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The map label for this gene is cls1 [H]

Identifier: 119946105

GI number: 119946105

Start: 3001514

End: 3002905

Strand: Direct

Name: cls1 [H]

Synonym: Ping_2455

Alternate gene names: 119946105

Gene position: 3001514-3002905 (Clockwise)

Preceding gene: 119946103

Following gene: 119946106

Centisome position: 65.83

GC content: 43.75

Gene sequence:

>1392_bases
ATGCAGAACAACTTTTTTGAGTATTTAACACTCAACAGTGTTTTTATCGTAACCGAAATTATTATCGTGTTTACTATGCT
GCACATGCTCTACCAAAGACGTTCTCCGACGAGTATCATCTCCTGGCTTCTACTGATGATCATTCTCCCCTATTTCTCTC
TTGTACTTTATTTTCTGATAGGTGTTCGCAAACGTCCGGTTAGAAAGAAAAAATCATCTCTGCATGTCAAAAGTACCAGC
GAAAGTAACGGAAAAATTAACAGCATAGACGGTATTATACGCGCTAACGGTCTTGCCGGAGCAAGTGAGCATAACAGTTT
TGAGTTTATCAGTGACAGCGTCAACGCCTACAACACGTTAATAAATGAAATCAAAAATGCGAAAAAGAGCATCAGTTTCA
GTACCTATATCCTAAAACAAGACAAGGTCACTAAAACAATCATTGATGCCCTGATAGAGAAAGAGTCAAGCGGTGTTCAT
GTGCGACTGCTACTCGATTCACTCGGTTCCTATCAACTCTACTTTTTTCAGGGCCCACTGCTAAAACTCAGAAGAGCGGG
ATGCGAAGTCCACTTTTTTATGCCGCTACTTCGTCTGCCCTATCAAAACTCCATTAATCTGCGCAACCATCGTAAAATCT
ATCTCTTTGACGAGACCACCTTGCTTACCGGAGGAATGAATCTATCAAAGAAGTATATGGGGCCCGCAAAAAAACAGGAT
CAGTGCGAAGATATTCTGTTCAAAACTCAAGGGGATGCAGCCTATCAGTATGCCCAGATCTTTGAAGCTGACTGGGCCTA
TGCTTCCGACTCTCCCCAGCGACAAGCGACTCGGCCCTTACAAAAAGTCTGTGGCGATGCTTATATCCAAGTTGTGCCCT
CTGGGCCCGATATCAAAGGTGATGCCCTTTTTGAAGCACTGATCAGTGCAATTCACTCGGCTAAAGAGCGCATCTGGATA
GTCACCCCCTACTTTCTGCCCGATGAGACCTTAATGCATGCGCTAAAGATTGCCAAACACAATGGCGTCGATATCAAACT
TATCACACCTAAGCAGTCCGATCATTTAGTCGCCGACCTTGGCCGCAGTTCCTACATGCGAGAACTGCAGGAAGAAGGAA
TCAATCTGGTCTTATATAATGGTAAGATGCTGCATGCCAAAGCCATTCTGTTTGACCAGGATGCCGTTATGATAGGCTCA
GTCAATATTGACAACCGCTCCCTGCTGCTTAATTACGAAGTTGTCAGCTTTGCCTATTCTAAAGCTATTATCTCCGAGAT
AGAAAAATGGATGCAGGGTTTTATCACCAATGCTGACACGCAAATGAAAGAAGCCTCCAGGCTCCGTTGTATTGCAGAAA
ATTTTATGCGTATCTTAGCCCCTCAACTCTAG

Upstream 100 bases:

>100_bases
TCTTTAGTGGCCCGCTTATTCTCTGGCTCTATAATCTAAAGCGCAATCTCACCTTGAAGGCGATTTTTCTCTGATTATCC
AAGACATATGGAACTTTTTT

Downstream 100 bases:

>100_bases
GGTCTGCTGACCTTTGGAGTACAAATTTTGTTCAATTTAAACGTATTCTGAGCAAGGCATGCTTCGTTCACATAGTTATT
CTATGTAAACGACGCATAAC

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MQNNFFEYLTLNSVFIVTEIIIVFTMLHMLYQRRSPTSIISWLLLMIILPYFSLVLYFLIGVRKRPVRKKKSSLHVKSTS
ESNGKINSIDGIIRANGLAGASEHNSFEFISDSVNAYNTLINEIKNAKKSISFSTYILKQDKVTKTIIDALIEKESSGVH
VRLLLDSLGSYQLYFFQGPLLKLRRAGCEVHFFMPLLRLPYQNSINLRNHRKIYLFDETTLLTGGMNLSKKYMGPAKKQD
QCEDILFKTQGDAAYQYAQIFEADWAYASDSPQRQATRPLQKVCGDAYIQVVPSGPDIKGDALFEALISAIHSAKERIWI
VTPYFLPDETLMHALKIAKHNGVDIKLITPKQSDHLVADLGRSSYMRELQEEGINLVLYNGKMLHAKAILFDQDAVMIGS
VNIDNRSLLLNYEVVSFAYSKAIISEIEKWMQGFITNADTQMKEASRLRCIAENFMRILAPQL

Sequences:

>Translated_463_residues
MQNNFFEYLTLNSVFIVTEIIIVFTMLHMLYQRRSPTSIISWLLLMIILPYFSLVLYFLIGVRKRPVRKKKSSLHVKSTS
ESNGKINSIDGIIRANGLAGASEHNSFEFISDSVNAYNTLINEIKNAKKSISFSTYILKQDKVTKTIIDALIEKESSGVH
VRLLLDSLGSYQLYFFQGPLLKLRRAGCEVHFFMPLLRLPYQNSINLRNHRKIYLFDETTLLTGGMNLSKKYMGPAKKQD
QCEDILFKTQGDAAYQYAQIFEADWAYASDSPQRQATRPLQKVCGDAYIQVVPSGPDIKGDALFEALISAIHSAKERIWI
VTPYFLPDETLMHALKIAKHNGVDIKLITPKQSDHLVADLGRSSYMRELQEEGINLVLYNGKMLHAKAILFDQDAVMIGS
VNIDNRSLLLNYEVVSFAYSKAIISEIEKWMQGFITNADTQMKEASRLRCIAENFMRILAPQL
>Mature_463_residues
MQNNFFEYLTLNSVFIVTEIIIVFTMLHMLYQRRSPTSIISWLLLMIILPYFSLVLYFLIGVRKRPVRKKKSSLHVKSTS
ESNGKINSIDGIIRANGLAGASEHNSFEFISDSVNAYNTLINEIKNAKKSISFSTYILKQDKVTKTIIDALIEKESSGVH
VRLLLDSLGSYQLYFFQGPLLKLRRAGCEVHFFMPLLRLPYQNSINLRNHRKIYLFDETTLLTGGMNLSKKYMGPAKKQD
QCEDILFKTQGDAAYQYAQIFEADWAYASDSPQRQATRPLQKVCGDAYIQVVPSGPDIKGDALFEALISAIHSAKERIWI
VTPYFLPDETLMHALKIAKHNGVDIKLITPKQSDHLVADLGRSSYMRELQEEGINLVLYNGKMLHAKAILFDQDAVMIGS
VNIDNRSLLLNYEVVSFAYSKAIISEIEKWMQGFITNADTQMKEASRLRCIAENFMRILAPQL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=421, Percent_Identity=28.2660332541568, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787007, Length=361, Percent_Identity=26.5927977839335, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52694; Mature: 52694

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNNFFEYLTLNSVFIVTEIIIVFTMLHMLYQRRSPTSIISWLLLMIILPYFSLVLYFLI
CCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVRKRPVRKKKSSLHVKSTSESNGKINSIDGIIRANGLAGASEHNSFEFISDSVNAYNTL
CCCCCCCHHHHCCEEEEECCCCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
INEIKNAKKSISFSTYILKQDKVTKTIIDALIEKESSGVHVRLLLDSLGSYQLYFFQGPL
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCHH
LKLRRAGCEVHFFMPLLRLPYQNSINLRNHRKIYLFDETTLLTGGMNLSKKYMGPAKKQD
HHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCEEEEEECCEEEECCCCCCHHHCCCCCCCH
QCEDILFKTQGDAAYQYAQIFEADWAYASDSPQRQATRPLQKVCGDAYIQVVPSGPDIKG
HHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCH
DALFEALISAIHSAKERIWIVTPYFLPDETLMHALKIAKHNGVDIKLITPKQSDHLVADL
HHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
GRSSYMRELQEEGINLVLYNGKMLHAKAILFDQDAVMIGSVNIDNRSLLLNYEVVSFAYS
CHHHHHHHHHHCCCEEEEECCEEEEEEEEEEECCEEEEEEEEECCCEEEEEHHHHHHHHH
KAIISEIEKWMQGFITNADTQMKEASRLRCIAENFMRILAPQL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQNNFFEYLTLNSVFIVTEIIIVFTMLHMLYQRRSPTSIISWLLLMIILPYFSLVLYFLI
CCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVRKRPVRKKKSSLHVKSTSESNGKINSIDGIIRANGLAGASEHNSFEFISDSVNAYNTL
CCCCCCCHHHHCCEEEEECCCCCCCEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
INEIKNAKKSISFSTYILKQDKVTKTIIDALIEKESSGVHVRLLLDSLGSYQLYFFQGPL
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCHH
LKLRRAGCEVHFFMPLLRLPYQNSINLRNHRKIYLFDETTLLTGGMNLSKKYMGPAKKQD
HHHHHCCCCEEEHHHHHHCCCCCCCCCCCCCEEEEEECCEEEECCCCCCHHHCCCCCCCH
QCEDILFKTQGDAAYQYAQIFEADWAYASDSPQRQATRPLQKVCGDAYIQVVPSGPDIKG
HHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCH
DALFEALISAIHSAKERIWIVTPYFLPDETLMHALKIAKHNGVDIKLITPKQSDHLVADL
HHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
GRSSYMRELQEEGINLVLYNGKMLHAKAILFDQDAVMIGSVNIDNRSLLLNYEVVSFAYS
CHHHHHHHHHHCCCEEEEECCEEEEEEEEEEECCEEEEEEEEECCCEEEEEHHHHHHHHH
KAIISEIEKWMQGFITNADTQMKEASRLRCIAENFMRILAPQL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]