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The map label for this gene is prpD [H]

Identifier: 119945290

GI number: 119945290

Start: 1920976

End: 1922421

Strand: Direct

Name: prpD [H]

Synonym: Ping_1572

Alternate gene names: 119945290

Gene position: 1920976-1922421 (Clockwise)

Preceding gene: 119945285

Following gene: 119945291

Centisome position: 42.13

GC content: 43.91

Gene sequence:

>1446_bases
ATGAGTACTAATGTTGAATCAAATATTAAACCGGACTATGACCAACCCATCAAAGATATAACCAATTATGTGACCGATTA
TGCGATAAAAAATGACCATTTGTATGATACCGCCCGTTTTTGTTTAATGGATACTCTGGGCTGTGGTCTTTTAGCACTTA
GATACCCGGAATGCAGCAAACATTTAGGACCCATAGTTCGTGGAACCATAGTGCCCAACGGTGCGCGAGTCCCGGGTACT
CAATTTGAAATGGACCCCATTAAAGCTGCGTTTGATATTGGTTGTATTATCCGGTGGCTAGATTTTAATGATACCTGGTT
AGCCGCTGAGTGGGGCCACCCCTCGGATAACATGGGCGGAATTTTAGCGGTTGCTGATTATGTTTCACGTCAAAGCCTGG
CCAGCGGTTATGAACCACTGACTATGAAAGATGTGCTCACGGCCATGGTCAAAGCACATGAGATCCAGGGAGTGCTAGCG
TTAGAGAATAGTTTTAACCGGGTAGGGCTGGACCATGTGATTTTAGTCAAGATAGCGACCACAGCGGTGGTGACTCAAAT
GTTGGGCGGGGGATCTGAAGAAATAATGGATGCACTTTCCCAGGCACTGGTCGATGGGCAAAGTTTGCGAACTTATCGGC
ACTCGCCAAATGCCGGTTCACGTAAATCCTGGGCAGCTGGTGATGCGACCAGTCGGGCAGTGCGTTTAGCGATGATCACT
TTGTCGGGCGAAATGGGTTATCCGGGCGTATTAAGCGCCAAAAACTGGGGGTTTTATGACGTGTTGTTTGGCGGTAAAGA
ATTTAAATTGCAGCAACCTTATGGTGAATATGTCATGGATAATGTGTTATTTAAAATTTCTTATCCCGCAGAATTCCATG
CTCAGACTGCGGTTGAATGTGCGGCTAACTTGCACCACAAGGTGGTCAATTGTTTAACTGAAATAGATCGCGTTGAATTA
ACAACACACCAATCAGCCATTCGAATTATCAGCAAACAAGGTGCGCTGCATAATCCGGCCGATCGCGATCACTGTTTGCA
ATACATGGTCGCCATAGGATTAATTTTTGGTGATTTAAATGCGGACCACTATGAAGATGAAGTAGCAGCTGATGGTCGTA
TTGATACGTTAAGATCAAAAATGCATGTCATTGAAGATAAGTCTTACAGTGAGGATTATCTCGACCCTGAAAAACGCAGC
ATAGCTAACGCAATTCAAATTTTCTTTAAAGATGGCAGCAGCACAGAAAAAGTGGTAATGGAATATCCGATTGGTCATAA
ACTTCGCAGAGAAGAAGGGATCCCGTTGCTACAAGCCAAATTTGAACGTAATGTTAAAACACGATTCCCTGATAAACATG
CAAGGACAATAGTGGAAATCTGCAGTGACAAGCAAGAGTTCGAAGCAATGCCGGTAAATGAATTTATGGATTTGCTGGTT
ATTTGA

Upstream 100 bases:

>100_bases
GAACGCCTACTTTTGATACTGTACCTTATCTGGTCGTCGACCGCGGTCGAGATGACAGTCTCATTTTTATTAGAATATCG
ATAATTATCTGGAGTTATGC

Downstream 100 bases:

>100_bases
GATGAAGAGTTTTTACCGATAGTCTGTTTAGGTTGTTTGTAAAATATTTTGTTTTATTTCTATTAAATCAAGGTCAAAAG
CGTTAGCGCTGTGAAACGTC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSTNVESNIKPDYDQPIKDITNYVTDYAIKNDHLYDTARFCLMDTLGCGLLALRYPECSKHLGPIVRGTIVPNGARVPGT
QFEMDPIKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNMGGILAVADYVSRQSLASGYEPLTMKDVLTAMVKAHEIQGVLA
LENSFNRVGLDHVILVKIATTAVVTQMLGGGSEEIMDALSQALVDGQSLRTYRHSPNAGSRKSWAAGDATSRAVRLAMIT
LSGEMGYPGVLSAKNWGFYDVLFGGKEFKLQQPYGEYVMDNVLFKISYPAEFHAQTAVECAANLHHKVVNCLTEIDRVEL
TTHQSAIRIISKQGALHNPADRDHCLQYMVAIGLIFGDLNADHYEDEVAADGRIDTLRSKMHVIEDKSYSEDYLDPEKRS
IANAIQIFFKDGSSTEKVVMEYPIGHKLRREEGIPLLQAKFERNVKTRFPDKHARTIVEICSDKQEFEAMPVNEFMDLLV
I

Sequences:

>Translated_481_residues
MSTNVESNIKPDYDQPIKDITNYVTDYAIKNDHLYDTARFCLMDTLGCGLLALRYPECSKHLGPIVRGTIVPNGARVPGT
QFEMDPIKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNMGGILAVADYVSRQSLASGYEPLTMKDVLTAMVKAHEIQGVLA
LENSFNRVGLDHVILVKIATTAVVTQMLGGGSEEIMDALSQALVDGQSLRTYRHSPNAGSRKSWAAGDATSRAVRLAMIT
LSGEMGYPGVLSAKNWGFYDVLFGGKEFKLQQPYGEYVMDNVLFKISYPAEFHAQTAVECAANLHHKVVNCLTEIDRVEL
TTHQSAIRIISKQGALHNPADRDHCLQYMVAIGLIFGDLNADHYEDEVAADGRIDTLRSKMHVIEDKSYSEDYLDPEKRS
IANAIQIFFKDGSSTEKVVMEYPIGHKLRREEGIPLLQAKFERNVKTRFPDKHARTIVEICSDKQEFEAMPVNEFMDLLV
I
>Mature_480_residues
STNVESNIKPDYDQPIKDITNYVTDYAIKNDHLYDTARFCLMDTLGCGLLALRYPECSKHLGPIVRGTIVPNGARVPGTQ
FEMDPIKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNMGGILAVADYVSRQSLASGYEPLTMKDVLTAMVKAHEIQGVLAL
ENSFNRVGLDHVILVKIATTAVVTQMLGGGSEEIMDALSQALVDGQSLRTYRHSPNAGSRKSWAAGDATSRAVRLAMITL
SGEMGYPGVLSAKNWGFYDVLFGGKEFKLQQPYGEYVMDNVLFKISYPAEFHAQTAVECAANLHHKVVNCLTEIDRVELT
THQSAIRIISKQGALHNPADRDHCLQYMVAIGLIFGDLNADHYEDEVAADGRIDTLRSKMHVIEDKSYSEDYLDPEKRSI
ANAIQIFFKDGSSTEKVVMEYPIGHKLRREEGIPLLQAKFERNVKTRFPDKHARTIVEICSDKQEFEAMPVNEFMDLLVI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=484, Percent_Identity=62.8099173553719, Blast_Score=654, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=490, Percent_Identity=54.4897959183673, Blast_Score=550, Evalue=1e-157,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53659; Mature: 53528

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNVESNIKPDYDQPIKDITNYVTDYAIKNDHLYDTARFCLMDTLGCGLLALRYPECSK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEECCHHHH
HLGPIVRGTIVPNGARVPGTQFEMDPIKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNMGG
HCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCC
ILAVADYVSRQSLASGYEPLTMKDVLTAMVKAHEIQGVLALENSFNRVGLDHVILVKIAT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEHHHHH
TAVVTQMLGGGSEEIMDALSQALVDGQSLRTYRHSPNAGSRKSWAAGDATSRAVRLAMIT
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEEEE
LSGEMGYPGVLSAKNWGFYDVLFGGKEFKLQQPYGEYVMDNVLFKISYPAEFHAQTAVEC
EECCCCCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHCEEEEEECCHHHHHHHHHHH
AANLHHKVVNCLTEIDRVELTTHQSAIRIISKQGALHNPADRDHCLQYMVAIGLIFGDLN
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ADHYEDEVAADGRIDTLRSKMHVIEDKSYSEDYLDPEKRSIANAIQIFFKDGSSTEKVVM
CCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEE
EYPIGHKLRREEGIPLLQAKFERNVKTRFPDKHARTIVEICSDKQEFEAMPVNEFMDLLV
ECCCCCHHHHHCCCCHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHC
I
C
>Mature Secondary Structure 
STNVESNIKPDYDQPIKDITNYVTDYAIKNDHLYDTARFCLMDTLGCGLLALRYPECSK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEECCHHHH
HLGPIVRGTIVPNGARVPGTQFEMDPIKAAFDIGCIIRWLDFNDTWLAAEWGHPSDNMGG
HCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCC
ILAVADYVSRQSLASGYEPLTMKDVLTAMVKAHEIQGVLALENSFNRVGLDHVILVKIAT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEHHHHH
TAVVTQMLGGGSEEIMDALSQALVDGQSLRTYRHSPNAGSRKSWAAGDATSRAVRLAMIT
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEEEE
LSGEMGYPGVLSAKNWGFYDVLFGGKEFKLQQPYGEYVMDNVLFKISYPAEFHAQTAVEC
EECCCCCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHCEEEEEECCHHHHHHHHHHH
AANLHHKVVNCLTEIDRVELTTHQSAIRIISKQGALHNPADRDHCLQYMVAIGLIFGDLN
HHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
ADHYEDEVAADGRIDTLRSKMHVIEDKSYSEDYLDPEKRSIANAIQIFFKDGSSTEKVVM
CCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEE
EYPIGHKLRREEGIPLLQAKFERNVKTRFPDKHARTIVEICSDKQEFEAMPVNEFMDLLV
ECCCCCHHHHHCCCCHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]