Click here to switch to the map view.

The map label for this gene is phoQ [H]

Identifier: 119944066

GI number: 119944066

Start: 367681

End: 369108

Strand: Reverse

Name: phoQ [H]

Synonym: Ping_0281

Alternate gene names: 119944066

Gene position: 369108-367681 (Counterclockwise)

Preceding gene: 119944067

Following gene: 119944065

Centisome position: 8.1

GC content: 42.93

Gene sequence:

>1428_bases
ATGCCCAAAAGAGCGACAAACTTAAATTCCCTTAAAACGCGTTTGATCGTTAGCGCTCTGTTATTCATATTAGTGCTTAT
GCCCTTAATTGGATTGACTCTAAACGATGCATTTAAAGAGCAAGTAAAAAGTGCCAGCAAAAATGAACTCAGTGCTTATG
TTTATTCCGTACTCGCGGTGACTGAGTTTGATAATGGCCAATTACTTATGCCGGATTTATTATTAGAGAATCAATTTAAT
GTTATTCAGTCAGGTTTATATGCATTAATAACGATGCCTAACGTGAAGGACAATGATCCGCGCCTTGAATCTGATTCAAC
AAACTTAGTCTCAGACAGAAAAAAAGAGACAGGAAATACCAGAATAGCCTGGCAATCGGACTCCTTTCTGGGCTTTAAAG
CACCTGCTAACTTACCGCATCCGGCGACGGGTCAAAGTGAATTTTCTGAAATTAAACTTGAGGGAAAACCTTATCTAATC
TATAGCTTTACCGCCAGTTTCGAGCAATCGGCTCAATCTGATGGTGCTGCGGCGCTGCCAATCACCCTCCATATCATTAA
AGATCAGGTCGATTTTCAGCGCCAGGTTGACCTGTTTACCAAACAACTCTGGAGCTGGCTGCTTATCTTAATGGCGCTAT
TGGTTATTGTGCAGATAGTTTGGTTGTTATGGACCTTAAAGCCTCTGGCCAAATTCACAAAAGAGCTCAACGATATTGAA
CAGGGCCGAACACAACAGCTTAATGAGATTTATCCTACTGAATTACAAGCTGTTGCACGGCAACTTAATGCACTGCTTAA
TACCGAACAAAGTCAACGTAAGCGCTACCGTAATGCTCTGTCGGATCTGGCCCACAGTCTAAAGACACCGCTTGCCGTTA
TTCAAAGCCAAAAAGGGCTAAATCACGCTGCAATCGAGCAAATATCGATTATTAACAGTATTATTGGTCATCAATTAAAA
CGTGCTCAAACTGCGGCGGGCGCTTCCTGGCATTTAGGTATTAAGGTTAAGTCCGTGTCAGATAAATTAATCAGAACCCT
GCATAAAATCTATCGGGAGCCACAAATATCAATCAGCCAAAATATCAACAGACAAGCTGTTTTTAAGGGGGATGAGGCAG
ATCTGACCGAAATACTGGGTAATGTATTAGATAACGCCTGCAAAGCCGCAACATCACAGGTGGCATTAACCGTCACCAGC
AATAAAAAGGTTTTAATTATCACGGTTGAAGATGATGGCAAGGGGATTAGCCCCGAGCAACACATCCAAATCTTTGAGCG
GGGAATGCGTGCTGACTCCTACCAGAGCGGACATGGGATAGGTCTTGCTATCGTGCGGGATCTTACGGACAGCTATAATG
GTCAACTCGCGGTTTCAACTTCACAAACGCTTGGAGGGGCCAAATTTGAGTTTCACTTTCGCTTCTGA

Upstream 100 bases:

>100_bases
GAAAAAAACTCGATCCGGATAATGAATATGGACTTATCGAGACATTAAGAGGGCAAGGTTACCGCCTTACTCCGTTGCCA
ACAAGCCAGAGTGATGGCTA

Downstream 100 bases:

>100_bases
AAGTTGCCCGTCGAAAAAATACTAAGCAGGCAATAAACCGGCAAAGAGCAAATTCAAAACAAGTCAATAAACAACGCTAA
AACGTTTGTTTTTCGCCCTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPKRATNLNSLKTRLIVSALLFILVLMPLIGLTLNDAFKEQVKSASKNELSAYVYSVLAVTEFDNGQLLMPDLLLENQFN
VIQSGLYALITMPNVKDNDPRLESDSTNLVSDRKKETGNTRIAWQSDSFLGFKAPANLPHPATGQSEFSEIKLEGKPYLI
YSFTASFEQSAQSDGAAALPITLHIIKDQVDFQRQVDLFTKQLWSWLLILMALLVIVQIVWLLWTLKPLAKFTKELNDIE
QGRTQQLNEIYPTELQAVARQLNALLNTEQSQRKRYRNALSDLAHSLKTPLAVIQSQKGLNHAAIEQISIINSIIGHQLK
RAQTAAGASWHLGIKVKSVSDKLIRTLHKIYREPQISISQNINRQAVFKGDEADLTEILGNVLDNACKAATSQVALTVTS
NKKVLIITVEDDGKGISPEQHIQIFERGMRADSYQSGHGIGLAIVRDLTDSYNGQLAVSTSQTLGGAKFEFHFRF

Sequences:

>Translated_475_residues
MPKRATNLNSLKTRLIVSALLFILVLMPLIGLTLNDAFKEQVKSASKNELSAYVYSVLAVTEFDNGQLLMPDLLLENQFN
VIQSGLYALITMPNVKDNDPRLESDSTNLVSDRKKETGNTRIAWQSDSFLGFKAPANLPHPATGQSEFSEIKLEGKPYLI
YSFTASFEQSAQSDGAAALPITLHIIKDQVDFQRQVDLFTKQLWSWLLILMALLVIVQIVWLLWTLKPLAKFTKELNDIE
QGRTQQLNEIYPTELQAVARQLNALLNTEQSQRKRYRNALSDLAHSLKTPLAVIQSQKGLNHAAIEQISIINSIIGHQLK
RAQTAAGASWHLGIKVKSVSDKLIRTLHKIYREPQISISQNINRQAVFKGDEADLTEILGNVLDNACKAATSQVALTVTS
NKKVLIITVEDDGKGISPEQHIQIFERGMRADSYQSGHGIGLAIVRDLTDSYNGQLAVSTSQTLGGAKFEFHFRF
>Mature_474_residues
PKRATNLNSLKTRLIVSALLFILVLMPLIGLTLNDAFKEQVKSASKNELSAYVYSVLAVTEFDNGQLLMPDLLLENQFNV
IQSGLYALITMPNVKDNDPRLESDSTNLVSDRKKETGNTRIAWQSDSFLGFKAPANLPHPATGQSEFSEIKLEGKPYLIY
SFTASFEQSAQSDGAAALPITLHIIKDQVDFQRQVDLFTKQLWSWLLILMALLVIVQIVWLLWTLKPLAKFTKELNDIEQ
GRTQQLNEIYPTELQAVARQLNALLNTEQSQRKRYRNALSDLAHSLKTPLAVIQSQKGLNHAAIEQISIINSIIGHQLKR
AQTAAGASWHLGIKVKSVSDKLIRTLHKIYREPQISISQNINRQAVFKGDEADLTEILGNVLDNACKAATSQVALTVTSN
KKVLIITVEDDGKGISPEQHIQIFERGMRADSYQSGHGIGLAIVRDLTDSYNGQLAVSTSQTLGGAKFEFHFRF

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=287, Percent_Identity=35.8885017421603, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1788393, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=229, Percent_Identity=24.8908296943231, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52906; Mature: 52775

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRATNLNSLKTRLIVSALLFILVLMPLIGLTLNDAFKEQVKSASKNELSAYVYSVLAV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TEFDNGQLLMPDLLLENQFNVIQSGLYALITMPNVKDNDPRLESDSTNLVSDRKKETGNT
EECCCCCEECHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
RIAWQSDSFLGFKAPANLPHPATGQSEFSEIKLEGKPYLIYSFTASFEQSAQSDGAAALP
EEEEECCCEEEEECCCCCCCCCCCCCHHHHEEECCCCEEEEEECCHHHHHHCCCCCEECE
ITLHIIKDQVDFQRQVDLFTKQLWSWLLILMALLVIVQIVWLLWTLKPLAKFTKELNDIE
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRTQQLNEIYPTELQAVARQLNALLNTEQSQRKRYRNALSDLAHSLKTPLAVIQSQKGL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
NHAAIEQISIINSIIGHQLKRAQTAAGASWHLGIKVKSVSDKLIRTLHKIYREPQISISQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCCCHHC
NINRQAVFKGDEADLTEILGNVLDNACKAATSQVALTVTSNKKVLIITVEDDGKGISPEQ
CCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCHHH
HIQIFERGMRADSYQSGHGIGLAIVRDLTDSYNGQLAVSTSQTLGGAKFEFHFRF
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
PKRATNLNSLKTRLIVSALLFILVLMPLIGLTLNDAFKEQVKSASKNELSAYVYSVLAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TEFDNGQLLMPDLLLENQFNVIQSGLYALITMPNVKDNDPRLESDSTNLVSDRKKETGNT
EECCCCCEECHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
RIAWQSDSFLGFKAPANLPHPATGQSEFSEIKLEGKPYLIYSFTASFEQSAQSDGAAALP
EEEEECCCEEEEECCCCCCCCCCCCCHHHHEEECCCCEEEEEECCHHHHHHCCCCCEECE
ITLHIIKDQVDFQRQVDLFTKQLWSWLLILMALLVIVQIVWLLWTLKPLAKFTKELNDIE
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGRTQQLNEIYPTELQAVARQLNALLNTEQSQRKRYRNALSDLAHSLKTPLAVIQSQKGL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
NHAAIEQISIINSIIGHQLKRAQTAAGASWHLGIKVKSVSDKLIRTLHKIYREPQISISQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHCCCCCCHHC
NINRQAVFKGDEADLTEILGNVLDNACKAATSQVALTVTSNKKVLIITVEDDGKGISPEQ
CCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCHHH
HIQIFERGMRADSYQSGHGIGLAIVRDLTDSYNGQLAVSTSQTLGGAKFEFHFRF
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA