYWIPMSANEGGAVYQKLLKKTGGDIKIKSKRIDEKNLLTEAEGAGYAVVHTRDALANAPGD
QILGLFAYSGMQDGITYSNTKNSPERSMPSLKEMTIKALDVLSQDPDGFFLMVEGGQIDWAGHNNDAATMLHEMLKFDET
VEYVYEWVKNRKDTLVVVTADHETGGFGFSYSRANTPKAEKRTGIAFKDRDYKPNFNFGNLAILDKLYEQKKSFGNMLAE
FDQTDKSPESLLAIVNANSEFKISLAQAKEILVTEENEFYDPENKYLKLKTFPKVNDFKAFYVYGEEVRNDLIGRAMGEE
QNIVWGTGTHTDTPVAVIAYGPDQATAPFSSLTHHTEIGKLLIKTVRSGH
>Mature_529_residues
TNLNTVKKTFLALSITLASCSSFAETPVKNVILMIGDGMGPGQMGLLEEYARKAPHSIYNGRDTALKTMIDNGVLGMSLH
SPADALVVDSACSASQLASGVSAPSEALGIDVDGNPVESVLEIAKSLGKATGLVSDTRLTHATPAAFASHQPHRSLENQI
AEEMLATRVDVMLSGGLRYWIPMSANEGGAVYQKLLKKTGGDIKIKSKRIDEKNLLTEAEGAGYAVVHTRDALANAPGDQ
ILGLFAYSGMQDGITYSNTKNSPERSMPSLKEMTIKALDVLSQDPDGFFLMVEGGQIDWAGHNNDAATMLHEMLKFDETV
EYVYEWVKNRKDTLVVVTADHETGGFGFSYSRANTPKAEKRTGIAFKDRDYKPNFNFGNLAILDKLYEQKKSFGNMLAEF
DQTDKSPESLLAIVNANSEFKISLAQAKEILVTEENEFYDPENKYLKLKTFPKVNDFKAFYVYGEEVRNDLIGRAMGEEQ
NIVWGTGTHTDTPVAVIAYGPDQATAPFSSLTHHTEIGKLLIKTVRSGH

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=385, Percent_Identity=29.8701298701299, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157266292, Length=376, Percent_Identity=31.6489361702128, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157266296, Length=349, Percent_Identity=30.945558739255, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI294660770, Length=381, Percent_Identity=29.1338582677165, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI294660772, Length=330, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI94721246, Length=349, Percent_Identity=30.3724928366762, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI48994877, Length=367, Percent_Identity=32.425068119891, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=362, Percent_Identity=34.5303867403315, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24585213, Length=360, Percent_Identity=32.5, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21355151, Length=351, Percent_Identity=29.9145299145299, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355981, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24651554, Length=350, Percent_Identity=32.2857142857143, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21358067, Length=365, Percent_Identity=30.4109589041096, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859923, Length=377, Percent_Identity=29.1777188328912, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651556, Length=303, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19921912, Length=377, Percent_Identity=29.973474801061, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21355149, Length=376, Percent_Identity=26.3297872340426, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24657827, Length=349, Percent_Identity=28.6532951289398, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI85725200, Length=353, Percent_Identity=26.3456090651558, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI85815833, Length=353, Percent_Identity=26.3456090651558, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24657835, Length=351, Percent_Identity=27.3504273504274, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24657842, Length=352, Percent_Identity=26.4204545454545, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 57814; Mature: 57683

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNTVKKTFLALSITLASCSSFAETPVKNVILMIGDGMGPGQMGLLEEYARKAPHSIY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHC
NGRDTALKTMIDNGVLGMSLHSPADALVVDSACSASQLASGVSAPSEALGIDVDGNPVES
CCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHHHHHHCCCCCHHHEEECCCCCHHHH
VLEIAKSLGKATGLVSDTRLTHATPAAFASHQPHRSLENQIAEEMLATRVDVMLSGGLRY
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCEEE
WIPMSANEGGAVYQKLLKKTGGDIKIKSKRIDEKNLLTEAEGAGYAVVHTRDALANAPGD
EEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEHHHHCCCCCH
QILGLFAYSGMQDGITYSNTKNSPERSMPSLKEMTIKALDVLSQDPDGFFLMVEGGQIDW
HHHHHHHHCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
AGHNNDAATMLHEMLKFDETVEYVYEWVKNRKDTLVVVTADHETGGFGFSYSRANTPKAE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHH
KRTGIAFKDRDYKPNFNFGNLAILDKLYEQKKSFGNMLAEFDQTDKSPESLLAIVNANSE
HHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
FKISLAQAKEILVTEENEFYDPENKYLKLKTFPKVNDFKAFYVYGEEVRNDLIGRAMGEE
EEEEHHHCCEEEEECCCCCCCCCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHHHCCCC
QNIVWGTGTHTDTPVAVIAYGPDQATAPFSSLTHHTEIGKLLIKTVRSGH
CCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLNTVKKTFLALSITLASCSSFAETPVKNVILMIGDGMGPGQMGLLEEYARKAPHSIY
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHC
NGRDTALKTMIDNGVLGMSLHSPADALVVDSACSASQLASGVSAPSEALGIDVDGNPVES
CCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCHHHHHHCCCCCHHHEEECCCCCHHHH
VLEIAKSLGKATGLVSDTRLTHATPAAFASHQPHRSLENQIAEEMLATRVDVMLSGGLRY
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCEEE
WIPMSANEGGAVYQKLLKKTGGDIKIKSKRIDEKNLLTEAEGAGYAVVHTRDALANAPGD
EEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEHHHHCCCCCH
QILGLFAYSGMQDGITYSNTKNSPERSMPSLKEMTIKALDVLSQDPDGFFLMVEGGQIDW
HHHHHHHHCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
AGHNNDAATMLHEMLKFDETVEYVYEWVKNRKDTLVVVTADHETGGFGFSYSRANTPKAE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHH
KRTGIAFKDRDYKPNFNFGNLAILDKLYEQKKSFGNMLAEFDQTDKSPESLLAIVNANSE
HHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
FKISLAQAKEILVTEENEFYDPENKYLKLKTFPKVNDFKAFYVYGEEVRNDLIGRAMGEE
EEEEHHHCCEEEEECCCCCCCCCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHHHCCCC
QNIVWGTGTHTDTPVAVIAYGPDQATAPFSSLTHHTEIGKLLIKTVRSGH
CCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]