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The map label for this gene is mptN [H]

Identifier: 119945982

GI number: 119945982

Start: 2823083

End: 2823973

Strand: Reverse

Name: mptN [H]

Synonym: Ping_2322

Alternate gene names: 119945982

Gene position: 2823973-2823083 (Counterclockwise)

Preceding gene: 119945983

Following gene: 119945981

Centisome position: 61.93

GC content: 45.34

Gene sequence:

>891_bases
TTGAATAATTTAAAAGTTGGCGTTATTGGTATTCCGGGTAAATGGTCGACAGAAGTGCTGGCTGACCGCCTTGAAGAGAT
GACTGGTTTTCGCTTAGTAATCGATATGGCAGAAGTGACCCTTGATCTTGCGTCTAAGCATTTAATGTTTCGTGGTATTA
ATCTTTGCGAGCTCGATGGATTGATTGTAAAAAAAATCAGCCAAGCCTATTCACCCTCGATGCTGGATCGCATTGAGTTG
CTGCGTGTTGCGGAGCAGGCAGGGGTAAGGGTTTTTTCTCGCCCCGAAAGTATTTTACGGCTAGTTGATCGTTTGAGCTG
TACCGTGACGTTACGTAATCATGATATCCCAATGCCAAAAACCTGTGTGACCGAATCGCTAGATGCGGCTGTGTCGGCCA
TTGAGGAATATGGTGAAGTGGTATTAAAACCCTTATTCTCAACGAAAGCGCGCGGCATGACATTACTAAATGCCAAGCAG
CCTCGCGATGAATTACGCTCACAACTGAGTCTATTTCAGCAAGATAACCCCATGCTGTACTTGCAGCAAAAATGTGATCT
TCATGGGCAGGATCTTGGTATTGTATTTTTGGGCGGGGAGTATATTGGAACCTATGCGCGGGTATCACAAAGTGATAGCT
GGAATACCACGATATTAGCAGGTGGTGAATATAAGCCCTTTACACCCGATGATGAGGTGATAGCGCTCGCCAAAAAAGCC
CAGCAGCCCTTTGCCCTCGATTTCACAACCGTTGATGTCGCTTTTACGGATGATGGGCCAATCGTATTTGAAGTGTCTGC
TTTTGGTGGTTTTAAAGGCGCGCTGGACGGATGCGGTATTGATGCGGCAAAATTGTATAGCCAGTATATTATTGATTCTT
TTGCTGGTTAA

Upstream 100 bases:

>100_bases
GAGCGTATGTATTTTCGCGCGCGCTATTTACTAGAGCGCTGTTTAGCTGTTGAACGGGGAAAAGAATCTAGGTTCAAATT
ACCTTTTGGAGACTTATTTT

Downstream 100 bases:

>100_bases
ATGCGACTCTTCGCAAATGCACTTTTACTTTATTAACTTATTTTTGCAGTGGTTTTGATACGAATATTATTTGATTGAAC
AGTATTAAACGGCGGTGAAT

Product: RimK domain-containing protein ATP-grasp

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNNLKVGVIGIPGKWSTEVLADRLEEMTGFRLVIDMAEVTLDLASKHLMFRGINLCELDGLIVKKISQAYSPSMLDRIEL
LRVAEQAGVRVFSRPESILRLVDRLSCTVTLRNHDIPMPKTCVTESLDAAVSAIEEYGEVVLKPLFSTKARGMTLLNAKQ
PRDELRSQLSLFQQDNPMLYLQQKCDLHGQDLGIVFLGGEYIGTYARVSQSDSWNTTILAGGEYKPFTPDDEVIALAKKA
QQPFALDFTTVDVAFTDDGPIVFEVSAFGGFKGALDGCGIDAAKLYSQYIIDSFAG

Sequences:

>Translated_296_residues
MNNLKVGVIGIPGKWSTEVLADRLEEMTGFRLVIDMAEVTLDLASKHLMFRGINLCELDGLIVKKISQAYSPSMLDRIEL
LRVAEQAGVRVFSRPESILRLVDRLSCTVTLRNHDIPMPKTCVTESLDAAVSAIEEYGEVVLKPLFSTKARGMTLLNAKQ
PRDELRSQLSLFQQDNPMLYLQQKCDLHGQDLGIVFLGGEYIGTYARVSQSDSWNTTILAGGEYKPFTPDDEVIALAKKA
QQPFALDFTTVDVAFTDDGPIVFEVSAFGGFKGALDGCGIDAAKLYSQYIIDSFAG
>Mature_296_residues
MNNLKVGVIGIPGKWSTEVLADRLEEMTGFRLVIDMAEVTLDLASKHLMFRGINLCELDGLIVKKISQAYSPSMLDRIEL
LRVAEQAGVRVFSRPESILRLVDRLSCTVTLRNHDIPMPKTCVTESLDAAVSAIEEYGEVVLKPLFSTKARGMTLLNAKQ
PRDELRSQLSLFQQDNPMLYLQQKCDLHGQDLGIVFLGGEYIGTYARVSQSDSWNTTILAGGEYKPFTPDDEVIALAKKA
QQPFALDFTTVDVAFTDDGPIVFEVSAFGGFKGALDGCGIDAAKLYSQYIIDSFAG

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI134254462, Length=270, Percent_Identity=26.2962962962963, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787076, Length=260, Percent_Identity=24.6153846153846, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32597; Mature: 32597

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKVGVIGIPGKWSTEVLADRLEEMTGFRLVIDMAEVTLDLASKHLMFRGINLCELDG
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEHHCH
LIVKKISQAYSPSMLDRIELLRVAEQAGVRVFSRPESILRLVDRLSCTVTLRNHDIPMPK
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHEEEEEEECCCCCCCH
TCVTESLDAAVSAIEEYGEVVLKPLFSTKARGMTLLNAKQPRDELRSQLSLFQQDNPMLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEE
LQQKCDLHGQDLGIVFLGGEYIGTYARVSQSDSWNTTILAGGEYKPFTPDDEVIALAKKA
EEECCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHC
QQPFALDFTTVDVAFTDDGPIVFEVSAFGGFKGALDGCGIDAAKLYSQYIIDSFAG
CCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNLKVGVIGIPGKWSTEVLADRLEEMTGFRLVIDMAEVTLDLASKHLMFRGINLCELDG
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEHHCH
LIVKKISQAYSPSMLDRIELLRVAEQAGVRVFSRPESILRLVDRLSCTVTLRNHDIPMPK
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHEEEEEEECCCCCCCH
TCVTESLDAAVSAIEEYGEVVLKPLFSTKARGMTLLNAKQPRDELRSQLSLFQQDNPMLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEE
LQQKCDLHGQDLGIVFLGGEYIGTYARVSQSDSWNTTILAGGEYKPFTPDDEVIALAKKA
EEECCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHC
QQPFALDFTTVDVAFTDDGPIVFEVSAFGGFKGALDGCGIDAAKLYSQYIIDSFAG
CCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]