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The map label for this gene is gatA [H]

Identifier: 119946068

GI number: 119946068

Start: 2962284

End: 2964086

Strand: Direct

Name: gatA [H]

Synonym: Ping_2417

Alternate gene names: 119946068

Gene position: 2962284-2964086 (Clockwise)

Preceding gene: 119946063

Following gene: 119946069

Centisome position: 64.97

GC content: 45.09

Gene sequence:

>1803_bases
ATGTTAACTAACCTTCCCTTTACCATAGATGCTTTGCACCAGGCCTATCGTCAAGGGGTCAGTGCTGATCAAGTGATCCG
TGAATCACTAACGCGTCTTTCAAATATCGCAGATGAAGGTATTTATCTGTATGTTGCCAGTGATTCACAGTTGTCGAAGG
CTGTTGCAGATCTTGGTCCCTTCGATCTTACGCTAAAGCCGTTATGGGGAATTCCCTTTGCCGTCAAAGATAATATTGAT
GCAGTAGATATGCCTACGACTGCGGCCTGCAAGGCGTTTAGTTATATTGCCAGGCAAGATGCTTATGTGGTTTCTTTATT
GAAACAGGCAGGGGCAATCCTTATTGGTAAAACCAATTTAGATCAATTTGCAACCGGTTTAGTGGGTTTAAGAACACCTT
ATCCCGCACCTAAAAATGCAATTGATGCAAAACTAGTACCCGGTGGTTCTAGTTCGGGGTCAGCGGTGGCAGTGGCACAT
GGGCAAGTCTGCTTTTCACTTGGAACTGATACTGCAGGTTCAGGGCGCATACCTGCAGCCCTCAATAATATTGTCGGCCT
CAAACCGACGCTGGGCAGTATCTCCAGCCGAGGAGTGATCCCCGCCTGTCGTTCACTCGACACAGTATCGATATTGGCGC
TTACTGTTGAAAATGCTGCACAAGTGTATGCTTGCTGTCGATACTACGATCAACAAGATCCCTTTTCTAAACCCTATCAC
GGCCCTGCTAAGCAGAATATACCCGTTAATTTTACTGTTGCAGTCCCTGATTCAGAAAGCCTGATATTAGATGAACCCGC
TCAAAAGAAAGCTTATGAGCAAGCCCTTAACTACTGGCGACAAAAAGGCGCGAAGCTGGTTGAAATTGACTTTAAACCTT
TTTATGAAGTCTCTAAATTATTATATGAAGGCCCCTGGGTTGCGGAAAGACTCGCGGCATTAGCGCCTTTTATGGCAAAG
CATCGCAGCGCGCTGTATCCGGTAACTTGTGAGATTATTGAATCTGCGCATAAATTCTCCGCAAGTGATGCATTTAATGC
CTACTATCGCCTGCGGGCACTTTCCAGGCAAATAGCGTCAATACTAACCAAGATCGACGTCATCTGTGTTCCGTCAATGC
CGGGGCTAGTTTACAAAAGTGACATAGAGGTCGACCCTATAGGCCCAAATAGTCGTCTCGGCACTTACACTAACTTTGTT
AATTTACTTGATTTATGTGCTATCGCGATACCGGGAAAAACGCGTAGCGATGGTTATCCATCAAGCATTACGCTTATTGC
ACAAAATGGTTGTGATGCCTTTATTCATGCGCTGGCAGCTGATTATCATGCAAATCAAGGTGATTATTTAGGTGCAACAA
GTCATAAAATCCCCGTTCCTGATCGCCTCACAACGACCACTAATATAATGCAAATGACAAGTAACCATGAAATAGCGATT
GCGGTCGTAGGAGCACATATGACGAACCTCCCGCTTAATCACCAACTAACAGATAAAGGAGGTCGTTTTCTGACCTCTGC
CCAGACAGATTCACACTATCGTTTATACCAACTTGGTAATCGTCCAGGGCTTATTAGAGATGATGATGGGCAGATAATTG
ACCTTGAAGTCTGGGCCTTACCCAAAAACAAGATCGGTGATTTGATTGCCCAAATACCCTCACCCCTCAGCATTGGTTCG
ATTACGCTGTCAGATAAGCAACAAGTAAAAGGTTTTTTATGTGAGCCTCATGGGCTCAAAGATAGCCGTGACATTACTCA
TTTCGGCGGGTGGCGAGCATTTTGCCAAGCTGAAAGTCTTTAG

Upstream 100 bases:

>100_bases
TGCGATGCAAAATATGCACCACACAAATGCAAAACAAGCAGTATACAGCATCACTATTAATTGGTTAACTACTGATATCG
TTTAATACAGGACAGTCATA

Downstream 100 bases:

>100_bases
TTACACATTTTCTACATTTTTCTATCTATGGAGCGATTATGCTAAATAATCTAAAAAGTAAAATAAAATTACCAACCAAA
GCGGGCTTGTATGCTTTGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MLTNLPFTIDALHQAYRQGVSADQVIRESLTRLSNIADEGIYLYVASDSQLSKAVADLGPFDLTLKPLWGIPFAVKDNID
AVDMPTTAACKAFSYIARQDAYVVSLLKQAGAILIGKTNLDQFATGLVGLRTPYPAPKNAIDAKLVPGGSSSGSAVAVAH
GQVCFSLGTDTAGSGRIPAALNNIVGLKPTLGSISSRGVIPACRSLDTVSILALTVENAAQVYACCRYYDQQDPFSKPYH
GPAKQNIPVNFTVAVPDSESLILDEPAQKKAYEQALNYWRQKGAKLVEIDFKPFYEVSKLLYEGPWVAERLAALAPFMAK
HRSALYPVTCEIIESAHKFSASDAFNAYYRLRALSRQIASILTKIDVICVPSMPGLVYKSDIEVDPIGPNSRLGTYTNFV
NLLDLCAIAIPGKTRSDGYPSSITLIAQNGCDAFIHALAADYHANQGDYLGATSHKIPVPDRLTTTTNIMQMTSNHEIAI
AVVGAHMTNLPLNHQLTDKGGRFLTSAQTDSHYRLYQLGNRPGLIRDDDGQIIDLEVWALPKNKIGDLIAQIPSPLSIGS
ITLSDKQQVKGFLCEPHGLKDSRDITHFGGWRAFCQAESL

Sequences:

>Translated_600_residues
MLTNLPFTIDALHQAYRQGVSADQVIRESLTRLSNIADEGIYLYVASDSQLSKAVADLGPFDLTLKPLWGIPFAVKDNID
AVDMPTTAACKAFSYIARQDAYVVSLLKQAGAILIGKTNLDQFATGLVGLRTPYPAPKNAIDAKLVPGGSSSGSAVAVAH
GQVCFSLGTDTAGSGRIPAALNNIVGLKPTLGSISSRGVIPACRSLDTVSILALTVENAAQVYACCRYYDQQDPFSKPYH
GPAKQNIPVNFTVAVPDSESLILDEPAQKKAYEQALNYWRQKGAKLVEIDFKPFYEVSKLLYEGPWVAERLAALAPFMAK
HRSALYPVTCEIIESAHKFSASDAFNAYYRLRALSRQIASILTKIDVICVPSMPGLVYKSDIEVDPIGPNSRLGTYTNFV
NLLDLCAIAIPGKTRSDGYPSSITLIAQNGCDAFIHALAADYHANQGDYLGATSHKIPVPDRLTTTTNIMQMTSNHEIAI
AVVGAHMTNLPLNHQLTDKGGRFLTSAQTDSHYRLYQLGNRPGLIRDDDGQIIDLEVWALPKNKIGDLIAQIPSPLSIGS
ITLSDKQQVKGFLCEPHGLKDSRDITHFGGWRAFCQAESL
>Mature_600_residues
MLTNLPFTIDALHQAYRQGVSADQVIRESLTRLSNIADEGIYLYVASDSQLSKAVADLGPFDLTLKPLWGIPFAVKDNID
AVDMPTTAACKAFSYIARQDAYVVSLLKQAGAILIGKTNLDQFATGLVGLRTPYPAPKNAIDAKLVPGGSSSGSAVAVAH
GQVCFSLGTDTAGSGRIPAALNNIVGLKPTLGSISSRGVIPACRSLDTVSILALTVENAAQVYACCRYYDQQDPFSKPYH
GPAKQNIPVNFTVAVPDSESLILDEPAQKKAYEQALNYWRQKGAKLVEIDFKPFYEVSKLLYEGPWVAERLAALAPFMAK
HRSALYPVTCEIIESAHKFSASDAFNAYYRLRALSRQIASILTKIDVICVPSMPGLVYKSDIEVDPIGPNSRLGTYTNFV
NLLDLCAIAIPGKTRSDGYPSSITLIAQNGCDAFIHALAADYHANQGDYLGATSHKIPVPDRLTTTTNIMQMTSNHEIAI
AVVGAHMTNLPLNHQLTDKGGRFLTSAQTDSHYRLYQLGNRPGLIRDDDGQIIDLEVWALPKNKIGDLIAQIPSPLSIGS
ITLSDKQQVKGFLCEPHGLKDSRDITHFGGWRAFCQAESL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=480, Percent_Identity=26.25, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI195972892, Length=220, Percent_Identity=30, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=391, Percent_Identity=27.1099744245524, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=127, Percent_Identity=39.3700787401575, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=157, Percent_Identity=34.3949044585987, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=551, Percent_Identity=42.2867513611615, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323950, Length=177, Percent_Identity=43.5028248587571, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=261, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=160, Percent_Identity=38.75, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652985, Length=423, Percent_Identity=24.5862884160757, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922090, Length=423, Percent_Identity=24.5862884160757, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652981, Length=423, Percent_Identity=24.5862884160757, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652983, Length=423, Percent_Identity=24.5862884160757, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45550774, Length=140, Percent_Identity=37.1428571428571, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648435, Length=140, Percent_Identity=37.1428571428571, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648437, Length=140, Percent_Identity=37.1428571428571, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648441, Length=140, Percent_Identity=37.1428571428571, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=140, Percent_Identity=37.1428571428571, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=138, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 65001; Mature: 65001

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNLPFTIDALHQAYRQGVSADQVIRESLTRLSNIADEGIYLYVASDSQLSKAVADLGP
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCC
FDLTLKPLWGIPFAVKDNIDAVDMPTTAACKAFSYIARQDAYVVSLLKQAGAILIGKTNL
CCEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
DQFATGLVGLRTPYPAPKNAIDAKLVPGGSSSGSAVAVAHGQVCFSLGTDTAGSGRIPAA
HHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
LNNIVGLKPTLGSISSRGVIPACRSLDTVSILALTVENAAQVYACCRYYDQQDPFSKPYH
HHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GPAKQNIPVNFTVAVPDSESLILDEPAQKKAYEQALNYWRQKGAKLVEIDFKPFYEVSKL
CCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LYEGPWVAERLAALAPFMAKHRSALYPVTCEIIESAHKFSASDAFNAYYRLRALSRQIAS
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ILTKIDVICVPSMPGLVYKSDIEVDPIGPNSRLGTYTNFVNLLDLCAIAIPGKTRSDGYP
HHHHHCEEEECCCCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SSITLIAQNGCDAFIHALAADYHANQGDYLGATSHKIPVPDRLTTTTNIMQMTSNHEIAI
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEE
AVVGAHMTNLPLNHQLTDKGGRFLTSAQTDSHYRLYQLGNRPGLIRDDDGQIIDLEVWAL
EEEECHHHCCCCCCEECCCCCEEEEECCCCCCEEEEEECCCCCCEECCCCCEEEEEEEEC
PKNKIGDLIAQIPSPLSIGSITLSDKQQVKGFLCEPHGLKDSRDITHFGGWRAFCQAESL
CCHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEECCCCCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MLTNLPFTIDALHQAYRQGVSADQVIRESLTRLSNIADEGIYLYVASDSQLSKAVADLGP
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCC
FDLTLKPLWGIPFAVKDNIDAVDMPTTAACKAFSYIARQDAYVVSLLKQAGAILIGKTNL
CCEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
DQFATGLVGLRTPYPAPKNAIDAKLVPGGSSSGSAVAVAHGQVCFSLGTDTAGSGRIPAA
HHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
LNNIVGLKPTLGSISSRGVIPACRSLDTVSILALTVENAAQVYACCRYYDQQDPFSKPYH
HHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GPAKQNIPVNFTVAVPDSESLILDEPAQKKAYEQALNYWRQKGAKLVEIDFKPFYEVSKL
CCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
LYEGPWVAERLAALAPFMAKHRSALYPVTCEIIESAHKFSASDAFNAYYRLRALSRQIAS
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ILTKIDVICVPSMPGLVYKSDIEVDPIGPNSRLGTYTNFVNLLDLCAIAIPGKTRSDGYP
HHHHHCEEEECCCCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SSITLIAQNGCDAFIHALAADYHANQGDYLGATSHKIPVPDRLTTTTNIMQMTSNHEIAI
CEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCEEE
AVVGAHMTNLPLNHQLTDKGGRFLTSAQTDSHYRLYQLGNRPGLIRDDDGQIIDLEVWAL
EEEECHHHCCCCCCEECCCCCEEEEECCCCCCEEEEEECCCCCCEECCCCCEEEEEEEEC
PKNKIGDLIAQIPSPLSIGSITLSDKQQVKGFLCEPHGLKDSRDITHFGGWRAFCQAESL
CCHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEECCCCCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA