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The map label for this gene is ybjS [C]

Identifier: 119945684

GI number: 119945684

Start: 2432193

End: 2433266

Strand: Direct

Name: ybjS [C]

Synonym: Ping_2000

Alternate gene names: 119945684

Gene position: 2432193-2433266 (Clockwise)

Preceding gene: 119945683

Following gene: 119945685

Centisome position: 53.34

GC content: 43.3

Gene sequence:

>1074_bases
ATGCCAGTCAATAAAATTCCAACATTCAGCCACTTGAATGACCAACAACAGCTGGTTTTAAGGCGTTTATCGATACAGGT
TAAAAAGGTATTTGTGACTGGTGCAGGTGGTTTTTTAGGTAAAGCATTGTGCCGTTTTTTACGCTCTGCTGATATTAATG
TTATTGGCTTTGCACGTGGAGATTACCCCGAGCTTGAGCAAATGGGCGTGACCATGGTTAAAGGTGACATATCTGACAAA
AAATCTCTTTTTGATGCCATGAAAGGCACTGATTTGGTCTTTCATGTCGCCTCGAAAGCCGGAGTCTGGGGCACTATGGA
GTCTTATTTTAGTGCCAATATTAAAGGGACAGAAAATATTATCGGTGTCTGCCAAGACCTTAATATAAAGCGCTTGGTTT
ATACCAGCACACCCAGTGTGACCTTTGCGGGGAAAGATGAAAACGAAATCGACGAATCTCAGCCCTACGCTGATAATTTT
TTAAACTTTTATGCTTTATCTAAAGCGATTGCAGAAGCCGAAATCCTAGGCTCAAATTCAGCAGCGCTTAAAACGGTTGC
TCTTCGCCCCCACCTTATTTGGGGACCGGGCGATCCGCACCTTGTGCCTCGCGTATTACAACGGGCCAAATCGGGACGGC
TTAAACTGGTGGGTAAAACAGACAAGCTGGTCGATACTACCTATATTGATAACGCGGTTTACGCGCATCTTTTAGCGGCT
GTTAACCTAAGTAAAGCAAACCCAAACTGTGCAGGAAAAGCATATTTTGTCAGTAACGATCAACCAATATTGATGGCAGA
AATGCTTAATAAAATTCTTGCTTGCCAAGGTTTAAAACCAATCGACGCACGTATTCCGGCGCCGCTTGCTTATGTTATTG
GTGCCACACTGGAGTGGGTTTATTTGTATTTGAACATTAAAAAAGAGCCCCCTATCACTCGTTTTGTCGCACGTCAGCTG
TCAACTAGCCATTATTTCAATATCAGCGCCGCTAAAAATGATATTGGTTATCAACCTATTGTGAGTATCGATCAGGGCAT
GGAAAAACTGAAAGAGTCACTGGCGGAGAGCTGA

Upstream 100 bases:

>100_bases
ATAAAAAATTCCCCATGGATATTCGCCATAACGCAAAAATATACCGTGAAAAATTAGCCATTTGGGCAGAAAAACAACTA
ATAGATTAAGCAGAAGCCTT

Downstream 100 bases:

>100_bases
AGACTATCGGCGATCGGCTGTCGGCTGTCGGCTGTCGGCGATCAGCTGTGGGCTTTCGGCTGCGGGCTGCGAGAAGGCGA
GCTGCGGGCTACGAGTGGCG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPVNKIPTFSHLNDQQQLVLRRLSIQVKKVFVTGAGGFLGKALCRFLRSADINVIGFARGDYPELEQMGVTMVKGDISDK
KSLFDAMKGTDLVFHVASKAGVWGTMESYFSANIKGTENIIGVCQDLNIKRLVYTSTPSVTFAGKDENEIDESQPYADNF
LNFYALSKAIAEAEILGSNSAALKTVALRPHLIWGPGDPHLVPRVLQRAKSGRLKLVGKTDKLVDTTYIDNAVYAHLLAA
VNLSKANPNCAGKAYFVSNDQPILMAEMLNKILACQGLKPIDARIPAPLAYVIGATLEWVYLYLNIKKEPPITRFVARQL
STSHYFNISAAKNDIGYQPIVSIDQGMEKLKESLAES

Sequences:

>Translated_357_residues
MPVNKIPTFSHLNDQQQLVLRRLSIQVKKVFVTGAGGFLGKALCRFLRSADINVIGFARGDYPELEQMGVTMVKGDISDK
KSLFDAMKGTDLVFHVASKAGVWGTMESYFSANIKGTENIIGVCQDLNIKRLVYTSTPSVTFAGKDENEIDESQPYADNF
LNFYALSKAIAEAEILGSNSAALKTVALRPHLIWGPGDPHLVPRVLQRAKSGRLKLVGKTDKLVDTTYIDNAVYAHLLAA
VNLSKANPNCAGKAYFVSNDQPILMAEMLNKILACQGLKPIDARIPAPLAYVIGATLEWVYLYLNIKKEPPITRFVARQL
STSHYFNISAAKNDIGYQPIVSIDQGMEKLKESLAES
>Mature_356_residues
PVNKIPTFSHLNDQQQLVLRRLSIQVKKVFVTGAGGFLGKALCRFLRSADINVIGFARGDYPELEQMGVTMVKGDISDKK
SLFDAMKGTDLVFHVASKAGVWGTMESYFSANIKGTENIIGVCQDLNIKRLVYTSTPSVTFAGKDENEIDESQPYADNFL
NFYALSKAIAEAEILGSNSAALKTVALRPHLIWGPGDPHLVPRVLQRAKSGRLKLVGKTDKLVDTTYIDNAVYAHLLAAV
NLSKANPNCAGKAYFVSNDQPILMAEMLNKILACQGLKPIDARIPAPLAYVIGATLEWVYLYLNIKKEPPITRFVARQLS
TSHYFNISAAKNDIGYQPIVSIDQGMEKLKESLAES

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=337, Percent_Identity=33.8278931750742, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI8393516, Length=337, Percent_Identity=33.8278931750742, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI116268111, Length=332, Percent_Identity=31.9277108433735, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI310132178, Length=344, Percent_Identity=32.8488372093023, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310113012, Length=344, Percent_Identity=32.8488372093023, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI239745448, Length=331, Percent_Identity=33.5347432024169, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI260763931, Length=353, Percent_Identity=31.1614730878187, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4504509, Length=353, Percent_Identity=31.1614730878187, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4504507, Length=355, Percent_Identity=31.2676056338028, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI19923621, Length=354, Percent_Identity=27.1186440677966, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310113808, Length=331, Percent_Identity=26.5861027190332, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310127990, Length=331, Percent_Identity=26.2839879154079, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081792, Length=332, Percent_Identity=29.2168674698795, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17570557, Length=354, Percent_Identity=24.5762711864407, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193211272, Length=327, Percent_Identity=24.1590214067278, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17509709, Length=287, Percent_Identity=24.0418118466899, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321437, Length=345, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21355349, Length=284, Percent_Identity=28.8732394366197, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 39214; Mature: 39083

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNKIPTFSHLNDQQQLVLRRLSIQVKKVFVTGAGGFLGKALCRFLRSADINVIGFARG
CCCCCCCCHHHCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCC
DYPELEQMGVTMVKGDISDKKSLFDAMKGTDLVFHVASKAGVWGTMESYFSANIKGTENI
CCCCHHHCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHH
IGVCQDLNIKRLVYTSTPSVTFAGKDENEIDESQPYADNFLNFYALSKAIAEAEILGSNS
HHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AALKTVALRPHLIWGPGDPHLVPRVLQRAKSGRLKLVGKTDKLVDTTYIDNAVYAHLLAA
HHEEEEEECCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHH
VNLSKANPNCAGKAYFVSNDQPILMAEMLNKILACQGLKPIDARIPAPLAYVIGATLEWV
HHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEE
YLYLNIKKEPPITRFVARQLSTSHYFNISAAKNDIGYQPIVSIDQGMEKLKESLAES
EEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVNKIPTFSHLNDQQQLVLRRLSIQVKKVFVTGAGGFLGKALCRFLRSADINVIGFARG
CCCCCCCHHHCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCC
DYPELEQMGVTMVKGDISDKKSLFDAMKGTDLVFHVASKAGVWGTMESYFSANIKGTENI
CCCCHHHCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHH
IGVCQDLNIKRLVYTSTPSVTFAGKDENEIDESQPYADNFLNFYALSKAIAEAEILGSNS
HHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AALKTVALRPHLIWGPGDPHLVPRVLQRAKSGRLKLVGKTDKLVDTTYIDNAVYAHLLAA
HHEEEEEECCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHH
VNLSKANPNCAGKAYFVSNDQPILMAEMLNKILACQGLKPIDARIPAPLAYVIGATLEWV
HHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEE
YLYLNIKKEPPITRFVARQLSTSHYFNISAAKNDIGYQPIVSIDQGMEKLKESLAES
EEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]