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The map label for this gene is purR [H]

Identifier: 119946147

GI number: 119946147

Start: 3063471

End: 3064487

Strand: Reverse

Name: purR [H]

Synonym: Ping_2507

Alternate gene names: 119946147

Gene position: 3064487-3063471 (Counterclockwise)

Preceding gene: 119946154

Following gene: 119946146

Centisome position: 67.21

GC content: 43.17

Gene sequence:

>1017_bases
ATGAAAAAAAATAAAAAAATTACAATGAGTGACATCGCCACAGCTGCGGGGGTCTCGCAATCGACAGTTTCACTGGTATT
GAATCGCTCTCAATCAATCAAAATTGCCGAGTCAACCAAGCAGAAGGTGTTGGAAACGGCAGATTCACTTGGTTATAGCA
ATAGGAAAGTGACTCACGCATTGGGCAGAACCCAAAAAATTGCCATGGTGATTAACGGTCTAACCAACTACGATCCCTTT
ATTGACGCCATCAACGCGGCGCGAGAGGAAGCTTGGTCTAATGACTACATTCTGATCACTTTCAACTATGGCAATGATGC
TGAGTTAGCCCATAAGATAGAAGCAGAAGTGAACAGTGGTGAATATGTAGGACTTATTTATGCGTCCAGCATGACACGTG
CACTCAAACAGCAAAAAGTTAACAGCAGCCTGCCAACCGTCTTGCTTAATTGCACTAACGAAGATGCTCCTGAGGCTCAA
TCTATTCTGCCGGCTGATACGATTGGTGCTTATAAAGCAGTTTTTCATCTTACATCTCAAGGGTATCGACGTATTGCGAT
GCTGACAGGTGAAAGCTGGATGTTGGCAACAAGCAAGCGAGTCAGTGGATACCGTCAGGCGTTGATTGATGCGGATATTA
TTCCAAAAGACCATTATATTCTTGAGGCAAACTGGTCTCTAAAAGAAGCTCATCAACAAACCCTTAAGTTACTGGAACTT
GCCACTCCACCAGAAGCTATTTTCTGTGGCAGTGACTATATGGCAATGGGCTGCTACCAGGCGATTGCAGAGAAAGGACT
CAAAATTCCGCAAGATATTGCTGTAGTAGGGTATGATAATCAACCGATTGCTTCTGAGATTTTTCCTGCATTGACCTCGG
TGGAATTACCTTATTCTGTCATGGGTAAGGAAGCTATTCAGTCAATTGTCGCGCGGATTGCGCATCAACCGTTGCTTTGT
ATGCAAAGAAAGATTGAAGGTGAATTGATTGTACGTTCCTCTTCAATAAAAAAATAA

Upstream 100 bases:

>100_bases
GATTTGTTTTTAGAAAGAAATTGTGATCTAACTCCGTAAAGCTTTCAATTGTTAATTACTTTAGCTAATATTATTAACTC
TTATTTAGCTTGGTGATCTT

Downstream 100 bases:

>100_bases
ACTGAGAAGTTCACCAAAAACGTTATCTAAACCATAAACTGATGGATTGTTTGAGACAGTCAAACCTTTTTGATGAACTT
ATAATAACCACCTCCCCGCT

Product: transcriptional regulator, periplasmic binding protein of LacI family protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKKNKKITMSDIATAAGVSQSTVSLVLNRSQSIKIAESTKQKVLETADSLGYSNRKVTHALGRTQKIAMVINGLTNYDPF
IDAINAAREEAWSNDYILITFNYGNDAELAHKIEAEVNSGEYVGLIYASSMTRALKQQKVNSSLPTVLLNCTNEDAPEAQ
SILPADTIGAYKAVFHLTSQGYRRIAMLTGESWMLATSKRVSGYRQALIDADIIPKDHYILEANWSLKEAHQQTLKLLEL
ATPPEAIFCGSDYMAMGCYQAIAEKGLKIPQDIAVVGYDNQPIASEIFPALTSVELPYSVMGKEAIQSIVARIAHQPLLC
MQRKIEGELIVRSSSIKK

Sequences:

>Translated_338_residues
MKKNKKITMSDIATAAGVSQSTVSLVLNRSQSIKIAESTKQKVLETADSLGYSNRKVTHALGRTQKIAMVINGLTNYDPF
IDAINAAREEAWSNDYILITFNYGNDAELAHKIEAEVNSGEYVGLIYASSMTRALKQQKVNSSLPTVLLNCTNEDAPEAQ
SILPADTIGAYKAVFHLTSQGYRRIAMLTGESWMLATSKRVSGYRQALIDADIIPKDHYILEANWSLKEAHQQTLKLLEL
ATPPEAIFCGSDYMAMGCYQAIAEKGLKIPQDIAVVGYDNQPIASEIFPALTSVELPYSVMGKEAIQSIVARIAHQPLLC
MQRKIEGELIVRSSSIKK
>Mature_338_residues
MKKNKKITMSDIATAAGVSQSTVSLVLNRSQSIKIAESTKQKVLETADSLGYSNRKVTHALGRTQKIAMVINGLTNYDPF
IDAINAAREEAWSNDYILITFNYGNDAELAHKIEAEVNSGEYVGLIYASSMTRALKQQKVNSSLPTVLLNCTNEDAPEAQ
SILPADTIGAYKAVFHLTSQGYRRIAMLTGESWMLATSKRVSGYRQALIDADIIPKDHYILEANWSLKEAHQQTLKLLEL
ATPPEAIFCGSDYMAMGCYQAIAEKGLKIPQDIAVVGYDNQPIASEIFPALTSVELPYSVMGKEAIQSIVARIAHQPLLC
MQRKIEGELIVRSSSIKK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=347, Percent_Identity=27.6657060518732, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790194, Length=321, Percent_Identity=29.9065420560748, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=26.3473053892216, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=29.8136645962733, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=27.2435897435897, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=26.4264264264264, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789068, Length=318, Percent_Identity=26.1006289308176, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=26.0586319218241, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787906, Length=331, Percent_Identity=25.9818731117825, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789456, Length=308, Percent_Identity=26.6233766233766, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=24.8447204968944, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=22.0183486238532, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37149; Mature: 37149

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKKITMSDIATAAGVSQSTVSLVLNRSQSIKIAESTKQKVLETADSLGYSNRKVTHA
CCCCCEEEHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHH
LGRTQKIAMVINGLTNYDPFIDAINAAREEAWSNDYILITFNYGNDAELAHKIEAEVNSG
HCCCCEEHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCC
EYVGLIYASSMTRALKQQKVNSSLPTVLLNCTNEDAPEAQSILPADTIGAYKAVFHLTSQ
CEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHC
GYRRIAMLTGESWMLATSKRVSGYRQALIDADIIPKDHYILEANWSLKEAHQQTLKLLEL
CCEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
ATPPEAIFCGSDYMAMGCYQAIAEKGLKIPQDIAVVGYDNQPIASEIFPALTSVELPYSV
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHH
MGKEAIQSIVARIAHQPLLCMQRKIEGELIVRSSSIKK
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MKKNKKITMSDIATAAGVSQSTVSLVLNRSQSIKIAESTKQKVLETADSLGYSNRKVTHA
CCCCCEEEHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHH
LGRTQKIAMVINGLTNYDPFIDAINAAREEAWSNDYILITFNYGNDAELAHKIEAEVNSG
HCCCCEEHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCC
EYVGLIYASSMTRALKQQKVNSSLPTVLLNCTNEDAPEAQSILPADTIGAYKAVFHLTSQ
CEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHC
GYRRIAMLTGESWMLATSKRVSGYRQALIDADIIPKDHYILEANWSLKEAHQQTLKLLEL
CCEEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
ATPPEAIFCGSDYMAMGCYQAIAEKGLKIPQDIAVVGYDNQPIASEIFPALTSVELPYSV
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHH
MGKEAIQSIVARIAHQPLLCMQRKIEGELIVRSSSIKK
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA