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The map label for this gene is leuD

Identifier: 119945382

GI number: 119945382

Start: 2029246

End: 2029860

Strand: Direct

Name: leuD

Synonym: Ping_1670

Alternate gene names: 119945382

Gene position: 2029246-2029860 (Clockwise)

Preceding gene: 119945381

Following gene: 119945386

Centisome position: 44.5

GC content: 37.89

Gene sequence:

>615_bases
ATGGAAGCTTATAAAAAACATACTAGCATTGCGGCATTAATGAATCGCAGTAACGTTGATACAGACCAAATTATTCCAAC
GCAGTTCTTGAAAAAAGTAGAGCGTACGGGTTTTGGTATCCACCTTTTTCACGATTGGCGTTTTTTGGCCGATAATGTCA
CTCCTAACCCTGAATTTGAGCTTAATAAACCGGTCTTTAAAGGTGCTAAAATCTTAGTTACCGGTGATAACTTCGGTTGT
GGCTCTTCGCGCGAACATGCTCCTTGGGCAATTGCTGATTACGGTTTTAATACTGTTATATCAACCAGTTTTGCTGATAT
TTTTTATACTAACTGTTTTAAAAATGCGCTTCTGCCCATTAGAGTAAGCAAAGATGAACTTGCTGCATTAATGGCTGAGA
TCTCGGCTAATGAAGGGGTTAAATTTACGGTTGATCTTGAAGCTGAAAAATTAACCACACCAGGCGGCATTGTTATTCAT
ATTGAAGTGGATCCGTTTCGTAAAGAATCTTTATTGGGCGGTCTTGACGATATTGCTTGGACGTTAAAACACGAAGATAA
AATTACTGCTTTTGAAGAAAAACAAAAACAAACTTTACCATGGTTATGGAAATAG

Upstream 100 bases:

>100_bases
AAAATGGACTTACTCACCTTGTTTCGCCTGCAATGGCGGCAGCGGCATCTATTGCCGGACACTTTGTAGATATTCGAGAT
TGGGCATAAAGGGAACAATA

Downstream 100 bases:

>100_bases
TCGTTTTTAACTTCTAAAAAATGAGATTGATTCAAAGGCGCATTTATTGCGCCTTTTTTATTTTGGCATTGGGTAGATAA
CTTTAATAGAGAAAGGCAAT

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MEAYKKHTSIAALMNRSNVDTDQIIPTQFLKKVERTGFGIHLFHDWRFLADNVTPNPEFELNKPVFKGAKILVTGDNFGC
GSSREHAPWAIADYGFNTVISTSFADIFYTNCFKNALLPIRVSKDELAALMAEISANEGVKFTVDLEAEKLTTPGGIVIH
IEVDPFRKESLLGGLDDIAWTLKHEDKITAFEEKQKQTLPWLWK

Sequences:

>Translated_204_residues
MEAYKKHTSIAALMNRSNVDTDQIIPTQFLKKVERTGFGIHLFHDWRFLADNVTPNPEFELNKPVFKGAKILVTGDNFGC
GSSREHAPWAIADYGFNTVISTSFADIFYTNCFKNALLPIRVSKDELAALMAEISANEGVKFTVDLEAEKLTTPGGIVIH
IEVDPFRKESLLGGLDDIAWTLKHEDKITAFEEKQKQTLPWLWK
>Mature_204_residues
MEAYKKHTSIAALMNRSNVDTDQIIPTQFLKKVERTGFGIHLFHDWRFLADNVTPNPEFELNKPVFKGAKILVTGDNFGC
GSSREHAPWAIADYGFNTVISTSFADIFYTNCFKNALLPIRVSKDELAALMAEISANEGVKFTVDLEAEKLTTPGGIVIH
IEVDPFRKESLLGGLDDIAWTLKHEDKITAFEEKQKQTLPWLWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=53.3333333333333, Blast_Score=218, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=207, Percent_Identity=45.8937198067633, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_PSYIN (A1SVE8)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943062.1
- STRING:   A1SVE8
- GeneID:   4626919
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1670
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   A1SVE8
- BioCyc:   PING357804:PING_1670-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23016; Mature: 23016

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAYKKHTSIAALMNRSNVDTDQIIPTQFLKKVERTGFGIHLFHDWRFLADNVTPNPEFE
CCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCE
LNKPVFKGAKILVTGDNFGCGSSREHAPWAIADYGFNTVISTSFADIFYTNCFKNALLPI
ECCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEE
RVSKDELAALMAEISANEGVKFTVDLEAEKLTTPGGIVIHIEVDPFRKESLLGGLDDIAW
EECHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHEE
TLKHEDKITAFEEKQKQTLPWLWK
EEECCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEAYKKHTSIAALMNRSNVDTDQIIPTQFLKKVERTGFGIHLFHDWRFLADNVTPNPEFE
CCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCE
LNKPVFKGAKILVTGDNFGCGSSREHAPWAIADYGFNTVISTSFADIFYTNCFKNALLPI
ECCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEE
RVSKDELAALMAEISANEGVKFTVDLEAEKLTTPGGIVIHIEVDPFRKESLLGGLDDIAW
EECHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHEE
TLKHEDKITAFEEKQKQTLPWLWK
EEECCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA