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The map label for this gene is purU [H]

Identifier: 119944860

GI number: 119944860

Start: 1376400

End: 1377236

Strand: Reverse

Name: purU [H]

Synonym: Ping_1102

Alternate gene names: 119944860

Gene position: 1377236-1376400 (Counterclockwise)

Preceding gene: 119944861

Following gene: 119944857

Centisome position: 30.21

GC content: 37.04

Gene sequence:

>837_bases
ATGGAAAAGAAAACACTACTGACGGATTGCCCTGACAGCAAAGGTCTGATTGCTCAAATCACCAATATTTGCCACAAGCA
TCAGTTAAATATTATTAAAAATAATGAATTTGTCGACCACCAGCATGGGCGTTTTTTTATGCGTACTGAAATTGAAGGTT
ATTTCAATGATCAGACGCTATTAGCCGATTTAGATTTTAGCTTACCAAAAGGCTCTCATCGGGCATTAATCTCAGCACAA
AAAAAACGTATTGTCATTTTGGTCACCAAAGAAGCCCATTGTCTTGGTGATATTTTAATGAAAAGTACTTACGGTGGTCT
TGATGTTGAAATTGCAGCTGTTATTGGTAATTACAATACACTCGAAGAGTTAGTGACTAAGTTTAATATCCCCTATCATA
CCGTCAGTCATGAAGGTTTAAATCGTGAAGAGCATGAAAAAAAAGTGCTAGAGGCTATTTCGCCCTATGCGCCTGACTAT
GTGATTCTGGCTAAATATATGCGCATTTTAACGCCTGAATTTGTTAAGGTTTATCAAAATAAACTGATTAATATTCATCA
CTCATTTCTGCCCGCGTTTATTGGTGCTAAACCCTATCAGCAAGCCTTCGATCGTGGAGTGAAAATTATTGGCGCGACGG
CTCACTTTGTCAATAATGACCTTGATGAAGGACCTATTATTACTCAAGATGTAACCCACATTGATCATTCTTATACTGCT
GATGATTTAGTAAAAGCAGGACGAGATGTAGAAAAATCTGTATTAAGCCGTGCATTACAGCAGGTATTAGATGACAAAAT
TTTTGTTTATGCTAATCGCACCGTGGTTTTTCAATAA

Upstream 100 bases:

>100_bases
ACCACGCAATGACCCCTGTCCCTGCGGCAGCGGTAAAAAACACAAAAAGTGTTGTGCTAAATAACGCAGCGCATTAGTCA
CAACAAAGAAGAGATGAAAA

Downstream 100 bases:

>100_bases
ACAAACGTTCAATCTCAGCAGCGCTAAACCTGAGCAATAATGTAAAAGGAGCCAATGTCAGTTTGGTCAATTATTCGACA
GGTCATACTTTGGTCGCTCT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MEKKTLLTDCPDSKGLIAQITNICHKHQLNIIKNNEFVDHQHGRFFMRTEIEGYFNDQTLLADLDFSLPKGSHRALISAQ
KKRIVILVTKEAHCLGDILMKSTYGGLDVEIAAVIGNYNTLEELVTKFNIPYHTVSHEGLNREEHEKKVLEAISPYAPDY
VILAKYMRILTPEFVKVYQNKLINIHHSFLPAFIGAKPYQQAFDRGVKIIGATAHFVNNDLDEGPIITQDVTHIDHSYTA
DDLVKAGRDVEKSVLSRALQQVLDDKIFVYANRTVVFQ

Sequences:

>Translated_278_residues
MEKKTLLTDCPDSKGLIAQITNICHKHQLNIIKNNEFVDHQHGRFFMRTEIEGYFNDQTLLADLDFSLPKGSHRALISAQ
KKRIVILVTKEAHCLGDILMKSTYGGLDVEIAAVIGNYNTLEELVTKFNIPYHTVSHEGLNREEHEKKVLEAISPYAPDY
VILAKYMRILTPEFVKVYQNKLINIHHSFLPAFIGAKPYQQAFDRGVKIIGATAHFVNNDLDEGPIITQDVTHIDHSYTA
DDLVKAGRDVEKSVLSRALQQVLDDKIFVYANRTVVFQ
>Mature_278_residues
MEKKTLLTDCPDSKGLIAQITNICHKHQLNIIKNNEFVDHQHGRFFMRTEIEGYFNDQTLLADLDFSLPKGSHRALISAQ
KKRIVILVTKEAHCLGDILMKSTYGGLDVEIAAVIGNYNTLEELVTKFNIPYHTVSHEGLNREEHEKKVLEAISPYAPDY
VILAKYMRILTPEFVKVYQNKLINIHHSFLPAFIGAKPYQQAFDRGVKIIGATAHFVNNDLDEGPIITQDVTHIDHSYTA
DDLVKAGRDVEKSVLSRALQQVLDDKIFVYANRTVVFQ

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=70.0361010830325, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=161, Percent_Identity=31.6770186335404, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582400, Length=218, Percent_Identity=26.1467889908257, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31574; Mature: 31574

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKTLLTDCPDSKGLIAQITNICHKHQLNIIKNNEFVDHQHGRFFMRTEIEGYFNDQTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCEECCCCCEEEEEEECCCEECCCEE
LADLDFSLPKGSHRALISAQKKRIVILVTKEAHCLGDILMKSTYGGLDVEIAAVIGNYNT
EEECCCCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCHHH
LEELVTKFNIPYHTVSHEGLNREEHEKKVLEAISPYAPDYVILAKYMRILTPEFVKVYQN
HHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHH
KLINIHHSFLPAFIGAKPYQQAFDRGVKIIGATAHFVNNDLDEGPIITQDVTHIDHSYTA
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHCCCCCCCCEEEHHHHHCCCCCCH
DDLVKAGRDVEKSVLSRALQQVLDDKIFVYANRTVVFQ
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure
MEKKTLLTDCPDSKGLIAQITNICHKHQLNIIKNNEFVDHQHGRFFMRTEIEGYFNDQTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCEECCCCCEEEEEEECCCEECCCEE
LADLDFSLPKGSHRALISAQKKRIVILVTKEAHCLGDILMKSTYGGLDVEIAAVIGNYNT
EEECCCCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCHHH
LEELVTKFNIPYHTVSHEGLNREEHEKKVLEAISPYAPDYVILAKYMRILTPEFVKVYQN
HHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHH
KLINIHHSFLPAFIGAKPYQQAFDRGVKIIGATAHFVNNDLDEGPIITQDVTHIDHSYTA
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHCCCCCCCCEEEHHHHHCCCCCCH
DDLVKAGRDVEKSVLSRALQQVLDDKIFVYANRTVVFQ
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]