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The map label for this gene is menF [H]

Identifier: 119944137

GI number: 119944137

Start: 456785

End: 458083

Strand: Reverse

Name: menF [H]

Synonym: Ping_0355

Alternate gene names: 119944137

Gene position: 458083-456785 (Counterclockwise)

Preceding gene: 119944148

Following gene: 119944136

Centisome position: 10.05

GC content: 41.03

Gene sequence:

>1299_bases
ATGTTAACTGCTGAGCATAATTTCAATTTAATGGTCATTTTTATTCGTGAACAATTACAACAGTTTGCAATAAAACAGGG
CGACAAACCAAGCTGCAATCTGGTTATCCCGCTTACCCCGTTTGATTTACTTGGTTTATTAAAAGGGCAATCATTAGACA
GCGATGCACTTTATCCAAGAATTTACTGGGAGGATATAGAGCATCTGCAAACTATTGCCTGCTTTGGTGCTATTGATGAA
TGCACCTATATTCCCGAGCCCATTGGCGAGGCTTGTTACTTTGGCGGCTTGGCTTTCCAGCAGCAGGGGGTGCAATGGGC
AGATTTCCCCGAAATACTTTTTATTCGCCCAGCCTTAGAATTTACATTAGAACAGCAAAAATTAAGATTAACCTGTCATT
TTAACGGCGCTAACAGTATTGAAAAAAGCTTATTATTAATAAATTGCCTGCAAAAGCCAGTCTCTCCCGATGCGGTCAAC
AATATCGTTGTCTCAAGAGAGGATCTGCCGAATCGACAACAATGGGCTGAGCTGGTTGAATCGGCCATTGAGTATAAGGC
GTTAATCCCAAAAGTTGTTTTATCAAGGCAGACGGAACTTATCTGTGAGCAGAAAGTTAATCAATGGGATTTAATTAATC
AATGGCAGGAAGCAAACCCAAATAGTTTTCATTTTGCCTTTCAGTTTTCTACAAACCATACTTTTATCGGTTGCTCTCCG
GAACGGCTTTTTTCTCGAGAAAACAACAGACTTAAAACCGAAGCCTTAGCTGGAACAGTCAATCGGGGAAGGGAGCCGCG
TGAAGATGCCATTCTGCGACAAAGTTTGTTAAGTGATAAAAAAATTGACCGGGAAAACTATTTAGTACAAGAATTTATTG
TCTCTAGTCTTAAGCGTTTAAAGGCAGAGGTTACCTGTTCCGAGCCGCATGTCATGCAACTGCTTAATGTACAGCATTTA
CGGGTGGAAATTAATGCTAAATTAAATGCGCAAACCAAGGATGCACATCTTTTACATAATTTACATCCCACCCCGGCTGT
AGGGGGCTGCCCAAAACTCCCCGCGCTGCAGTTTATTAATGATAAAGAGCATTATAATCGTGGTTGGTATGCGGGTACTG
TGGGTTATTTGCGCGTCGATAAAAGTGATTTTAGTGTTGCCATCCGCAGCGCTCTGGTATCAAGCAATAAGATCAAATTA
TTTGCCGGTGCCGGCATTGTGGTAGGTTCAATTGCAGATCAAGAATGGCAGGAATTAGAAAATAAAATTCACACTATTTT
AGATATTCTATCGGTGTAA

Upstream 100 bases:

>100_bases
AAATATCCTAAAAAAGTGTGGTTATACGCGGTTCCGCTAGGAATCGGCTGTTAATCGCGCTACATTAACAAACCTTAATC
CGTCAATATATCATGAGCCC

Downstream 100 bases:

>100_bases
TTTAAGGTGAAAAATGATCACTACATTATATAAAGAGCCGGCTTCATTTGCTCATGAGTTTTCTAATATCAACCTGTTGT
GGGTATCACTGTTTATTGAA

Product: isochorismate synthases

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLTAEHNFNLMVIFIREQLQQFAIKQGDKPSCNLVIPLTPFDLLGLLKGQSLDSDALYPRIYWEDIEHLQTIACFGAIDE
CTYIPEPIGEACYFGGLAFQQQGVQWADFPEILFIRPALEFTLEQQKLRLTCHFNGANSIEKSLLLINCLQKPVSPDAVN
NIVVSREDLPNRQQWAELVESAIEYKALIPKVVLSRQTELICEQKVNQWDLINQWQEANPNSFHFAFQFSTNHTFIGCSP
ERLFSRENNRLKTEALAGTVNRGREPREDAILRQSLLSDKKIDRENYLVQEFIVSSLKRLKAEVTCSEPHVMQLLNVQHL
RVEINAKLNAQTKDAHLLHNLHPTPAVGGCPKLPALQFINDKEHYNRGWYAGTVGYLRVDKSDFSVAIRSALVSSNKIKL
FAGAGIVVGSIADQEWQELENKIHTILDILSV

Sequences:

>Translated_432_residues
MLTAEHNFNLMVIFIREQLQQFAIKQGDKPSCNLVIPLTPFDLLGLLKGQSLDSDALYPRIYWEDIEHLQTIACFGAIDE
CTYIPEPIGEACYFGGLAFQQQGVQWADFPEILFIRPALEFTLEQQKLRLTCHFNGANSIEKSLLLINCLQKPVSPDAVN
NIVVSREDLPNRQQWAELVESAIEYKALIPKVVLSRQTELICEQKVNQWDLINQWQEANPNSFHFAFQFSTNHTFIGCSP
ERLFSRENNRLKTEALAGTVNRGREPREDAILRQSLLSDKKIDRENYLVQEFIVSSLKRLKAEVTCSEPHVMQLLNVQHL
RVEINAKLNAQTKDAHLLHNLHPTPAVGGCPKLPALQFINDKEHYNRGWYAGTVGYLRVDKSDFSVAIRSALVSSNKIKL
FAGAGIVVGSIADQEWQELENKIHTILDILSV
>Mature_432_residues
MLTAEHNFNLMVIFIREQLQQFAIKQGDKPSCNLVIPLTPFDLLGLLKGQSLDSDALYPRIYWEDIEHLQTIACFGAIDE
CTYIPEPIGEACYFGGLAFQQQGVQWADFPEILFIRPALEFTLEQQKLRLTCHFNGANSIEKSLLLINCLQKPVSPDAVN
NIVVSREDLPNRQQWAELVESAIEYKALIPKVVLSRQTELICEQKVNQWDLINQWQEANPNSFHFAFQFSTNHTFIGCSP
ERLFSRENNRLKTEALAGTVNRGREPREDAILRQSLLSDKKIDRENYLVQEFIVSSLKRLKAEVTCSEPHVMQLLNVQHL
RVEINAKLNAQTKDAHLLHNLHPTPAVGGCPKLPALQFINDKEHYNRGWYAGTVGYLRVDKSDFSVAIRSALVSSNKIKL
FAGAGIVVGSIADQEWQELENKIHTILDILSV

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=409, Percent_Identity=33.4963325183374, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786809, Length=274, Percent_Identity=29.5620437956204, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788114, Length=228, Percent_Identity=28.5087719298246, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787518, Length=386, Percent_Identity=25.1295336787565, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=241, Percent_Identity=27.8008298755187, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324361, Length=306, Percent_Identity=24.5098039215686, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49061; Mature: 49061

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAEHNFNLMVIFIREQLQQFAIKQGDKPSCNLVIPLTPFDLLGLLKGQSLDSDALYPR
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCH
IYWEDIEHLQTIACFGAIDECTYIPEPIGEACYFGGLAFQQQGVQWADFPEILFIRPALE
HHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCEEEEEHHHH
FTLEQQKLRLTCHFNGANSIEKSLLLINCLQKPVSPDAVNNIVVSREDLPNRQQWAELVE
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCEEEECCCCCCHHHHHHHHH
SAIEYKALIPKVVLSRQTELICEQKVNQWDLINQWQEANPNSFHFAFQFSTNHTFIGCSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCH
ERLFSRENNRLKTEALAGTVNRGREPREDAILRQSLLSDKKIDRENYLVQEFIVSSLKRL
HHHHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KAEVTCSEPHVMQLLNVQHLRVEINAKLNAQTKDAHLLHNLHPTPAVGGCPKLPALQFIN
HHHCCCCCHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCC
DKEHYNRGWYAGTVGYLRVDKSDFSVAIRSALVSSNKIKLFAGAGIVVGSIADQEWQELE
CHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHH
NKIHTILDILSV
HHHHHHHHHHCC
>Mature Secondary Structure
MLTAEHNFNLMVIFIREQLQQFAIKQGDKPSCNLVIPLTPFDLLGLLKGQSLDSDALYPR
CCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCH
IYWEDIEHLQTIACFGAIDECTYIPEPIGEACYFGGLAFQQQGVQWADFPEILFIRPALE
HHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCEEEEEHHHH
FTLEQQKLRLTCHFNGANSIEKSLLLINCLQKPVSPDAVNNIVVSREDLPNRQQWAELVE
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCEEEECCCCCCHHHHHHHHH
SAIEYKALIPKVVLSRQTELICEQKVNQWDLINQWQEANPNSFHFAFQFSTNHTFIGCSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCH
ERLFSRENNRLKTEALAGTVNRGREPREDAILRQSLLSDKKIDRENYLVQEFIVSSLKRL
HHHHCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KAEVTCSEPHVMQLLNVQHLRVEINAKLNAQTKDAHLLHNLHPTPAVGGCPKLPALQFIN
HHHCCCCCHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCC
DKEHYNRGWYAGTVGYLRVDKSDFSVAIRSALVSSNKIKLFAGAGIVVGSIADQEWQELE
CHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHH
NKIHTILDILSV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]