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The map label for this gene is queC

Identifier: 119944857

GI number: 119944857

Start: 1369811

End: 1370476

Strand: Reverse

Name: queC

Synonym: Ping_1099

Alternate gene names: 119944857

Gene position: 1370476-1369811 (Counterclockwise)

Preceding gene: 119944860

Following gene: 119944856

Centisome position: 30.06

GC content: 41.89

Gene sequence:

>666_bases
ATGTCTAAAAAAGTTGTCGTAATTTACTCTGGAGGCATGGATTCTTTCACCGTATTACACAAAGCCATGCAACAAGGTTT
AACCCCTTATGCTTTAACATTTGATTATGGGCAGCGCCATATTAAAGAGATTGAAGTGGCGCGAGAGGTCTGTGAAGAAT
TAGGAGTCCACCACAAAGTTATTGATATTTCAGCCATTAACCAGTTAATTGGCGGTTCTTCATTAACCGACAGCAGCATT
GATGTTGCGCAGGGCCATTACCAACAGGAAAGCATGAAATCCACGGTAGTCCCCAACCGTAATATGATTTTATTATCTTT
GGCTATTGGTTACGCCGTTTCACTCGGTGCCGAACAGGTTTATTACGGTGCACATTCGGGGGATCATGAAATTTATCCCG
ATTGCAGGCCAATTTTTGTTGAAAAAATGAATGACGTCGCTGCCGTGGCTAATTATGAAAAAGTAGAAATTTTTTCGCCC
TATTTAAACAATAACAAAAGCGGTATTTTAAAAGATGGACTGGCAATGGGTCTTGATTATAGTAAAACCTGGACCTGTTA
TAACGGCAGAGAGAAAGCCTGTGGCAAATGCGGATCCTGCGTTGAACGCCTAGAAGCCTTTGCCGCTAACGGCCTTAAGG
ATCCCATTGCATATGAAGACACTTAA

Upstream 100 bases:

>100_bases
ATAGAACAAAAAGATAACAATAAAATTCAAAGGGTTCAAAAACTTATGTATTATTACTCAGGATCCGCTTTATATGTAAA
TCAATTGGGAAAAATGCCTT

Downstream 100 bases:

>100_bases
TTACACCCTCGCTGATGCCAATATAATTAAAGGCATTATAATGATCCCAGTAAATATGAAAGCACAATAATATGCCTGTG
CTGTCCAGCACTTAAAAGCC

Product: exsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSKKVVVIYSGGMDSFTVLHKAMQQGLTPYALTFDYGQRHIKEIEVAREVCEELGVHHKVIDISAINQLIGGSSLTDSSI
DVAQGHYQQESMKSTVVPNRNMILLSLAIGYAVSLGAEQVYYGAHSGDHEIYPDCRPIFVEKMNDVAAVANYEKVEIFSP
YLNNNKSGILKDGLAMGLDYSKTWTCYNGREKACGKCGSCVERLEAFAANGLKDPIAYEDT

Sequences:

>Translated_221_residues
MSKKVVVIYSGGMDSFTVLHKAMQQGLTPYALTFDYGQRHIKEIEVAREVCEELGVHHKVIDISAINQLIGGSSLTDSSI
DVAQGHYQQESMKSTVVPNRNMILLSLAIGYAVSLGAEQVYYGAHSGDHEIYPDCRPIFVEKMNDVAAVANYEKVEIFSP
YLNNNKSGILKDGLAMGLDYSKTWTCYNGREKACGKCGSCVERLEAFAANGLKDPIAYEDT
>Mature_220_residues
SKKVVVIYSGGMDSFTVLHKAMQQGLTPYALTFDYGQRHIKEIEVAREVCEELGVHHKVIDISAINQLIGGSSLTDSSID
VAQGHYQQESMKSTVVPNRNMILLSLAIGYAVSLGAEQVYYGAHSGDHEIYPDCRPIFVEKMNDVAAVANYEKVEIFSPY
LNNNKSGILKDGLAMGLDYSKTWTCYNGREKACGKCGSCVERLEAFAANGLKDPIAYEDT

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=224, Percent_Identity=38.3928571428571, Blast_Score=137, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_PSYIN (A1STX3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942537.1
- ProteinModelPortal:   A1STX3
- SMR:   A1STX3
- STRING:   A1STX3
- GeneID:   4625812
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1099
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- PhylomeDB:   A1STX3
- ProtClustDB:   CLSK932708
- BioCyc:   PING357804:PING_1099-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24302; Mature: 24171

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVVVIYSGGMDSFTVLHKAMQQGLTPYALTFDYGQRHIKEIEVAREVCEELGVHHKV
CCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEE
IDISAINQLIGGSSLTDSSIDVAQGHYQQESMKSTVVPNRNMILLSLAIGYAVSLGAEQV
EEHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCCHHE
YYGAHSGDHEIYPDCRPIFVEKMNDVAAVANYEKVEIFSPYLNNNKSGILKDGLAMGLDY
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCHHHHHHHCCCC
SKTWTCYNGREKACGKCGSCVERLEAFAANGLKDPIAYEDT
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKKVVVIYSGGMDSFTVLHKAMQQGLTPYALTFDYGQRHIKEIEVAREVCEELGVHHKV
CCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEE
IDISAINQLIGGSSLTDSSIDVAQGHYQQESMKSTVVPNRNMILLSLAIGYAVSLGAEQV
EEHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCCHHE
YYGAHSGDHEIYPDCRPIFVEKMNDVAAVANYEKVEIFSPYLNNNKSGILKDGLAMGLDY
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCHHHHHHHCCCC
SKTWTCYNGREKACGKCGSCVERLEAFAANGLKDPIAYEDT
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA