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The map label for this gene is nrdA [H]

Identifier: 119944869

GI number: 119944869

Start: 1391267

End: 1393540

Strand: Reverse

Name: nrdA [H]

Synonym: Ping_1112

Alternate gene names: 119944869

Gene position: 1393540-1391267 (Counterclockwise)

Preceding gene: 119944870

Following gene: 119944866

Centisome position: 30.56

GC content: 40.24

Gene sequence:

>2274_bases
ATGAAAAAAAACCTATTTGTCACAAAACGAAATGGCAAAAGAGAACGCATTGATCTTGATAAGATTCACCGAGTTATCAC
TTGGGCTGCAGAAGGCTTAAAAAATGTTTCAGTCTCTCAGGTTGAATTAAATTCACATATTCAGTTTTATGATGGTATCC
GTACCGATGATATACATGAGACTATTATTAAAGCGGCCGCTGACCTTATCTCAGAGACATCACCAGACTACCAATATTTA
GCGGCACATCTTGCGGTATTCCATTTGCGTAAAAAAGCTTATCGACGTTTTACGCCGCCAAGTATCTTTGAACACGTTAG
TAAAATGTGTTCTAAAGGCAAGTACGATATGCATCTTCTTGAAGACTATAGCAAGCAAGAGTTTGAAGAGATGGAAAAAT
TTATCGTTCATGATCGTGATTTAAAATTTTCCTATGCCGCAGTAAAACAGCTTGAAGGTAAATACCTCATTCAAAATCGT
GTCACCGGTGATATTTATGAAAGTGCACAATTTCTTTACATGATGGTGGCAGCCTGTCTGTTTTCTAATTATGAGCCTGC
CATACGTTTATCTTATGTTAGACGTTTTTATGATGCGGTTTCACAATTTAAAATTTCATTACCCACGCCTATTATGGCAG
GAGTGCGCACGCCAACGCGTCAATTTAGTTCATGCGTATTGATTGAAACGGGTGATAGTCTTGATTCGATCAACGCGACA
TCAAGCGCAATTGTTAAATATGTTTCACAACGCGCCGGAATCGGCATTAATGCTGGTCGTATTCGTGCATTGGGCAGTGC
TATTCGAGGTGGTGAGGCCTTCCACACAGGTTGTATCCCATTTTACAAACACTTCCAAACCGCGGTAAAGTCATGCTCTC
AGGGTGGCGTACGCGGCGGTGCAGCGACGGTTTTTTACCCTGTTTGGCATTTGGAAGTTGAATCTTTATTGGTGTTAAAA
AATAATCGTGGTATTGACTCCAACCGCATACGTCATCTCGACTATGGGGTGCAACTAAGCCGCTTATTCTACCAGCGCCT
GATAGACAAAAAAAATGTCGCCTTGTTCAGTCCCTCGGATGTCCCCGGTTTATACGATGCTTTTTTTGAAGATCAAGATG
AATTTGAGCGTTTATATAATTTATACGAAGGTGATCCTGATATTCGTCAAAAAAGTATTTCAGCCGTTGAGTTGTTCTCT
CTCTTAATGCAGGAGCGTGCCAGTACAGGCCGTATTTACATTCAAAATGTAGATCACTGTAATACACACAGTCCCTTTGA
CAGTAAGGTTGCCCCTATTCGTCAAAGTAACTTATGTCTTGAAATTGCCCTGCCAACGAAACCCTTGCAGCACATCATGG
ATGAAGAAGGTGAAATTGCCCTGTGTACCTTATCGGCATTTAACTTAGGCGCAATTGAGTCACTTGATGAACTGGAAGAG
CTTGCAGAACTTGCTGTGCGCGCATTAGATAGCTTATTGGATTATCAGGATTACCCGGTCAAAGCAGCTGAAAACAGCAG
TATGGCACGTCGAACGCTAGGCATAGGGGTTATCAACTTCGCTTATTATTTAGCTAAAAACGGTGTTAAATACTCAGATG
GCAGCGCCAATAATCTAACCCATAAAACCTTTGAAGCCATTCAATATTATCTTTTAAAAGCGTCTAACAAACTAGCTTTT
GAGCGTGGTGCATGCCCTAAATTTAACGAAACACGTTACTCACAGGGTATTTTACCGCTCGATACATACAAAAAGTCCTT
AGATAATATTTGTGATGAGCAGTATCATCTCGATTGGGAAACCCTGCGCAGTGATATTGTTAAACACGGTTTGCGTAATT
CTACCTTATCGGCATTAATGCCCTCTGAAACATCGAGTCAAATATCTAATGCAACTAATGGTATTGAACCACCACGCGGT
TTTGTAAGCGTTAAAGCAAGTAAAGATGGTATTTTAAAGCAAGTGGTGCCTGAGTATGAAAAATATAAACAAAACTATGA
GTTATTGTGGGATATTCCAAACAATCAAGGTTACCTTGAATTAGTTGGCATAATGCAAAAATTCATCGATCAGGCAATTT
CAGCAAATACCAATTACGATCCAAAACGCTTTTCTGAAGGGCGAGTGCCAATGACTAATCTTTTAAAAGACTTGTTGGTT
GCTTATAAAATGGGTGTTAAAACATTGTATTACCACAATACCCGCGATGGTGCAGATGATGAAAACATCATAGATGAAGC
CGATGACTGTGCTGGCGGTGCATGTAAAATATGA

Upstream 100 bases:

>100_bases
GCGCTATCAAACACAATATGTTGTGTTTTTGACCAATGAGCAACACTATTAAAACTGCATGTTAAGTAACACAGTTGTCG
CTAGATATAAGGTATCCTGA

Downstream 100 bases:

>100_bases
CTGGTGAGTTTTTATTAATGTCTCATTTTTGTGGATAAGTTGAAGTAATACTTATATTTAAATAACTTAAAGGATGTGTT
TTACACATCCTTTCTTTTAG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MKKNLFVTKRNGKRERIDLDKIHRVITWAAEGLKNVSVSQVELNSHIQFYDGIRTDDIHETIIKAAADLISETSPDYQYL
AAHLAVFHLRKKAYRRFTPPSIFEHVSKMCSKGKYDMHLLEDYSKQEFEEMEKFIVHDRDLKFSYAAVKQLEGKYLIQNR
VTGDIYESAQFLYMMVAACLFSNYEPAIRLSYVRRFYDAVSQFKISLPTPIMAGVRTPTRQFSSCVLIETGDSLDSINAT
SSAIVKYVSQRAGIGINAGRIRALGSAIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATVFYPVWHLEVESLLVLK
NNRGIDSNRIRHLDYGVQLSRLFYQRLIDKKNVALFSPSDVPGLYDAFFEDQDEFERLYNLYEGDPDIRQKSISAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLQHIMDEEGEIALCTLSAFNLGAIESLDELEE
LAELAVRALDSLLDYQDYPVKAAENSSMARRTLGIGVINFAYYLAKNGVKYSDGSANNLTHKTFEAIQYYLLKASNKLAF
ERGACPKFNETRYSQGILPLDTYKKSLDNICDEQYHLDWETLRSDIVKHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSVKASKDGILKQVVPEYEKYKQNYELLWDIPNNQGYLELVGIMQKFIDQAISANTNYDPKRFSEGRVPMTNLLKDLLV
AYKMGVKTLYYHNTRDGADDENIIDEADDCAGGACKI

Sequences:

>Translated_757_residues
MKKNLFVTKRNGKRERIDLDKIHRVITWAAEGLKNVSVSQVELNSHIQFYDGIRTDDIHETIIKAAADLISETSPDYQYL
AAHLAVFHLRKKAYRRFTPPSIFEHVSKMCSKGKYDMHLLEDYSKQEFEEMEKFIVHDRDLKFSYAAVKQLEGKYLIQNR
VTGDIYESAQFLYMMVAACLFSNYEPAIRLSYVRRFYDAVSQFKISLPTPIMAGVRTPTRQFSSCVLIETGDSLDSINAT
SSAIVKYVSQRAGIGINAGRIRALGSAIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATVFYPVWHLEVESLLVLK
NNRGIDSNRIRHLDYGVQLSRLFYQRLIDKKNVALFSPSDVPGLYDAFFEDQDEFERLYNLYEGDPDIRQKSISAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLQHIMDEEGEIALCTLSAFNLGAIESLDELEE
LAELAVRALDSLLDYQDYPVKAAENSSMARRTLGIGVINFAYYLAKNGVKYSDGSANNLTHKTFEAIQYYLLKASNKLAF
ERGACPKFNETRYSQGILPLDTYKKSLDNICDEQYHLDWETLRSDIVKHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSVKASKDGILKQVVPEYEKYKQNYELLWDIPNNQGYLELVGIMQKFIDQAISANTNYDPKRFSEGRVPMTNLLKDLLV
AYKMGVKTLYYHNTRDGADDENIIDEADDCAGGACKI
>Mature_757_residues
MKKNLFVTKRNGKRERIDLDKIHRVITWAAEGLKNVSVSQVELNSHIQFYDGIRTDDIHETIIKAAADLISETSPDYQYL
AAHLAVFHLRKKAYRRFTPPSIFEHVSKMCSKGKYDMHLLEDYSKQEFEEMEKFIVHDRDLKFSYAAVKQLEGKYLIQNR
VTGDIYESAQFLYMMVAACLFSNYEPAIRLSYVRRFYDAVSQFKISLPTPIMAGVRTPTRQFSSCVLIETGDSLDSINAT
SSAIVKYVSQRAGIGINAGRIRALGSAIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATVFYPVWHLEVESLLVLK
NNRGIDSNRIRHLDYGVQLSRLFYQRLIDKKNVALFSPSDVPGLYDAFFEDQDEFERLYNLYEGDPDIRQKSISAVELFS
LLMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLQHIMDEEGEIALCTLSAFNLGAIESLDELEE
LAELAVRALDSLLDYQDYPVKAAENSSMARRTLGIGVINFAYYLAKNGVKYSDGSANNLTHKTFEAIQYYLLKASNKLAF
ERGACPKFNETRYSQGILPLDTYKKSLDNICDEQYHLDWETLRSDIVKHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSVKASKDGILKQVVPEYEKYKQNYELLWDIPNNQGYLELVGIMQKFIDQAISANTNYDPKRFSEGRVPMTNLLKDLLV
AYKMGVKTLYYHNTRDGADDENIIDEADDCAGGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=791, Percent_Identity=27.9393173198483, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=77.7923784494087, Blast_Score=1256, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=576, Percent_Identity=26.3888888888889, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17555242, Length=646, Percent_Identity=28.9473684210526, Blast_Score=244, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322123, Length=658, Percent_Identity=29.7872340425532, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=29.8165137614679, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17352481, Length=650, Percent_Identity=29.3846153846154, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85676; Mature: 85676

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLFVTKRNGKRERIDLDKIHRVITWAAEGLKNVSVSQVELNSHIQFYDGIRTDDIHE
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHH
TIIKAAADLISETSPDYQYLAAHLAVFHLRKKAYRRFTPPSIFEHVSKMCSKGKYDMHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
EDYSKQEFEEMEKFIVHDRDLKFSYAAVKQLEGKYLIQNRVTGDIYESAQFLYMMVAACL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEHHCCCHHHHHHHHHHHHHHHHHH
FSNYEPAIRLSYVRRFYDAVSQFKISLPTPIMAGVRTPTRQFSSCVLIETGDSLDSINAT
HCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCHHHHCCEEEEECCCCCCCCCCH
SSAIVKYVSQRAGIGINAGRIRALGSAIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPVWHLEVESLLVLKNNRGIDSNRIRHLDYGVQLSRLFYQRLIDKKNVALFSPSD
CEEEEEEEEHEEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFEDQDEFERLYNLYEGDPDIRQKSISAVELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPIRQSNLCLEIALPTKPLQHIMDEEGEIALCTLSAFNLGAIESLDELEE
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDSLLDYQDYPVKAAENSSMARRTLGIGVINFAYYLAKNGVKYSDGSANNLT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
HKTFEAIQYYLLKASNKLAFERGACPKFNETRYSQGILPLDTYKKSLDNICDEQYHLDWE
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCHHHCCCHH
TLRSDIVKHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSVKASKDGILKQVVPEYE
HHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
KYKQNYELLWDIPNNQGYLELVGIMQKFIDQAISANTNYDPKRFSEGRVPMTNLLKDLLV
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHH
AYKMGVKTLYYHNTRDGADDENIIDEADDCAGGACKI
HHHHCCHHEEEECCCCCCCCCHHHCCCHHCCCCCCCC
>Mature Secondary Structure
MKKNLFVTKRNGKRERIDLDKIHRVITWAAEGLKNVSVSQVELNSHIQFYDGIRTDDIHE
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHH
TIIKAAADLISETSPDYQYLAAHLAVFHLRKKAYRRFTPPSIFEHVSKMCSKGKYDMHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
EDYSKQEFEEMEKFIVHDRDLKFSYAAVKQLEGKYLIQNRVTGDIYESAQFLYMMVAACL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEHHCCCHHHHHHHHHHHHHHHHHH
FSNYEPAIRLSYVRRFYDAVSQFKISLPTPIMAGVRTPTRQFSSCVLIETGDSLDSINAT
HCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCHHHHCCEEEEECCCCCCCCCCH
SSAIVKYVSQRAGIGINAGRIRALGSAIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPVWHLEVESLLVLKNNRGIDSNRIRHLDYGVQLSRLFYQRLIDKKNVALFSPSD
CEEEEEEEEHEEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFEDQDEFERLYNLYEGDPDIRQKSISAVELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPIRQSNLCLEIALPTKPLQHIMDEEGEIALCTLSAFNLGAIESLDELEE
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDSLLDYQDYPVKAAENSSMARRTLGIGVINFAYYLAKNGVKYSDGSANNLT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
HKTFEAIQYYLLKASNKLAFERGACPKFNETRYSQGILPLDTYKKSLDNICDEQYHLDWE
HHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCHHHCCCHH
TLRSDIVKHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSVKASKDGILKQVVPEYE
HHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
KYKQNYELLWDIPNNQGYLELVGIMQKFIDQAISANTNYDPKRFSEGRVPMTNLLKDLLV
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHH
AYKMGVKTLYYHNTRDGADDENIIDEADDCAGGACKI
HHHHCCHHEEEECCCCCCCCCHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]