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The map label for this gene is argG

Identifier: 119944017

GI number: 119944017

Start: 292252

End: 293460

Strand: Direct

Name: argG

Synonym: Ping_0231

Alternate gene names: 119944017

Gene position: 292252-293460 (Clockwise)

Preceding gene: 119944016

Following gene: 119944018

Centisome position: 6.41

GC content: 44.67

Gene sequence:

>1209_bases
ATGAGTAAAGTTAAAAAAGTGGTACTCGCATATTCAGGTGGCCTGGATACTTCGGCAATCATTCCATGGTTAAAAGAGAC
CTATGATGATTGTGAAATCATCGCTTTTTGTGCCGATGTTGGTCAGGGCGCTGAAGAGTTAGTGGGATTAGAGGAAAAAG
CATTAGCATCGGGCGCTTCTGAGTGCCATATTGTTGATCTGAAAGAAGAGTTTGTTAAGGATTATATCTATCCGACTATG
GCATCGGGTGCTGTTTATGAGGGTACTTACCTGTTAGGCACCGCAATGGCGCGCCCAATTATTGCTAAAGCGCAGGTTGA
AGTGGCACTTAAAGTCGGTGCTGACGCGTTATGTCATGGTTGTACCGGTAAAGGTAATGACCAAATCCGTTTTGAAAGCT
GTTTTGCCGCATTGGCCCCTGAGTTGCAGGTGATTGCTCCTTGGCGTGAATGGGAAATGGAATCGCGTGAAGATTTACTT
GATTACCTTGCCGAGCGTAATATCGAAACAACAGCAAGTGCGACTAAAATCTATAGTCGTGACGCCAATGCATGGCATAT
TTCTCACGAAGGTGGTGAGCTGGAAAATCCATGGAATGCACCAAGCAAAGGCGTTTGGACATTAACCGTTGATCCTGAAG
ACGCACCTGATAAACCTGAATACTTAACTATTAAAGTTGAAAATGCCCGTGTTGTTGCCGTTGACGGTGTTGAGCTTTCT
CCCTATGACGCACTGATGCTGTTAAATGAAAAAGCGGCAGCGCATGGTGTAGGTCGTGTTGATATCACTGAGAACCGTAC
TGTTGGTATGAAGTCTCGTGGTTGTTACGAAACGCCTGGAGGTACCGTGATGGTAGCTGCGCTGCGCGGTATCGATGAAC
TCGTTCATGACAAACCAGCCCGCAAATGGCGCGACCAAGTGGGTCAGGAATTCACTCACTTAGTCTATGACGGGCGTTGG
TTTACACCACTTTGCGGCTCTTTATTAGCCGCTTCTGAAGCATTGGCAAAAGATGTTAATGGTGAAGTCGTTGTTAAAAT
GTATAAAGGTCAAGTGACGGCAGAGCAAAAACGCTCTCCAAACAGCTTATATTCTGAAGAGTTTGCGACATTTGGTGATG
ATAACGTTTATAATCAAAAACATGCAGAAGGTTTTATCCGTTTATATTCACTTTCAAGCCGTATCCGTACCTTGAACAGT
AAAAAATAA

Upstream 100 bases:

>100_bases
TTCGTTAATTTTTGACCAGGCAGAAAATAGAATGCACATTCAAAACGCTATTATGCTTACCTTAATTAACAAATAAATGC
AGACGAAAGGAAAATAATCA

Downstream 100 bases:

>100_bases
GCATTAAATCTGACATATTACAATTCCCGTCCTGTATTTTCACAGGGCGGGAATTTCTATTTTTATCTGTTTTTTTTTAA
AGTATATAAAAATAGATTCT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSKVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGAEELVGLEEKALASGASECHIVDLKEEFVKDYIYPTM
ASGAVYEGTYLLGTAMARPIIAKAQVEVALKVGADALCHGCTGKGNDQIRFESCFAALAPELQVIAPWREWEMESREDLL
DYLAERNIETTASATKIYSRDANAWHISHEGGELENPWNAPSKGVWTLTVDPEDAPDKPEYLTIKVENARVVAVDGVELS
PYDALMLLNEKAAAHGVGRVDITENRTVGMKSRGCYETPGGTVMVAALRGIDELVHDKPARKWRDQVGQEFTHLVYDGRW
FTPLCGSLLAASEALAKDVNGEVVVKMYKGQVTAEQKRSPNSLYSEEFATFGDDNVYNQKHAEGFIRLYSLSSRIRTLNS
KK

Sequences:

>Translated_402_residues
MSKVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGAEELVGLEEKALASGASECHIVDLKEEFVKDYIYPTM
ASGAVYEGTYLLGTAMARPIIAKAQVEVALKVGADALCHGCTGKGNDQIRFESCFAALAPELQVIAPWREWEMESREDLL
DYLAERNIETTASATKIYSRDANAWHISHEGGELENPWNAPSKGVWTLTVDPEDAPDKPEYLTIKVENARVVAVDGVELS
PYDALMLLNEKAAAHGVGRVDITENRTVGMKSRGCYETPGGTVMVAALRGIDELVHDKPARKWRDQVGQEFTHLVYDGRW
FTPLCGSLLAASEALAKDVNGEVVVKMYKGQVTAEQKRSPNSLYSEEFATFGDDNVYNQKHAEGFIRLYSLSSRIRTLNS
KK
>Mature_401_residues
SKVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGAEELVGLEEKALASGASECHIVDLKEEFVKDYIYPTMA
SGAVYEGTYLLGTAMARPIIAKAQVEVALKVGADALCHGCTGKGNDQIRFESCFAALAPELQVIAPWREWEMESREDLLD
YLAERNIETTASATKIYSRDANAWHISHEGGELENPWNAPSKGVWTLTVDPEDAPDKPEYLTIKVENARVVAVDGVELSP
YDALMLLNEKAAAHGVGRVDITENRTVGMKSRGCYETPGGTVMVAALRGIDELVHDKPARKWRDQVGQEFTHLVYDGRWF
TPLCGSLLAASEALAKDVNGEVVVKMYKGQVTAEQKRSPNSLYSEEFATFGDDNVYNQKHAEGFIRLYSLSSRIRTLNSK
K

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=402, Percent_Identity=44.0298507462687, Blast_Score=301, Evalue=6e-82,
Organism=Homo sapiens, GI16950633, Length=402, Percent_Identity=44.0298507462687, Blast_Score=301, Evalue=6e-82,
Organism=Escherichia coli, GI1789563, Length=363, Percent_Identity=26.9972451790634, Blast_Score=124, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=366, Percent_Identity=44.8087431693989, Blast_Score=292, Evalue=7e-80,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=39.4014962593516, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PSYIN (A1SRI3)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941697.1
- ProteinModelPortal:   A1SRI3
- SMR:   A1SRI3
- STRING:   A1SRI3
- GeneID:   4625791
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0231
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   A1SRI3
- ProtClustDB:   PRK00509
- BioCyc:   PING357804:PING_0231-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44241; Mature: 44110

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 90-90 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 179-179 BINDING 188-188 BINDING 264-264 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGAEELVGLEEKALASGAS
CCCHHEEEEEECCCCCHHHHHHHHHHCCCCHHEEEEEHHHCCCHHHHHCCHHHHHHCCCC
ECHIVDLKEEFVKDYIYPTMASGAVYEGTYLLGTAMARPIIAKAQVEVALKVGADALCHG
CEEEECHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCHHHHHHEEEEEECCCHHHHCC
CTGKGNDQIRFESCFAALAPELQVIAPWREWEMESREDLLDYLAERNIETTASATKIYSR
CCCCCCCCEEHHHHHHHHCCCCEEEECHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCC
DANAWHISHEGGELENPWNAPSKGVWTLTVDPEDAPDKPEYLTIKVENARVVAVDGVELS
CCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCEEC
PYDALMLLNEKAAAHGVGRVDITENRTVGMKSRGCYETPGGTVMVAALRGIDELVHDKPA
CCCEEEEECCHHHHCCCCEEEECCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHH
RKWRDQVGQEFTHLVYDGRWFTPLCGSLLAASEALAKDVNGEVVVKMYKGQVTAEQKRSP
HHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCC
NSLYSEEFATFGDDNVYNQKHAEGFIRLYSLSSRIRTLNSKK
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGAEELVGLEEKALASGAS
CCHHEEEEEECCCCCHHHHHHHHHHCCCCHHEEEEEHHHCCCHHHHHCCHHHHHHCCCC
ECHIVDLKEEFVKDYIYPTMASGAVYEGTYLLGTAMARPIIAKAQVEVALKVGADALCHG
CEEEECHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHCCHHHHHHEEEEEECCCHHHHCC
CTGKGNDQIRFESCFAALAPELQVIAPWREWEMESREDLLDYLAERNIETTASATKIYSR
CCCCCCCCEEHHHHHHHHCCCCEEEECHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCC
DANAWHISHEGGELENPWNAPSKGVWTLTVDPEDAPDKPEYLTIKVENARVVAVDGVELS
CCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCEEC
PYDALMLLNEKAAAHGVGRVDITENRTVGMKSRGCYETPGGTVMVAALRGIDELVHDKPA
CCCEEEEECCHHHHCCCCEEEECCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCHH
RKWRDQVGQEFTHLVYDGRWFTPLCGSLLAASEALAKDVNGEVVVKMYKGQVTAEQKRSP
HHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCC
NSLYSEEFATFGDDNVYNQKHAEGFIRLYSLSSRIRTLNSKK
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA