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The map label for this gene is leuB [H]

Identifier: 119945889

GI number: 119945889

Start: 2711987

End: 2713093

Strand: Reverse

Name: leuB [H]

Synonym: Ping_2229

Alternate gene names: 119945889

Gene position: 2713093-2711987 (Counterclockwise)

Preceding gene: 119945890

Following gene: 119945888

Centisome position: 59.5

GC content: 40.56

Gene sequence:

>1107_bases
ATGACAACATATAACATTGCATTACTGGCAGGTGACGGTATTGGTCCGGAAGTAATGAAGGAAGCTGTTAAAGTATTAAA
ACTGATTGAACAGCGTAACGAAGACGTTAATTTTGAACTTAACGATGTTTTATTCGGTGCAGCTGCTTATTTTGCAATGG
GTCATGCTTTTCCGGATGAAACTAAAGCGGCTTGTGATAAAGCGGATGCGATTCTAAAAGGGACAATTGGCTTAAATCAC
GAAGATTCTAAAAAAATCCCTATTGATGAACAACCTGAACGTGGTGCATTATTACCGCTGCGTCGTCGTTACAATACCTT
TGCTAACTTCCGTCCGGTTTACTTACCAAAAGGTCTGGCACATTTTTCGCCATTAAAAGCCTCTGTTATTGGTGAAGGTA
TTGATATTATGATCATCCGTGAATTAGTGGGTGGTTTATACTTTGGCGAAAAAGAGATGGGTGTTAATGCAGACGGTAAA
CGTTTTGTACGTGAAGTCCTTGAATATGACGAAGATCAGATCCGTCAGATAGTCAAAGTCGGCTTTGAAGTATCAATGAA
ACGTAAAAAAGTGATGCATAATATTCACAAATCAAACGTTTTAAAGTCAAGCGTGCTGTGGAATGAAATTGTTGAAGAAG
AGAGCAAAAACTATCCAGAAGTTGAAGTTAAAAATATTCTGGTTGATGCTGCTGCTACCTACCTTTGTCTAAACCCCGGT
ATGTTTGATGTAATGGTCATGGAAAATATGTTTGGTGATATTTTAAGTGATCAGGGGGGCGGTATTTTGGGCTCTTTAGG
CTTAATGCCATCGGCGTGTAAAGGTCCTGACAAATCTTATTATGAACCTTCACACGGCAGTGCACCAGATATCGCAGGCA
AAGGTATTGCAAACCCCTATTCAATGATAGGTTCAGTTGCACTGATGCTGGAAATGAGTTTTGGGTTGGAGAAAGAGGCG
AAAAACCTTTGGGCTGCGATGCAGGCTGTCTTTGAAAACGGTTATTCAACATCGGATCTTTCTAAAGAAGGCGAAGCTAA
TCTTGTTAGTACCGCTGAATTTGGTGATCTGGTCTGTGCAGAACTGGCTAAGATGCCCGTTGCATAA

Upstream 100 bases:

>100_bases
AAACAAATTTAACTAAAAAAGCCCTCCTAGGTTTGAAACTAGGGGGGCTTTTACTTATGATACTTTACAAAAGAAGACAT
TCATTATTTAGGGAAATAAA

Downstream 100 bases:

>100_bases
ATAAACTGCAAAATAAAAAATAAAAAGCCACGTTGAAACGTGGCTTTTTTCTTTAAATTGGAAAAATAATGGCACTGAAA
CCGACAATTTTTAAAATGAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTTYNIALLAGDGIGPEVMKEAVKVLKLIEQRNEDVNFELNDVLFGAAAYFAMGHAFPDETKAACDKADAILKGTIGLNH
EDSKKIPIDEQPERGALLPLRRRYNTFANFRPVYLPKGLAHFSPLKASVIGEGIDIMIIRELVGGLYFGEKEMGVNADGK
RFVREVLEYDEDQIRQIVKVGFEVSMKRKKVMHNIHKSNVLKSSVLWNEIVEEESKNYPEVEVKNILVDAAATYLCLNPG
MFDVMVMENMFGDILSDQGGGILGSLGLMPSACKGPDKSYYEPSHGSAPDIAGKGIANPYSMIGSVALMLEMSFGLEKEA
KNLWAAMQAVFENGYSTSDLSKEGEANLVSTAEFGDLVCAELAKMPVA

Sequences:

>Translated_368_residues
MTTYNIALLAGDGIGPEVMKEAVKVLKLIEQRNEDVNFELNDVLFGAAAYFAMGHAFPDETKAACDKADAILKGTIGLNH
EDSKKIPIDEQPERGALLPLRRRYNTFANFRPVYLPKGLAHFSPLKASVIGEGIDIMIIRELVGGLYFGEKEMGVNADGK
RFVREVLEYDEDQIRQIVKVGFEVSMKRKKVMHNIHKSNVLKSSVLWNEIVEEESKNYPEVEVKNILVDAAATYLCLNPG
MFDVMVMENMFGDILSDQGGGILGSLGLMPSACKGPDKSYYEPSHGSAPDIAGKGIANPYSMIGSVALMLEMSFGLEKEA
KNLWAAMQAVFENGYSTSDLSKEGEANLVSTAEFGDLVCAELAKMPVA
>Mature_367_residues
TTYNIALLAGDGIGPEVMKEAVKVLKLIEQRNEDVNFELNDVLFGAAAYFAMGHAFPDETKAACDKADAILKGTIGLNHE
DSKKIPIDEQPERGALLPLRRRYNTFANFRPVYLPKGLAHFSPLKASVIGEGIDIMIIRELVGGLYFGEKEMGVNADGKR
FVREVLEYDEDQIRQIVKVGFEVSMKRKKVMHNIHKSNVLKSSVLWNEIVEEESKNYPEVEVKNILVDAAATYLCLNPGM
FDVMVMENMFGDILSDQGGGILGSLGLMPSACKGPDKSYYEPSHGSAPDIAGKGIANPYSMIGSVALMLEMSFGLEKEAK
NLWAAMQAVFENGYSTSDLSKEGEANLVSTAEFGDLVCAELAKMPVA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=367, Percent_Identity=28.0653950953678, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=26.1363636363636, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI28178816, Length=324, Percent_Identity=26.5432098765432, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI28178821, Length=324, Percent_Identity=26.5432098765432, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI28178838, Length=340, Percent_Identity=26.1764705882353, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=30.9859154929577, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=42.4657534246575, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=32.2493224932249, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787381, Length=307, Percent_Identity=24.4299674267101, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71986051, Length=368, Percent_Identity=27.9891304347826, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=323, Percent_Identity=29.1021671826625, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=27.7008310249307, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17505779, Length=361, Percent_Identity=27.4238227146814, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=37.466307277628, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=371, Percent_Identity=30.7277628032345, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=370, Percent_Identity=26.4864864864865, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24643270, Length=367, Percent_Identity=30.5177111716621, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643268, Length=367, Percent_Identity=30.5177111716621, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=30.0578034682081, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078633, Length=340, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24650122, Length=340, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161078635, Length=340, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161078637, Length=340, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI161078639, Length=212, Percent_Identity=30.6603773584906, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362242, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648872, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 40304; Mature: 40173

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYNIALLAGDGIGPEVMKEAVKVLKLIEQRNEDVNFELNDVLFGAAAYFAMGHAFPDE
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCH
TKAACDKADAILKGTIGLNHEDSKKIPIDEQPERGALLPLRRRYNTFANFRPVYLPKGLA
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHH
HFSPLKASVIGEGIDIMIIRELVGGLYFGEKEMGVNADGKRFVREVLEYDEDQIRQIVKV
HHCCHHHHHHCCCCHHHHHHHHHHHHEECCHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
GFEVSMKRKKVMHNIHKSNVLKSSVLWNEIVEEESKNYPEVEVKNILVDAAATYLCLNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCC
MFDVMVMENMFGDILSDQGGGILGSLGLMPSACKGPDKSYYEPSHGSAPDIAGKGIANPY
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SMIGSVALMLEMSFGLEKEAKNLWAAMQAVFENGYSTSDLSKEGEANLVSTAEFGDLVCA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHH
ELAKMPVA
HHHHCCCC
>Mature Secondary Structure 
TTYNIALLAGDGIGPEVMKEAVKVLKLIEQRNEDVNFELNDVLFGAAAYFAMGHAFPDE
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCH
TKAACDKADAILKGTIGLNHEDSKKIPIDEQPERGALLPLRRRYNTFANFRPVYLPKGLA
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHH
HFSPLKASVIGEGIDIMIIRELVGGLYFGEKEMGVNADGKRFVREVLEYDEDQIRQIVKV
HHCCHHHHHHCCCCHHHHHHHHHHHHEECCHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
GFEVSMKRKKVMHNIHKSNVLKSSVLWNEIVEEESKNYPEVEVKNILVDAAATYLCLNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCC
MFDVMVMENMFGDILSDQGGGILGSLGLMPSACKGPDKSYYEPSHGSAPDIAGKGIANPY
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SMIGSVALMLEMSFGLEKEAKNLWAAMQAVFENGYSTSDLSKEGEANLVSTAEFGDLVCA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHH
ELAKMPVA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA