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The map label for this gene is rfaF [H]

Identifier: 119944119

GI number: 119944119

Start: 434303

End: 435334

Strand: Reverse

Name: rfaF [H]

Synonym: Ping_0334

Alternate gene names: 119944119

Gene position: 435334-434303 (Counterclockwise)

Preceding gene: 119944120

Following gene: 119944118

Centisome position: 9.55

GC content: 43.22

Gene sequence:

>1032_bases
ATGAAAATATTAGTTATCGGTCCTTCTTGGGTCGGCGATATGGTGATGTCGCAATGTTTATATAAATTATTAAAACAGCA
ATATCCCCAGGCACAACTTGATGTTTTGGCACCAAATTGGTGCAATGCATTATTGACCCGCATGCCAGAAGTCGATAACG
CCATCGCCATGCCAATCGGCCATGGCGCGTTGCAGATTGCGCTGCGCTATAAAATGGGCAAACAGTTGGCGGGCGTCTAT
GACTGGGCGATTATCCAGCCTAACTCGTTTAAATCTGCCTTTATTCCTTTTTTCGCGGGAATAAAAAAACGCACTGCATG
GAAAGGTGAATCGCGTTATTTTATTATTAATGATTTACGTAATAATCGCGATAATTTTCCCTTAATGATTGAACGTTACA
GTGCGTTAGCTTTTGATAAAAAAGAGATGACGGATAGCAAAGCGTTACCCGTCATTCCTTTTCCTTCATTAACGGTAAAC
CAGAAAAATCTTGATAATACCCTCAATAAATTAACCCTGTCTGTGGATCAAAAAATCCTAGGATTATGCCCTGGCGCTGA
ATTTGGCCCGTCTAAACGCTGGCCTGACAGTTATTATGCAAAAGTGGCGCAAGAGCGTATTGAAGCAGGCTGGCAAGTGT
GGGTTTTTGGCGGAGAAAAAGACCATCAAGTCGGTGAACTTATTCGCAGTCAGCTTAGCCCGGATTTACAAAAGCAGTGC
ATTAATCTAGCGGGAAAAACAACGTTGTCGGAAGCGGTTGATCTGCTTTCACTGCCTAATATTGTTGTCAGTAATGATTC
TGGTCTAATGCATGTTGCAGCGGCGCATAATCGACCATTGGTTGCCGTATATGGATCGAGCTCTCCAAAATACACTCCAC
CACTTAGCCAACATGTTCGCGTTTTGCATACTGATATAGGGTGCCGCCCCTGCTTTAAGAAGCAATGCAAATTTGGTCAT
CTTAAGTGCTTAACCGAACTTCTCCCTTCACGCGCTACCGCAGCGGTTCATTCATTAATTAAAGAACAGTAA

Upstream 100 bases:

>100_bases
CAAATTTGCGGGCAGCGGGTTATAAAGATACTTTCAAATCTGTTGCACAAGGTACTGCTGAATATATGGCTTGGCTAAAC
AGATAAGTTGAATAAATAAA

Downstream 100 bases:

>100_bases
CAACTTATGCTAAAAAAATGCCTGTTCACAAAAGCGCAATATATCCAGTTTTTTGTTTTTATCTTTTTTTCGATGACTTT
TGCCGTCCGCTCCGGCACTG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKILVIGPSWVGDMVMSQCLYKLLKQQYPQAQLDVLAPNWCNALLTRMPEVDNAIAMPIGHGALQIALRYKMGKQLAGVY
DWAIIQPNSFKSAFIPFFAGIKKRTAWKGESRYFIINDLRNNRDNFPLMIERYSALAFDKKEMTDSKALPVIPFPSLTVN
QKNLDNTLNKLTLSVDQKILGLCPGAEFGPSKRWPDSYYAKVAQERIEAGWQVWVFGGEKDHQVGELIRSQLSPDLQKQC
INLAGKTTLSEAVDLLSLPNIVVSNDSGLMHVAAAHNRPLVAVYGSSSPKYTPPLSQHVRVLHTDIGCRPCFKKQCKFGH
LKCLTELLPSRATAAVHSLIKEQ

Sequences:

>Translated_343_residues
MKILVIGPSWVGDMVMSQCLYKLLKQQYPQAQLDVLAPNWCNALLTRMPEVDNAIAMPIGHGALQIALRYKMGKQLAGVY
DWAIIQPNSFKSAFIPFFAGIKKRTAWKGESRYFIINDLRNNRDNFPLMIERYSALAFDKKEMTDSKALPVIPFPSLTVN
QKNLDNTLNKLTLSVDQKILGLCPGAEFGPSKRWPDSYYAKVAQERIEAGWQVWVFGGEKDHQVGELIRSQLSPDLQKQC
INLAGKTTLSEAVDLLSLPNIVVSNDSGLMHVAAAHNRPLVAVYGSSSPKYTPPLSQHVRVLHTDIGCRPCFKKQCKFGH
LKCLTELLPSRATAAVHSLIKEQ
>Mature_343_residues
MKILVIGPSWVGDMVMSQCLYKLLKQQYPQAQLDVLAPNWCNALLTRMPEVDNAIAMPIGHGALQIALRYKMGKQLAGVY
DWAIIQPNSFKSAFIPFFAGIKKRTAWKGESRYFIINDLRNNRDNFPLMIERYSALAFDKKEMTDSKALPVIPFPSLTVN
QKNLDNTLNKLTLSVDQKILGLCPGAEFGPSKRWPDSYYAKVAQERIEAGWQVWVFGGEKDHQVGELIRSQLSPDLQKQC
INLAGKTTLSEAVDLLSLPNIVVSNDSGLMHVAAAHNRPLVAVYGSSSPKYTPPLSQHVRVLHTDIGCRPCFKKQCKFGH
LKCLTELLPSRATAAVHSLIKEQ

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=346, Percent_Identity=50.2890173410405, Blast_Score=351, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38334; Mature: 38334

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGPSWVGDMVMSQCLYKLLKQQYPQAQLDVLAPNWCNALLTRMPEVDNAIAMPIG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEEEECC
HGALQIALRYKMGKQLAGVYDWAIIQPNSFKSAFIPFFAGIKKRTAWKGESRYFIINDLR
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
NNRDNFPLMIERYSALAFDKKEMTDSKALPVIPFPSLTVNQKNLDNTLNKLTLSVDQKIL
CCCCCCCEEEEHHHHHHCCHHHCCCCCCCCEECCCCCEECHHHHHHHHHHHHHHHHHHHH
GLCPGAEFGPSKRWPDSYYAKVAQERIEAGWQVWVFGGEKDHQVGELIRSQLSPDLQKQC
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHH
INLAGKTTLSEAVDLLSLPNIVVSNDSGLMHVAAAHNRPLVAVYGSSSPKYTPPLSQHVR
HHHHCCHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHH
VLHTDIGCRPCFKKQCKFGHLKCLTELLPSRATAAVHSLIKEQ
HHHHCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVIGPSWVGDMVMSQCLYKLLKQQYPQAQLDVLAPNWCNALLTRMPEVDNAIAMPIG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCEEEEECC
HGALQIALRYKMGKQLAGVYDWAIIQPNSFKSAFIPFFAGIKKRTAWKGESRYFIINDLR
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
NNRDNFPLMIERYSALAFDKKEMTDSKALPVIPFPSLTVNQKNLDNTLNKLTLSVDQKIL
CCCCCCCEEEEHHHHHHCCHHHCCCCCCCCEECCCCCEECHHHHHHHHHHHHHHHHHHHH
GLCPGAEFGPSKRWPDSYYAKVAQERIEAGWQVWVFGGEKDHQVGELIRSQLSPDLQKQC
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHH
INLAGKTTLSEAVDLLSLPNIVVSNDSGLMHVAAAHNRPLVAVYGSSSPKYTPPLSQHVR
HHHHCCHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHH
VLHTDIGCRPCFKKQCKFGHLKCLTELLPSRATAAVHSLIKEQ
HHHHCCCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]