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The map label for this gene is rpoH [H]

Identifier: 119944398

GI number: 119944398

Start: 807225

End: 808076

Strand: Direct

Name: rpoH [H]

Synonym: Ping_0626

Alternate gene names: 119944398

Gene position: 807225-808076 (Clockwise)

Preceding gene: 119944397

Following gene: 119944399

Centisome position: 17.7

GC content: 41.2

Gene sequence:

>852_bases
ATGAAGAATACAAACAATTCAATGAGTTTAGTTCCTCAAGGAAGTATTGGAGCTTATGTTCAATCCGTAAACCAATTCTC
TATGCTAAGTGCAGAGGCAGAAAAAGAACTTGCTGAACGTTTGTTTCATGAAGGTGACTTAGCGGCCGCCAAAAAACTGG
TCATGTCTCATTTACGTTTTGTCGTCTATATCGCAAAAAGTTATGCAGGTTACGGTCTGGCCCAGGCAGATTTAATTCAA
GAGGGTAATATTGGCTTAATGAAAGCCGTTAAACGTTTTGATCCGTCAGTGGGTGTGCGTTTAGTCTCTTTTGCTGTGCA
TTGGATTAAAGCAGAAATTCATGAATATGTTATTCGTAACTGGCGCATGGTAAAAATTGCAACCACCAAAGCCCAACGTA
AATTGTTTTTTAATCTGCGTAGAGCCAAAAAACGTCTTGGCTGGTTTAGCCATGATGAAGTTGCCACTGTGGCAGAAAAT
TTAAATGTCAGTACTAAAGATGTCCTGGAAATGGAGTCACGCTTAAACGCGCAGGATCAATCCTTTGATTTAAGCGACAG
TGATGACGATGAAAGCGGATTTGCACCGGTGCAATACCTTGAGGACAAAAGTGCAGATGTTTTTCAGGTAGTCGCCACTG
AGAATACTCAGCTGCACACCAAAAATAAACTTTATTCGGCACTTTCAACGCTTGATGAACGTAGCCAGGATATCGTAAGC
TCGCGCTGGTTAAGTGAACCTAAAGCAACACTGCATGACTTGGCTGCCCGATATGGTATTTCAGCAGAACGTATTCGCCA
GCTAGAAGAAAATGCGATGAAAAAGCTACAATCTAATATGGATTTAGACTAA

Upstream 100 bases:

>100_bases
AGCCTTTCATTATTGTATAAAGATAACTACTTTATATTTATATTAATGAGCGTTAACATTTTATGTATCGGGCACGCGTA
ACAACATATTAAGGGTAATA

Downstream 100 bases:

>100_bases
AAAATAGAAGGTAAGTATCATGCTTACCTTTTTTGTTGGTTTTTTTTATAGTCGCTACCTTAACAAAAGCGCTAAAAGAT
CTCTTTGTCAGTGTAATTAC

Product: RNA polymerase sigma-32 factor RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKNTNNSMSLVPQGSIGAYVQSVNQFSMLSAEAEKELAERLFHEGDLAAAKKLVMSHLRFVVYIAKSYAGYGLAQADLIQ
EGNIGLMKAVKRFDPSVGVRLVSFAVHWIKAEIHEYVIRNWRMVKIATTKAQRKLFFNLRRAKKRLGWFSHDEVATVAEN
LNVSTKDVLEMESRLNAQDQSFDLSDSDDDESGFAPVQYLEDKSADVFQVVATENTQLHTKNKLYSALSTLDERSQDIVS
SRWLSEPKATLHDLAARYGISAERIRQLEENAMKKLQSNMDLD

Sequences:

>Translated_283_residues
MKNTNNSMSLVPQGSIGAYVQSVNQFSMLSAEAEKELAERLFHEGDLAAAKKLVMSHLRFVVYIAKSYAGYGLAQADLIQ
EGNIGLMKAVKRFDPSVGVRLVSFAVHWIKAEIHEYVIRNWRMVKIATTKAQRKLFFNLRRAKKRLGWFSHDEVATVAEN
LNVSTKDVLEMESRLNAQDQSFDLSDSDDDESGFAPVQYLEDKSADVFQVVATENTQLHTKNKLYSALSTLDERSQDIVS
SRWLSEPKATLHDLAARYGISAERIRQLEENAMKKLQSNMDLD
>Mature_283_residues
MKNTNNSMSLVPQGSIGAYVQSVNQFSMLSAEAEKELAERLFHEGDLAAAKKLVMSHLRFVVYIAKSYAGYGLAQADLIQ
EGNIGLMKAVKRFDPSVGVRLVSFAVHWIKAEIHEYVIRNWRMVKIATTKAQRKLFFNLRRAKKRLGWFSHDEVATVAEN
LNVSTKDVLEMESRLNAQDQSFDLSDSDDDESGFAPVQYLEDKSADVFQVVATENTQLHTKNKLYSALSTLDERSQDIVS
SRWLSEPKATLHDLAARYGISAERIRQLEENAMKKLQSNMDLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=61.8374558303887, Blast_Score=354, Evalue=3e-99,
Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=30.7984790874525, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789448, Length=265, Percent_Identity=27.5471698113208, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31996; Mature: 31996

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTNNSMSLVPQGSIGAYVQSVNQFSMLSAEAEKELAERLFHEGDLAAAKKLVMSHLRF
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVYIAKSYAGYGLAQADLIQEGNIGLMKAVKRFDPSVGVRLVSFAVHWIKAEIHEYVIRN
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
WRMVKIATTKAQRKLFFNLRRAKKRLGWFSHDEVATVAENLNVSTKDVLEMESRLNAQDQ
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
SFDLSDSDDDESGFAPVQYLEDKSADVFQVVATENTQLHTKNKLYSALSTLDERSQDIVS
CCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRWLSEPKATLHDLAARYGISAERIRQLEENAMKKLQSNMDLD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNTNNSMSLVPQGSIGAYVQSVNQFSMLSAEAEKELAERLFHEGDLAAAKKLVMSHLRF
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVYIAKSYAGYGLAQADLIQEGNIGLMKAVKRFDPSVGVRLVSFAVHWIKAEIHEYVIRN
HHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
WRMVKIATTKAQRKLFFNLRRAKKRLGWFSHDEVATVAENLNVSTKDVLEMESRLNAQDQ
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
SFDLSDSDDDESGFAPVQYLEDKSADVFQVVATENTQLHTKNKLYSALSTLDERSQDIVS
CCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRWLSEPKATLHDLAARYGISAERIRQLEENAMKKLQSNMDLD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]