BacMap

Definition	Psychromonas ingrahamii 37, complete genome.
Accession	NC_008709
Length	4,559,598

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The map label for this gene is paaG [H]

Identifier: 119944428

GI number: 119944428

Start: 841444

End: 842232

Strand: Direct

Name: paaG [H]

Synonym: Ping_0656

Alternate gene names: 119944428

Gene position: 841444-842232 (Clockwise)

Preceding gene: 119944427

Following gene: 119944429

Centisome position: 18.45

GC content: 44.61

Gene sequence:

>789_bases
ATGGAAAATAGCGTACTGACAATAATAGAAAATGGCGTCTGCCTTATTTCTCTTAATCGACCAAAACAATTAAATAGTTT
TAACCCTGAAATGCACCAAGCATTAATGAGTGCATTAAAAAATGCCAACAGAAATGATGAAGTACGATCCATTGTCATTA
CAGGTACAGGTCGCGCATTTTGTGCGGGACAAGACTTAAACGATCGTAATATTAGCGCAGATCAAGCCAGACCCGATCTC
GGTGAAAGTATTGAGAAATTTTATAATCCGCTGATAAATATGCTTGTTGCAATCAACAAGCCCATTATTGCAGCGGTTAA
CGGCGTGGCGGCAGGTGCAGGTGCAAATCTGGCGCTCGCTTGTGACATTGTGATTGCAGGGAAATCCGCGAGTTTTATTC
AGGCATTCTGCAAACTTGGGCTAGTGCCGGATTCCGGGGGCACATGGACACTGCCGCGATTAATTGGCCCGGCACGGGCA
AAGGCATTAATGCTGCTCGGCAATAAGTTACCCGCGCAGCAGGCTAAAGAGTGGGGCATGATCTGGGAATGCGTTGAAGA
TGACCAACTAATGTCAGAAGCGATGACATTAGCTGATCATCTTGCAACGCAACCAACAACGGGACTCGGTTATATAAAGA
GAGCGCTTAATCACAGTAGTGATAATACATTATCAGAACAACTTGAATTAGAGCGCGACCTGCAACGTCTTGCAGGGCGT
ACTGATGATTACCGTGAAGGTGTTACGGCTTTCTTTGAAAAAAGACAACCGGTCTTTAAAGGGAAATAA

Upstream 100 bases:

>100_bases
CGTCAATTGTTTGTCACATTAGCCGCAAGCAACGATAGAAAAGAAGGCATTGATGCTTTTTTTAACAAGCGTCAACCGAC
CTATAAAGGGAGTTAGATTG

Downstream 100 bases:

>100_bases
ACTGTGGAAAAATTACTATTTAAAACCGTCGCCGTGATAGGCGCGGGTGCAATGGGAGCGGGTATTGCTCAAGTTGCAGC
GCAATCTGGCTATCAAGTTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MENSVLTIIENGVCLISLNRPKQLNSFNPEMHQALMSALKNANRNDEVRSIVITGTGRAFCAGQDLNDRNISADQARPDL
GESIEKFYNPLINMLVAINKPIIAAVNGVAAGAGANLALACDIVIAGKSASFIQAFCKLGLVPDSGGTWTLPRLIGPARA
KALMLLGNKLPAQQAKEWGMIWECVEDDQLMSEAMTLADHLATQPTTGLGYIKRALNHSSDNTLSEQLELERDLQRLAGR
TDDYREGVTAFFEKRQPVFKGK

Sequences:

>Translated_262_residues
MENSVLTIIENGVCLISLNRPKQLNSFNPEMHQALMSALKNANRNDEVRSIVITGTGRAFCAGQDLNDRNISADQARPDL
GESIEKFYNPLINMLVAINKPIIAAVNGVAAGAGANLALACDIVIAGKSASFIQAFCKLGLVPDSGGTWTLPRLIGPARA
KALMLLGNKLPAQQAKEWGMIWECVEDDQLMSEAMTLADHLATQPTTGLGYIKRALNHSSDNTLSEQLELERDLQRLAGR
TDDYREGVTAFFEKRQPVFKGK
>Mature_262_residues
MENSVLTIIENGVCLISLNRPKQLNSFNPEMHQALMSALKNANRNDEVRSIVITGTGRAFCAGQDLNDRNISADQARPDL
GESIEKFYNPLINMLVAINKPIIAAVNGVAAGAGANLALACDIVIAGKSASFIQAFCKLGLVPDSGGTWTLPRLIGPARA
KALMLLGNKLPAQQAKEWGMIWECVEDDQLMSEAMTLADHLATQPTTGLGYIKRALNHSSDNTLSEQLELERDLQRLAGR
TDDYREGVTAFFEKRQPVFKGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=37.0517928286853, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=29.1079812206573, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=29.1079812206573, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=29.1079812206573, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=32.6848249027237, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI221307494, Length=183, Percent_Identity=32.7868852459016, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI221136756, Length=183, Percent_Identity=32.7868852459016, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI221136753, Length=183, Percent_Identity=32.7868852459016, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=203, Percent_Identity=29.064039408867, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI49355787, Length=203, Percent_Identity=29.064039408867, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI25453481, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI51538573, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4757966, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI51538581, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=26.615969581749, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI116235487, Length=183, Percent_Identity=28.4153005464481, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=200, Percent_Identity=31.5, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594471, Length=200, Percent_Identity=31.5, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=199, Percent_Identity=30.1507537688442, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=24.1228070175439, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=24.1228070175439, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=60.3053435114504, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=38.4, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=27.5092936802974, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=28.6458333333333, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790281, Length=189, Percent_Identity=25.3968253968254, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=38.3673469387755, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=37.5494071146245, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=29.8387096774194, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=29.3877551020408, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=196, Percent_Identity=31.6326530612245, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=29.4573643410853, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=26.5873015873016, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=26.4069264069264, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=29.7777777777778, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=174, Percent_Identity=29.8850574712644, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=166, Percent_Identity=27.710843373494, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=36.0323886639676, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=36.0323886639676, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=31.0344827586207, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.2030651340996, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24654903, Length=210, Percent_Identity=29.0476190476191, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357171, Length=226, Percent_Identity=27.8761061946903, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=25.8215962441315, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571730, Length=157, Percent_Identity=34.3949044585987, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571729, Length=157, Percent_Identity=34.3949044585987, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28495; Mature: 28495

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSVLTIIENGVCLISLNRPKQLNSFNPEMHQALMSALKNANRNDEVRSIVITGTGRAF
CCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEE
CAGQDLNDRNISADQARPDLGESIEKFYNPLINMLVAINKPIIAAVNGVAAGAGANLALA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCCCCEEEE
CDIVIAGKSASFIQAFCKLGLVPDSGGTWTLPRLIGPARAKALMLLGNKLPAQQAKEWGM
EEEEEECCCHHHHHHHHHHCCCCCCCCCEEHHHHHCHHHHHHHHHHCCCCCHHHHHHHCH
IWECVEDDQLMSEAMTLADHLATQPTTGLGYIKRALNHSSDNTLSEQLELERDLQRLAGR
HHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
TDDYREGVTAFFEKRQPVFKGK
CCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENSVLTIIENGVCLISLNRPKQLNSFNPEMHQALMSALKNANRNDEVRSIVITGTGRAF
CCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEE
CAGQDLNDRNISADQARPDLGESIEKFYNPLINMLVAINKPIIAAVNGVAAGAGANLALA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCCCCEEEE
CDIVIAGKSASFIQAFCKLGLVPDSGGTWTLPRLIGPARAKALMLLGNKLPAQQAKEWGM
EEEEEECCCHHHHHHHHHHCCCCCCCCCEEHHHHHCHHHHHHHHHHCCCCCHHHHHHHCH
IWECVEDDQLMSEAMTLADHLATQPTTGLGYIKRALNHSSDNTLSEQLELERDLQRLAGR
HHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
TDDYREGVTAFFEKRQPVFKGK
CCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]