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The map label for this gene is holA [H]

Identifier: 119944942

GI number: 119944942

Start: 1479764

End: 1480759

Strand: Reverse

Name: holA [H]

Synonym: Ping_1189

Alternate gene names: 119944942

Gene position: 1480759-1479764 (Counterclockwise)

Preceding gene: 119944943

Following gene: 119944941

Centisome position: 32.48

GC content: 35.84

Gene sequence:

>996_bases
ATGCGTATTTACCCTGAACAATTGAACCAACACCTTACGCAAAAAATACCCAGCTGCTGTTTGATTTTCGGTGATGAGGC
GCTACTTTGTTTAGAAGCGACAGAGCAAATTCAGAAAGCGGCAAAAAAACAAGGATATTTGGAAAAATTAAGTTTTAGTC
TTGATGGTAACTTTGACAATGATCTTATTTTTGGCGAGTTTCAAACGCTCTCCCTTTTTTCTGAAAAAAAGATTATATTA
TTAACCCTGACTAAAACAAACAAAGAAAACACTGCTTTTATTCGAGAAATTACGCCCCTATTACATCCAGATATCCTGTT
AATTTTACAGGGGCCAAAACTCAACAATCAACAAATGAGCAGTGTTTGGTTTAAAACACTAGAACAAAAGGGAATCTTTG
TTGCGACCAACCTACCACAAGCGCATCGTTTTCCCCAGTGGGTTTTTCAGCGTCTTAAAGCACTCAATTTGCACGCAGAT
AAAGATATTATTGATTATCTTTGTTTGCATTTTGAAGGTAATTTATTGGCAGCTAAACAAGAATTTGAAAAATTGGCGCT
GTTATATCCGAAGCAAAATTTAACCCTGCAGCAAGTTGAGCAAAGCATTACTACCCACAACCATTTTAATCTTTTCCAAT
GGATTGATAGTTTGTTGGCCGGCGATCAGATGCGTTCCGTGCGCATTTTAAAACAGTTAAAAGCCGAAGGAACTGAGCTT
TTATTACTCTGTGCAACATTGAATAATGAAGTCCAAAAGCTCCTTAAACTTAGCTATCAAAGCCAACAGCATTCTTTAAC
GCAATTATTCAACCAGCAGCAACCAAAACTTTGGCCGGCAAAACAACAGTTAATAAGAGATGCATTAACACGTTTGAATA
TCAACAAGCTTGAAAAAATGATTATTCAGTGTGCAGAAATAGAAATTTCTATTAAAGTAGAAAACAAAGATTCGAATTGG
TTAAAACTTAACGAGATTACCTACTCTTTTTTCTGA

Upstream 100 bases:

>100_bases
CGCGAAGCGGAAATATTAACCAAAGAATTGCGCGGTATTGCTGCTGACAGTATTATTGCCACTATGCTCAGCCTAGAAAA
TAAAAAATAAGCGATAAAAA

Downstream 100 bases:

>100_bases
TTTCAAAGGACAGTTATCAATATGGATATCCAACAGCAGGCCATCGGTTTTTTAGGGGGTACTTTTGATCCCATTCATTT
TGGGCATTTGCGTCCGGCAC

Product: DNA polymerase III, delta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRIYPEQLNQHLTQKIPSCCLIFGDEALLCLEATEQIQKAAKKQGYLEKLSFSLDGNFDNDLIFGEFQTLSLFSEKKIIL
LTLTKTNKENTAFIREITPLLHPDILLILQGPKLNNQQMSSVWFKTLEQKGIFVATNLPQAHRFPQWVFQRLKALNLHAD
KDIIDYLCLHFEGNLLAAKQEFEKLALLYPKQNLTLQQVEQSITTHNHFNLFQWIDSLLAGDQMRSVRILKQLKAEGTEL
LLLCATLNNEVQKLLKLSYQSQQHSLTQLFNQQQPKLWPAKQQLIRDALTRLNINKLEKMIIQCAEIEISIKVENKDSNW
LKLNEITYSFF

Sequences:

>Translated_331_residues
MRIYPEQLNQHLTQKIPSCCLIFGDEALLCLEATEQIQKAAKKQGYLEKLSFSLDGNFDNDLIFGEFQTLSLFSEKKIIL
LTLTKTNKENTAFIREITPLLHPDILLILQGPKLNNQQMSSVWFKTLEQKGIFVATNLPQAHRFPQWVFQRLKALNLHAD
KDIIDYLCLHFEGNLLAAKQEFEKLALLYPKQNLTLQQVEQSITTHNHFNLFQWIDSLLAGDQMRSVRILKQLKAEGTEL
LLLCATLNNEVQKLLKLSYQSQQHSLTQLFNQQQPKLWPAKQQLIRDALTRLNINKLEKMIIQCAEIEISIKVENKDSNW
LKLNEITYSFF
>Mature_331_residues
MRIYPEQLNQHLTQKIPSCCLIFGDEALLCLEATEQIQKAAKKQGYLEKLSFSLDGNFDNDLIFGEFQTLSLFSEKKIIL
LTLTKTNKENTAFIREITPLLHPDILLILQGPKLNNQQMSSVWFKTLEQKGIFVATNLPQAHRFPQWVFQRLKALNLHAD
KDIIDYLCLHFEGNLLAAKQEFEKLALLYPKQNLTLQQVEQSITTHNHFNLFQWIDSLLAGDQMRSVRILKQLKAEGTEL
LLLCATLNNEVQKLLKLSYQSQQHSLTQLFNQQQPKLWPAKQQLIRDALTRLNINKLEKMIIQCAEIEISIKVENKDSNW
LKLNEITYSFF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=330, Percent_Identity=35.4545454545455, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38453; Mature: 38453

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYPEQLNQHLTQKIPSCCLIFGDEALLCLEATEQIQKAAKKQGYLEKLSFSLDGNFDN
CCCCHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHEEEECCCCCC
DLIFGEFQTLSLFSEKKIILLTLTKTNKENTAFIREITPLLHPDILLILQGPKLNNQQMS
CEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
SVWFKTLEQKGIFVATNLPQAHRFPQWVFQRLKALNLHADKDIIDYLCLHFEGNLLAAKQ
HHHHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHH
EFEKLALLYPKQNLTLQQVEQSITTHNHFNLFQWIDSLLAGDQMRSVRILKQLKAEGTEL
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
LLLCATLNNEVQKLLKLSYQSQQHSLTQLFNQQQPKLWPAKQQLIRDALTRLNINKLEKM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
IIQCAEIEISIKVENKDSNWLKLNEITYSFF
HHHHHEEEEEEEEECCCCCEEEEECEEEECC
>Mature Secondary Structure
MRIYPEQLNQHLTQKIPSCCLIFGDEALLCLEATEQIQKAAKKQGYLEKLSFSLDGNFDN
CCCCHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHEEEECCCCCC
DLIFGEFQTLSLFSEKKIILLTLTKTNKENTAFIREITPLLHPDILLILQGPKLNNQQMS
CEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
SVWFKTLEQKGIFVATNLPQAHRFPQWVFQRLKALNLHADKDIIDYLCLHFEGNLLAAKQ
HHHHHHHHHCCEEEEECCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHH
EFEKLALLYPKQNLTLQQVEQSITTHNHFNLFQWIDSLLAGDQMRSVRILKQLKAEGTEL
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
LLLCATLNNEVQKLLKLSYQSQQHSLTQLFNQQQPKLWPAKQQLIRDALTRLNINKLEKM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
IIQCAEIEISIKVENKDSNWLKLNEITYSFF
HHHHHEEEEEEEEECCCCCEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]