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The map label for this gene is purK [H]

Identifier: 119945723

GI number: 119945723

Start: 2494489

End: 2495619

Strand: Direct

Name: purK [H]

Synonym: Ping_2049

Alternate gene names: 119945723

Gene position: 2494489-2495619 (Clockwise)

Preceding gene: 119945722

Following gene: 119945724

Centisome position: 54.71

GC content: 42.0

Gene sequence:

>1131_bases
ATGCGAATCGCCATTGTCGGTTGTGGCCAGCTCTCTCGTATGATGGCCCTCGCGGGGCTGCCTCTGGGCATTCAATTTAG
CTTTATTCACGATGATAAAAGCCAAAACAGAGACTGTGTACAAGGCTTAGGCATCATAGAATATTCGCCTGACAAAATAA
AAAGCACAAACAGCTATGACACTAAAGCCATCGCACAACTTTACCAAGCTCTAGGTCAACCTGATTGTATTAGCGTTGAA
AAAGAGCAAGTTGACCTTGCTTTATTACAAGCATTAGCTGATTTTTGTCCTATTTATCCGTGTGTTGCAGCGATAAAAGC
TTGCCAACACCGAGGTGAAGAAAAACAGTTATTAACTGACTTAGCTATTCCAACTTCTCCTTATTTTTATAATAACGGTG
CTAAGCAAGCGGTTCAGACTTTAGGTTATCCCGTTATGGCCAAATCATGCACCGAAGGCTACGACGGCAAAAACCAATGG
CTGATTAAAACCCCTTCTGATGCAGTGCAGTTTGATAAGTTACAAATTGAAGATTATATCATTGAAAAGTTTATCGAATT
TGATAAAGAGATATCACTTATCTCAGTGCGCAGCCAAAGTGGCCAAATCAAGCACTACTCTCTCACCGAGAATCATCATC
AGCAGGGAATGTTAACACACTCAATCGCGCCCGCTCTTAATATTCCTGACTCTGTAAAGCTGCAGGCACAACATTATATG
GAAAGCCTGCTCAACCAATTAGAGTATGTCGGCATACTGGCCATCGAGTTTTTTGTGGTTGGCGATAGCCTGATGGTTAA
CGAACTGGCTCCTCGCGTTCACAACAGTGGGCACTGGACTCAATTAGGCAGTATTACTTGCCAGTTTGAAAATCACATTA
GGGCCTTAGCTGGACTTAATCTAGGCAGTACAGAACAGATAGCGGTGACTGGCATGTTCAACCTTATCGGCACCAAGCAG
CCCCCCTTTGATGCATTAACCCAAAACAGCAAACTATATTGGTATAACAAAGCGCCTAAAGCAAAAAGGAAATTAGGGCA
CATTAATTTTATTGCTGAAAATCAACAATTGGTTAAACAACAGATGATCCAACTAGCTGATGCTAATCTATCACTGACTG
TTCATCATTAA

Upstream 100 bases:

>100_bases
TAGCGGCTCAGTTTCTGGCAATCCACGATTCAAAATTGCAAAGCAAAGTAAAAGCATGGCGCCAGCAGCAAACTGAGAGC
ATTGCTTGGGAGGTGGAATA

Downstream 100 bases:

>100_bases
CTGATGTCTAACGATTAAAAAATAATAACGAATGCTGCAGCCTATTTATGCAGTTTTTTATTTGGAGAATAATGTCGTGC
TTGGAAATCGTTTTGAAAAT

Product: phosphoribosylaminoimidazole carboxylase

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRIAIVGCGQLSRMMALAGLPLGIQFSFIHDDKSQNRDCVQGLGIIEYSPDKIKSTNSYDTKAIAQLYQALGQPDCISVE
KEQVDLALLQALADFCPIYPCVAAIKACQHRGEEKQLLTDLAIPTSPYFYNNGAKQAVQTLGYPVMAKSCTEGYDGKNQW
LIKTPSDAVQFDKLQIEDYIIEKFIEFDKEISLISVRSQSGQIKHYSLTENHHQQGMLTHSIAPALNIPDSVKLQAQHYM
ESLLNQLEYVGILAIEFFVVGDSLMVNELAPRVHNSGHWTQLGSITCQFENHIRALAGLNLGSTEQIAVTGMFNLIGTKQ
PPFDALTQNSKLYWYNKAPKAKRKLGHINFIAENQQLVKQQMIQLADANLSLTVHH

Sequences:

>Translated_376_residues
MRIAIVGCGQLSRMMALAGLPLGIQFSFIHDDKSQNRDCVQGLGIIEYSPDKIKSTNSYDTKAIAQLYQALGQPDCISVE
KEQVDLALLQALADFCPIYPCVAAIKACQHRGEEKQLLTDLAIPTSPYFYNNGAKQAVQTLGYPVMAKSCTEGYDGKNQW
LIKTPSDAVQFDKLQIEDYIIEKFIEFDKEISLISVRSQSGQIKHYSLTENHHQQGMLTHSIAPALNIPDSVKLQAQHYM
ESLLNQLEYVGILAIEFFVVGDSLMVNELAPRVHNSGHWTQLGSITCQFENHIRALAGLNLGSTEQIAVTGMFNLIGTKQ
PPFDALTQNSKLYWYNKAPKAKRKLGHINFIAENQQLVKQQMIQLADANLSLTVHH
>Mature_376_residues
MRIAIVGCGQLSRMMALAGLPLGIQFSFIHDDKSQNRDCVQGLGIIEYSPDKIKSTNSYDTKAIAQLYQALGQPDCISVE
KEQVDLALLQALADFCPIYPCVAAIKACQHRGEEKQLLTDLAIPTSPYFYNNGAKQAVQTLGYPVMAKSCTEGYDGKNQW
LIKTPSDAVQFDKLQIEDYIIEKFIEFDKEISLISVRSQSGQIKHYSLTENHHQQGMLTHSIAPALNIPDSVKLQAQHYM
ESLLNQLEYVGILAIEFFVVGDSLMVNELAPRVHNSGHWTQLGSITCQFENHIRALAGLNLGSTEQIAVTGMFNLIGTKQ
PPFDALTQNSKLYWYNKAPKAKRKLGHINFIAENQQLVKQQMIQLADANLSLTVHH

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=357, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788155, Length=319, Percent_Identity=25.705329153605, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=29.3814432989691, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41938; Mature: 41938

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAIVGCGQLSRMMALAGLPLGIQFSFIHDDKSQNRDCVQGLGIIEYSPDKIKSTNSYD
CEEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCEEEECCHHCCCCCCCC
TKAIAQLYQALGQPDCISVEKEQVDLALLQALADFCPIYPCVAAIKACQHRGEEKQLLTD
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
LAIPTSPYFYNNGAKQAVQTLGYPVMAKSCTEGYDGKNQWLIKTPSDAVQFDKLQIEDYI
HCCCCCCCEECCCHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
IEKFIEFDKEISLISVRSQSGQIKHYSLTENHHQQGMLTHSIAPALNIPDSVKLQAQHYM
HHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
ESLLNQLEYVGILAIEFFVVGDSLMVNELAPRVHNSGHWTQLGSITCQFENHIRALAGLN
HHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHCCC
LGSTEQIAVTGMFNLIGTKQPPFDALTQNSKLYWYNKAPKAKRKLGHINFIAENQQLVKQ
CCCCCEEEEEEHHHHHCCCCCCHHHHCCCCEEEEECCCCHHHHHCCCEEEEECCHHHHHH
QMIQLADANLSLTVHH
HHHHHHCCCEEEEEEC
>Mature Secondary Structure
MRIAIVGCGQLSRMMALAGLPLGIQFSFIHDDKSQNRDCVQGLGIIEYSPDKIKSTNSYD
CEEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCEEEECCHHCCCCCCCC
TKAIAQLYQALGQPDCISVEKEQVDLALLQALADFCPIYPCVAAIKACQHRGEEKQLLTD
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
LAIPTSPYFYNNGAKQAVQTLGYPVMAKSCTEGYDGKNQWLIKTPSDAVQFDKLQIEDYI
HCCCCCCCEECCCHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
IEKFIEFDKEISLISVRSQSGQIKHYSLTENHHQQGMLTHSIAPALNIPDSVKLQAQHYM
HHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
ESLLNQLEYVGILAIEFFVVGDSLMVNELAPRVHNSGHWTQLGSITCQFENHIRALAGLN
HHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHCCC
LGSTEQIAVTGMFNLIGTKQPPFDALTQNSKLYWYNKAPKAKRKLGHINFIAENQQLVKQ
CCCCCEEEEEEHHHHHCCCCCCHHHHCCCCEEEEECCCCHHHHHCCCEEEEECCHHHHHH
QMIQLADANLSLTVHH
HHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]