BacMap

Definition	Psychromonas ingrahamii 37, complete genome.
Accession	NC_008709
Length	4,559,598

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The map label for this gene is paaF [H]

Identifier: 119944427

GI number: 119944427

Start: 840654

End: 841439

Strand: Direct

Name: paaF [H]

Synonym: Ping_0655

Alternate gene names: 119944427

Gene position: 840654-841439 (Clockwise)

Preceding gene: 119944426

Following gene: 119944428

Centisome position: 18.44

GC content: 43.13

Gene sequence:

>786_bases
TTGACTTTAAATAATGAATCAAATTTTCTTCAATGCTCTGAAAAAAAGGGTGTGCTGTTAATACAATTTACGCATTACGA
AAAACGTAATGCACTTTCAAATGACTGCCTTAATGAACTGGCAGGGGTATTAGATAAAGCCGAGCAACAGAATCATATTG
CTGCGGTCGTGCTAACCGGTGGACAACGTTGTTTTGCTGCAGGTGCAGATCTTAATGAGTTAGCAACGCAAGGTGCTATT
GATACCTGGTTGAACCCCAGACCTGAATTATGGGCGCGTTTAAATAAGTTTAGCAAACCTTTATTAGCCGCTGTGAATGG
TTATGCCTTAGGAGCCGGCCTTGAGTTAGTTCTACTGTGTGATATTGCCATTTCAGGACAAAAAGCCCTGTTTGGTTTGC
CGGAAATAACCTTAGGATTAATACCGGGTGCTGGTGGCACACAACGTTTAGCGCGGACAGTCGGTAAATCTTTGACTAAT
CAAATGGTACTGACTGGGCTTCCGATCACGGCCGAACGCGCTTTACAAGCAGGTCTTATTAGTGAAGTTGTTATTCCTGA
GTTAACACTTGAGCGCACCATCATCATTGCCGAAACAATAGCAAAACGCGCGCCGCTTGCGATAAAAGCTGCCAAACAAT
CTCTGCAGCAAGTTCCTAATAGCAGCTTGGATCAAGGCTTAAAATTCGAGCGTCAATTGTTTGTCACATTAGCCGCAAGC
AACGATAGAAAAGAAGGCATTGATGCTTTTTTTAACAAGCGTCAACCGACCTATAAAGGGAGTTAG

Upstream 100 bases:

>100_bases
GGAAGATGAACAAGTTAATAAAGGTTTTGTTCTTAGCTGCCAGGCTGTACCGACTTCAAATGCAGTCACCGTTGACTTTG
ATGCTTAGGAGGCAGGGATA

Downstream 100 bases:

>100_bases
ATTGATGGAAAATAGCGTACTGACAATAATAGAAAATGGCGTCTGCCTTATTTCTCTTAATCGACCAAAACAATTAAATA
GTTTTAACCCTGAAATGCAC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTLNNESNFLQCSEKKGVLLIQFTHYEKRNALSNDCLNELAGVLDKAEQQNHIAAVVLTGGQRCFAAGADLNELATQGAI
DTWLNPRPELWARLNKFSKPLLAAVNGYALGAGLELVLLCDIAISGQKALFGLPEITLGLIPGAGGTQRLARTVGKSLTN
QMVLTGLPITAERALQAGLISEVVIPELTLERTIIIAETIAKRAPLAIKAAKQSLQQVPNSSLDQGLKFERQLFVTLAAS
NDRKEGIDAFFNKRQPTYKGS

Sequences:

>Translated_261_residues
MTLNNESNFLQCSEKKGVLLIQFTHYEKRNALSNDCLNELAGVLDKAEQQNHIAAVVLTGGQRCFAAGADLNELATQGAI
DTWLNPRPELWARLNKFSKPLLAAVNGYALGAGLELVLLCDIAISGQKALFGLPEITLGLIPGAGGTQRLARTVGKSLTN
QMVLTGLPITAERALQAGLISEVVIPELTLERTIIIAETIAKRAPLAIKAAKQSLQQVPNSSLDQGLKFERQLFVTLAAS
NDRKEGIDAFFNKRQPTYKGS
>Mature_260_residues
TLNNESNFLQCSEKKGVLLIQFTHYEKRNALSNDCLNELAGVLDKAEQQNHIAAVVLTGGQRCFAAGADLNELATQGAID
TWLNPRPELWARLNKFSKPLLAAVNGYALGAGLELVLLCDIAISGQKALFGLPEITLGLIPGAGGTQRLARTVGKSLTNQ
MVLTGLPITAERALQAGLISEVVIPELTLERTIIIAETIAKRAPLAIKAAKQSLQQVPNSSLDQGLKFERQLFVTLAASN
DRKEGIDAFFNKRQPTYKGS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=43.8247011952191, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=38.3783783783784, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=28.2945736434109, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=31.3008130081301, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=29.9212598425197, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=28.2868525896414, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI116235487, Length=160, Percent_Identity=28.125, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=117, Percent_Identity=31.6239316239316, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=199, Percent_Identity=25.1256281407035, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538581, Length=199, Percent_Identity=25.1256281407035, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI25453481, Length=199, Percent_Identity=25.1256281407035, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538573, Length=199, Percent_Identity=25.1256281407035, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221136756, Length=117, Percent_Identity=31.6239316239316, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=117, Percent_Identity=31.6239316239316, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4757968, Length=186, Percent_Identity=27.4193548387097, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI49355787, Length=186, Percent_Identity=27.4193548387097, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI261878539, Length=112, Percent_Identity=36.6071428571429, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=248, Percent_Identity=24.5967741935484, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=248, Percent_Identity=24.5967741935484, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=28.1767955801105, Blast_Score=64, Evalue=9e-11,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=28.1767955801105, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=54.1832669322709, Blast_Score=263, Evalue=7e-72,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=29.4354838709677, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=241, Percent_Identity=32.3651452282158, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=32.183908045977, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790281, Length=264, Percent_Identity=28.4090909090909, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=33.1460674157303, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=26.2948207171315, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=42.2764227642276, Blast_Score=197, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=39.7590361445783, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=31.2775330396476, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17536985, Length=264, Percent_Identity=25.3787878787879, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=165, Percent_Identity=37.5757575757576, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=165, Percent_Identity=37.5757575757576, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=165, Percent_Identity=37.5757575757576, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=248, Percent_Identity=28.2258064516129, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=163, Percent_Identity=33.7423312883436, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=227, Percent_Identity=25.9911894273128, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=216, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=27.3584905660377, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=40.1515151515151, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=40.1515151515151, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=31.1023622047244, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.9693486590038, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=27.7272727272727, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=241, Percent_Identity=26.9709543568465, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21357171, Length=247, Percent_Identity=25.1012145748988, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24654903, Length=175, Percent_Identity=29.7142857142857, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=36.7231638418079, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=36.7231638418079, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=36.7231638418079, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28148; Mature: 28017

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNNESNFLQCSEKKGVLLIQFTHYEKRNALSNDCLNELAGVLDKAEQQNHIAAVVLTG
CCCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GQRCFAAGADLNELATQGAIDTWLNPRPELWARLNKFSKPLLAAVNGYALGAGLELVLLC
CCEEEECCCCHHHHHHHCCHHHCCCCCHHHHHHHHHHCCHHHHHHCCEEECCCCEEEEEE
DIAISGQKALFGLPEITLGLIPGAGGTQRLARTVGKSLTNQMVLTGLPITAERALQAGLI
EEHCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHH
SEVVIPELTLERTIIIAETIAKRAPLAIKAAKQSLQQVPNSSLDQGLKFERQLFVTLAAS
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCEEEEEEEECC
NDRKEGIDAFFNKRQPTYKGS
CCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TLNNESNFLQCSEKKGVLLIQFTHYEKRNALSNDCLNELAGVLDKAEQQNHIAAVVLTG
CCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GQRCFAAGADLNELATQGAIDTWLNPRPELWARLNKFSKPLLAAVNGYALGAGLELVLLC
CCEEEECCCCHHHHHHHCCHHHCCCCCHHHHHHHHHHCCHHHHHHCCEEECCCCEEEEEE
DIAISGQKALFGLPEITLGLIPGAGGTQRLARTVGKSLTNQMVLTGLPITAERALQAGLI
EEHCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHH
SEVVIPELTLERTIIIAETIAKRAPLAIKAAKQSLQQVPNSSLDQGLKFERQLFVTLAAS
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCEEEEEEEECC
NDRKEGIDAFFNKRQPTYKGS
CCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]