Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 119945381

GI number: 119945381

Start: 2027837

End: 2029234

Strand: Direct

Name: leuC

Synonym: Ping_1669

Alternate gene names: 119945381

Gene position: 2027837-2029234 (Clockwise)

Preceding gene: 119945378

Following gene: 119945382

Centisome position: 44.47

GC content: 42.78

Gene sequence:

>1398_bases
ATGACTAAAACAATGTATGAAAAAATCTGGGATGCGCATTTGGTTTATGAACCAGAGTCAGGCTCTCCAATTCTGTTTGT
TGACCGACATCTAATGCATGAAGTGACTAGTCCACAAGCATTTGAAGGCCTTAAACTGCAAAATCGTGGTGTGCGCAATA
CGCACAGTATATTAGCCACAATGGATCATTGTGTTCCGACAAAAGGTCGTGCCGAAATTTCTGATCCTGTTGCAGCAAAA
CAGATTCAAACGATGGCTGTTAACTGTAAAGAACATGGTATAAACCTTTATGATATGAGCAATGCTAACAACGGTATTAT
TCATATCGTTGTGCCAGAGCATGGTTTTGTTCATCCTGGCATGGTTGTTTGTTGCGGTGATAGCCATACGTCAACACACG
GTGCATTTGGTACACTCGCATTTGGTATCGGTACTTCTGAAGTTGAACATGTAATGGCAACACAAACATTACAACAACAA
AAATCAAAAACCTTGCTTATAAATGTAGAAGGGACACTTTCTAAACATGCAACGGCTAAAGACATTGCATTGGCTATTAT
CGGTAAGACCGGTACTGCTGGCGGTACAGGTTATGTTATTGAATTTGCTGGTGATGCAGTTGTTAACCTGACTATGGAAG
GTCGTATGACCCTCTGTAATATGGCAATAGAAGCGGGCGCTCGTGCAGGTCTTATTGCACCGGATCAAAAAACCTTTGAT
TTCCTTGAAGGCAAAGAGTTTGCACCTAAGGGCAAAGATTGGGATGCGGCTCTTGCTTATTGGAAAACGCTGCCTTCTGA
TAAGGGGGCTGATTTTGATAAAGTCATCAACTTTAAAGCTGAAGATATCGCACCGCAAGTTACCTGGGGGACTTCACCGG
AACAGGTGATCTCAGTGAATGGAATCGTACCGGCACCTACTGATTTTGATGATCCGATTAAAGCACAGGCTTGTAAAAAT
GCCCTTGAATATATGAAAATTAAAGCCGGCCAGAAAATGACCGATGTTAATGTTGATATTGTATTTTTAGGCTCTTGTAC
TAACGGACGTATTGAAGATTTTCGTGCTGCTGCCGAGATGCTCAAAGGCAAAACCATTGCACCCGGTGTTCAGGCCATCG
CAGTCCCTGGTTCTTACCCTGTAAAAGAACAAGCGGAAGCCGAAGGTTTAGATAAGATTTTTCTTGATGCTGGTTGGGAA
TGGCGTGAACCAGGTTGTTCAATGTGTCTTGCTATGAATGGTGATGAATTAACAGAAGGTCAACGTAGTGCTTCTACTTC
AAACCGTAATTTCGAAGGTCGTCAAGGTAAAAATGGACTTACTCACCTTGTTTCGCCTGCAATGGCGGCAGCGGCATCTA
TTGCCGGACACTTTGTAGATATTCGAGATTGGGCATAA

Upstream 100 bases:

>100_bases
CTAAACGGAACTAAACAACCTATATCTGCTAGTGACTGTTATATGGTTTATCTCCTAGATGGTAGGGATTAATAGAAAGC
ATAGAGGTTGAAGGAAAATA

Downstream 100 bases:

>100_bases
AGGGAACAATAATGGAAGCTTATAAAAAACATACTAGCATTGCGGCATTAATGAATCGCAGTAACGTTGATACAGACCAA
ATTATTCCAACGCAGTTCTT

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTKTMYEKIWDAHLVYEPESGSPILFVDRHLMHEVTSPQAFEGLKLQNRGVRNTHSILATMDHCVPTKGRAEISDPVAAK
QIQTMAVNCKEHGINLYDMSNANNGIIHIVVPEHGFVHPGMVVCCGDSHTSTHGAFGTLAFGIGTSEVEHVMATQTLQQQ
KSKTLLINVEGTLSKHATAKDIALAIIGKTGTAGGTGYVIEFAGDAVVNLTMEGRMTLCNMAIEAGARAGLIAPDQKTFD
FLEGKEFAPKGKDWDAALAYWKTLPSDKGADFDKVINFKAEDIAPQVTWGTSPEQVISVNGIVPAPTDFDDPIKAQACKN
ALEYMKIKAGQKMTDVNVDIVFLGSCTNGRIEDFRAAAEMLKGKTIAPGVQAIAVPGSYPVKEQAEAEGLDKIFLDAGWE
WREPGCSMCLAMNGDELTEGQRSASTSNRNFEGRQGKNGLTHLVSPAMAAAASIAGHFVDIRDWA

Sequences:

>Translated_465_residues
MTKTMYEKIWDAHLVYEPESGSPILFVDRHLMHEVTSPQAFEGLKLQNRGVRNTHSILATMDHCVPTKGRAEISDPVAAK
QIQTMAVNCKEHGINLYDMSNANNGIIHIVVPEHGFVHPGMVVCCGDSHTSTHGAFGTLAFGIGTSEVEHVMATQTLQQQ
KSKTLLINVEGTLSKHATAKDIALAIIGKTGTAGGTGYVIEFAGDAVVNLTMEGRMTLCNMAIEAGARAGLIAPDQKTFD
FLEGKEFAPKGKDWDAALAYWKTLPSDKGADFDKVINFKAEDIAPQVTWGTSPEQVISVNGIVPAPTDFDDPIKAQACKN
ALEYMKIKAGQKMTDVNVDIVFLGSCTNGRIEDFRAAAEMLKGKTIAPGVQAIAVPGSYPVKEQAEAEGLDKIFLDAGWE
WREPGCSMCLAMNGDELTEGQRSASTSNRNFEGRQGKNGLTHLVSPAMAAAASIAGHFVDIRDWA
>Mature_464_residues
TKTMYEKIWDAHLVYEPESGSPILFVDRHLMHEVTSPQAFEGLKLQNRGVRNTHSILATMDHCVPTKGRAEISDPVAAKQ
IQTMAVNCKEHGINLYDMSNANNGIIHIVVPEHGFVHPGMVVCCGDSHTSTHGAFGTLAFGIGTSEVEHVMATQTLQQQK
SKTLLINVEGTLSKHATAKDIALAIIGKTGTAGGTGYVIEFAGDAVVNLTMEGRMTLCNMAIEAGARAGLIAPDQKTFDF
LEGKEFAPKGKDWDAALAYWKTLPSDKGADFDKVINFKAEDIAPQVTWGTSPEQVISVNGIVPAPTDFDDPIKAQACKNA
LEYMKIKAGQKMTDVNVDIVFLGSCTNGRIEDFRAAAEMLKGKTIAPGVQAIAVPGSYPVKEQAEAEGLDKIFLDAGWEW
REPGCSMCLAMNGDELTEGQRSASTSNRNFEGRQGKNGLTHLVSPAMAAAASIAGHFVDIRDWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=477, Percent_Identity=26.2054507337526, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=24.4949494949495, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=409, Percent_Identity=26.161369193154, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=62.0689655172414, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI87081781, Length=341, Percent_Identity=25.2199413489736, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.4847645429363, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=481, Percent_Identity=27.6507276507277, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=386, Percent_Identity=27.720207253886, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=27.8125, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=402, Percent_Identity=25.3731343283582, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=56.5957446808511, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322261, Length=483, Percent_Identity=26.9151138716356, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=373, Percent_Identity=30.2949061662198, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=463, Percent_Identity=26.133909287257, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=27.6762402088773, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=27.6762402088773, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161076999, Length=383, Percent_Identity=27.6762402088773, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28571643, Length=479, Percent_Identity=25.2609603340292, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24645686, Length=384, Percent_Identity=27.6041666666667, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=26.1213720316623, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PSYIN (A1SVE7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943061.1
- STRING:   A1SVE7
- GeneID:   4626918
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1669
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1SVE7
- BioCyc:   PING357804:PING_1669-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50077; Mature: 49946

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTMYEKIWDAHLVYEPESGSPILFVDRHLMHEVTSPQAFEGLKLQNRGVRNTHSILAT
CCHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHCCCCCHHCCCEECCCCCCHHHHHHHH
MDHCVPTKGRAEISDPVAAKQIQTMAVNCKEHGINLYDMSNANNGIIHIVVPEHGFVHPG
HHHHCCCCCCCCCCCCHHHHHHHHHEEEHHHHCCEEEEECCCCCCEEEEEECCCCCCCCC
MVVCCGDSHTSTHGAFGTLAFGIGTSEVEHVMATQTLQQQKSKTLLINVEGTLSKHATAK
EEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
DIALAIIGKTGTAGGTGYVIEFAGDAVVNLTMEGRMTLCNMAIEAGARAGLIAPDQKTFD
EEEEEEEECCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHH
FLEGKEFAPKGKDWDAALAYWKTLPSDKGADFDKVINFKAEDIAPQVTWGTSPEQVISVN
HHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHHCCCEEECCCCCCCEEEEC
GIVPAPTDFDDPIKAQACKNALEYMKIKAGQKMTDVNVDIVFLGSCTNGRIEDFRAAAEM
CEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCCHHHHHHHHHH
LKGKTIAPGVQAIAVPGSYPVKEQAEAEGLDKIFLDAGWEWREPGCSMCLAMNGDELTEG
HCCCCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCHHCCC
QRSASTSNRNFEGRQGKNGLTHLVSPAMAAAASIAGHFVDIRDWA
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
TKTMYEKIWDAHLVYEPESGSPILFVDRHLMHEVTSPQAFEGLKLQNRGVRNTHSILAT
CHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHCCCCCHHCCCEECCCCCCHHHHHHHH
MDHCVPTKGRAEISDPVAAKQIQTMAVNCKEHGINLYDMSNANNGIIHIVVPEHGFVHPG
HHHHCCCCCCCCCCCCHHHHHHHHHEEEHHHHCCEEEEECCCCCCEEEEEECCCCCCCCC
MVVCCGDSHTSTHGAFGTLAFGIGTSEVEHVMATQTLQQQKSKTLLINVEGTLSKHATAK
EEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
DIALAIIGKTGTAGGTGYVIEFAGDAVVNLTMEGRMTLCNMAIEAGARAGLIAPDQKTFD
EEEEEEEECCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHHH
FLEGKEFAPKGKDWDAALAYWKTLPSDKGADFDKVINFKAEDIAPQVTWGTSPEQVISVN
HHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHHCCCEEECCCCCCCEEEEC
GIVPAPTDFDDPIKAQACKNALEYMKIKAGQKMTDVNVDIVFLGSCTNGRIEDFRAAAEM
CEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCCHHHHHHHHHH
LKGKTIAPGVQAIAVPGSYPVKEQAEAEGLDKIFLDAGWEWREPGCSMCLAMNGDELTEG
HCCCCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCHHCCC
QRSASTSNRNFEGRQGKNGLTHLVSPAMAAAASIAGHFVDIRDWA
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA