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The map label for this gene is argF [H]

Identifier: 119944016

GI number: 119944016

Start: 291319

End: 292227

Strand: Direct

Name: argF [H]

Synonym: Ping_0230

Alternate gene names: 119944016

Gene position: 291319-292227 (Clockwise)

Preceding gene: 119944015

Following gene: 119944017

Centisome position: 6.39

GC content: 39.27

Gene sequence:

>909_bases
ATGAAAAATTTGCTAACAGGTATGGAAATGACTCAGCAACAGGCGTTAGATCTGATTGCTTTGGGAAAAGATTTAAAAGC
TAATCCGGCTAAATACAGTCAAGCATTAGCGGGTAAAAGTGTTGTTACTTTATTTGAAAAACAATCTTTAAGAACACGTG
TTACTTTTGATATTGGGGTTAATAAATTAGGCGGCCACGTTGTTTATTTAGATCAATCTAACGGTGAAATGGGCAGTCGT
GAAGATGTTAAAGATTTTGCTGCCAATATTTCTCGCTGGGCTGACTGTATTGTAGCGCGTGTTTTTGACCACAAGACCTT
AGTTGAAATGCAGGCTCATTCAAGTGTGCCGGTGATTAACTCATTATGTGATTTGTATCATCCATGCCAGGCCATGGCTG
ATTTTTTAACTATCGCTCAGCAGTATGACGATCTCTCTAAAGTGAAAATTGCTTATATTGGTGATGGCAATAATGTCACT
CATTCGCTGTTTATTGCCGGGGCTATTTTAGGTGCTACCGTGACCGCGGTTTGTCCGGTCGGCCATAGCCCGGATGCGCA
AATATTAAAAATGAGCGAGGAGATTGCCGCTAAAAATGGTGGTAGCATCCACGTCACTGATAATATTGAAGATATCAAAG
GTTATGATGTGGTTTATGGTGATACTTGGCTTTCAATGGGTGATGATACACCGCTTGAACAGATCAAAGCTAAATTTATG
CCATACCAAATCAATCAAGCATTATTAGATTTAACTGGTATTAAACACGTATTACATTGCCAACCAGCCCATCGTGGTTT
AGATATCACCTCTGATGTGATGGATGGCCCTGCTTCGTTAATTTTTGACCAGGCAGAAAATAGAATGCACATTCAAAACG
CTATTATGCTTACCTTAATTAACAAATAA

Upstream 100 bases:

>100_bases
TGGCAAGTTGGAAAAATCCTGAAAATTTAGTATCATTGCTTGCTGGTCAGGCCGTGGGCACAAAGGTCGTGAAGTAGATC
CTTTAAGTAGAGAGTCAGAT

Downstream 100 bases:

>100_bases
ATGCAGACGAAAGGAAAATAATCAATGAGTAAAGTTAAAAAAGTGGTACTCGCATATTCAGGTGGCCTGGATACTTCGGC
AATCATTCCATGGTTAAAAG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKNLLTGMEMTQQQALDLIALGKDLKANPAKYSQALAGKSVVTLFEKQSLRTRVTFDIGVNKLGGHVVYLDQSNGEMGSR
EDVKDFAANISRWADCIVARVFDHKTLVEMQAHSSVPVINSLCDLYHPCQAMADFLTIAQQYDDLSKVKIAYIGDGNNVT
HSLFIAGAILGATVTAVCPVGHSPDAQILKMSEEIAAKNGGSIHVTDNIEDIKGYDVVYGDTWLSMGDDTPLEQIKAKFM
PYQINQALLDLTGIKHVLHCQPAHRGLDITSDVMDGPASLIFDQAENRMHIQNAIMLTLINK

Sequences:

>Translated_302_residues
MKNLLTGMEMTQQQALDLIALGKDLKANPAKYSQALAGKSVVTLFEKQSLRTRVTFDIGVNKLGGHVVYLDQSNGEMGSR
EDVKDFAANISRWADCIVARVFDHKTLVEMQAHSSVPVINSLCDLYHPCQAMADFLTIAQQYDDLSKVKIAYIGDGNNVT
HSLFIAGAILGATVTAVCPVGHSPDAQILKMSEEIAAKNGGSIHVTDNIEDIKGYDVVYGDTWLSMGDDTPLEQIKAKFM
PYQINQALLDLTGIKHVLHCQPAHRGLDITSDVMDGPASLIFDQAENRMHIQNAIMLTLINK
>Mature_302_residues
MKNLLTGMEMTQQQALDLIALGKDLKANPAKYSQALAGKSVVTLFEKQSLRTRVTFDIGVNKLGGHVVYLDQSNGEMGSR
EDVKDFAANISRWADCIVARVFDHKTLVEMQAHSSVPVINSLCDLYHPCQAMADFLTIAQQYDDLSKVKIAYIGDGNNVT
HSLFIAGAILGATVTAVCPVGHSPDAQILKMSEEIAAKNGGSIHVTDNIEDIKGYDVVYGDTWLSMGDDTPLEQIKAKFM
PYQINQALLDLTGIKHVLHCQPAHRGLDITSDVMDGPASLIFDQAENRMHIQNAIMLTLINK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=34.5394736842105, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI18105007, Length=273, Percent_Identity=26.007326007326, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=32.5227963525836, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=31.6109422492401, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI2367364, Length=295, Percent_Identity=27.4576271186441, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=281, Percent_Identity=32.0284697508897, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33042; Mature: 33042

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLTGMEMTQQQALDLIALGKDLKANPAKYSQALAGKSVVTLFEKQSLRTRVTFDIGV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
NKLGGHVVYLDQSNGEMGSREDVKDFAANISRWADCIVARVFDHKTLVEMQAHSSVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
SLCDLYHPCQAMADFLTIAQQYDDLSKVKIAYIGDGNNVTHSLFIAGAILGATVTAVCPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEECC
GHSPDAQILKMSEEIAAKNGGSIHVTDNIEDIKGYDVVYGDTWLSMGDDTPLEQIKAKFM
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHCCCCEEEECCEEEECCCCCCHHHHHHHCC
PYQINQALLDLTGIKHVLHCQPAHRGLDITSDVMDGPASLIFDQAENRMHIQNAIMLTLI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCEEHHHEEEEEEE
NK
CC
>Mature Secondary Structure
MKNLLTGMEMTQQQALDLIALGKDLKANPAKYSQALAGKSVVTLFEKQSLRTRVTFDIGV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
NKLGGHVVYLDQSNGEMGSREDVKDFAANISRWADCIVARVFDHKTLVEMQAHSSVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
SLCDLYHPCQAMADFLTIAQQYDDLSKVKIAYIGDGNNVTHSLFIAGAILGATVTAVCPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEECC
GHSPDAQILKMSEEIAAKNGGSIHVTDNIEDIKGYDVVYGDTWLSMGDDTPLEQIKAKFM
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHCCCCEEEECCEEEECCCCCCHHHHHHHCC
PYQINQALLDLTGIKHVLHCQPAHRGLDITSDVMDGPASLIFDQAENRMHIQNAIMLTLI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCEEHHHEEEEEEE
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10692366; 12644485 [H]