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The map label for this gene is hemB [H]

Identifier: 119944101

GI number: 119944101

Start: 416727

End: 417734

Strand: Reverse

Name: hemB [H]

Synonym: Ping_0316

Alternate gene names: 119944101

Gene position: 417734-416727 (Counterclockwise)

Preceding gene: 119944102

Following gene: 119944098

Centisome position: 9.16

GC content: 45.63

Gene sequence:

>1008_bases
ATGACCATTTCTCAGCACTTCCCTGCGCGACGCTTGCGTCGTTTGCGTAAGCATGATTTTTCTCGTCGTTTAGTTGCCGA
AAACCAGCTTTCTGCAAGCGATCTTATTTATCCAATGTTTGTTGTTGAAGGGAAAAACCAACGCGAAAAGATAAAGTCTA
TGCCGGGTATCGAGCGGCTTTCAATTGATTTACTGGTGGCAGAAGCAAAGCAGTTAGTGGCATTAGGCATTCCAGCTATT
GCCCTGTTTCCGGTGACACCGGACAATATAAAAACCGAGATGGCCGAGGAAGCATACAACCCCGAGGGGCTTGCTCAACG
CGCGGTAAGAAGTGTTAAAGAGGCTTGTCCTGAATTAGGGGTGATCACCGATGTGGCACTGGATCCTTTCACTACCCACG
GACAAGACGGTATTATTGATAAAGAGGGCTATGTATTAAATGACATTACAACCGAGATACTGGTTAAGCAAGCCCTTTCC
CATGCGCAAGCGGGTGCCGATATTGTCGCACCGTCAGATATGATGGATGGTCGTATTGGTGCTATTCGTGAAGCATTGGA
AAATGCCGGATTTATTAATACCCAAATAATGGCTTACAGTGCTAAATATGCGTCAAACTACTACGGCCCTTTTCGGGATG
CCGTCGGCAGCGCCGGCAATTTAAAAGGTGGCAATAAATCAACCTACCAAATGGATCCCGCCAACAGTGATGAAGCAATT
CATGAAGTTGCACTGGATATTCAAGAGGGCGCTGATATGGTCATGGTTAAACCCGGCATGCCCTATTTAGACATAGTCCG
CCGTGTTAAAACTGAACTGCAGGTCCCTACTTTTGCATACCAGGTAAGTGGCGAATATGCGATGCATAAAGCGGCTATAG
ATAATGGTTGGTTAAAAGAGCGCGAAACGGTTTTAGAATCTTTACTTTGTTTTAAACGTGCAGGGGCAAACGGCATTCTG
ACCTACTTTGCCAAGCAGGTCGCTATTTGGTTACAAGAAAATAAATAA

Upstream 100 bases:

>100_bases
CCGGCAGCAAAATGTTGAACAATTTATAGAACAAACAACACAAACCAGCCGAGCCTTTTTTAATATTGGCAGAGCGTTAA
CCGTTTAATAGGATTTATTC

Downstream 100 bases:

>100_bases
TATGCTGTTGGAGCAAGATAAGCTTGCTCCAACCCACTTTTTTATTCCCTCTATTACACTATTTGTAAAGAACAAAAGAT
CAGTATATTTGGCATAAAGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTISQHFPARRLRRLRKHDFSRRLVAENQLSASDLIYPMFVVEGKNQREKIKSMPGIERLSIDLLVAEAKQLVALGIPAI
ALFPVTPDNIKTEMAEEAYNPEGLAQRAVRSVKEACPELGVITDVALDPFTTHGQDGIIDKEGYVLNDITTEILVKQALS
HAQAGADIVAPSDMMDGRIGAIREALENAGFINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKSTYQMDPANSDEAI
HEVALDIQEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIDNGWLKERETVLESLLCFKRAGANGIL
TYFAKQVAIWLQENK

Sequences:

>Translated_335_residues
MTISQHFPARRLRRLRKHDFSRRLVAENQLSASDLIYPMFVVEGKNQREKIKSMPGIERLSIDLLVAEAKQLVALGIPAI
ALFPVTPDNIKTEMAEEAYNPEGLAQRAVRSVKEACPELGVITDVALDPFTTHGQDGIIDKEGYVLNDITTEILVKQALS
HAQAGADIVAPSDMMDGRIGAIREALENAGFINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKSTYQMDPANSDEAI
HEVALDIQEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIDNGWLKERETVLESLLCFKRAGANGIL
TYFAKQVAIWLQENK
>Mature_334_residues
TISQHFPARRLRRLRKHDFSRRLVAENQLSASDLIYPMFVVEGKNQREKIKSMPGIERLSIDLLVAEAKQLVALGIPAIA
LFPVTPDNIKTEMAEEAYNPEGLAQRAVRSVKEACPELGVITDVALDPFTTHGQDGIIDKEGYVLNDITTEILVKQALSH
AQAGADIVAPSDMMDGRIGAIREALENAGFINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKSTYQMDPANSDEAIH
EVALDIQEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIDNGWLKERETVLESLLCFKRAGANGILT
YFAKQVAIWLQENK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=41.025641025641, Blast_Score=231, Evalue=9e-61,
Organism=Escherichia coli, GI87081728, Length=319, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6321398, Length=321, Percent_Identity=37.6947040498442, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=38.6075949367089, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36990; Mature: 36859

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISQHFPARRLRRLRKHDFSRRLVAENQLSASDLIYPMFVVEGKNQREKIKSMPGIERL
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEEECCCHHHHHHCCCCHHHH
SIDLLVAEAKQLVALGIPAIALFPVTPDNIKTEMAEEAYNPEGLAQRAVRSVKEACPELG
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
VITDVALDPFTTHGQDGIIDKEGYVLNDITTEILVKQALSHAQAGADIVAPSDMMDGRIG
CCHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHH
AIREALENAGFINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKSTYQMDPANSDEAI
HHHHHHHHCCCCCHHHEEEEHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEECCCCCHHHH
HEVALDIQEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIDNGWLKE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHHHH
RETVLESLLCFKRAGANGILTYFAKQVAIWLQENK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCC
>Mature Secondary Structure 
TISQHFPARRLRRLRKHDFSRRLVAENQLSASDLIYPMFVVEGKNQREKIKSMPGIERL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEEECCCHHHHHHCCCCHHHH
SIDLLVAEAKQLVALGIPAIALFPVTPDNIKTEMAEEAYNPEGLAQRAVRSVKEACPELG
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
VITDVALDPFTTHGQDGIIDKEGYVLNDITTEILVKQALSHAQAGADIVAPSDMMDGRIG
CCHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHH
AIREALENAGFINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKSTYQMDPANSDEAI
HHHHHHHHCCCCCHHHEEEEHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEECCCCCHHHH
HEVALDIQEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIDNGWLKE
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHHHH
RETVLESLLCFKRAGANGILTYFAKQVAIWLQENK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]