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The map label for this gene is raiA [H]

Identifier: 119944073

GI number: 119944073

Start: 375301

End: 375711

Strand: Reverse

Name: raiA [H]

Synonym: Ping_0288

Alternate gene names: 119944073

Gene position: 375711-375301 (Counterclockwise)

Preceding gene: 119944079

Following gene: 119944068

Centisome position: 8.24

GC content: 35.52

Gene sequence:

>411_bases
ATGAGAATTGAAATCACAAGTAAACAAATTACTATCACTGATACAATGCGCACCAAAGTAGAGGAACGGTTCGAAAAACT
AGAAAAACTACAAATTCCTCTTATCACTCCCCACGTCATTATCACCAAAGAGCCTAAAGGCGTAAAAATAGAAGCCACAA
TTAACGTTCCTAATGATAAGATTTTTGCACATGCACAGAACGATGATTTGTATGTAGCAATTGGACAATTATTTCAAAAA
TTAGAACGTCAGTTGACTAAATACCTCCATAAATCTGAAGCGAGTCGCGCAAAAAATAACGGTAAAGATATGTGCCGAAC
AGGAGAAATTTCAGGCGAAGCGATTGAAGAACCAGAGATTGAAATTGAATCAGATGCTGAAATAAAATTAAATAAAGAGT
TCGCAGCATAA

Upstream 100 bases:

>100_bases
GAAAAGTGTAAAAAATTGTGACAAAGATCGCTTTTAAAGTTGGCAAAAAAGAATAATCTATTTGTAAATGCAATAAACGT
ACAATCAAGAGGTGTCTTAT

Downstream 100 bases:

>100_bases
AACTGATACAAGGCAAGTAAGTAAATAAAAAAGGAGGTTAAAAACCTCCTTTTTTATTACCTGGAAAAAAGGTTATAAAT
TGTTCTTTATTTATCTTCGT

Product: sigma 54 modulation protein/ribosomal protein S30EA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MRIEITSKQITITDTMRTKVEERFEKLEKLQIPLITPHVIITKEPKGVKIEATINVPNDKIFAHAQNDDLYVAIGQLFQK
LERQLTKYLHKSEASRAKNNGKDMCRTGEISGEAIEEPEIEIESDAEIKLNKEFAA

Sequences:

>Translated_136_residues
MRIEITSKQITITDTMRTKVEERFEKLEKLQIPLITPHVIITKEPKGVKIEATINVPNDKIFAHAQNDDLYVAIGQLFQK
LERQLTKYLHKSEASRAKNNGKDMCRTGEISGEAIEEPEIEIESDAEIKLNKEFAA
>Mature_136_residues
MRIEITSKQITITDTMRTKVEERFEKLEKLQIPLITPHVIITKEPKGVKIEATINVPNDKIFAHAQNDDLYVAIGQLFQK
LERQLTKYLHKSEASRAKNNGKDMCRTGEISGEAIEEPEIEIESDAEIKLNKEFAA

Specific function: During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored,

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family [H]

Homologues:

Organism=Escherichia coli, GI1788949, Length=103, Percent_Identity=50.4854368932039, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003489 [H]

Pfam domain/function: PF02482 Ribosomal_S30AE [H]

EC number: NA

Molecular weight: Translated: 15535; Mature: 15535

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEITSKQITITDTMRTKVEERFEKLEKLQIPLITPHVIITKEPKGVKIEATINVPNDK
CEEEEECCEEEEEHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCEEEEEEEECCCCE
IFAHAQNDDLYVAIGQLFQKLERQLTKYLHKSEASRAKNNGKDMCRTGEISGEAIEEPEI
EEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCE
EIESDAEIKLNKEFAA
EECCCCEEEECCCCCC
>Mature Secondary Structure
MRIEITSKQITITDTMRTKVEERFEKLEKLQIPLITPHVIITKEPKGVKIEATINVPNDK
CEEEEECCEEEEEHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCEEEEEEEECCCCE
IFAHAQNDDLYVAIGQLFQKLERQLTKYLHKSEASRAKNNGKDMCRTGEISGEAIEEPEI
EEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCE
EIESDAEIKLNKEFAA
EECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]