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The map label for this gene is ilvE [H]

Identifier: 119946235

GI number: 119946235

Start: 3186510

End: 3187598

Strand: Reverse

Name: ilvE [H]

Synonym: Ping_2596

Alternate gene names: 119946235

Gene position: 3187598-3186510 (Counterclockwise)

Preceding gene: 119946238

Following gene: 119946232

Centisome position: 69.91

GC content: 42.33

Gene sequence:

>1089_bases
ATGATAATTGATATTAAACCGCTTGAGCTTTCCAAGCGGGGGGCTTGTCCAGAAACTTACAGTTTTGGAGCTGATTTTTC
TGACCATATGTTTAGCCAAAATTTTGAACAAGGCAGCGGTTGGGAGAAAGCTGAAATTACCCCTTATCATGCATTAACAC
TTGATCCTTCAGCGGCTGTATTTCATTACGGACAAGAAATATTTGAAGGTTTAAAGGCTTACCGTTCATTCGATGGAACG
ATTAATTTGTTTCGTCCTTCTGAAAATTTCAAACGTTTTAACCGTTCAGCTAAAAGAATGGCGATGCCCGAAATAGATGG
AAAATTTCACCTGAAAGCACTTAAAGCGCTTATCTCGCTTGATCATAATTGGATTCCGGAGCAAGTGGGATCGTCTTTAT
ATATCCGCCCGACAATGATTGCGACGGGTAAAAAGTTGGGTTTAGGGGCGAGTGATTCCTATATTCACTTTATTATCACG
GGGCCAGCCGGATCTTACTTCAAGGGTGGTTTTGAGCCGGTATCGGTTTATATTGCCGATAAGTATCGCCGCGCAGTAGT
GGGGGGAGTGGGTGAAGCTAAGACGGGCGGTAATTATGCCGCCAGCCTGTATGTCTCAGAAGAAGTTGAAAAACTGGGTT
ATTCTCAAGTACTTTGGTTAGATGCTGTACACGGCAAGTATATTGAAGAAGTGGGTGCAATGAATATCTGTTTTGTTTAT
GAAAATAAGCACATTGTAACACCAGCCTTAAGTGGCAGTATATTACCCGGTATTACTCGTGATTCTATTCTTAAACTGGC
GCCGGCATTGGGATTTGAAGTCAGCGAAGAACGCTTAGATATTGAGCAGATATTAGCCGATATAAAAAGTGGTAAAATAA
CGGAAGCATTTGGCTGTGGTACTGCCGCTGTTATCTCTCCGGTTGGTAAACTATGCTGGAAAGGCACTGATTATATTATC
AATAACAATAAATCGGGCGAGATTTCCAAGCAGTTATATGATGAGCTGACCGGTATTCAATACGGGACACGCAAAGACCG
TTTTGGCTGGATAGAGAAGGTTGATCCAGCGGTTACGGTAGACGTTTAA

Upstream 100 bases:

>100_bases
ATTTAATCGTGCTTAACGATAACTTATTATATGTCATAGTTGCTGCTCTTTATGCTCTAGATATTGCATTAAGAAAATAA
TAAAAATACAGGGAATTGAT

Downstream 100 bases:

>100_bases
TTATTCATTTTTACTGTTAAGCACACTAAGAAAAAACCCATTAAGTGATTGATATTTAAATCGCTTAATGGGTTTTTTAA
TATCTGCTACTGGCGTTAGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MIIDIKPLELSKRGACPETYSFGADFSDHMFSQNFEQGSGWEKAEITPYHALTLDPSAAVFHYGQEIFEGLKAYRSFDGT
INLFRPSENFKRFNRSAKRMAMPEIDGKFHLKALKALISLDHNWIPEQVGSSLYIRPTMIATGKKLGLGASDSYIHFIIT
GPAGSYFKGGFEPVSVYIADKYRRAVVGGVGEAKTGGNYAASLYVSEEVEKLGYSQVLWLDAVHGKYIEEVGAMNICFVY
ENKHIVTPALSGSILPGITRDSILKLAPALGFEVSEERLDIEQILADIKSGKITEAFGCGTAAVISPVGKLCWKGTDYII
NNNKSGEISKQLYDELTGIQYGTRKDRFGWIEKVDPAVTVDV

Sequences:

>Translated_362_residues
MIIDIKPLELSKRGACPETYSFGADFSDHMFSQNFEQGSGWEKAEITPYHALTLDPSAAVFHYGQEIFEGLKAYRSFDGT
INLFRPSENFKRFNRSAKRMAMPEIDGKFHLKALKALISLDHNWIPEQVGSSLYIRPTMIATGKKLGLGASDSYIHFIIT
GPAGSYFKGGFEPVSVYIADKYRRAVVGGVGEAKTGGNYAASLYVSEEVEKLGYSQVLWLDAVHGKYIEEVGAMNICFVY
ENKHIVTPALSGSILPGITRDSILKLAPALGFEVSEERLDIEQILADIKSGKITEAFGCGTAAVISPVGKLCWKGTDYII
NNNKSGEISKQLYDELTGIQYGTRKDRFGWIEKVDPAVTVDV
>Mature_362_residues
MIIDIKPLELSKRGACPETYSFGADFSDHMFSQNFEQGSGWEKAEITPYHALTLDPSAAVFHYGQEIFEGLKAYRSFDGT
INLFRPSENFKRFNRSAKRMAMPEIDGKFHLKALKALISLDHNWIPEQVGSSLYIRPTMIATGKKLGLGASDSYIHFIIT
GPAGSYFKGGFEPVSVYIADKYRRAVVGGVGEAKTGGNYAASLYVSEEVEKLGYSQVLWLDAVHGKYIEEVGAMNICFVY
ENKHIVTPALSGSILPGITRDSILKLAPALGFEVSEERLDIEQILADIKSGKITEAFGCGTAAVISPVGKLCWKGTDYII
NNNKSGEISKQLYDELTGIQYGTRKDRFGWIEKVDPAVTVDV

Specific function: Transaminates branched-chain amino acids and ketoglutarate. Involved in the final step of the methionine regeneration pathway, where ketomethiobutyrate (KMTB) is converted to methionine via a transamination. The amino donor preference is isoleucine, leuci

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=39.1304347826087, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=39.1304347826087, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=39.1304347826087, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI50658084, Length=335, Percent_Identity=38.8059701492537, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI258614015, Length=287, Percent_Identity=39.3728222996516, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=33.9130434782609, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI296010900, Length=351, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=31.1526479750779, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=361, Percent_Identity=37.3961218836565, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17565728, Length=348, Percent_Identity=36.4942528735632, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322002, Length=349, Percent_Identity=40.974212034384, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322608, Length=334, Percent_Identity=41.0179640718563, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24641779, Length=344, Percent_Identity=41.2790697674419, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39927; Mature: 39927

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDIKPLELSKRGACPETYSFGADFSDHMFSQNFEQGSGWEKAEITPYHALTLDPSAAV
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHH
FHYGQEIFEGLKAYRSFDGTINLFRPSENFKRFNRSAKRMAMPEIDGKFHLKALKALISL
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DHNWIPEQVGSSLYIRPTMIATGKKLGLGASDSYIHFIITGPAGSYFKGGFEPVSVYIAD
CCCCCHHHHCCEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEH
KYRRAVVGGVGEAKTGGNYAASLYVSEEVEKLGYSQVLWLDAVHGKYIEEVGAMNICFVY
HHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEHHHHHHHHHHCCEEEEEEE
ENKHIVTPALSGSILPGITRDSILKLAPALGFEVSEERLDIEQILADIKSGKITEAFGCG
CCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCEEEHHCCC
TAAVISPVGKLCWKGTDYIINNNKSGEISKQLYDELTGIQYGTRKDRFGWIEKVDPAVTV
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEE
DV
CC
>Mature Secondary Structure
MIIDIKPLELSKRGACPETYSFGADFSDHMFSQNFEQGSGWEKAEITPYHALTLDPSAAV
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHH
FHYGQEIFEGLKAYRSFDGTINLFRPSENFKRFNRSAKRMAMPEIDGKFHLKALKALISL
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DHNWIPEQVGSSLYIRPTMIATGKKLGLGASDSYIHFIITGPAGSYFKGGFEPVSVYIAD
CCCCCHHHHCCEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEH
KYRRAVVGGVGEAKTGGNYAASLYVSEEVEKLGYSQVLWLDAVHGKYIEEVGAMNICFVY
HHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEEHHHHHHHHHHCCEEEEEEE
ENKHIVTPALSGSILPGITRDSILKLAPALGFEVSEERLDIEQILADIKSGKITEAFGCG
CCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCEEEHHCCC
TAAVISPVGKLCWKGTDYIINNNKSGEISKQLYDELTGIQYGTRKDRFGWIEKVDPAVTV
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEE
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]