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The map label for this gene is nrdB [H]

Identifier: 119944866

GI number: 119944866

Start: 1385206

End: 1386351

Strand: Reverse

Name: nrdB [H]

Synonym: Ping_1109

Alternate gene names: 119944866

Gene position: 1386351-1385206 (Counterclockwise)

Preceding gene: 119944869

Following gene: 119944865

Centisome position: 30.41

GC content: 38.22

Gene sequence:

>1146_bases
ATGGCTTATACAACTTTCTCGACAATTCCTAATGATGCAATGAAGGAACCCATGTTCTTTGGCAATCCGGTCAATGTAGC
GCGTTATGACCAACAACGCTTTGCTGTATTTGAAATGTTAATTGAAAAGCAGTTATCGTTTTTCTGGCGACCAGAGGAGA
TTGATGTAAGCCGTGACCGTATCGATTTTTTAAACTTACCAGATCATGAGCAGCATATTTTCATCTCCAATCTAAAATAC
CAAACATTATTAGATTCAATTCAGGGTCGTAGCCCGAACATTGCGCTACTACCTATTGTTTCATTACCAGAACTTGAAAC
TTGGATTGAGACATGGTCATTTTCTGAAACAATTCACAGTCGCAGTTACACCCATATTTTACGTAATCTATTTACTGAAC
CGGCTGAAATTTTTGAAGATATCGTCACTAACGTAGAAATTCAAAAGCGCGCGACGGATATTTCCGGTTATTATGATGCT
TTAATCCATGCTACGCAGTTTATGCAACTGCACGGATTGGAACAAACTGACATTGATTATATCAATCTGGAAAATCAAGT
TGAAAAATTAAACCTGCGCGCCATTAAGAAAAAACTTTACCTTTGTATCTGCTCTGTTAATGCGTTAGAAGCCATTCGTT
TTTATGTTTCTTTTGCTTGTTCATTTGCCTTTGCCGAACGTGATCTATTAGAAGGCAATGCAAAAATAATAAAATTAATT
GCGCGGGACGAAGCCCTTCACCTCAATTCGACGCAACAAATTTTAAACCTTTGGGCTGATGGAAAAGATGATCCTGAAAT
GGCTGAGATTGCTATTGAATGTTTTGCTGAAGGGCGGGAAATATTTTTACAAGCGGCTCAGCAAGAAAAAGATTGGGCTA
AGTACCTGTTTAAAGACGGATCAATGATTGGCCTAAATGAACAAATATTGTCCCAATATGTTGAATATATTGCAAATCTG
CGTATGCAGGCAATTGGTTATGCCCCTGAGTTTGAAGTTAAGAGTAACCCAATTCCATGGATTAATAATTGGCTGGTAAG
TGATAACGTACAGGTTGCTCCGCAAGAGACAGAAATCAGTTCCTATCTGGTAGGGCAAATTGACAGTAAAGTTGACAGTG
AAGATCTCAGTACCTTTGAGTTATAA

Upstream 100 bases:

>100_bases
GAGCATTCTATTGATTATAATTAAACTTGCTTAGCCTCGCTAACAACAATAAAATTTTAGACTCTTTATTCATAATTTAA
ACGTTCATCTGGAGCATTCT

Downstream 100 bases:

>100_bases
ACAGCCATTATGAGTAAAAAAATAACCGTTAACGGGATTGAATATCCACTCGATACCAAAAAAACATTGCTTGAGAATTT
GGAAGCTCAAGCAATTCATC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAYTTFSTIPNDAMKEPMFFGNPVNVARYDQQRFAVFEMLIEKQLSFFWRPEEIDVSRDRIDFLNLPDHEQHIFISNLKY
QTLLDSIQGRSPNIALLPIVSLPELETWIETWSFSETIHSRSYTHILRNLFTEPAEIFEDIVTNVEIQKRATDISGYYDA
LIHATQFMQLHGLEQTDIDYINLENQVEKLNLRAIKKKLYLCICSVNALEAIRFYVSFACSFAFAERDLLEGNAKIIKLI
ARDEALHLNSTQQILNLWADGKDDPEMAEIAIECFAEGREIFLQAAQQEKDWAKYLFKDGSMIGLNEQILSQYVEYIANL
RMQAIGYAPEFEVKSNPIPWINNWLVSDNVQVAPQETEISSYLVGQIDSKVDSEDLSTFEL

Sequences:

>Translated_381_residues
MAYTTFSTIPNDAMKEPMFFGNPVNVARYDQQRFAVFEMLIEKQLSFFWRPEEIDVSRDRIDFLNLPDHEQHIFISNLKY
QTLLDSIQGRSPNIALLPIVSLPELETWIETWSFSETIHSRSYTHILRNLFTEPAEIFEDIVTNVEIQKRATDISGYYDA
LIHATQFMQLHGLEQTDIDYINLENQVEKLNLRAIKKKLYLCICSVNALEAIRFYVSFACSFAFAERDLLEGNAKIIKLI
ARDEALHLNSTQQILNLWADGKDDPEMAEIAIECFAEGREIFLQAAQQEKDWAKYLFKDGSMIGLNEQILSQYVEYIANL
RMQAIGYAPEFEVKSNPIPWINNWLVSDNVQVAPQETEISSYLVGQIDSKVDSEDLSTFEL
>Mature_380_residues
AYTTFSTIPNDAMKEPMFFGNPVNVARYDQQRFAVFEMLIEKQLSFFWRPEEIDVSRDRIDFLNLPDHEQHIFISNLKYQ
TLLDSIQGRSPNIALLPIVSLPELETWIETWSFSETIHSRSYTHILRNLFTEPAEIFEDIVTNVEIQKRATDISGYYDAL
IHATQFMQLHGLEQTDIDYINLENQVEKLNLRAIKKKLYLCICSVNALEAIRFYVSFACSFAFAERDLLEGNAKIIKLIA
RDEALHLNSTQQILNLWADGKDDPEMAEIAIECFAEGREIFLQAAQQEKDWAKYLFKDGSMIGLNEQILSQYVEYIANLR
MQAIGYAPEFEVKSNPIPWINNWLVSDNVQVAPQETEISSYLVGQIDSKVDSEDLSTFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=381, Percent_Identity=71.6535433070866, Blast_Score=574, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 44056; Mature: 43925

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTTFSTIPNDAMKEPMFFGNPVNVARYDQQRFAVFEMLIEKQLSFFWRPEEIDVSRDR
CCEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDFLNLPDHEQHIFISNLKYQTLLDSIQGRSPNIALLPIVSLPELETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHILRNLFTEPAEIFEDIVTNVEIQKRATDISGYYDALIHATQFMQLHGLEQTDIDY
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
INLENQVEKLNLRAIKKKLYLCICSVNALEAIRFYVSFACSFAFAERDLLEGNAKIIKLI
EECHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEE
ARDEALHLNSTQQILNLWADGKDDPEMAEIAIECFAEGREIFLQAAQQEKDWAKYLFKDG
ECCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SMIGLNEQILSQYVEYIANLRMQAIGYAPEFEVKSNPIPWINNWLVSDNVQVAPQETEIS
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCEEECCCCEECCCHHHHH
SYLVGQIDSKVDSEDLSTFEL
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AYTTFSTIPNDAMKEPMFFGNPVNVARYDQQRFAVFEMLIEKQLSFFWRPEEIDVSRDR
CEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDFLNLPDHEQHIFISNLKYQTLLDSIQGRSPNIALLPIVSLPELETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHILRNLFTEPAEIFEDIVTNVEIQKRATDISGYYDALIHATQFMQLHGLEQTDIDY
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
INLENQVEKLNLRAIKKKLYLCICSVNALEAIRFYVSFACSFAFAERDLLEGNAKIIKLI
EECHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEE
ARDEALHLNSTQQILNLWADGKDDPEMAEIAIECFAEGREIFLQAAQQEKDWAKYLFKDG
ECCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SMIGLNEQILSQYVEYIANLRMQAIGYAPEFEVKSNPIPWINNWLVSDNVQVAPQETEIS
CEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCEEECCCCEECCCHHHHH
SYLVGQIDSKVDSEDLSTFEL
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]