Click here to switch to the map view.

The map label for this gene is nusB

Identifier: 119945180

GI number: 119945180

Start: 1777320

End: 1777757

Strand: Direct

Name: nusB

Synonym: Ping_1444

Alternate gene names: 119945180

Gene position: 1777320-1777757 (Clockwise)

Preceding gene: 119945179

Following gene: 119945181

Centisome position: 38.98

GC content: 37.21

Gene sequence:

>438_bases
ATGAAAATGAAACCAGCTGAACGTCGACGCGCCCGCCAATCTGCGGTACAGGCAATTTACCAATGGCAAATCACTAAACT
GAGTGCCGGTCAAATTATTGAGCAATTTAGCACTGATCAGGATCTGTCAAAAACAGATGTTCCGTATTTTAAAGAATTAT
TAACCGGTGTTATTCATCGTGTTGATTTTTTAGATGAAAAACTATCTCCTTATCTTTCCCGTAAAATTGAAGATGTTGAT
ATGGTTGATAAAGCCGTATTACGTTTAGCCATGTTTGAGTTAACTCAGCGTACCGATATTCCACATAAAGTGGTTTTGAA
TGAAGCGATTGAATTAGCAAAAGATTTTGCTACGGATGAAAGCTACAAGTTCGTTAATGGTGTTTTAGATAAAGCATTAC
GCAGTTTAAAACTACGAGAAGAAGAACAGGGCAAGTAA

Upstream 100 bases:

>100_bases
AACAAGCGATTGAACGTGCGGGAACTAAAATGGGTAATAAAGGCAGTGAAGCGGCCTTAAGCGCATTAGAAATGGTCAAT
GTTATGGCGGAGTTTAAATA

Downstream 100 bases:

>100_bases
TACTGCTTCTAAGGTTAGGTTTATATCCCAACTACTTGATGAAGATGCAGGCTTCTGCATCTTCATCAAGTAGTTGGGAA
TAGAATAAAAAATCCAGTAG

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKMKPAERRRARQSAVQAIYQWQITKLSAGQIIEQFSTDQDLSKTDVPYFKELLTGVIHRVDFLDEKLSPYLSRKIEDVD
MVDKAVLRLAMFELTQRTDIPHKVVLNEAIELAKDFATDESYKFVNGVLDKALRSLKLREEEQGK

Sequences:

>Translated_145_residues
MKMKPAERRRARQSAVQAIYQWQITKLSAGQIIEQFSTDQDLSKTDVPYFKELLTGVIHRVDFLDEKLSPYLSRKIEDVD
MVDKAVLRLAMFELTQRTDIPHKVVLNEAIELAKDFATDESYKFVNGVLDKALRSLKLREEEQGK
>Mature_145_residues
MKMKPAERRRARQSAVQAIYQWQITKLSAGQIIEQFSTDQDLSKTDVPYFKELLTGVIHRVDFLDEKLSPYLSRKIEDVD
MVDKAVLRLAMFELTQRTDIPHKVVLNEAIELAKDFATDESYKFVNGVLDKALRSLKLREEEQGK

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=130, Percent_Identity=54.6153846153846, Blast_Score=140, Evalue=3e-35,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_PSYIN (A1SUU6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942860.1
- ProteinModelPortal:   A1SUU6
- SMR:   A1SUU6
- STRING:   A1SUU6
- GeneID:   4624561
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1444
- eggNOG:   COG0781
- HOGENOM:   HBG734409
- OMA:   IPYKVVI
- PhylomeDB:   A1SUU6
- BioCyc:   PING357804:PING_1444-MONOMER
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 16817; Mature: 16817

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKPAERRRARQSAVQAIYQWQITKLSAGQIIEQFSTDQDLSKTDVPYFKELLTGVIHR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VDFLDEKLSPYLSRKIEDVDMVDKAVLRLAMFELTQRTDIPHKVVLNEAIELAKDFATDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
SYKFVNGVLDKALRSLKLREEEQGK
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKMKPAERRRARQSAVQAIYQWQITKLSAGQIIEQFSTDQDLSKTDVPYFKELLTGVIHR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VDFLDEKLSPYLSRKIEDVDMVDKAVLRLAMFELTQRTDIPHKVVLNEAIELAKDFATDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
SYKFVNGVLDKALRSLKLREEEQGK
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA