Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 119945683

GI number: 119945683

Start: 2430517

End: 2432181

Strand: Direct

Name: lcfB [H]

Synonym: Ping_1999

Alternate gene names: 119945683

Gene position: 2430517-2432181 (Clockwise)

Preceding gene: 119945682

Following gene: 119945684

Centisome position: 53.31

GC content: 42.16

Gene sequence:

>1665_bases
ATGTCCGATCATCAATACTGCAACTTTTGCCGTCATTTAATCGATGCGGCAACGGCTTTCCCTGACTCCCTAGCAGTTGC
GGTTCAAAAAAAACGATTTAATAAGCTGACTTATCAGGAGATAGATTTTTCCACCCTGAATAAACGCAGTGATCGAATAG
CCCATGCACTGAATCAATTTGGCTTAAAAAGTGGCGATAAAGCGGTCTTAATGGTCAAACCCAGTGTAGACTTTTTTGCA
TTAACCTTTGCACTGTTCAAAGCGGGAATAGTACCGATATTAATAGATCCTGGAATGGGCATTAAAAACCTCAAACAGTG
TATCGCAGAAGTACATCCGGACGCTTTTATTGGTATTCCCAAAGCACATATTGCCCGGCGACTATTCGGCTGGGGCAAAG
ACGCCATTAAATACAATATTACCGTAGGGGGGGCCAGTTTTTGGGGAGGTACTAGCCTAACTCAAATAATTGATCATTGC
AGCGCACAAACCCCCTACCCCATGGTTAAACTTACACCGGATCAAATGGCGGCCATTTTATTTACCAGCGGCAGTACGGG
CTCACCTAAAGGGGTTGTTTATACCCACCGAATGTTTGAAGCGCAAATCACCGTCTTAAGAAATGATTATGGGATAACCC
ATGGAGAACGGGACTTAGCGACCTTTCCTTTGTTTTCACTTTTTGGTCCGGCACTGGGTATGGCATCAATTATTCCAGCA
ATGGATGCCAGTAAACCGATAACCGCCAATCCCGATTATATTTTTGCTGCCATTAACAAATATCACTGTACCAACCTTTT
TGCCAATCCGGCATTAATAGAGTTATTAGGCCGTGCGGGTAAGTCACGCGCGATGAAACTGCAAAGTCTGCAGCGCGTTA
TCTCAGCGGGTGCACCCGCAACCTTGTCTTCCATTGAACGGTTCACTACGTTACTTAACCCAGGGGTAGAAATTCTCACT
TCTTACGGTGCAACCGAAGCCTTACCAGTCAGTAAAATTGGCAGCGTAGCCTTACTCAAAACAACAGACATCACCGATGC
AGGCGGCGGGATTTGTGTTGGTTATCCCGTTGATGGTATGACTGTTTCTATTATTAACATCACCGAAAAAACCATCACAA
CCTGGCAAGATGATCTTAGATTGCCGGCCTATGAAATCGGTGAGATTGTGGTTCAGGGCCCACAAGTAAGCCAGTGCTAT
TATCAACGAAAAAATGCTACACTTGCCGCTAAAATAGAAGATATTAAAAGACCACACCACCGTATGGGTGATTTAGGTTA
TCTCGATTCCGATGGGCAATTATGGATGTGTGGCCGAAAAGCACATCGTGTTGATGCCAATCATCTTAAAATTAAAAAAA
GTTATTATTCCATTCCCTGTGAACGTATTTTTAATACTCATCCAGCCATTAAACGCAGTGCATTGGTTGCTGTCAAAATA
GATGGAAAAACAACCCCTTTATTATGTATTGAGCTGTATAATAAATCTGCACAAACAGCTGAAACGTTATTTGCAGAGTT
GCATAAAATAGCCGATAAGCATAATCAAACAGTAGGTATTACACTGTTTTTAATTCATAAAAAATTCCCCATGGATATTC
GCCATAACGCAAAAATATACCGTGAAAAATTAGCCATTTGGGCAGAAAAACAACTAATAGATTAA

Upstream 100 bases:

>100_bases
GGTTCATGAGTTTGACGATTGTGGTCATTATATTCTGGAAGATGCCAGTGAACAGGTGATCCCGCTTATTGAAACATTTA
TGGCAGAAAACCCTTAACTT

Downstream 100 bases:

>100_bases
GCAGAAGCCTTATGCCAGTCAATAAAATTCCAACATTCAGCCACTTGAATGACCAACAACAGCTGGTTTTAAGGCGTTTA
TCGATACAGGTTAAAAAGGT

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSDHQYCNFCRHLIDAATAFPDSLAVAVQKKRFNKLTYQEIDFSTLNKRSDRIAHALNQFGLKSGDKAVLMVKPSVDFFA
LTFALFKAGIVPILIDPGMGIKNLKQCIAEVHPDAFIGIPKAHIARRLFGWGKDAIKYNITVGGASFWGGTSLTQIIDHC
SAQTPYPMVKLTPDQMAAILFTSGSTGSPKGVVYTHRMFEAQITVLRNDYGITHGERDLATFPLFSLFGPALGMASIIPA
MDASKPITANPDYIFAAINKYHCTNLFANPALIELLGRAGKSRAMKLQSLQRVISAGAPATLSSIERFTTLLNPGVEILT
SYGATEALPVSKIGSVALLKTTDITDAGGGICVGYPVDGMTVSIINITEKTITTWQDDLRLPAYEIGEIVVQGPQVSQCY
YQRKNATLAAKIEDIKRPHHRMGDLGYLDSDGQLWMCGRKAHRVDANHLKIKKSYYSIPCERIFNTHPAIKRSALVAVKI
DGKTTPLLCIELYNKSAQTAETLFAELHKIADKHNQTVGITLFLIHKKFPMDIRHNAKIYREKLAIWAEKQLID

Sequences:

>Translated_554_residues
MSDHQYCNFCRHLIDAATAFPDSLAVAVQKKRFNKLTYQEIDFSTLNKRSDRIAHALNQFGLKSGDKAVLMVKPSVDFFA
LTFALFKAGIVPILIDPGMGIKNLKQCIAEVHPDAFIGIPKAHIARRLFGWGKDAIKYNITVGGASFWGGTSLTQIIDHC
SAQTPYPMVKLTPDQMAAILFTSGSTGSPKGVVYTHRMFEAQITVLRNDYGITHGERDLATFPLFSLFGPALGMASIIPA
MDASKPITANPDYIFAAINKYHCTNLFANPALIELLGRAGKSRAMKLQSLQRVISAGAPATLSSIERFTTLLNPGVEILT
SYGATEALPVSKIGSVALLKTTDITDAGGGICVGYPVDGMTVSIINITEKTITTWQDDLRLPAYEIGEIVVQGPQVSQCY
YQRKNATLAAKIEDIKRPHHRMGDLGYLDSDGQLWMCGRKAHRVDANHLKIKKSYYSIPCERIFNTHPAIKRSALVAVKI
DGKTTPLLCIELYNKSAQTAETLFAELHKIADKHNQTVGITLFLIHKKFPMDIRHNAKIYREKLAIWAEKQLID
>Mature_553_residues
SDHQYCNFCRHLIDAATAFPDSLAVAVQKKRFNKLTYQEIDFSTLNKRSDRIAHALNQFGLKSGDKAVLMVKPSVDFFAL
TFALFKAGIVPILIDPGMGIKNLKQCIAEVHPDAFIGIPKAHIARRLFGWGKDAIKYNITVGGASFWGGTSLTQIIDHCS
AQTPYPMVKLTPDQMAAILFTSGSTGSPKGVVYTHRMFEAQITVLRNDYGITHGERDLATFPLFSLFGPALGMASIIPAM
DASKPITANPDYIFAAINKYHCTNLFANPALIELLGRAGKSRAMKLQSLQRVISAGAPATLSSIERFTTLLNPGVEILTS
YGATEALPVSKIGSVALLKTTDITDAGGGICVGYPVDGMTVSIINITEKTITTWQDDLRLPAYEIGEIVVQGPQVSQCYY
QRKNATLAAKIEDIKRPHHRMGDLGYLDSDGQLWMCGRKAHRVDANHLKIKKSYYSIPCERIFNTHPAIKRSALVAVKID
GKTTPLLCIELYNKSAQTAETLFAELHKIADKHNQTVGITLFLIHKKFPMDIRHNAKIYREKLAIWAEKQLID

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=468, Percent_Identity=23.9316239316239, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693145, Length=479, Percent_Identity=24.8434237995825, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=539, Percent_Identity=21.3358070500928, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=539, Percent_Identity=21.3358070500928, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=539, Percent_Identity=21.3358070500928, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=512, Percent_Identity=22.4609375, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922652, Length=492, Percent_Identity=23.5772357723577, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61083; Mature: 60951

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHQYCNFCRHLIDAATAFPDSLAVAVQKKRFNKLTYQEIDFSTLNKRSDRIAHALNQF
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHH
GLKSGDKAVLMVKPSVDFFALTFALFKAGIVPILIDPGMGIKNLKQCIAEVHPDAFIGIP
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEECCC
KAHIARRLFGWGKDAIKYNITVGGASFWGGTSLTQIIDHCSAQTPYPMVKLTPDQMAAIL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECHHHEEEEE
FTSGSTGSPKGVVYTHRMFEAQITVLRNDYGITHGERDLATFPLFSLFGPALGMASIIPA
EECCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
MDASKPITANPDYIFAAINKYHCTNLFANPALIELLGRAGKSRAMKLQSLQRVISAGAPA
CCCCCCCCCCCCEEEEEECHHEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
TLSSIERFTTLLNPGVEILTSYGATEALPVSKIGSVALLKTTDITDAGGGICVGYPVDGM
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCCCCCEEEEECCCCE
TVSIINITEKTITTWQDDLRLPAYEIGEIVVQGPQVSQCYYQRKNATLAAKIEDIKRPHH
EEEEEEECHHHHHHHHHHCCCCHHHHCCEEECCCCHHHHHHHHCCCEEEEHHHHHCCHHH
RMGDLGYLDSDGQLWMCGRKAHRVDANHLKIKKSYYSIPCERIFNTHPAIKRSALVAVKI
HCCCCCEECCCCCEEEECCHHHCCCCCCEEEEHHHHCCCHHHHHCCCCCCCCCEEEEEEE
DGKTTPLLCIELYNKSAQTAETLFAELHKIADKHNQTVGITLFLIHKKFPMDIRHNAKIY
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCHHHH
REKLAIWAEKQLID
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SDHQYCNFCRHLIDAATAFPDSLAVAVQKKRFNKLTYQEIDFSTLNKRSDRIAHALNQF
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHH
GLKSGDKAVLMVKPSVDFFALTFALFKAGIVPILIDPGMGIKNLKQCIAEVHPDAFIGIP
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEECCC
KAHIARRLFGWGKDAIKYNITVGGASFWGGTSLTQIIDHCSAQTPYPMVKLTPDQMAAIL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECHHHEEEEE
FTSGSTGSPKGVVYTHRMFEAQITVLRNDYGITHGERDLATFPLFSLFGPALGMASIIPA
EECCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
MDASKPITANPDYIFAAINKYHCTNLFANPALIELLGRAGKSRAMKLQSLQRVISAGAPA
CCCCCCCCCCCCEEEEEECHHEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
TLSSIERFTTLLNPGVEILTSYGATEALPVSKIGSVALLKTTDITDAGGGICVGYPVDGM
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCCCCCEEEEECCCCE
TVSIINITEKTITTWQDDLRLPAYEIGEIVVQGPQVSQCYYQRKNATLAAKIEDIKRPHH
EEEEEEECHHHHHHHHHHCCCCHHHHCCEEECCCCHHHHHHHHCCCEEEEHHHHHCCHHH
RMGDLGYLDSDGQLWMCGRKAHRVDANHLKIKKSYYSIPCERIFNTHPAIKRSALVAVKI
HCCCCCEECCCCCEEEECCHHHCCCCCCEEEEHHHHCCCHHHHHCCCCCCCCCEEEEEEE
DGKTTPLLCIELYNKSAQTAETLFAELHKIADKHNQTVGITLFLIHKKFPMDIRHNAKIY
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCHHHH
REKLAIWAEKQLID
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]