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The map label for this gene is sucB [H]

Identifier: 119945911

GI number: 119945911

Start: 2735542

End: 2736741

Strand: Reverse

Name: sucB [H]

Synonym: Ping_2251

Alternate gene names: 119945911

Gene position: 2736741-2735542 (Counterclockwise)

Preceding gene: 119945912

Following gene: 119945910

Centisome position: 60.02

GC content: 41.08

Gene sequence:

>1200_bases
ATGATTGAAATATTGGTTCCCGAGCTTCCAGAATCAGTTGCTGATGCAAGTGTTGCCATTTGGCATAAGCAACCTGGTGA
TTTTGTTGAGCGTGATGAAGTACTTGTTGAAATAGAAACCGACAAAGTAGTGTTAGAAGTGCCAGCAACTGCCTCTGGTG
TATTGGAAAGCATCATCGAAAATGAAGGGGCAACCGTCCTTTCAAAGCAATTGTTGGGTCAATTAAAAGAAGGTGCAGCA
GCGCCGGTAAAAGAGCAAAAAACTGAAACTGATGAAGCGCCTGCTGAATCTGCTACTGACACCGCTGCTACTGCTACTGA
CACTGCAGATGCAAGCCCATCGGTGCGACGTTTAATTCTTGAAAAAGGGTTAAATGCCGCCGATATCAAGGGAACAGGCA
AAGGCGGTTCTATTACACGTGAAGATGTAGAAAAACATCGAGTCACAAAAAATCAGGAACAAACTGAACCAAAAGTTGAC
ATTGTTGCTGCGGTAGCTGCTCGCAGCGATAAGCGCGTACCAATGACACGTTTGCGTAAGCGTGTTGCTGAGCGTTTATT
AGAAGCAAAAAATTCTACTGCAATGTTAACCACCTTTAATGAAGTTAACATGAAACCGATTATGGATCTTCGTCGCCAAT
ATCAAGAAGTTTTTGAGAAGAAACACGGTGTACGTTTAGGATTTATGTCGTTTTATATTAAAGCTGTGACAGAAGCACTG
AAACGATTCCCTGAAGTGAATGCGGCTATTGATGGTGATGAAATTGTTTATCATAATTTCTTTGATATCAGTATTGCTGT
TTCAACACCTCGCGGTTTAGTGACACCTGTTTTACGTGATGTGGATGATTTAAATTTTGCTGAAATCGAAAAGGGTATAA
AAGTACTTGCGATTAAAGGCCGTGATGGAAAATTAACCGTTGATGAAATGATTGGTGGTAACTTCACTATTACAAACGGC
GGGGTATTTGGTTCTTTATTATCAACGCCTATTATTAACCCTCCACAAGCCGCCATCCTAGGCATGCATAAAATTCAAGA
TCGTCCTGTGGCAGTTGACGGTAAAGTGGAAATTTTACCGATGATGTATCTGGCACTTTCTTATGATCACCGTTTAATCG
ACGGGAAAGAATCAGTTAGCTTCTTAGTCGCAATCAAAGAATTGCTTGAAGATCCAACGCGCTTATTATTGGATGTTTAA

Upstream 100 bases:

>100_bases
CAAAAAGCATTAATTAATGACGCACTGGCCGTTGTTGAATAATATTTAAAACAAACCCGAATTTTTTAGTTTAGGGTTTT
TACCCTAGGAAAGGTCAATT

Downstream 100 bases:

>100_bases
GGGGTAGTCCAGCGCTGTTTATATCTTCCGTAATTTAGTCAATATAACCGGCGCTAACGTAAGTAACATTACTTCGAGTT
ATGACCTGATTAATAGGGTC

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MIEILVPELPESVADASVAIWHKQPGDFVERDEVLVEIETDKVVLEVPATASGVLESIIENEGATVLSKQLLGQLKEGAA
APVKEQKTETDEAPAESATDTAATATDTADASPSVRRLILEKGLNAADIKGTGKGGSITREDVEKHRVTKNQEQTEPKVD
IVAAVAARSDKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRRQYQEVFEKKHGVRLGFMSFYIKAVTEAL
KRFPEVNAAIDGDEIVYHNFFDISIAVSTPRGLVTPVLRDVDDLNFAEIEKGIKVLAIKGRDGKLTVDEMIGGNFTITNG
GVFGSLLSTPIINPPQAAILGMHKIQDRPVAVDGKVEILPMMYLALSYDHRLIDGKESVSFLVAIKELLEDPTRLLLDV

Sequences:

>Translated_399_residues
MIEILVPELPESVADASVAIWHKQPGDFVERDEVLVEIETDKVVLEVPATASGVLESIIENEGATVLSKQLLGQLKEGAA
APVKEQKTETDEAPAESATDTAATATDTADASPSVRRLILEKGLNAADIKGTGKGGSITREDVEKHRVTKNQEQTEPKVD
IVAAVAARSDKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRRQYQEVFEKKHGVRLGFMSFYIKAVTEAL
KRFPEVNAAIDGDEIVYHNFFDISIAVSTPRGLVTPVLRDVDDLNFAEIEKGIKVLAIKGRDGKLTVDEMIGGNFTITNG
GVFGSLLSTPIINPPQAAILGMHKIQDRPVAVDGKVEILPMMYLALSYDHRLIDGKESVSFLVAIKELLEDPTRLLLDV
>Mature_399_residues
MIEILVPELPESVADASVAIWHKQPGDFVERDEVLVEIETDKVVLEVPATASGVLESIIENEGATVLSKQLLGQLKEGAA
APVKEQKTETDEAPAESATDTAATATDTADASPSVRRLILEKGLNAADIKGTGKGGSITREDVEKHRVTKNQEQTEPKVD
IVAAVAARSDKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRRQYQEVFEKKHGVRLGFMSFYIKAVTEAL
KRFPEVNAAIDGDEIVYHNFFDISIAVSTPRGLVTPVLRDVDDLNFAEIEKGIKVLAIKGRDGKLTVDEMIGGNFTITNG
GVFGSLLSTPIINPPQAAILGMHKIQDRPVAVDGKVEILPMMYLALSYDHRLIDGKESVSFLVAIKELLEDPTRLLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=57.7405857740586, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=28.3062645011601, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=27.4944567627494, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=27.4944567627494, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=25.4716981132075, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=70.3980099502487, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=31.6953316953317, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=412, Percent_Identity=43.6893203883495, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=28.9293849658314, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=418, Percent_Identity=25.3588516746411, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=31.6923076923077, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=43.2098765432099, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=26.9487750556793, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24645909, Length=228, Percent_Identity=57.0175438596491, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18859875, Length=419, Percent_Identity=28.4009546539379, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=32.6460481099656, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=32.6460481099656, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43674; Mature: 43674

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEILVPELPESVADASVAIWHKQPGDFVERDEVLVEIETDKVVLEVPATASGVLESIIE
CCEEECCCCCHHHHCCEEEEEECCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHHH
NEGATVLSKQLLGQLKEGAAAPVKEQKTETDEAPAESATDTAATATDTADASPSVRRLIL
CCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EKGLNAADIKGTGKGGSITREDVEKHRVTKNQEQTEPKVDIVAAVAARSDKRVPMTRLRK
HCCCCEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHH
RVAERLLEAKNSTAMLTTFNEVNMKPIMDLRRQYQEVFEKKHGVRLGFMSFYIKAVTEAL
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
KRFPEVNAAIDGDEIVYHNFFDISIAVSTPRGLVTPVLRDVDDLNFAEIEKGIKVLAIKG
HHCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHCCCEEEEEEC
RDGKLTVDEMIGGNFTITNGGVFGSLLSTPIINPPQAAILGMHKIQDRPVAVDGKVEILP
CCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHH
MMYLALSYDHRLIDGKESVSFLVAIKELLEDPTRLLLDV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MIEILVPELPESVADASVAIWHKQPGDFVERDEVLVEIETDKVVLEVPATASGVLESIIE
CCEEECCCCCHHHHCCEEEEEECCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHHH
NEGATVLSKQLLGQLKEGAAAPVKEQKTETDEAPAESATDTAATATDTADASPSVRRLIL
CCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EKGLNAADIKGTGKGGSITREDVEKHRVTKNQEQTEPKVDIVAAVAARSDKRVPMTRLRK
HCCCCEECCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHH
RVAERLLEAKNSTAMLTTFNEVNMKPIMDLRRQYQEVFEKKHGVRLGFMSFYIKAVTEAL
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
KRFPEVNAAIDGDEIVYHNFFDISIAVSTPRGLVTPVLRDVDDLNFAEIEKGIKVLAIKG
HHCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHCCCEEEEEEC
RDGKLTVDEMIGGNFTITNGGVFGSLLSTPIINPPQAAILGMHKIQDRPVAVDGKVEILP
CCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHH
MMYLALSYDHRLIDGKESVSFLVAIKELLEDPTRLLLDV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]